##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140340_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6723697
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93116138338774	33.0	31.0	34.0	30.0	34.0
2	32.07145592670223	34.0	31.0	34.0	30.0	34.0
3	32.21663290299964	34.0	31.0	34.0	30.0	34.0
4	35.64833558085678	37.0	35.0	37.0	35.0	37.0
5	35.75645868634473	37.0	35.0	37.0	35.0	37.0
6	35.860461737047345	37.0	36.0	37.0	35.0	37.0
7	35.806381370249134	37.0	36.0	37.0	35.0	37.0
8	35.750907722343825	37.0	36.0	37.0	35.0	37.0
9	37.50190497876392	39.0	38.0	39.0	35.0	39.0
10-11	37.48780462593719	39.0	38.0	39.0	35.0	39.0
12-13	37.414207615245004	39.0	38.0	39.0	35.0	39.0
14-15	38.83870755924903	41.0	39.0	41.0	35.0	41.0
16-17	38.855734427057016	41.0	39.0	41.0	35.0	41.0
18-19	38.88240487339034	41.0	39.0	41.0	35.0	41.0
20-21	38.91462950529352	41.0	39.0	41.0	35.0	41.0
22-23	38.88896540402752	40.5	39.0	41.0	35.0	41.0
24-25	38.85135841435994	40.5	39.0	41.0	34.0	41.0
26-27	38.79670215294137	40.0	38.5	41.0	34.0	41.0
28-29	38.725963170273715	40.0	38.0	41.0	34.0	41.0
30-31	38.60639572760513	40.0	38.0	41.0	34.0	41.0
32-33	38.53630030195209	40.0	38.0	41.0	34.0	41.0
34-35	38.381743451646436	40.0	38.0	41.0	33.5	41.0
36-37	38.279491220694666	40.0	38.0	41.0	33.0	41.0
38-39	38.27043592376614	40.0	38.0	41.0	33.0	41.0
40-41	38.18204291942024	40.0	38.0	41.0	33.0	41.0
42-43	38.015902327483005	40.0	38.0	41.0	33.0	41.0
44-45	38.08536037131152	40.0	38.0	41.0	33.0	41.0
46-47	38.290001645310696	40.0	38.0	41.0	33.0	41.0
48-49	38.28433081069701	40.0	38.0	41.0	33.0	41.0
50-51	38.19868388194586	40.0	38.0	41.0	33.0	41.0
52-53	38.12652038592533	40.0	37.0	41.0	33.0	41.0
54-55	38.04759139783401	40.0	37.0	41.0	33.0	41.0
56-57	38.098596117097124	40.0	37.0	41.0	33.0	41.0
58-59	37.911648081499266	40.0	37.0	41.0	33.0	41.0
60-61	37.83296963388273	40.0	36.0	41.0	33.0	41.0
62-63	37.61510940742003	39.0	36.0	41.0	32.0	41.0
64-65	37.412145907977475	39.0	35.5	41.0	32.0	41.0
66-67	37.135806545608105	39.0	35.0	41.0	32.0	41.0
68-69	36.90404964943906	38.0	35.0	40.5	32.0	41.0
70-71	36.61022844151676	37.0	35.0	40.0	31.0	41.0
72-73	36.279402799805425	37.0	35.0	39.5	31.0	41.0
74-75	35.91829045440504	36.5	35.0	39.0	31.0	41.0
76-77	35.563492187021346	36.0	34.5	39.0	31.0	40.5
78-79	35.18665769181759	35.5	34.0	37.5	30.5	39.5
80-81	34.84943261806225	35.0	34.0	37.0	30.0	39.0
82-83	34.51200030310268	35.0	34.0	37.0	30.0	39.0
84-85	34.27514305611459	35.0	34.0	36.0	30.0	37.5
86-87	34.054683311036854	35.0	34.0	36.0	30.0	37.0
88-89	33.84619089843304	35.0	34.0	35.5	30.0	37.0
90-91	33.66637318722569	35.0	34.0	35.0	30.0	36.0
92-93	33.564141688048345	35.0	34.0	35.0	29.5	36.0
94-95	33.53012687707829	35.0	34.0	35.0	29.5	36.0
96-97	33.479519884125885	35.0	33.5	35.0	29.0	36.0
98-99	33.50947931003016	35.0	33.0	35.0	30.0	35.5
100	32.89751794859445	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	7.0
16	42.0
17	195.0
18	966.0
19	2626.0
20	5841.0
21	11106.0
22	17432.0
23	24601.0
24	32070.0
25	41620.0
26	48969.0
27	53626.0
28	62831.0
29	76854.0
30	91039.0
31	110209.0
32	144560.0
33	196326.0
34	280188.0
35	417729.0
36	684350.0
37	1244852.0
38	1893565.0
39	1203894.0
40	78197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.729616459516244	20.99377767915479	14.592225080933897	23.68438078039507
