FastQCFastQC Report
Fri 27 May 2016
SRR2140338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8129610
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67450.038.2660561
GTATCAA111250.029.7578831
TCAACGC115400.027.092244
ATCAACG118550.026.4469343
CAACGCA119800.026.1674425
AACGCAG125950.025.1612436
TATCAAC130650.024.5564042
ACGCAGA149600.020.8998537
CGCAGAG151050.020.6993528
GTACATG238400.019.970931
TACATGG237450.019.3149092
ACATGGG240450.018.361373
TATACCG5650.018.1173025
CATGGGG176050.016.3536434
GCAGAGT194950.015.7979549
AGAGTAC163600.015.23996910-11
CAGAGTA188500.014.72037310-11
GTATAGC30700.014.0353531
GTGTAGC47200.014.0097381
TATACTG53200.013.7092995
Produced by FastQC (version 0.11.5)