##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140339_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7960572
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.875415485218902	33.0	31.0	34.0	30.0	34.0
2	32.02454082445332	34.0	31.0	34.0	30.0	34.0
3	32.168782846257784	34.0	31.0	34.0	30.0	34.0
4	35.61665895365308	37.0	35.0	37.0	35.0	37.0
5	35.725196505979724	37.0	35.0	37.0	35.0	37.0
6	35.83727890407875	37.0	36.0	37.0	35.0	37.0
7	35.78528578097152	37.0	36.0	37.0	35.0	37.0
8	35.73187994531046	37.0	36.0	37.0	35.0	37.0
9	37.48477257664399	39.0	38.0	39.0	35.0	39.0
10-11	37.468720589424976	39.0	38.0	39.0	35.0	39.0
12-13	37.397141248141466	39.0	38.0	39.0	35.0	39.0
14-15	38.819529802632275	41.0	39.0	41.0	35.0	41.0
16-17	38.83363821092253	41.0	39.0	41.0	35.0	41.0
18-19	38.85296439753324	41.0	39.0	41.0	35.0	41.0
20-21	38.889770540033766	41.0	39.0	41.0	35.0	41.0
22-23	38.86412892655504	40.0	39.0	41.0	34.0	41.0
24-25	38.82731126876847	40.0	38.5	41.0	34.0	41.0
26-27	38.77162920029966	40.0	38.0	41.0	34.0	41.0
28-29	38.699096245262936	40.0	38.0	41.0	34.0	41.0
30-31	38.57620753245111	40.0	38.0	41.0	34.0	41.0
32-33	38.51163701321656	40.0	38.0	41.0	34.0	41.0
34-35	38.35551548206324	40.0	38.0	41.0	33.0	41.0
36-37	38.247063147007964	40.0	38.0	41.0	33.0	41.0
38-39	38.23578143164352	40.0	38.0	41.0	33.0	41.0
40-41	38.14840613410833	40.0	38.0	41.0	33.0	41.0
42-43	37.975233166507145	40.0	38.0	41.0	33.0	41.0
44-45	38.043629220471	40.0	37.5	41.0	33.0	41.0
46-47	38.24850284699946	40.0	38.0	41.0	33.0	41.0
48-49	38.238509375862165	40.0	38.0	41.0	33.0	41.0
50-51	38.14899128157035	40.0	38.0	41.0	33.0	41.0
52-53	38.07392808553551	40.0	37.0	41.0	33.0	41.0
54-55	37.993881361658715	40.0	37.0	41.0	33.0	41.0
56-57	38.03824074748871	40.0	37.0	41.0	33.0	41.0
58-59	37.853062991526485	40.0	36.5	41.0	33.0	41.0
60-61	37.77137439869169	40.0	36.0	41.0	33.0	41.0
62-63	37.54149163625908	39.0	36.0	41.0	32.0	41.0
64-65	37.33540958696703	39.0	35.0	41.0	32.0	41.0
66-67	37.057289071719566	39.0	35.0	41.0	32.0	41.0
68-69	36.818212620042914	38.0	35.0	40.0	31.5	41.0
70-71	36.52132815561505	37.0	35.0	40.0	31.0	41.0
72-73	36.183160026101405	37.0	35.0	39.5	31.0	41.0
74-75	35.835718330147074	36.0	35.0	39.0	31.0	41.0
76-77	35.496502619856074	36.0	34.0	39.0	31.0	40.5
78-79	35.121117315568235	35.0	34.0	37.5	30.5	39.5
80-81	34.798395972783595	35.0	34.0	37.0	30.0	39.0
82-83	34.472312468667155	35.0	34.0	36.5	30.0	39.0
84-85	34.24474412419451	35.0	34.0	36.0	30.0	37.5
86-87	33.99207085490882	35.0	34.0	36.0	30.0	37.0
88-89	33.73520242969653	35.0	34.0	35.5	29.5	37.0
90-91	33.54368368228905	35.0	33.5	35.0	29.0	36.0
92-93	33.44360704555992	35.0	33.0	35.0	29.0	36.0
94-95	33.41817482025763	35.0	33.0	35.0	29.0	36.0
96-97	33.385284499071545	35.0	33.0	35.0	29.0	36.0
98-99	33.432295510417575	35.0	33.0	35.0	29.0	35.5
100	32.83619601979613	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	5.0
16	53.0
17	273.0
18	1156.0
19	3317.0
20	7497.0
21	13660.0
22	21028.0
23	29713.0
24	39831.0
25	50416.0
26	59514.0
27	64858.0
28	75182.0
29	92582.0
30	108626.0
31	131944.0
32	173367.0
33	236767.0
34	341288.0
35	511238.0
36	837811.0
37	1508390.0
38	2206914.0
39	1359075.0
40	86065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.71753889042144	20.851453890499325	14.596513918849046	23.834493300230182