2	14.174315707044455	21.542668955998593	41.779663008833126	22.503352328123825
3	17.531078099506146	26.729850460097033	30.8543095227435	24.88476191765332
4	12.193979229727283	18.848420856301143	39.7315984465503	29.22600146742127
5	13.148439408087963	38.54486060294873	35.695979756734545	12.610720232228761
6	28.86958229028616	38.60741117551602	19.65936793944451	12.863638594753304
7	25.820250113854428	33.384303825181476	23.701115104072315	17.094330956891785
8	23.942308636242345	36.83641708584421	21.86927253347644	17.352001744437004
9	25.037834769852303	17.830852140901367	21.86431005071458	35.26700303853175
10-11	23.437524285371456	27.504311222803267	29.534216893865572	19.523947597959708
12-13	24.159069417727903	25.17066339535951	29.283407474450968	21.38685971246162
14-15	21.62830064106406	26.67337264605919	26.735625810514485	24.962700902362258
16-17	21.463347450127422	29.55807255637521	27.452176967900627	21.526403025596743
18-19	21.53137919794673	29.219539450047943	28.460146580605166	20.78893477140016
20-21	22.52000936242789	28.33319619033108	28.11108404624135	21.03571040099969
22-23	22.295992880891546	28.193840404782033	27.842091349036952	21.668075365289464
24-25	22.011969168922775	28.33729424964352	27.95525828402676	21.695478297406954
26-27	21.920926672737554	28.47923065031261	27.85439802418476	21.745444652765077
28-29	21.86630129589552	28.56375001698583	27.873849126122053	21.696099560996597
30-31	22.21848788777807	28.334893849611014	27.790409934922096	21.65620832768882
32-33	21.943517523235865	28.393249126920722	27.815686296862857	21.84754705298056
34-35	22.122386247603902	28.46346437529296	27.814722431232862	21.59942694587027
36-37	22.103526502491665	28.266018801190363	27.83182073766656	21.798633958651408
38-39	21.874661639774253	28.304654072670953	28.034551369317224	21.786132918237573
40-41	22.097572184839283	28.165973766382812	27.985792400489146	21.750661648288762
42-43	22.002804799110862	28.186045466435417	27.874790184542174	21.93635954991155
44-45	21.995399006741426	28.20966850086072	27.89748646745041	21.897446024947445
46-47	21.953039922146075	28.150915422830437	27.868594262589042	22.027450392434453
48-49	21.814827416483055	28.134474027253898	28.052952070122885	21.997746486140166
50-51	21.726234425026366	28.096931261176337	28.06060285714444	22.11623145665286
52-53	21.85858464518824	27.920687122802423	28.018222440428893	22.20250579158044
54-55	21.85376193728074	27.725021238806725	28.186541203285042	22.234675620627495
56-57	21.75338122106646	27.580619305238162	28.266786371428477	22.399213102266902
58-59	21.585635635332814	27.56724757738037	28.44104176067311	22.40607502661371
60-61	21.476351791073725	27.397591633659324	28.794435497866434	22.331621077400516
62-63	21.37513876521514	27.648459266810516	28.763992701231878	22.212409266742466
64-65	21.22066229336268	27.958623880231258	28.414403582613385	22.406310243792678
66-67	21.06713720026249	28.16569893390819	28.166638149891522	22.6005257159378
68-69	21.230646713134206	28.332326938474182	28.11121728205359	22.325809066338014
70-71	21.24456414189456	28.446676029575706	27.758337792358255	22.550422036171476
72-73	21.623554762526137	28.2366370985046	27.368366074484967	22.771442064484297
74-75	21.87326438188985	28.049431171490134	27.382953817193346	22.694350629426665
