##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140341_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10835140
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.923354105253832	33.0	31.0	34.0	30.0	34.0
2	32.15609442979048	34.0	31.0	34.0	30.0	34.0
3	32.341235461655316	34.0	31.0	34.0	30.0	34.0
4	35.77185121742774	37.0	35.0	37.0	35.0	37.0
5	35.86727222721626	37.0	35.0	37.0	35.0	37.0
6	35.890575110243155	37.0	36.0	37.0	35.0	37.0
7	35.834115110649236	37.0	36.0	37.0	35.0	37.0
8	35.77153696214354	37.0	36.0	37.0	35.0	37.0
9	37.52461684851326	39.0	38.0	39.0	35.0	39.0
10-11	37.53640502107034	39.0	38.0	39.0	35.0	39.0
12-13	37.457965287019825	39.0	38.0	39.0	35.0	39.0
14-15	38.88198066660883	41.0	39.0	41.0	35.0	41.0
16-17	38.89453052752433	41.0	39.0	41.0	35.0	41.0
18-19	38.919217702770794	41.0	39.0	41.0	35.0	41.0
20-21	38.94854501513643	41.0	39.0	41.0	35.0	41.0
22-23	38.91740944634287	40.0	39.0	41.0	35.0	41.0
24-25	38.878128253714735	40.0	39.0	41.0	35.0	41.0
26-27	38.82110959620103	40.0	38.5	41.0	34.0	41.0
28-29	38.751706013924306	40.0	38.0	41.0	34.0	41.0
30-31	38.63257042130515	40.0	38.0	41.0	34.0	41.0
32-33	38.570962397244884	40.0	38.0	41.0	34.0	41.0
34-35	38.42287608147092	40.0	38.0	41.0	34.0	41.0
36-37	38.3145616127325	40.0	38.0	41.0	33.0	41.0
38-39	38.30752281915689	40.0	38.0	41.0	33.0	41.0
40-41	38.22586413324798	40.0	38.0	41.0	33.0	41.0
42-43	38.05901723290033	40.0	38.0	41.0	33.0	41.0
44-45	38.123732012654216	40.0	38.0	41.0	33.0	41.0
46-47	38.327421276057585	40.0	38.0	41.0	33.0	41.0
48-49	38.31413627218973	40.0	38.0	41.0	33.0	41.0
50-51	38.21406479444681	40.0	38.0	41.0	33.0	41.0
52-53	38.130151245494716	40.0	37.0	41.0	33.0	41.0
54-55	38.041023259883445	40.0	37.0	41.0	33.0	41.0
56-57	38.07261899159741	40.0	37.0	41.0	33.0	41.0
58-59	37.8587204080674	40.0	36.5	41.0	33.0	41.0
60-61	37.771323283684076	39.5	36.0	41.0	33.0	41.0
62-63	37.53790984783295	39.0	35.5	41.0	32.0	41.0
64-65	37.33437671169952	39.0	35.0	41.0	32.0	41.0
66-67	37.0707356509068	39.0	35.0	41.0	32.0	41.0
68-69	36.844621090912185	38.0	35.0	40.5	32.0	41.0
70-71	36.572068921456236	37.0	35.0	40.0	31.5	41.0
72-73	36.25613654787713	37.0	35.0	39.5	31.0	41.0
74-75	35.930369831053355	36.0	35.0	39.0	31.0	41.0
76-77	35.61018929665383	36.0	35.0	39.0	31.0	40.5
78-79	35.26251564804264	35.5	34.0	37.5	31.0	39.5
80-81	34.95614838503444	35.0	34.0	37.0	31.0	39.0
82-83	34.64868159844826	35.0	34.0	37.0	30.0	39.0
84-85	34.42585095792726	35.0	34.0	36.0	30.0	38.0
86-87	34.20640979703035	35.0	34.0	36.0	30.0	37.0
88-89	33.971089148981164	35.0	34.0	36.0	30.0	37.0
90-91	33.79752959904543	35.0	34.0	35.0	30.0	36.5
92-93	33.696992659658875	35.0	34.0	35.0	30.0	36.0
94-95	33.66680917462196	35.0	34.0	35.0	30.0	36.0
96-97	33.61301260152091	35.0	34.0	35.0	30.0	36.0
98-99	33.63854479643982	35.0	34.0	35.0	30.0	36.0
100	32.97466429450339	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	12.0
16	50.0
17	221.0
18	1090.0
19	3541.0
20	8544.0
21	16268.0
22	25777.0
23	36535.0
24	49701.0
25	63715.0
26	74284.0
27	80620.0
28	92452.0
29	111410.0
30	132119.0
31	161000.0
32	211680.0
33	287945.0
34	426326.0
35	647269.0
36	1057927.0
