##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140344_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9301501
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.752905256904235	32.0	18.0	33.0	18.0	33.0
2	27.638542747025454	29.0	25.0	32.0	18.0	33.0
3	27.09396848960184	29.0	25.0	31.0	18.0	33.0
4	30.36487186315413	31.0	29.0	33.0	27.0	33.0
5	31.855360978835566	33.0	32.0	33.0	30.0	33.0
6	35.939381826653566	38.0	36.0	38.0	31.0	38.0
7	36.10812620457709	38.0	37.0	38.0	33.0	38.0
8	36.568626611984456	38.0	37.0	38.0	34.0	38.0
9	36.79386499017739	38.0	38.0	38.0	35.0	38.0
10-11	36.85936017208405	38.0	38.0	38.0	35.0	38.0
12-13	36.922649043417834	38.0	38.0	38.0	35.0	38.0
14-15	36.91787959814228	38.0	38.0	38.0	35.0	38.0
16-17	36.93327517784495	38.0	38.0	38.0	35.0	38.0
18-19	36.94566360848641	38.0	38.0	38.0	35.0	38.0
20-21	36.95474763085535	38.0	38.0	38.0	35.0	38.0
22-23	36.96234719799478	38.0	38.0	38.0	35.0	38.0
24-25	36.96507222387925	38.0	38.0	38.0	35.0	38.0
26-27	36.92019576164359	38.0	38.0	38.0	35.0	38.0
28-29	36.90257820248196	38.0	38.0	38.0	35.0	38.0
30-31	36.8979776554262	38.0	38.0	38.0	35.0	38.0
32-33	36.876588234390205	38.0	38.0	38.0	35.0	38.0
34-35	36.859686837491054	38.0	38.0	38.0	35.0	38.0
36-37	36.8297636392617	38.0	38.0	38.0	35.0	38.0
38-39	36.80175380083058	38.0	38.0	38.0	35.0	38.0
40-41	36.791710351210845	38.0	38.0	38.0	35.0	38.0
42-43	36.750198590966605	38.0	38.0	38.0	35.0	38.0
44-45	36.706361103723324	38.0	38.0	38.0	34.5	38.0
46-47	36.65205057149467	38.0	38.0	38.0	34.0	38.0
48-49	36.609796940481196	38.0	38.0	38.0	34.0	38.0
50-51	36.579155797528585	38.0	38.0	38.0	34.0	38.0
52-53	36.48318490027167	38.0	38.0	38.0	34.0	38.0
54-55	36.519003233006245	38.0	38.0	38.0	34.0	38.0
56-57	36.5064150592935	38.0	38.0	38.0	34.0	38.0
58-59	36.493018762628	38.0	38.0	38.0	34.0	38.0
60-61	36.46244969628904	38.0	38.0	38.0	34.0	38.0
62-63	36.4777602653232	38.0	38.0	38.0	34.0	38.0
64-65	36.48434929494137	38.0	38.0	38.0	34.0	38.0
66-67	36.48070561484221	38.0	38.0	38.0	34.0	38.0
68-69	36.464090655459906	38.0	38.0	38.0	34.0	38.0
70-71	36.4650881098585	38.0	38.0	38.0	34.0	38.0
72-73	36.45204240749648	38.0	38.0	38.0	34.0	38.0
74-75	36.444230206760984	38.0	38.0	38.0	34.0	38.0
76-77	36.42666535958911	38.0	38.0	38.0	34.0	38.0
78-79	36.41326465394295	38.0	38.0	38.0	34.0	38.0
80-81	36.39410160439438	38.0	38.0	38.0	34.0	38.0
82-83	36.37632097551442	38.0	38.0	38.0	34.0	38.0
84-85	36.37770684252973	38.0	38.0	38.0	34.0	38.0
86-87	36.35869037441624	38.0	38.0	38.0	34.0	38.0
88-89	36.33346666280606	38.0	38.0	38.0	33.5	38.0
90-91	36.321564634645156	38.0	37.0	38.0	33.0	38.0
92-93	36.33011638989315	38.0	37.5	38.0	33.5	38.0
94-95	36.31173462986777	38.0	37.0	38.0	33.0	38.0
96-97	36.303528025655595	38.0	37.0	38.0	33.0	38.0
98-99	36.32461462623762	38.0	37.0	38.0	33.5	38.0
100-101	35.78058675433846	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	17.0
20	89.0
21	420.0
22	1771.0
23	5266.0
24	12199.0
25	23363.0
26	39064.0
27	59793.0
28	85449.0
29	114581.0
30	148479.0
31	187604.0
32	238751.0
33	312611.0
34	438997.0
35	714040.0
36	1726047.0
37	5192954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.62381920939427	18.86967490515778	13.673943592544902	21.83256229290305
