##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140342_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7559454
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04884942748511	33.0	31.0	34.0	30.0	34.0
2	32.18346563124797	33.0	31.0	34.0	30.0	34.0
3	32.08232446417426	33.0	31.0	34.0	30.0	34.0
4	35.967541174269996	37.0	35.0	37.0	35.0	37.0
5	35.83933548639889	37.0	35.0	37.0	35.0	37.0
6	35.900292402070306	37.0	35.0	37.0	35.0	37.0
7	35.85434702030067	37.0	35.0	37.0	35.0	37.0
8	35.84816310807632	37.0	35.0	37.0	35.0	37.0
9	37.59971963583613	39.0	37.0	39.0	35.0	39.0
10-11	37.48556780688129	39.0	37.0	39.0	34.5	39.0
12-13	37.46517923119845	39.0	37.0	39.0	34.0	39.0
14-15	38.75379577678494	40.0	38.0	41.0	34.5	41.0
16-17	38.60579692660343	40.0	38.0	41.0	34.0	41.0
18-19	38.68731286677583	40.0	38.0	41.0	34.0	41.0
20-21	38.72740083776692	40.0	38.0	41.0	34.0	41.0
22-23	38.65358377907489	40.0	38.0	41.0	34.0	41.0
24-25	38.60306583749478	40.0	38.0	41.0	34.0	41.0
26-27	38.39994435269733	40.0	38.0	41.0	34.0	41.0
28-29	38.294871785742494	40.0	38.0	41.0	34.0	41.0
30-31	38.174387399542724	40.0	38.0	41.0	33.5	41.0
32-33	37.988949150236955	40.0	37.0	41.0	33.0	41.0
34-35	38.073876600049836	40.0	37.0	41.0	33.0	41.0
36-37	38.051227492860804	40.0	37.0	41.0	33.0	41.0
38-39	38.00778883159222	40.0	37.0	41.0	33.0	41.0
40-41	37.95182015283024	40.0	37.0	41.0	33.0	41.0
42-43	38.021232679651106	40.0	37.0	41.0	33.0	41.0
44-45	38.03766638764711	40.0	37.0	41.0	33.0	41.0
46-47	37.98492964675836	40.0	37.0	41.0	33.0	41.0
48-49	37.9076153905431	40.0	37.0	41.0	33.0	41.0
50-51	37.79724043423994	40.0	37.0	41.0	33.0	41.0
52-53	37.63178075983319	40.0	37.0	41.0	32.0	41.0
54-55	37.494772162733156	39.5	36.0	41.0	32.0	41.0
56-57	37.36342617404104	39.0	36.0	41.0	31.5	41.0
58-59	37.19974886967992	39.0	35.5	41.0	31.0	41.0
60-61	37.02364123592244	39.0	35.0	41.0	31.0	41.0
62-63	36.822504086812806	38.5	35.0	40.0	31.0	41.0
64-65	36.53973477903628	38.0	35.0	40.0	31.0	41.0
66-67	36.26013555224382	38.0	35.0	40.0	30.5	41.0
68-69	35.95363403833268	37.0	34.0	40.0	30.0	41.0
70-71	35.646134385563585	37.0	34.0	39.0	30.0	41.0
72-73	35.36085630149811	36.0	34.0	39.0	29.5	40.5
74-75	35.03245579406527	36.0	34.0	39.0	29.0	40.0
76-77	33.74136920626315	34.5	32.0	37.0	28.0	39.0
78-79	34.429743821376164	35.0	33.5	37.0	29.0	39.0
80-81	34.33040984814417	35.0	34.0	37.0	29.0	39.0
82-83	34.08651039817778	35.0	34.0	36.0	29.0	38.5
84-85	33.838055364926156	35.0	33.0	36.0	29.0	37.0
86-87	33.624955819035236	35.0	33.0	36.0	29.0	37.0
88-89	33.4487074854634	35.0	33.0	35.0	29.0	36.5
90-91	33.311852841980766	35.0	33.0	35.0	29.0	36.0
92-93	33.250309637908714	35.0	33.0	35.0	29.0	36.0
94-95	33.17465044794034	35.0	33.0	35.0	29.0	36.0
96-97	33.20599524872542	35.0	33.0	35.0	29.0	35.5
98-99	33.32642004284236	35.0	33.0	35.0	29.5	35.0
100	32.13716618107553	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	50.0
21	233.0
22	1136.0
23	3902.0