37	1905974.0
38	2892390.0
39	2369890.0
40	178397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.608710178179514	20.986170921649375	13.350293581808817	24.05482531836229
2	14.590576601953389	21.56963890731807	41.234066039870356	22.605718450858177
3	18.060466899678985	26.81944646744132	30.230948400011005	24.889138232868692
4	12.043671322442883	17.909294046741124	39.74428149894273	30.302753131873256
5	12.841011429527665	38.933136400141365	35.71717011728951	12.508682053041447
6	29.247241619960263	38.46739670894864	19.358138009133512	12.927223661957582
7	26.330200131668118	33.141824176633534	23.584533084407425	16.94344260729092
8	24.39884964577079	36.88863664305384	21.787588372701773	16.92492533847359
9	25.51320636019991	17.11412703786415	21.681519660850377	35.69114694108556
10-11	24.149221334205095	27.371533503631046	28.95402487791367	19.525220284250185
12-13	24.431968091368574	24.811807478449346	29.174318565462865	21.581905864719214
14-15	21.748750753544318	26.347998083737316	26.56615501874706	25.337096143971298
16-17	21.48147115801323	29.346983836632916	27.33096431272567	21.840580692628183
18-19	21.469579100504145	29.06498816103066	28.456181599655473	21.009251138809724
20-21	22.578564499499823	28.234093058394592	27.99863455211038	21.188707889995204
22-23	22.341927748813433	28.136058973523532	27.746965581084737	21.775047696578294
24-25	21.99028320617215	28.213991351036512	28.013160662239716	21.78256478055162
26-27	21.9525648913787	28.401064601786985	27.796834281587902	21.84953622524641
28-29	21.66857677850392	28.65312769018485	27.816758617197323	21.86153691411391
30-31	22.154503420540724	28.404882199641584	27.805044713000733	21.635569666816963
32-33	21.9002906593592	28.453455818789337	27.73300147967631	21.913252042175156
34-35	22.023546650761674	28.501434713208845	27.9038455333866	21.57117310264288
36-37	22.001950554862574	28.293279580017415	27.893471929673304	21.811297935446714
38-39	21.836796749549	28.317655755419025	28.04191453217042	21.803632962861556
40-41	21.969686944106048	28.16595930107355	28.05047411821519	21.81387963660521
42-43	21.913463668581233	28.200808369764303	27.925336952020817	21.960391009633646
44-45	21.929966790355124	28.456375832325055	27.819771986568608	21.793885390751218
46-47	21.855947791852156	28.22076270127293	27.82986582584876	22.09342368102616
48-49	21.930865899776407	28.17933622528912	27.968483851453698	21.92131402348077
50-51	21.701337808193838	28.244814658302193	28.018678522866807	22.035169010637162
52-53	21.78936576641874	28.22622931853204	27.866885239319362	22.11751967572986
54-55	21.896019214333016	28.160047746535827	27.929747647315438	22.01418539181571
56-57	22.005368626257404	28.19531457229526	27.76681173441974	22.032505067027593
58-59	21.85853193579308	28.348166294494842	27.957930386796328	21.83537138291575
60-61	21.986864290314774	28.250078970147236	27.90723229803812	21.85582444149987
62-63	21.983828589170574	28.191038411594878	27.767744531390615	22.057388467843936
64-65	21.78282749653561	28.253820874734476	27.91725902254093	22.046092606188985
66-67	22.00657006287412	28.227841740626978	27.68311923242297	22.08246896407593