2	14.235674575961117	21.428133459951738	41.66110261041144	22.675089353675705
3	17.54437145000132	26.613987544459395	30.689565061165823	25.152075944373458
4	12.22789699975189	18.805453397695402	39.56391662243606	29.402732980116642
5	13.294289349364508	38.30100753278279	35.610015451507785	12.794687666344922
6	29.08299318702331	38.42106783955899	19.558356292424065	12.937582680993643
7	25.969322617023145	33.19548304987974	23.637873167773133	17.197321165323988
8	24.008370034312314	36.740334962180064	21.842153796365345	17.409141207142266
9	25.084995652699902	17.761092084999298	21.75408816432631	35.39982409797449
10-11	23.58232473223331	27.36346001562892	29.44842816640676	19.605787085731006
12-13	24.26624726093534	25.025201530584685	29.18319025246004	21.525360956019938
14-15	21.731432871408526	26.486161727701795	26.649663260960505	25.13274213992917
16-17	21.559427707135196	29.403668975636478	27.372680038089207	21.664223279139126
18-19	21.634543662769293	29.0892124482521	28.38442279439638	20.891821094582227
20-21	22.596923998870594	28.15519050917679	28.072672049041262	21.175213442911346
22-23	22.36853383613028	28.04118039808825	27.796259322027822	21.794026443753648
24-25	22.058498227684	28.215421745129476	27.870392017734176	21.85568800945235
26-27	22.026155060243976	28.36702885730001	27.77607651921532	21.830739563240694
28-29	21.980549415349127	28.417369387355713	27.801748916631713	21.800332280663447
30-31	22.251466626789657	28.205877575682454	27.75524587695908	21.787409920568813
32-33	22.045988588899505	28.2487800232801	27.74720167681583	21.95802971100456
34-35	22.208550597877487	28.26538705342041	27.78329087087654	21.742771477825556
36-37	22.196915760962202	28.08473446896565	27.81908140880361	21.899268361268536
38-39	21.925299036776657	28.101269130313128	28.012089492678978	21.961342340231237
40-41	22.149778773343883	27.936616254218155	28.00193900968307	21.911665962754885
42-43	22.015215593275304	28.007038866658874	27.894643976844492	22.083101563221334
44-45	22.034797007909464	28.045756622678493	27.90791383048929	22.011532538922758
46-47	21.967105051619907	27.976776461655906	27.87045944993209	22.185659036792103
48-49	21.801033357738596	27.974145149043046	28.03538365478657	22.189437838431783
50-51	21.67318167946031	27.984265927787	28.051848324222206	22.290704068530484
52-53	21.796805312706	27.81562163366666	28.039624189140117	22.347948864487226
54-55	21.863558366870166	27.6223351326717	28.105469197133765	22.408637303324365
56-57	21.721111152295318	27.506973834487663	28.159545013465014	22.612369999752005
58-59	21.67181812680807	27.480678851977103	28.28890975387201	22.558593267342815
60-61	21.584539553660502	27.274408842120167	28.61638395767955	22.524667646539783
62-63	21.494961074082315	27.480526292595208	28.55284987375814	22.47166275956434
64-65	21.372399495196976	27.74942617065898	28.271281520440787	22.60689281370326
66-67	21.186876291052346	28.018942421428527	28.000502241454566	22.793679046064554
68-69	21.276292573065582	28.12559284561186	27.995800333613698	22.60231424770886
70-71	21.288173259630273	28.190111789911704	27.69671123924545	22.82500371121258
72-73	21.592265968933123	28.047388522773748	27.333114937828036	23.0272305704651