2	15.07752350937768	20.3132268652124	40.09486210881448	24.51438751659544
3	20.1940216542908	25.443920702136747	29.337523797400745	25.02453384617171
4	13.120968325434786	17.865159612410945	39.29161540701872	29.722256655135553
5	13.906483026713987	38.17281509850417	34.19200016349686	13.728701711284993
6	30.087187003473954	37.554411916958344	18.609415835143167	13.748985244424528
7	26.814424897658988	33.11816017651344	22.440077144538286	17.627337781289278
8	24.600104864795476	36.4948517448958	20.776743452481487	18.12829993782724
9	25.87969404077901	17.26474038975	20.995245821077695	35.8603197483933
10-11	24.474184327884284	26.725632776903424	28.33992599689018	20.46025689832211
12-13	25.35896224485501	24.257183021169542	28.58860245183022	21.79525228214523
14-15	22.577657089968596	25.55435945230775	26.38190330786397	25.486080149859685
16-17	21.90247742890446	28.776192823994585	27.07022567762741	22.25110406947354
18-19	21.91704983513144	27.760746774136063	28.973045800513585	21.349157590218912
20-21	22.763292644465352	26.880369546097928	28.96905951298609	21.38727829645063
22-23	22.46782735790338	26.78518073158362	28.876927958204192	21.870063952308808
24-25	22.249986962316527	26.900181882404933	28.993980084871286	21.855851070407255
26-27	22.146648255190758	27.081059657472778	28.923264720098125	21.849027367238342
28-29	21.990733582011888	27.105243413563585	28.995933672691443	21.908089331733084
30-31	22.39268199970475	26.950835542453945	28.88047057726896	21.776011880572348
32-33	22.184753272751905	27.094665226588642	28.85028035068436	21.870301149975084
34-35	22.178506129772874	27.12986108929086	28.91158699241454	21.78004578852173
36-37	22.246580847440903	27.037776276203857	28.842896556659326	21.872746319695914
38-39	22.062582741383117	27.198375476993323	28.904446347553996	21.834595434069563
40-41	22.321373214900095	27.01778288714204	28.812733916417944	21.848109981539924
42-43	22.208531646557912	27.069261702399235	28.691109689966854	22.031096961076
44-45	22.21308396003973	27.246585428330633	28.527093222881717	22.01323738874792
46-47	22.34305238322728	27.17383141528311	28.248325851569284	22.23479034992032
48-49	22.201964732524313	27.31883677795398	28.288531167359398	22.190667322162305
50-51	22.180551161929532	27.48170359223613	28.181363711069757	22.15638153476458
52-53	22.370669246995547	27.390725306079684	28.040358742874076	22.198246704050693
54-55	22.322818954678137	27.31491523673582	28.081755642634377	22.280510165951668
56-57	22.330664117376973	27.458094337424534	28.018275620247206	22.192965924951288
58-59	22.23898445922957	27.52153577648601	28.13901301508222	22.100466749202194
60-61	22.306232715894055	27.43575133780176	28.13417013640777	22.12384580989642
62-63	22.338792715299892	27.538233296025954	28.05999332654775	22.062980662126407
64-65	22.138400996383794	27.472368344024396	28.082968013425663	22.306262646166143
66-67	22.263605973554366	27.550587389481695	27.892959907426413	22.292846729537523
68-69	22.172609514232295	27.637630907109877	27.995816988139094	22.193942590518738
70-71	22.17943557265404	27.342059383268385	28.088416743332573	22.390088300745003
72-73	22.266407554531533	27.40156519360516	27.84639640248952	22.485630849373788