76-77	22.09739766598039	27.5909061965979	27.47642089383954	22.83527524358217
78-79	22.463285579963145	27.363769121256954	27.30004324069068	22.872902058089224
80-81	22.85702388158906	27.305205348458873	27.004538210091606	22.83323255986046
82-83	22.518318411133567	27.66454785099014	26.874876105958574	22.94225763191772
84-85	23.003537109726576	27.63127002688312	26.830625448599914	22.534567414790388
86-87	22.86776370361283	27.942654002832583	26.871318746075378	22.31826354747921
88-89	22.76325655932517	28.178853457448298	26.747012283716643	22.310877699509884
90-91	22.915637238330593	28.315874703417837	27.184607128376488	21.58388092987508
92-93	23.08206083027237	28.048033028978402	27.46385718906946	21.40604895167976
94-95	22.73456553913957	28.05097671475862	27.92055814236796	21.293899603733852
96-97	23.02059760802426	27.733483236954964	28.204152127456233	21.04176702756454
98-99	23.7244482368185	29.764796137161316	28.71644581804642	17.79430980797376
100	22.86638428100492	31.018672466286468	22.56826764454975	23.546675608158864
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2631.0
1	2035.5
2	1592.5
3	1690.0
4	1649.5
5	1793.5
6	2148.0
7	2555.0
8	2851.5
9	3158.5
10	3464.5
11	3670.5
12	3972.0
13	4425.0
14	5078.5
15	6065.5
16	7370.0
17	8833.0
18	10511.5
19	12484.0
20	14938.5
21	18297.5
22	23047.0
23	29428.5
24	37681.0
25	48239.5
26	61508.0
27	76529.5
28	92559.5
29	110059.5
30	126156.5
31	141241.5
32	157303.5
33	172542.0
34	187518.0
35	201970.0
36	217609.0
37	232669.5
38	243396.5
39	251528.0
40	259381.5
41	266230.0
42	270980.5
43	275400.0
44	279048.0
45	282201.5
46	282903.5
47	282158.5
48	281001.5
49	275813.5
50	267856.5
51	257928.5
52	244903.5
53	229590.0
54	214543.0
55	198128.0
56	179089.5
57	159041.5
58	139687.0
59	121028.5
60	101879.0
61	83443.5
62	66319.0
63	51820.0
64	40059.0
65	30494.5
66	23497.5
67	18635.5
68	14641.5
69	11191.0
70	8707.5
71	6881.5
72	5416.5
73	4252.0
74	3317.5
75	2580.5
76	1963.0
77	1503.5
78	1175.0
79	930.0
80	729.0
81	555.0
82	439.5
83	303.0
84	206.0
85	161.0
86	138.0
87	120.0
88	92.5
89	68.0
90	52.0
91	41.5
92	31.5
93	20.0
94	14.5
95	14.5
96	14.0
97	11.0
98	5.5
99	6.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.022353773526677362
3	0.013028546646287005
4	0.005026996308727178
5	0.009979628766733539
6	0.009027771477507092
7	0.0029596812586884865
8	0.009087262558083745
9	0.005711143735358687
10-11	0.0038148655419778734
12-13	0.008841861850705051
14-15	0.005116232929592158
16-17	0.003911538547914934
18-19	0.005435997487691667
20-21	0.004582796627835401
22-23	0.011113996101689566
24-25	0.009929862986513934
26-27	0.023403558921572355
28-29	0.020326597588084597
30-31	0.01636422884282091
32-33	0.01325080886551854
34-35	0.009307726376019806
36-37	0.010883245674079896
38-39	0.00548189548891703
40-41	0.006748598116467795
42-43	0.004371004388319106
44-45	0.007925172734678852
46-47	0.010465072177782302
48-49	0.007825180897341412
50-51	0.010846778704226278
52-53	0.0010300967324222312
54-55	0.0030632146723856197
56-57	7.232463620310602E-4
58-59	0.003041019209529249
60-61	0.0011324878381174033
62-63	0.00738852414110208
64-65	0.003960193108714413
66-67	0.00427937490746056
68-69	0.005382560536882345
70-71	8.763566000168261E-4
72-73	5.653213649456541E-4
74-75	0.0
76-77	6.039615911488911E-5