24	10198.0
25	21654.0
26	39570.0
27	63555.0
28	91272.0
29	122723.0
30	156346.0
31	195185.0
32	242427.0
33	305876.0
34	405716.0
35	552130.0
36	846795.0
37	1445779.0
38	1887251.0
39	1119801.0
40	47850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81988540442207	20.691706041203506	13.648313753876934	23.840094800497496
2	15.072358400487653	21.4842368245114	40.7253222256528	22.71808254934814
3	18.50529636844072	26.169080320262456	30.480651636863914	24.84497167443291
4	12.445607844164407	17.807304601628637	38.951979865212486	30.79510768899447
5	12.824471185352804	38.16659245495772	35.883821768080075	13.125114591609394
6	30.015871516646577	38.14300609541377	18.570838581728257	13.270283806211399
7	26.50616036555021	32.66227957733455	23.0930831777004	17.738476879414836
8	24.5366662724583	36.646390069970664	21.282449764229003	17.53449389334203
9	25.45329331986146	16.41708514927136	21.603028472691282	36.52659305817589
10-11	24.156936466575495	26.95414245526198	28.858049800951235	20.03087127721129
12-13	24.78348303991267	24.433186576702496	29.229690398274794	21.553639985110035
14-15	21.839017738582708	25.97748461727527	26.861291834039864	25.32220581010216
16-17	21.317551016196614	29.2309027377016	27.51415673370935	21.93738951239244
18-19	21.15690446436302	28.370123228582106	29.442397958451355	21.03057434860352
20-21	22.17190825934568	27.47910311414547	29.292350427799875	21.056638198708978
22-23	21.8064448868058	27.542692089596432	29.14547288020019	21.50539014339758
24-25	21.571557550905258	27.685541079301696	29.27471643655056	21.468184933242487
26-27	21.592044457583093	27.873430869700076	29.094493281174728	21.440031391542103
28-29	21.366690391515643	28.006210585256657	29.107217109656357	21.519881913571343
30-31	21.723697234367716	27.909948884496806	29.049095522162972	21.317258358972506
32-33	21.39353808759801	27.961943051165466	29.117823428763316	21.526695432473204
34-35	21.52101177618191	27.92088912366045	29.14469616553538	21.413402934622255
36-37	21.56328425297398	28.100054809424147	28.872787454662884	21.463873482938993
38-39	21.424992744872963	28.05206614170097	29.01263679029639	21.510304323129674
40-41	21.628405673084018	27.919092308759737	28.94504117259008	21.507460845566168
42-43	21.553198835093216	27.957923027632347	28.73495000290377	21.75392813437067
44-45	21.680647123020233	28.16978775331634	28.517944181964307	21.631620941699122
46-47	21.72197598131936	28.17762352602866	28.19665370815516	21.90374678449682
48-49	21.727386645644707	28.236513911494388	28.134399082171303	21.901700360689606
50-51	21.680646196522645	28.329253494208515	28.0349463126501	21.95515399661874
52-53	21.736465410970002	28.264842544460823	28.039837052534327	21.958854992034848
54-55	21.789651248635472	28.321787112241832	27.948628230210094	21.939933408912605
56-57	21.87900478793908	28.313407468461303	27.850827500027176	21.956760243572443
58-59	21.636889333768977	28.378639136440025	28.08155467888009	21.90291685091091
