FastQCFastQC Report
Fri 27 May 2016
SRR2140339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7960572
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66850.038.2071081
GTATCAA113200.030.8718341
TCAACGC119550.027.3138434
ATCAACG119900.027.1995053
CAACGCA122200.026.6504235
AACGCAG127700.025.7062366
TATCAAC135800.024.781462
ACGCAGA150500.021.6984467
CGCAGAG153850.021.2258428
GTACATG251250.020.5375651
TACATGG244950.020.2207152
ACATGGG249950.019.2088073
GTGTAGA132100.017.90085694
CATGGGG181250.016.6354924
GGACCGA12200.016.6181556
GCAGAGT194950.016.5517889
TGTAGAT127500.016.47413894
ACCGAAC10650.016.2023098
ACTATCG8300.015.85205610-11
AGAGTAC170950.015.30472510-11
Produced by FastQC (version 0.11.5)