FastQCFastQC Report
Fri 27 May 2016
SRR2140340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6723697
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53900.040.4421161
GTATCAA92200.032.4092181
TCAACGC100350.028.5706234
ATCAACG102200.028.013473
CAACGCA103050.027.7380055
AACGCAG107900.026.7324126
TATCAAC113600.025.3201162
ACGCAGA127850.022.3952477
GTGTAGA114750.022.03649394
TGTAGAT109850.021.9203994
CGCAGAG131800.021.658678
GGTGTAG131850.021.16250494
GTACATG210300.019.5857811
TACATGG208350.019.026342
GGACCGA10650.018.5671546
ACATGGG213150.018.0905063
GCAGAGT163350.017.2383169
AGGTGTA158850.016.72098494
CATGGGG157000.015.9891174
AGAGTAC143500.015.74327610-11
Produced by FastQC (version 0.11.5)