FastQCFastQC Report
Fri 27 May 2016
SRR2140337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8228985
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77700.036.6907421
GTATCAA122250.029.411321
TCAACGC129000.026.2968044
ATCAACG130600.025.9429533
CAACGCA133550.025.3349045
AACGCAG138700.024.5220056
TCGGTGG56500.024.51297294
TATCAAC144400.024.1984272
ACGCAGA168100.020.2606477
GTACATG258500.020.1820091
CGCAGAG168700.020.057768
TACATGG255350.019.8964042
ACATGGG260650.018.8305823
CTCGGTG75250.018.75903594
TCTCGGT78700.016.62418492-93
CATGGGG191450.016.4532854
GCAGAGT210750.015.9725879
AGAGTAC178550.015.54603310-11
CAGAGTA206100.014.932322510-11
GTGGTAT53050.014.7014811
Produced by FastQC (version 0.11.5)