60-61	21.857387216992993	28.52666490277358	27.843244681854546	21.77270319837888
62-63	21.717014228784713	28.612448765638714	27.80132751466771	21.86920949090886
64-65	21.597182286832442	28.48159393432692	27.905716729280382	22.015507049560252
66-67	21.697771103241863	28.502381182133472	27.701642298033345	22.09820541659132
68-69	21.802414120550036	28.421203927318707	27.78935541892019	21.987026533211065
70-71	21.76220991941236	28.204172605809507	27.902095165200453	22.13152230957768
72-73	21.74089719973126	28.223082387883874	27.96547827366325	22.070542138721606
74-75	21.77571322669865	28.30333936316537	27.80602826584511	22.114919144290866
76-77	21.798507615755554	28.234779700542784	27.756042039012385	22.210670644689273
78-79	21.844968054962706	28.200996408001522	27.785553124217067	22.168482412818708
80-81	21.77224060422236	28.21696587511198	27.8325966114073	22.178196909258354
82-83	21.854221926021022	28.163580141709367	27.981232793872053	22.000965138397554
84-85	21.96732952645497	28.108160164891956	27.912957945964568	22.01155236268851
86-87	21.850536782315846	28.265172366198442	27.884850633202703	21.999440218283013
88-89	21.892042581182853	28.118901643863577	27.939944267832296	22.049111507121275
90-91	21.97022598502028	28.05993208052208	27.947153632942605	22.02268830151503
92-93	22.00173803388235	28.07243643468913	27.963210097473713	21.96261543395481
94-95	21.777724606267725	28.097721356547495	28.124292479278225	22.000261557906562
96-97	21.742217257017373	28.26085005994831	28.208163152884673	21.78876953014964
98-99	22.835346891295373	30.14804795872019	28.448551260362464	18.56805388962198
100	28.99032588356898	41.523536395674505	29.486137720756517	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	861.0
1	857.0
2	1643.0
3	2565.5
4	3761.0
5	6821.5
6	9256.5
7	10487.5
8	10978.5
9	10269.0
10	8958.5
11	7671.0
12	8292.5
13	11978.0
14	20718.0
15	32884.5
16	43263.5
17	49701.5
18	51745.5
19	50180.5
20	46647.0
21	42669.0
22	40192.0
23	41559.0
24	47971.0
25	58950.0
26	73712.0
27	90159.5
28	107596.5
29	127383.0
30	146814.5
31	165512.5
32	185170.5
33	204461.0
34	223087.5
35	241451.5
36	259176.5
37	273214.5
38	283799.0
39	293602.5
40	302930.5
41	311332.0
42	317883.0
43	323641.0
44	329662.0
45	336486.5
46	340853.0
47	340864.5
48	335457.5
49	326161.5
50	317792.5
51	306073.0
52	290475.0
53	274006.0
54	255782.0
55	237018.0
56	216265.0
57	192342.5
58	168040.5
59	143243.0
60	118955.0
61	96067.0
62	76017.5
63	58831.5
64	43901.0
65	32910.5
66	24444.0
67	18426.5
68	14041.5
69	10340.5
70	7551.5
71	5398.5
72	3877.0
73	2721.5
74	1835.0
75	1245.5
76	859.5
77	578.5
78	378.0
79	251.5
80	173.0
81	116.5
82	82.0
83	58.5
84	43.0
85	33.5
86	25.0
87	21.5
88	14.0
89	6.0
90	3.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	3.8362558989048684E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.664646944078236E-4
18-19	0.003842870133213325
20-21	0.0
