FastQCFastQC Report
Fri 27 May 2016
SRR2140343_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140343_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9661451
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140400.049.921441
GTATCAA248150.037.396671
TCAACGC284550.032.7218784
ATCAACG285850.032.402213
CAACGCA292100.031.8303685
TATCAAC292500.031.2697262
AACGCAG303100.030.7482156
ACGCAGA353900.026.2842087
CGCAGAG357700.026.0796388
GTGGTAT72400.024.3560091
TATCTCG115900.024.20221594-95
TGGTATC68950.023.766382
GCAGAGT421950.022.0346749
GAGTACT236300.021.46247312-13
CAGAGTA402200.020.09569210-11
GTACATG312700.019.8653321
TACATGG305450.019.8411792
GTACTTT261800.019.35542314-15
ACATGGG312600.019.1879313
AGAGTAC367650.018.1042710-11
Produced by FastQC (version 0.11.5)