68-69	21.877659009068488	28.189756919345776	27.868563556220817	22.064020515364923
70-71	21.771127090568747	27.908649848670073	28.006424195162733	22.313798865598443
72-73	21.922516987939662	28.06769271818102	28.047376287985998	21.962414005893322
74-75	21.766845000312706	28.27252562825116	27.959983314237004	22.000646057199134
76-77	21.76102702209064	28.21891703585941	27.80039068543471	22.21966525661524
78-79	21.864375130803772	28.158589938479935	27.81144456238541	22.165590368330886
80-81	21.749686966789906	28.194631890593165	27.92697121406935	22.128709928547575
82-83	21.903377382239167	28.08880286367558	28.136842198010427	21.870977556074827
84-85	22.100102903620193	28.09716524556965	27.87113938601498	21.931592464795177
86-87	21.643527185886988	28.361738973731082	27.991465268185635	22.003268572196298
88-89	21.920619678803973	28.14751800928198	27.922107698482414	22.009754613431635
90-91	21.937904389400632	28.187717296974956	27.9717691278771	21.90260918574731
92-93	21.950968171972285	28.233743783053285	27.90786750929734	21.907420535677087
94-95	21.670564492091266	28.355406926237915	28.0481249548642	21.925903626806615
96-97	21.681957050020014	28.503015665388375	28.13049579533059	21.684531489261015
98-99	22.65123273664924	30.497351937507577	28.223139765100154	18.62827556074303
100	29.020731386656923	41.725099968925115	29.25416864441796	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1992.0
1	1692.5
2	1647.5
3	1894.0
4	1907.5
5	2141.0
6	2656.0
7	3126.0
8	3447.0
9	3816.0
10	4324.0
11	4851.0
12	5424.5
13	6247.5
14	7342.0
15	8668.5
16	10358.0
17	12468.5
18	15252.0
19	18805.5
20	23482.5
21	30397.5
22	39601.0
23	51592.5
24	67400.0
25	86968.5
26	112324.0
27	140559.0
28	168832.5
29	200021.5
30	229145.5
31	255504.0
32	284425.5
33	314942.5
34	345837.5
35	374948.5
36	401169.5
37	425485.5
38	439969.0
39	443970.5
40	450371.0
41	458485.0
42	465783.0
43	472723.5
44	482881.5
45	493528.0
46	496334.0
47	496156.0
48	489037.0
49	475980.0
50	464880.5
51	454330.0
52	438070.0
53	415590.0
54	385525.0
55	349713.0
56	313927.0
57	276743.0
58	242283.0
59	209153.0
60	175794.5
61	143950.5
62	114735.5
63	90234.5
64	68474.0
65	51252.5
66	39404.5
67	31293.0
68	25065.0
69	19298.0
70	15181.5
71	11963.5
72	9346.5
73	7393.5
74	6039.0
75	4986.0
76	3859.5
77	2992.5
78	2101.5
79	1323.0
80	918.5
81	658.5
82	500.5
83	348.0
84	249.0
85	190.0
86	156.0
87	126.5
88	93.5
89	72.5
90	55.5
91	47.5
92	40.5
93	21.5
94	9.0
95	7.5
96	7.0
97	6.5
98	5.5
99	36.5
100	68.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.021974796818499807
3	0.012717879049093966
4	0.005260661145125952
5	0.00931229314988085
6	0.00898004086703079
7	0.0029810413155713722
8	0.008417057832201522
9	0.004725365800534188
10-11	0.003590170500796482
12-13	0.008818529340645344
14-15	0.004693063495257099
16-17	0.00390857893852779
18-19	0.005371411906075972
20-21	0.004164128717927553
22-23	0.009912754365965497
24-25	0.009563331975793168
26-27	0.022594013927886514
28-29	0.01938537835719086
30-31	0.016056494343521778
32-33	0.01221883969860941
34-35	0.009121094089282869
36-37	0.010633631016868775
38-39	0.005279457037597161
40-41	0.006168967044964215