74-75	22.114736744862494	27.50423249795655	27.88468814635417	22.49634261082679
76-77	22.312754889916583	27.2964896218284	27.89576105392449	22.49499443433053
78-79	22.280066485464463	27.361784771038177	27.808466701560043	22.549682041937317
80-81	22.44236451395224	27.39704988388521	27.64289702373916	22.517688578423392
82-83	22.476887317144836	27.47983808504408	27.75784364577993	22.285430952031156
84-85	22.446410340226784	27.369251253266956	27.65658470714628	22.52775369935998
86-87	22.269500214858294	27.556277321437527	27.711051459989072	22.463171003715114
88-89	22.49885036104808	27.400601038413303	27.665555690405558	22.434992910133055
90-91	22.523456005783206	27.550012856808735	27.700296388034438	22.226234749373614
92-93	22.45669859063846	27.5081374510498	27.634640345198726	22.400523613113013
94-95	22.47467137629753	27.465200145600882	27.65611209451448	22.404016383587113
96-97	22.49256533877353	27.61438278203799	27.706757050284182	22.186294828904302
98-99	22.709685983668294	28.110787755138745	27.789264430712624	21.39026183048034
100-101	23.238846912527446	30.68017778169644	25.16144769119297	20.919527614583146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1869.0
1	1626.0
2	2096.0
3	3199.5
4	4012.5
5	5410.0
6	7210.0
7	9400.5
8	12921.5
9	15596.0
10	15086.0
11	13867.5
12	14462.0
13	17064.5
14	22638.5
15	29576.0
16	35550.0
17	39868.5
18	40596.5
19	37838.0
20	33831.5
21	30011.5
22	28732.5
23	31746.0
24	38296.0
25	48537.0
26	62866.0
27	79045.0
28	95750.0
29	113705.5
30	130942.0
31	149945.0
32	172556.0
33	196340.5
34	219225.5
35	239352.0
36	261950.5
37	283691.0
38	299132.5
39	310001.0
40	323693.5
41	338554.5
42	347431.5
43	355908.5
44	365397.0
45	372742.5
46	380024.0
47	387064.5
48	387793.5
49	382775.5
50	374974.0
51	363905.0
52	347516.0
53	328755.5
54	311213.0
55	291028.0
56	268280.0
57	244135.0
58	219194.0
59	192533.5
60	160899.0
61	129513.5
62	101588.0
63	77247.0
64	58208.0
65	43193.5
66	31659.5
67	23884.0
68	18117.0
69	13332.5
70	9605.0
71	6912.5
72	4708.5
73	3066.5
74	2044.0
75	1251.5
76	770.0
77	457.0
78	276.5
79	170.5
80	116.5
81	81.5
82	63.5
83	49.0
84	37.0
85	29.0
86	26.0
87	24.5
88	21.0
89	17.5
90	11.5
91	6.5
92	4.0
93	3.0
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.988119444377848E-4
4	0.0
5	0.05037896571746861
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.375476495675268E-6
14-15	0.0
16-17	3.225285897405161E-5
18-19	5.375476495675268E-6
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.5351843416460642E-4
44-45	0.0
46-47	0.0031026197877652654
48-49	3.5199522627426465E-4
50-51	2.810420973519708E-5
52-53	8.081358368967133E-4
54-55	0.0011075476475517574
56-57	0.0020501259799559353
58-59	0.004996450223123089
60-61	0.002922024311588074
62-63	0.0055444695163500085
64-65	0.004062133601823244
66-67	0.007546190443278743
68-69	0.0021374065868950214
70-71	0.0025180073325328216
72-73	0.0031861961957418594
74-75	0.0028996749397802897
76-77	8.293248283632994E-4
78-79	3.1341258369959593E-4
80-81	0.0
82-83	6.246329500627194E-5
84-85	0.0011395495530604808
86-87	0.00902513786501034
88-89	0.0022147342299721137
90-91	0.0022089582817559875
92-93	9.126714257700861E-5