74-75	21.868772769986368	27.859883104054905	27.36088203616186	22.91046208979687
76-77	22.013458820069193	27.410390086351015	27.499638865223343	23.076512228356446
78-79	22.315958466798975	27.16881866466091	27.4448054005911	23.07041746794901
80-81	22.67141117325958	27.220233344153417	27.04350200762909	23.064853474957914
82-83	22.363720349260667	27.242338043532794	27.2468286280858	23.147112979120738
84-85	22.52857097431043	27.390381564750925	27.167610663507887	22.913436797430762
86-87	22.231930461360932	27.71529180131587	27.331834000420795	22.7209437369024
88-89	21.994281083929	27.79615114600147	27.377693295919585	22.831874474149945
90-91	21.870242586046093	27.967071884825312	27.67802944108098	22.484656088047615
92-93	21.801549304811978	27.563595764062725	28.204007046578695	22.4308478845466
94-95	21.46388171261505	27.535807514128212	28.636325619206154	22.363985154050585
96-97	21.898349632223205	27.423966727319986	28.53111125936783	22.146572381088976
98-99	23.320249020170156	29.286820915184975	28.92447244527292	18.468457619371954
100	22.449297272353558	30.585695595376162	22.646168081366945	24.318839050903332
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3028.0
1	2354.5
2	1870.5
3	2011.5
4	1973.5
5	2156.0
6	2558.5
7	2982.0
8	3293.0
9	3646.0
10	4013.0
11	4273.5
12	4622.5
13	5172.5
14	5943.5
15	7052.0
16	8422.5
17	10010.5
18	11970.5
19	14229.0
20	16967.0
21	20476.5
22	25565.5
23	32516.0
24	41709.0
25	53649.0
26	68514.5
27	85869.5
28	104248.0
29	123648.0
30	142072.0
31	160138.0
32	179123.5
33	197275.5
34	215288.5
35	232979.5
36	251846.0
37	269894.5
38	282168.0
39	291871.5
40	303066.5
41	313382.5
42	321140.5
43	327328.0
44	332179.0
45	336938.0
46	338295.0
47	336220.0
48	333579.0
49	327252.5
50	318234.0
51	306166.0
52	291367.5
53	275191.5
54	257581.5
55	236439.5
56	213823.5
57	191173.0
58	167787.0
59	145348.0
60	121615.0
61	98863.0
62	79228.0
63	61860.5
64	46992.0
65	35945.5
66	27843.5
67	21886.5
68	17414.5
69	13530.5
70	10490.0
71	8268.0
72	6615.0
73	5220.0
74	4080.0
75	3166.5
76	2383.5
77	1816.5
78	1414.5
79	1094.5
80	846.0
81	663.0
82	516.0
83	360.0
84	260.0
85	206.5
86	179.0
87	152.5
88	108.0
89	77.0
90	56.0
91	42.5
92	33.5
93	21.0
94	13.0
95	13.0
96	15.0
97	13.5
98	6.0
99	6.5
100	25.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.021983344915415627
3	0.013340749885812226
4	0.0049870788179542875
5	0.010438948356977363
6	0.009572176471740976
7	0.0030776682881581874
8	0.009396309712417651
9	0.005765917323529012
10-11	0.0041517117111684945
12-13	0.009270690598615275
14-15	0.005049888374855475
16-17	0.003919316350634101
18-19	0.005860131658880794
20-21	0.004706680193083229
22-23	0.01065198244145827
24-25	0.009923355450816717
26-27	0.024128179774148648
28-29	0.019931857135812938
30-31	0.015665319049173246
32-33	0.012895848581339535
34-35	0.009497279054944553
36-37	0.010767698001878422
38-39	0.005865978904121151
40-41	0.0066587036985042
42-43	0.004134325329866107
44-45	0.007671805496630653
46-47	0.010031495096779372
48-49	0.007669889031280484
50-51	0.010491990219928408
52-53	8.836183600481725E-4
54-55	0.0029414297451484045
56-57	6.933120717631326E-4
58-59	0.00285850112222884
60-61	0.001030589433580686
62-63	0.007321921793545719
64-65	0.0037289973546492765
66-67	0.0037879023987011807
68-69	0.005441766932030115