42-43	0.0041815175368955715
44-45	0.007405710333754122
46-47	0.01034085540531747
48-49	0.007750842633083784
50-51	0.01042299476142327
52-53	0.001029658245045313
54-55	0.0028928834438393843
56-57	7.651493188895813E-4
58-59	0.002997411875012669
60-61	0.0011541131608501186
62-63	0.00788356818617959
64-65	0.00432098456333891
66-67	0.004345211710876579
68-69	0.005734852653717536
70-71	8.953830339641758E-4
72-73	6.640485207691046E-4
74-75	0.0
76-77	2.4665257006120004E-5
78-79	5.451432840195883E-4
80-81	2.293628395884084E-4
82-83	0.0
84-85	9.809653871300898E-4
86-87	0.004176550499121249
88-89	0.022908914211752975
90-91	0.0031604568099212168
92-93	0.0015329441405800618
94-95	0.0012965621545820796
96-97	5.878020363099455E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3121.0
20-21	5908.0
22-23	6840.0
24-25	8660.0
26-27	11893.0
28-29	21352.0
30-31	37547.0
32-33	46883.0
34-35	62888.0
36-37	97480.0
38-39	115366.0
40-41	107789.0
42-43	111180.0
44-45	123322.0
46-47	127250.0
48-49	130457.0
50-51	123611.0
52-53	121206.0
54-55	128636.0
56-57	135986.0
58-59	133050.0
60-61	130458.0
62-63	126009.0
64-65	127627.0
66-67	134499.0
68-69	134058.0
70-71	133139.0
72-73	124072.0
74-75	124097.0
76-77	128404.0
78-79	131440.0
80-81	132021.0
82-83	123310.0
84-85	126068.0
86-87	148121.0
88-89	134304.0
90-91	137328.0
92-93	139759.0
94-95	161351.0
96-97	342083.0
98-99	2043952.0
100-101	4392615.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.22827090282259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65425550955159	74.61768665139836
2	6.54179564115057	10.889246796251673
3	1.8789919275110774	4.691557475013261
4	0.802085069063259	2.6702461366042436
5	0.42250336794645227	1.7582112382401125
6	0.23712221411103132	1.184116312386601
7	0.14041222244559382	0.8180386541438487
8	0.0932873177311554	0.621131372154103
9	0.0574702931823005	0.43048378168570295
>10	0.16957441386534683	1.9854444360996666
>50	0.0015803859265971355	0.08922695841524603
>100	8.241676782091226E-4	0.147328377633371
>500	5.848212051512676E-5	0.03652142803240289
>1k	3.898771628161973E-5	0.060760381941374376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.8458460158336672E-5	0.0
4	0.0	9.229230079168336E-6	0.0	2.7687690237505008E-5	0.0
5	0.0	9.229230079168336E-6	0.0	5.5375380475010016E-5	0.0
6	0.0	9.229230079168336E-6	0.0	5.5375380475010016E-5	0.0
7	0.0	9.229230079168336E-6	0.0	5.5375380475010016E-5	0.0
8	0.0	9.229230079168336E-6	0.0	6.460461055417835E-5	0.0
9	0.0	3.6916920316673344E-5	0.0	1.3843845118752504E-4	0.0
10-11	4.614615039584168E-6	3.6916920316673344E-5	0.0	2.999499775729709E-4	0.0
12-13	9.229230079168336E-6	3.6916920316673344E-5	0.0	3.599399730875651E-4	0.0
14-15	9.229230079168336E-6	7.383384063334669E-5	0.0	4.0608612348340677E-4	0.0
16-17	9.229230079168336E-6	7.383384063334669E-5	0.0	4.753053490771693E-4	0.0
18-19	9.229230079168336E-6	7.844845567293086E-5	0.0	5.998999551459418E-4	0.0
20-21	9.229230079168336E-6	8.306307071251503E-5	0.0	8.490891672834869E-4	0.0
22-23	9.229230079168336E-6	8.306307071251503E-5	0.0	0.0011167368395793686	0.0
24-25	9.229230079168336E-6	1.0152153087085169E-4	0.0	0.0015135937329836071	0.0