94-95	7.173378316338349E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	85.0
20-21	360.0
22-23	923.0
24-25	890.0
26-27	1849.0
28-29	4254.0
30-31	11449.0
32-33	15706.0
34-35	21179.0
36-37	33756.0
38-39	39623.0
40-41	40933.0
42-43	47684.0
44-45	58664.0
46-47	60975.0
48-49	55140.0
50-51	49740.0
52-53	43586.0
54-55	46934.0
56-57	48480.0
58-59	29403.0
60-61	55190.0
62-63	53867.0
64-65	58388.0
66-67	62209.0
68-69	62674.0
70-71	65928.0
72-73	57646.0
74-75	59239.0
76-77	62241.0
78-79	65399.0
80-81	66305.0
82-83	63492.0
84-85	63003.0
86-87	65944.0
88-89	69286.0
90-91	71836.0
92-93	71176.0
94-95	77953.0
96-97	133669.0
98-99	833125.0
100-101	6571318.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.2061297292249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.2030972625163	76.85856807327855
2	6.774632238679573	11.544803867934421
3	1.6183987225269307	4.136924745157246
4	0.6155034716633123	2.0977867462132997
5	0.30176267059263356	1.2856014628976646
6	0.1544640501515462	0.7896770337428487
7	0.0872386620226999	0.5203288127597122
8	0.06570089491914693	0.4478495180645605
9	0.04432783214110887	0.3399302714427565
>10	0.13156833823237649	1.7132643865806965
>50	0.002838266714427909	0.16130960498111363
>100	4.048714885385186E-4	0.058478347100344934
>500	4.181251381850192E-5	0.025473068999230346
>1k	2.0905837561421674E-5	0.020004060847614594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0750952991350536E-5
2	0.0	2.150190598270107E-5	0.0	0.0	1.0750952991350536E-5
3	0.0	2.150190598270107E-5	0.0	0.0	1.0750952991350536E-5
4	0.0	9.675857692215482E-5	0.0	0.0	1.0750952991350536E-5
5	0.0	9.675857692215482E-5	0.0	0.0	1.0750952991350536E-5
6	2.150190598270107E-5	9.675857692215482E-5	0.0	0.0	2.150190598270107E-5
7	2.150190598270107E-5	9.675857692215482E-5	0.0	0.0	2.150190598270107E-5
8	2.150190598270107E-5	1.0750952991350536E-4	0.0	0.0	4.300381196540214E-5
9	2.150190598270107E-5	2.0426810683566018E-4	0.0	0.0	4.300381196540214E-5
10-11	2.6877382478376336E-5	2.0426810683566018E-4	0.0	3.2252858974051605E-5	4.300381196540214E-5
12-13	3.2252858974051605E-5	2.0426810683566018E-4	0.0	6.988119444377848E-5	4.300381196540214E-5
14-15	3.2252858974051605E-5	2.849002542707892E-4	0.0	7.525667093945375E-5	5.913024145242794E-5
16-17	3.2252858974051605E-5	3.0640216025349027E-4	0.0	9.138310042647955E-5	6.450571794810321E-5
18-19	3.2252858974051605E-5	3.1715311324484084E-4	0.0	9.675857692215482E-5	8.063214743512902E-5
20-21	3.2252858974051605E-5	3.2252858974051606E-4	0.0	1.182604829048559E-4	9.138310042647955E-5
22-23	3.2252858974051605E-5	3.2790406623619135E-4	0.0	1.2363595940053117E-4	9.675857692215482E-5
24-25	3.2252858974051605E-5	3.9240978418429457E-4	0.0	1.4513786538323224E-4	9.675857692215482E-5
26-27	3.2252858974051605E-5	4.1928716666267087E-4	0.0	1.881416773486344E-4	9.675857692215482E-5
28-29	3.2252858974051605E-5	4.1928716666267087E-4	0.0	3.655324017059182E-4	9.675857692215482E-5
30-31	3.2252858974051605E-5	5.214212200805009E-4	0.0	0.0010105895811869505	9.675857692215482E-5
32-33	3.2252858974051605E-5	5.375476495675268E-4	0.0	0.002547975858950077	9.675857692215482E-5
34-35	3.2252858974051605E-5	6.181797970026559E-4	0.0	0.004730419316194235	9.675857692215482E-5
