FastQCFastQC Report
Fri 27 May 2016
SRR2140334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7779130
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87150.030.3516461
GTATCAA132650.026.292771
TCAACGC140500.023.6860084
ATCAACG141450.023.4395433
CAACGCA142150.023.4107725
AACGCAG147800.022.601066
TATCAAC156250.021.5710012
GTACATG248800.019.2076741
ACGCAGA174700.019.121857
TACATGG245950.018.9706672
CGCAGAG178700.018.6937088
ACATGGG256200.017.7838613
CATGGGG186500.015.8212784
GCAGAGT217000.015.3171479
AGAGTAC173450.015.09086710-11
GTACTAG19400.014.0453291
GGACCGA13750.014.0216796
CAGAGTA210400.013.87495110-11
GTATTAG26400.013.4969391
TATACTG45650.013.4964235
Produced by FastQC (version 0.11.5)