70-71	7.628837060078561E-4
72-73	6.863137782726784E-4
74-75	0.0
76-77	1.447433765069052E-5
78-79	4.762791024850045E-4
80-81	1.8557164156525672E-4
82-83	0.0
84-85	9.989284776360503E-4
86-87	0.0035400810376450048
88-89	0.02191768043817083
90-91	0.0028363398268422546
92-93	0.0013533996298044362
94-95	0.0011971860142734503
96-97	4.2711420034269933E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2610.0
20-21	4935.0
22-23	5615.0
24-25	6660.0
26-27	8504.0
28-29	12088.0
30-31	17498.0
32-33	20403.0
34-35	26344.0
36-37	37496.0
38-39	43367.0
40-41	38823.0
42-43	40320.0
44-45	46139.0
46-47	54640.0
48-49	33010.0
50-51	33011.0
52-53	12583.0
54-55	12869.0
56-57	13659.0
58-59	14694.0
60-61	15129.0
62-63	67533.0
64-65	73750.0
66-67	77374.0
68-69	78103.0
70-71	77465.0
72-73	76945.0
74-75	79253.0
76-77	84436.0
78-79	86967.0
80-81	88855.0
82-83	89640.0
84-85	93945.0
86-87	113431.0
88-89	105370.0
90-91	106746.0
92-93	114583.0
94-95	140708.0
96-97	299362.0
98-99	543506.0
100-101	5062203.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.85478266331273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41977786910051	75.87695816884225
2	7.31188954737642	12.408975968015485
3	1.8719896262735336	4.765418186562502
4	0.6544987098340703	2.221493831055545
5	0.30155062581557135	1.2794006407783123
6	0.15694158657803578	0.7990346531948822
7	0.08641460456667531	0.5132884742608942
8	0.05475211922074603	0.3716783341465753
9	0.033411879803168926	0.2551642019163564
>10	0.10692736850575965	1.3027143277549633
>50	0.0013758878024590483	0.07927667023371833
>100	4.0197885633911685E-4	0.07710032536070506
>500	6.819626677550655E-5	0.04949621787781758
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.79333796616625E-5	0.0	0.0	0.0
3	0.0	8.79333796616625E-5	0.0	0.0	0.0
4	0.0	1.381810251826125E-4	0.0	0.0	1.25619113802375E-5
5	0.0	1.381810251826125E-4	0.0	1.25619113802375E-5	1.25619113802375E-5
6	1.25619113802375E-5	1.381810251826125E-4	0.0	1.25619113802375E-5	1.25619113802375E-5
7	1.25619113802375E-5	1.381810251826125E-4	0.0	3.76857341407125E-5	1.25619113802375E-5
8	1.25619113802375E-5	1.381810251826125E-4	0.0	3.76857341407125E-5	1.25619113802375E-5
9	1.25619113802375E-5	2.5123822760475E-4	0.0	7.5371468281425E-5	1.25619113802375E-5
10-11	3.140477845059375E-5	2.5123822760475E-4	0.0	1.130572024221375E-4	1.25619113802375E-5
12-13	3.76857341407125E-5	2.5123822760475E-4	0.0	1.75866759323325E-4	1.884286707035625E-5
14-15	3.76857341407125E-5	3.642954300268875E-4	0.0	1.8842867070356252E-4	2.5123822760475E-5
16-17	5.024764552095E-5	3.7685734140712504E-4	0.0	2.1983344915415626E-4	2.5123822760475E-5
18-19	5.024764552095E-5	3.8941925278736255E-4	0.0	2.5123822760475E-4	3.140477845059375E-5
20-21	5.024764552095E-5	4.1454307554783754E-4	0.0	4.019811641676E-4	3.76857341407125E-5
22-23	5.024764552095E-5	5.27600277969975E-4	0.0	7.223099043636562E-4	4.396668983083125E-5
24-25	6.28095569011875E-5	6.155336576316375E-4	0.0	0.0013755292961360062	5.6528601211068754E-5
26-27	7.5371468281425E-5	6.5321939177235E-4	0.0	0.002838991971933675	6.28095569011875E-5
28-29	8.79333796616625E-5	6.657813031525875E-4	0.0	0.010570848426469857	6.28095569011875E-5
30-31	8.79333796616625E-5	7.662765941944875E-4	0.0	0.036768714609955164	6.909051259130625E-5