36-37	3.2252858974051605E-5	6.988119444377848E-4	0.0	0.007880448542659943	9.675857692215482E-5
38-39	3.2252858974051605E-5	7.686931388815633E-4	0.0	0.01446003177336647	9.675857692215482E-5
40-41	4.300381196540214E-5	8.762026687950687E-4	0.0	0.03702090662571557	9.675857692215482E-5
42-43	4.300381196540214E-5	9.084555277691203E-4	0.0	0.044207918700433405	9.675857692215482E-5
44-45	4.837928846107741E-5	0.0010105895811869505	0.0	0.05330860040761164	9.675857692215482E-5
46-47	5.375476495675268E-5	0.0010750952991350536	0.0	0.06419394031135404	1.0213405341783009E-4
48-49	5.375476495675268E-5	0.001080470775630729	0.0	0.07470299686039919	1.2901143589620642E-4
50-51	5.375476495675268E-5	0.0011503519700745072	0.0	0.08486802291372114	1.2901143589620642E-4
52-53	5.375476495675268E-5	0.001161102923065858	0.0	0.10320377324046946	1.3976238888755696E-4
54-55	5.375476495675268E-5	0.0011772293525528837	0.0	0.14781485267807853	1.5588881837458275E-4
56-57	5.375476495675268E-5	0.0011879803055442342	0.0	0.19622639399812997	1.6126429487025803E-4
58-59	5.375476495675268E-5	0.0011933557820399095	0.0	0.2471912866536272	1.7201524786160857E-4
60-61	6.450571794810321E-5	0.0012148576880226104	0.0	0.5155350733177366	1.7201524786160857E-4
62-63	6.450571794810321E-5	0.001225608641013961	0.0	0.520824542189481	1.827662008529591E-4
64-65	6.450571794810321E-5	0.001343869123918817	0.0	0.5260656317727644	1.881416773486344E-4
66-67	6.450571794810321E-5	0.001343869123918817	0.0	0.5308068020419501	1.988926303399849E-4
68-69	6.450571794810321E-5	0.0013707465063971932	0.0	0.5355103439756659	2.0426810683566018E-4
70-71	6.450571794810321E-5	0.001381497459388544	0.0	0.5401923840033991	2.2577001281836126E-4
72-73	6.450571794810321E-5	0.001386872935884219	0.0	0.5437240720610577	2.2577001281836126E-4
74-75	6.450571794810321E-5	0.0014029993653712448	0.0	0.5464440631678693	2.2577001281836126E-4
76-77	6.450571794810321E-5	0.001440627700840972	0.0	0.5488253992554535	2.3114548931403654E-4
78-79	6.450571794810321E-5	0.0014621296068236729	0.0	0.5511207277191068	2.365209658097118E-4
80-81	6.450571794810321E-5	0.0014621296068236729	0.0	0.5521796965887549	2.4727191880106233E-4
82-83	7.525667093945375E-5	0.0014728805598150235	0.0	0.5525936082789218	2.5802287179241284E-4
84-85	7.525667093945375E-5	0.001521259848276101	0.0	0.5530075199690889	2.687738247837634E-4
86-87	7.525667093945375E-5	0.0015481372307544772	0.0	0.5531204049754981	2.795247777751139E-4
88-89	7.525667093945375E-5	0.0016448958076766321	0.0	0.5531580333109678	2.795247777751139E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13490	0.0	53.496235	1
GTATCAA	24850	0.0	38.584824	1
TCAACGC	28825	0.0	33.340363	4
ATCAACG	28750	0.0	33.120285	3
CAACGCA	29610	0.0	32.396805	5
TATCAAC	29625	0.0	31.843884	2
AACGCAG	30460	0.0	31.548796	6
GTGGTAT	5795	0.0	30.713882	1
TGGTATC	5700	0.0	29.288696	2
ACGCAGA	35290	0.0	27.193314	7
CGCAGAG	36285	0.0	26.496288	8
GCAGAGT	42045	0.0	22.782408	9
GAGTACT	24665	0.0	21.484999	12-13
CAGAGTA	40645	0.0	20.504498	10-11
GTACATG	31010	0.0	20.409273	1
TACATGG	30750	0.0	20.007328	2
ACATGGG	30825	0.0	19.700748	3
GTACTTT	27455	0.0	19.29374	14-15
AGAGTAC	37830	0.0	18.208817	10-11
CATGGGG	22620	0.0	17.650484	4
>>END_MODULE