FastQCFastQC Report
Fri 27 May 2016
SRR2140342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7559454
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98150.046.4699481
GTATCAA175850.034.5059171
ATCAACG203400.029.1949123
TCAACGC205150.028.9261254
CAACGCA208250.028.4177275
TATCAAC216750.027.6210042
AACGCAG216250.027.441366
ACGCAGA252300.023.4561717
CGCAGAG255150.023.1941668
GTACATG253800.021.673661
TACATGG250150.021.374582
ACATGGG254850.020.4558733
GCAGAGT297450.019.8412829
GTGGTAT50250.019.588191
TGGTATC47500.019.272542
GAGTACT167800.018.10628512-13
CATGGGG167150.018.0540244
CAGAGTA292900.017.45967510-11
CTCGATA7401.546141E-917.42820594
AGAGTAC273700.016.64212610-11
Produced by FastQC (version 0.11.5)