78-79	4.191325632471038E-4
80-81	2.0334421666945158E-4
82-83	0.0
84-85	9.703039826353673E-4
86-87	0.0034695156574548114
88-89	0.022032532431616656
90-91	0.003104208566362568
92-93	0.0012927705665084631
94-95	0.0010806695238913928
96-97	4.2318911082023014E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2016.0
20-21	3787.0
22-23	4587.0
24-25	5460.0
26-27	7377.0
28-29	10907.0
30-31	17215.0
32-33	20950.0
34-35	27147.0
36-37	37458.0
38-39	41809.0
40-41	38537.0
42-43	40592.0
44-45	46354.0
46-47	49756.0
48-49	28759.0
50-51	28465.0
52-53	9605.0
54-55	9413.0
56-57	9946.0
58-59	11241.0
60-61	11835.0
62-63	58390.0
64-65	63384.0
66-67	65498.0
68-69	66025.0
70-71	65482.0
72-73	63951.0
74-75	65825.0
76-77	68365.0
78-79	70122.0
80-81	71443.0
82-83	70217.0
84-85	73686.0
86-87	89110.0
88-89	81308.0
90-91	83474.0
92-93	89922.0
94-95	110270.0
96-97	244496.0
98-99	441069.0
100-101	4318444.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.36344119599318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.2149802618429	77.91276142841353
2	6.970453437021859	12.039846910352917
3	1.6794912344152004	4.351399273878094
4	0.5488099531028109	1.8958846445028155
5	0.2442581577313636	1.0547487520937124
6	0.12705945616101386	0.6583975122333956
7	0.06836723025021196	0.41331004886129746
8	0.039071235795724364	0.2699461100079059
9	0.02451682852928014	0.190562191110066
>10	0.08125154409472207	1.0228035721754907
>50	0.00127096730664916	0.0706810594204327
>100	4.194555238999674E-4	0.0802409903083359
>500	2.511911286200594E-5	0.016422153811008977
>1k	2.5119111496084414E-5	0.022995352831029433
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4872770144163248E-5	0.0
2	0.0	2.9745540288326496E-5	0.0	1.4872770144163248E-5	0.0
3	0.0	2.9745540288326496E-5	0.0	4.4618310432489744E-5	0.0
4	0.0	5.949108057665299E-5	0.0	4.4618310432489744E-5	0.0
5	0.0	5.949108057665299E-5	0.0	4.4618310432489744E-5	0.0
6	5.949108057665299E-5	5.949108057665299E-5	0.0	4.4618310432489744E-5	0.0
7	5.949108057665299E-5	5.949108057665299E-5	0.0	4.4618310432489744E-5	0.0
8	5.949108057665299E-5	7.436385072081624E-5	0.0	4.4618310432489744E-5	0.0
9	5.949108057665299E-5	1.0410939100914274E-4	0.0	5.949108057665299E-5	0.0
10-11	5.949108057665299E-5	1.0410939100914274E-4	0.0	6.692746564873462E-5	0.0
12-13	5.949108057665299E-5	1.0410939100914274E-4	0.0	8.923662086497949E-5	0.0
14-15	5.949108057665299E-5	1.7847324172995898E-4	0.0	8.923662086497949E-5	0.0
16-17	8.923662086497949E-5	1.7847324172995898E-4	0.0	1.4129131636955088E-4	1.4872770144163248E-5
18-19	8.923662086497949E-5	1.9334601187412224E-4	0.0	1.4872770144163248E-4	1.4872770144163248E-5
20-21	8.923662086497949E-5	2.0821878201828547E-4	0.0	1.7103685665787735E-4	1.4872770144163248E-5
22-23	8.923662086497949E-5	2.0821878201828547E-4	0.0	2.677098625949385E-4	1.4872770144163248E-5
24-25	8.923662086497949E-5	2.3796432230661197E-4	0.0	4.908014147573872E-4	1.4872770144163248E-5
26-27	8.923662086497949E-5	2.5283709245077523E-4	0.0	0.0011154577608122435	1.4872770144163248E-5
28-29	8.923662086497949E-5	2.5283709245077523E-4	0.0	0.00435028526716775	1.4872770144163248E-5
30-31	8.923662086497949E-5	2.825826327391017E-4	0.0	0.014701733287505372	1.4872770144163248E-5
32-33	1.0410939100914274E-4	2.825826327391017E-4	0.0	0.04948170626963112	1.4872770144163248E-5
