FastQCFastQC Report
Fri 27 May 2016
SRR2140341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10835140
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92200.033.781041
GTATCAA150650.029.8898661
ATCAACG158550.026.62163
CAACGCA161550.026.0510715
TCAACGC166500.025.4216864
AACGCAG175250.024.2215146
TATCAAC184200.023.6603892
TACATGG398700.022.2962862
GTACATG416750.022.0924241
ACATGGG409650.021.2259273
TGCGCGA14250.020.91272594
ACGCAGA203850.020.6271157
CTCGATA18550.020.34904194
CGCAGAG207900.020.205568
CATGGGG283900.019.3308894
GTACTAG34850.016.973741
ATGGGGG190900.016.8189075
TCGACGG10650.016.7890994
CTAGTAC38750.016.613963
GAGTACA290500.016.5256021
Produced by FastQC (version 0.11.5)