FastQCFastQC Report
Fri 27 May 2016
SRR2140335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8547714
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70400.039.5876241
GTATCAA119700.030.5742761
TCAACGC127100.027.4960924
ATCAACG129000.027.092383
CAACGCA132950.026.22255
AACGCAG136350.025.5999876
TATCAAC144450.024.5477452
ACGCAGA161350.021.6088477
CGCAGAG168200.020.6032528
GTACATG265150.020.1790451
TACATGG259900.019.775642
ACATGGG262750.018.884963
ACCGAAC12600.017.3989378
GGACCGA15050.017.3667986
GCAGAGT210150.016.5711759
CATGGGG196750.016.1554344
CCGAACC13300.015.5327059
AGAGTAC178500.015.37557910-11
CGTACGA2757.2759576E-1215.01986760-61
CAGAGTA203650.015.00867310-11
Produced by FastQC (version 0.11.5)