34-35	1.1898216115330598E-4	3.3463732824367306E-4	0.0	0.09705226157573728	2.9745540288326496E-5
36-37	1.1898216115330598E-4	3.866920237482445E-4	0.0	0.15921300439326758	2.9745540288326496E-5
38-39	1.1898216115330598E-4	3.866920237482445E-4	0.0	0.2289662963693932	2.9745540288326496E-5
40-41	1.1898216115330598E-4	4.1643756403657095E-4	0.0	0.29787184044730153	2.9745540288326496E-5
42-43	1.3385493129746925E-4	4.313103341807342E-4	0.0	0.42404498596531043	2.9745540288326496E-5
44-45	1.4872770144163248E-4	4.610558744690607E-4	0.0	0.5533414132135936	2.9745540288326496E-5
46-47	1.7847324172995898E-4	4.7592864461322394E-4	0.0	0.6983509221191853	2.9745540288326496E-5
48-49	1.7847324172995898E-4	4.7592864461322394E-4	0.0	0.8024008220477514	4.4618310432489744E-5
50-51	1.859096268020406E-4	5.800380356223667E-4	0.0	1.1919632904338193	4.4618310432489744E-5
52-53	1.9334601187412224E-4	5.800380356223667E-4	0.0	1.7155219814337261	5.205469550457137E-5
54-55	1.9334601187412224E-4	5.874744206944483E-4	0.0	2.4128838643383244	5.949108057665299E-5
56-57	1.9334601187412224E-4	6.097835759106932E-4	0.0	3.1671340930443472	5.949108057665299E-5
58-59	1.9334601187412224E-4	6.097835759106932E-4	0.0	3.931103677039581	5.949108057665299E-5
60-61	2.3052793723453034E-4	6.172199609827748E-4	0.0	4.677077209160377	5.949108057665299E-5
62-63	2.3796432230661197E-4	6.246563460548564E-4	0.0	5.227540741351075	5.949108057665299E-5
64-65	2.3796432230661197E-4	7.436385072081624E-4	0.0	5.246258122577505	5.949108057665299E-5
66-67	2.3796432230661197E-4	7.436385072081624E-4	0.0	5.254765347099966	5.949108057665299E-5
68-69	2.3796432230661197E-4	7.585112773523257E-4	0.0	5.262685097201732	5.949108057665299E-5
70-71	2.3796432230661197E-4	7.808204325685706E-4	0.0	5.269705044709778	5.949108057665299E-5
72-73	2.825826327391017E-4	7.882568176406521E-4	0.0	5.276434973200011	7.436385072081624E-5
74-75	2.825826327391017E-4	8.254387430010603E-4	0.0	5.282562554499407	7.436385072081624E-5
76-77	2.825826327391017E-4	8.774934385056317E-4	0.0	5.286964894462079	7.436385072081624E-5
78-79	2.825826327391017E-4	8.923662086497949E-4	0.0	5.289485829001515	7.436385072081624E-5
80-81	2.825826327391017E-4	8.998025937218765E-4	0.0	5.290623595917544	7.436385072081624E-5
82-83	2.825826327391017E-4	9.072389787939581E-4	0.0	5.291508525741121	7.436385072081624E-5
84-85	2.9001901781118333E-4	9.072389787939581E-4	0.0	5.291865472224581	7.436385072081624E-5
86-87	2.9745540288326496E-4	9.221117489381214E-4	0.0	5.291999327155878	7.436385072081624E-5
88	2.9745540288326496E-4	9.964755996589377E-4	0.0	5.292014199926022	7.436385072081624E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5390	0.0	40.442116	1
GTATCAA	9220	0.0	32.409218	1
TCAACGC	10035	0.0	28.570623	4
ATCAACG	10220	0.0	28.01347	3
CAACGCA	10305	0.0	27.738005	5
AACGCAG	10790	0.0	26.732412	6
TATCAAC	11360	0.0	25.320116	2
ACGCAGA	12785	0.0	22.395247	7
GTGTAGA	11475	0.0	22.036493	94
TGTAGAT	10985	0.0	21.92039	94
CGCAGAG	13180	0.0	21.65867	8
GGTGTAG	13185	0.0	21.162504	94
GTACATG	21030	0.0	19.585781	1
TACATGG	20835	0.0	19.02634	2
GGACCGA	1065	0.0	18.567154	6
ACATGGG	21315	0.0	18.090506	3
GCAGAGT	16335	0.0	17.238316	9
AGGTGTA	15885	0.0	16.720984	94
CATGGGG	15700	0.0	15.989117	4
AGAGTAC	14350	0.0	15.743276	10-11
>>END_MODULE