32-33	8.79333796616625E-5	7.662765941944875E-4	0.0	0.11518016544539764	7.5371468281425E-5
34-35	9.421433535178125E-5	8.918957079968625E-4	0.0	0.21668669035340676	7.5371468281425E-5
36-37	1.004952910419E-4	0.0010175148217992376	0.0	0.35978570384138225	7.5371468281425E-5
38-39	1.004952910419E-4	0.001030076733179475	0.0	0.5186373542001755	7.5371468281425E-5
40-41	1.004952910419E-4	0.0011682577583620876	0.0	0.6615416580617574	7.5371468281425E-5
42-43	1.1305720242213751E-4	0.0011933815811225627	0.0	0.8912550505164705	7.5371468281425E-5
44-45	1.1305720242213751E-4	0.0013001578278545815	0.0	1.1062710066563057	7.5371468281425E-5
46-47	1.25619113802375E-4	0.0013692483404458876	0.0	1.3489043249655928	7.5371468281425E-5
48-49	1.25619113802375E-4	0.0013755292961360062	0.0	1.5040439305115259	1.004952910419E-4
50-51	1.25619113802375E-4	0.001532553188388975	0.0	1.9073880620638817	1.004952910419E-4
52-53	1.25619113802375E-4	0.0015451150997692125	0.0	2.425937482884396	1.0677624673201876E-4
54-55	1.25619113802375E-4	0.0015513960554593312	0.0	3.094457282717875	1.1305720242213751E-4
56-57	1.3190006949249374E-4	0.0015828008339099251	0.0	3.8161956201137306	1.1305720242213751E-4
58-59	1.381810251826125E-4	0.0015828008339099251	0.0	4.560256474032268	1.25619113802375E-4
60-61	1.381810251826125E-4	0.0016393294351209938	0.0	5.282710840376797	1.381810251826125E-4
62-63	1.5074293656285E-4	0.0016770151692617062	0.0	5.815775549797175	1.381810251826125E-4
64-65	1.5074293656285E-4	0.001934534352556575	0.0	5.843053740359361	1.381810251826125E-4
66-67	1.5074293656285E-4	0.0019408153082466938	0.0	5.8520606308189915	1.4446198087273126E-4
68-69	1.5074293656285E-4	0.002009905820838	0.0	5.860093973146654	1.5074293656285E-4
70-71	1.5074293656285E-4	0.0020413105992885937	0.0	5.8670030244057845	1.5074293656285E-4
72-73	1.5074293656285E-4	0.0020727153777391873	0.0	5.873478689722296	1.75866759323325E-4
74-75	1.5074293656285E-4	0.002173210668781088	0.0	5.87950840718481	1.75866759323325E-4
76-77	1.5702389225296875E-4	0.0023113916939637	0.0	5.883955323813415	1.75866759323325E-4
78-79	1.75866759323325E-4	0.00236163933948465	0.0	5.8863420869756595	1.75866759323325E-4
80-81	1.8214771501344376E-4	0.00236163933948465	0.0	5.887541749512472	1.884286707035625E-4
82-83	1.8842867070356252E-4	0.002367920295174769	0.0	5.888421083309089	2.3239536053439378E-4
84-85	1.8842867070356252E-4	0.0023930441179352436	0.0	5.888684883448073	2.449572719146313E-4
86-87	1.8842867070356252E-4	0.002468415586216669	0.0	5.888791659694806	2.5123822760475E-4
88	1.8842867070356252E-4	0.0026254394784696374	0.0	5.888823064473256	2.5123822760475E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6685	0.0	38.207108	1
GTATCAA	11320	0.0	30.871834	1
TCAACGC	11955	0.0	27.313843	4
ATCAACG	11990	0.0	27.199505	3
CAACGCA	12220	0.0	26.650423	5
AACGCAG	12770	0.0	25.706236	6
TATCAAC	13580	0.0	24.78146	2
ACGCAGA	15050	0.0	21.698446	7
CGCAGAG	15385	0.0	21.225842	8
GTACATG	25125	0.0	20.537565	1
TACATGG	24495	0.0	20.220715	2
ACATGGG	24995	0.0	19.208807	3
GTGTAGA	13210	0.0	17.900856	94
CATGGGG	18125	0.0	16.635492	4
GGACCGA	1220	0.0	16.618155	6
GCAGAGT	19495	0.0	16.551788	9
TGTAGAT	12750	0.0	16.474138	94
ACCGAAC	1065	0.0	16.202309	8
ACTATCG	830	0.0	15.852056	10-11
AGAGTAC	17095	0.0	15.304725	10-11
>>END_MODULE