22-23	0.001422233558930446
24-25	5.623798698705911E-4
26-27	0.01354794639407872
28-29	0.007364625663097781
30-31	5.973882980128608E-4
32-33	0.006729040205682108
34-35	0.004960753809638002
36-37	0.002123828506309389
38-39	0.0016205464591604588
40-41	0.004026513871133799
42-43	0.008668782128020993
44-45	0.010297964550879316
46-47	0.008845139005724794
48-49	0.008432652857435627
50-51	0.03463875301077131
52-53	0.012951540062947606
54-55	8.26303307549415E-5
56-57	0.008284917869480924
58-59	0.00683436884026533
60-61	1.481169078958395E-4
62-63	0.0054547014671883914
64-65	0.0025986202365991754
66-67	0.02517994009995447
68-69	0.03381099971913266
70-71	0.01653687886686169
72-73	0.012325372231317036
74-75	0.01805365484498031
76-77	0.002450859825940283
78-79	0.002794462521276923
80-81	0.0016556950764550764
82-83	0.02634393603303397
84-85	9.409025561647336E-4
86-87	0.010802788674779846
88-89	0.0010005503980094193
90-91	0.003164725004327228
92-93	0.0011588078857966105
94-95	9.623455460723437E-4
96-97	0.003202844042047604
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	154.0
20-21	526.0
22-23	1141.0
24-25	2237.0
26-27	3246.0
28-29	13105.0
30-31	26568.0
32-33	33447.0
34-35	47068.0
36-37	69659.0
38-39	78269.0
40-41	81899.0
42-43	88492.0
44-45	101256.0
46-47	99384.0
48-49	89721.0
50-51	76639.0
52-53	71661.0
54-55	76492.0
56-57	81398.0
58-59	82250.0
60-61	81073.0
62-63	80031.0
64-65	83979.0
66-67	88379.0
68-69	87558.0
70-71	83518.0
72-73	78382.0
74-75	79115.0
76-77	80221.0
78-79	84084.0
80-81	85597.0
82-83	82129.0
84-85	82624.0
86-87	87943.0
88-89	94423.0
90-91	100887.0
92-93	110057.0
94-95	125724.0
96-97	271389.0
98-99	1482486.0
100-101	3085243.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.3291540456469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.83001783300902	79.32108619307701
2	6.517759750109662	11.383808904996874
3	1.5828470463171496	4.146860806155826
4	0.544085957407096	1.9005826555391026
5	0.2302099531558257	1.0052020230993128
6	0.11264166135636032	0.5902140597928309
7	0.06181157838528915	0.37785669954294454
8	0.04059954942255722	0.2836419444565083
9	0.020735499016494963	0.16297322290423635
>10	0.05843472184513455	0.7471425721430955
>50	6.544923668635131E-4	0.035577478604434054
>100	1.8052216756152508E-4	0.03153804069245484
>500	2.143544088439062E-5	0.01351539899539159
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3228468616913338E-5	0.0	0.0	0.0
3	0.0	1.3228468616913338E-5	0.0	0.0	0.0
4	0.0	6.614234308456669E-5	0.0	1.3228468616913338E-5	0.0
5	0.0	6.614234308456669E-5	0.0	1.3228468616913338E-5	0.0
6	0.0	6.614234308456669E-5	0.0	2.6456937233826676E-5	0.0
7	0.0	6.614234308456669E-5	0.0	9.259928031839336E-5	0.0
8	0.0	7.937081170148003E-5	0.0	9.259928031839336E-5	0.0
9	0.0	1.058277489353067E-4	0.0	1.5874162340296006E-4	0.0
10-11	0.0	1.058277489353067E-4	0.0	1.7858432632833005E-4	0.0
12-13	0.0	1.256704518606767E-4	0.0	2.1826973217907008E-4	0.0