26-27	1.3843845118752504E-5	1.0152153087085169E-4	0.0	0.002007357542219113	0.0
28-29	1.8458460158336672E-5	1.0152153087085169E-4	0.0	0.005385255751194724	0.0
30-31	1.8458460158336672E-5	1.2459460606877254E-4	0.0	0.018929150892374257	0.0
32-33	1.8458460158336672E-5	1.292092211083567E-4	0.0	0.044014198247553794	0.0
34-35	1.8458460158336672E-5	1.568969113458617E-4	0.0	0.0640923882847845	0.0
36-37	1.8458460158336672E-5	1.845846015833667E-4	0.0	0.09169701545157699	0.0
38-39	1.8458460158336672E-5	1.845846015833667E-4	0.0	0.1223288300843367	0.0
40-41	1.8458460158336672E-5	1.9381383166253505E-4	0.0	0.14949045420732912	0.0
42-43	1.8458460158336672E-5	1.9381383166253505E-4	0.0	0.17463549155802327	0.0
44-45	1.8458460158336672E-5	2.1227229182087172E-4	0.0	0.1976485767604295	0.0
46-47	1.8458460158336672E-5	2.307307519792084E-4	0.0	0.22284437487655906	0.0
48-49	1.8458460158336672E-5	2.307307519792084E-4	0.0	0.24175506730877497	0.0
50-51	1.8458460158336672E-5	2.584184422167134E-4	0.0	0.2590737175523343	0.0
52-53	2.7687690237505008E-5	2.584184422167134E-4	0.0	0.2748603156027518	0.0
54-55	2.7687690237505008E-5	2.584184422167134E-4	0.0	0.2898162829460441	0.0
56-57	2.7687690237505008E-5	2.676476722958817E-4	0.0	0.3044353833914467	0.0
58-59	2.7687690237505008E-5	2.676476722958817E-4	0.0	0.31803465391310126	0.0
60-61	2.7687690237505008E-5	2.676476722958817E-4	0.0	0.3302033937724847	0.0
62-63	2.7687690237505008E-5	2.676476722958817E-4	0.0	0.341786077521841	0.0
64-65	2.7687690237505008E-5	2.7687690237505007E-4	0.0	0.3518551675382136	0.0
66-67	2.7687690237505008E-5	2.8149151741463423E-4	0.0	0.36172121449284456	0.0
68-69	2.7687690237505008E-5	2.9533536253338675E-4	0.0	0.37142574992109006	0.0
70-71	2.7687690237505008E-5	2.9533536253338675E-4	0.0	0.3806503653852188	0.0
72-73	2.7687690237505008E-5	2.9533536253338675E-4	0.0	0.3884213771118786	0.0
74-75	2.7687690237505008E-5	2.9533536253338675E-4	0.0	0.3954171335118882	0.0
76-77	2.7687690237505008E-5	3.0456459261255506E-4	0.0	0.40040091775463904	0.0
78-79	2.7687690237505008E-5	3.0456459261255506E-4	0.0	0.40340503214540835	0.0
80-81	2.7687690237505008E-5	3.0456459261255506E-4	0.0	0.4048863235731149	0.0
82-83	2.7687690237505008E-5	3.0456459261255506E-4	0.0	0.4059199973419817	0.0
84-85	2.7687690237505008E-5	3.0456459261255506E-4	0.0	0.40629839577522764	0.0
86-87	2.7687690237505008E-5	3.0456459261255506E-4	0.0	0.406427604996336	0.0
88	2.7687690237505008E-5	3.2302305277089174E-4	0.0	0.4064368342264152	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9220	0.0	33.78104	1
GTATCAA	15065	0.0	29.889866	1
ATCAACG	15855	0.0	26.6216	3
CAACGCA	16155	0.0	26.051071	5
TCAACGC	16650	0.0	25.421686	4
AACGCAG	17525	0.0	24.221514	6
TATCAAC	18420	0.0	23.660389	2
TACATGG	39870	0.0	22.296286	2
GTACATG	41675	0.0	22.092424	1
ACATGGG	40965	0.0	21.225927	3
TGCGCGA	1425	0.0	20.912725	94
ACGCAGA	20385	0.0	20.627115	7
CTCGATA	1855	0.0	20.349041	94
CGCAGAG	20790	0.0	20.20556	8
CATGGGG	28390	0.0	19.330889	4
GTACTAG	3485	0.0	16.97374	1
ATGGGGG	19090	0.0	16.818907	5
TCGACGG	1065	0.0	16.78909	94
CTAGTAC	3875	0.0	16.61396	3
GAGTACA	29050	0.0	16.525602	1
>>END_MODULE