FastQCFastQC Report
Fri 27 May 2016
SRR2140336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7319581
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65100.037.6729431
GTATCAA106800.027.4385171
ATCAACG113650.024.7853583
TCAACGC113350.024.6638624
CAACGCA116900.023.9147115
AACGCAG121150.023.2146326
TATCAAC126500.022.5692562
ACGCAGA141150.019.8665647
CGCAGAG145350.019.3790198
GTACATG223750.019.3736841
TACATGG222750.018.783372
ACATGGG227700.018.0409073
CATGGGG171000.016.4468674
GCAGAGT178600.015.7483999
AGAGTAC152950.015.34726110-11
CAGAGTA174750.014.81194710-11
GTATAAG32550.013.3948251
TATACTG40800.013.1493295
GAGTACA171350.013.016141
GTGGTAT46900.012.8719491
Produced by FastQC (version 0.11.5)