14-15	0.0	2.3811243510444007E-4	0.0	2.3149820079598342E-4	0.0
16-17	0.0	2.6456937233826675E-4	0.0	2.4472666941289677E-4	0.0
18-19	0.0	2.777978409551801E-4	0.0	3.174832468059201E-4	0.0
20-21	0.0	2.9102630957209344E-4	0.0	0.0010252063178107836	0.0
22-23	0.0	2.9764054388055014E-4	0.0	0.0028639634555617374	0.0
24-25	0.0	3.307117154228334E-4	0.0	0.005661784568038909	0.0
26-27	0.0	3.571686526566601E-4	0.0	0.011912235989530461	0.0
28-29	0.0	3.571686526566601E-4	0.0	0.03426173371780555	0.0
30-31	0.0	4.497679329750535E-4	0.0	0.0375357797004916	0.0
32-33	0.0	4.6299640159196684E-4	0.0	0.043257092377306613	0.0
34-35	0.0	5.159102760596202E-4	0.0	0.05065180633416117	0.0
36-37	0.0	5.423672132934469E-4	0.0	0.06337759314363181	0.0
38-39	0.0	5.688241505272735E-4	0.0	0.09669349135532804	0.0
40-41	0.0	6.415807279202968E-4	0.0	0.13201350256248667	0.0
42-43	0.0	6.481949622287535E-4	0.0	0.15303353919476193	0.0
44-45	0.0	7.209515396217769E-4	0.0	0.17701013856291736	0.0
46-47	0.0	8.069365856317135E-4	0.0	0.20290486588052523	0.0
48-49	0.0	8.267792885570836E-4	0.0	0.2270005214662329	0.0
50-51	0.0	8.995358659501069E-4	0.0	0.2496145885668462	0.0
52-53	0.0	9.127643345670203E-4	0.0	0.3100819186147571	0.0
54-55	0.0	9.19378568875477E-4	0.0	0.38030524426764156	0.0
56-57	0.0	9.39221271800847E-4	0.0	0.44786964772852644	0.0
58-59	0.0	9.39221271800847E-4	0.0	0.4900618483821715	0.0
60-61	0.0	9.458355061093037E-4	0.0	0.517166980578227	0.0
62-63	0.0	9.656782090346737E-4	0.0	0.5374263802650296	0.0
64-65	0.0	0.0010318205521192404	0.0	0.5457470870250682	0.0
66-67	0.0	0.0010516632550446104	0.0	0.5537436963039923	0.0
68-69	0.0	0.001058277489353067	0.0	0.5612574664783991	0.0
70-71	0.0	0.0010648917236615236	0.0	0.5678386296153135	0.0
72-73	0.0	0.0010715059579699804	0.0	0.5731961594051633	0.0
74-75	0.0	0.0010781201922784372	0.0	0.5773432843165658	0.0
76-77	0.0	0.0011111913638207204	0.0	0.5818608063492416	0.0
78-79	0.0	0.0011111913638207204	0.0	0.584645398993102	0.0
80-81	0.0	0.0011111913638207204	0.0	0.585458949813042	0.0
82-83	0.0	0.0011178055981291772	0.0	0.5860410024321863	0.0
84-85	0.0	0.001137648301054547	0.0	0.5862195867585146	0.0
86-87	0.0	0.0011508767696714604	0.0	0.5863518714446837	0.0
88	0.0	0.0011773337069052872	0.0	0.5863518714446837	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9815	0.0	46.469948	1
GTATCAA	17585	0.0	34.505917	1
ATCAACG	20340	0.0	29.194912	3
TCAACGC	20515	0.0	28.926125	4
CAACGCA	20825	0.0	28.417727	5
TATCAAC	21675	0.0	27.621004	2
AACGCAG	21625	0.0	27.44136	6
ACGCAGA	25230	0.0	23.456171	7
CGCAGAG	25515	0.0	23.194166	8
GTACATG	25380	0.0	21.67366	1
TACATGG	25015	0.0	21.37458	2
ACATGGG	25485	0.0	20.455873	3
GCAGAGT	29745	0.0	19.841282	9
GTGGTAT	5025	0.0	19.58819	1
TGGTATC	4750	0.0	19.27254	2
GAGTACT	16780	0.0	18.106285	12-13
CATGGGG	16715	0.0	18.054024	4
CAGAGTA	29290	0.0	17.459675	10-11
CTCGATA	740	1.546141E-9	17.428205	94
AGAGTAC	27370	0.0	16.642126	10-11
>>END_MODULE