##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140334_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7779130
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.831445547252713	33.0	31.0	34.0	30.0	34.0
2	31.980304995545776	34.0	31.0	34.0	30.0	34.0
3	32.129889589195706	34.0	31.0	34.0	30.0	34.0
4	35.54864798505746	37.0	35.0	37.0	33.0	37.0
5	35.66513581852983	37.0	35.0	37.0	35.0	37.0
6	35.773999277554175	37.0	36.0	37.0	35.0	37.0
7	35.72234427243149	37.0	36.0	37.0	35.0	37.0
8	35.66727757474165	37.0	36.0	37.0	35.0	37.0
9	37.408013621060455	39.0	38.0	39.0	35.0	39.0
10-11	37.397073387383934	39.0	38.0	39.0	35.0	39.0
12-13	37.32476022382966	39.0	38.0	39.0	34.5	39.0
14-15	38.73923112224632	41.0	39.0	41.0	35.0	41.0
16-17	38.75457499746116	41.0	39.0	41.0	34.0	41.0
18-19	38.78264053949478	41.0	39.0	41.0	34.0	41.0
20-21	38.818977736884335	41.0	39.0	41.0	34.0	41.0
22-23	38.796344207634725	40.0	39.0	41.0	34.0	41.0
24-25	38.762120342905135	40.5	38.5	41.0	34.0	41.0
26-27	38.71159868876987	40.0	38.0	41.0	34.0	41.0
28-29	38.64460273139707	40.0	38.0	41.0	34.0	41.0
30-31	38.52580871231149	40.0	38.0	41.0	34.0	41.0
32-33	38.46436071551001	40.0	38.0	41.0	34.0	41.0
34-35	38.3132586474322	40.0	38.0	41.0	33.0	41.0
36-37	38.206866861550935	40.0	38.0	41.0	33.0	41.0
38-39	38.200628968578314	40.0	38.0	41.0	33.0	41.0
40-41	38.11713190799685	40.0	38.0	41.0	33.0	41.0
42-43	37.94617398454089	40.0	38.0	41.0	33.0	41.0
44-45	38.01795129497708	40.0	37.5	41.0	32.5	41.0
46-47	38.23155889171071	40.0	38.0	41.0	33.0	41.0
48-49	38.22789881174847	40.0	38.0	41.0	33.0	41.0
50-51	38.13762221649674	40.0	37.5	41.0	33.0	41.0
52-53	38.058830752436826	40.0	37.0	41.0	33.0	41.0
54-55	37.974779869583884	40.0	37.0	41.0	33.0	41.0
56-57	38.01457483421679	40.0	37.0	41.0	33.0	41.0
58-59	37.815291561338576	40.0	36.5	41.0	32.5	41.0
60-61	37.72284912683551	39.5	36.0	41.0	32.0	41.0
62-63	37.49787824123595	39.0	35.5	41.0	32.0	41.0
64-65	37.27849314108717	39.0	35.0	41.0	32.0	41.0
66-67	36.99708587848605	38.5	35.0	41.0	31.5	41.0
68-69	36.75612831088769	38.0	35.0	40.0	31.0	41.0
70-71	36.46183826371899	37.0	35.0	40.0	31.0	41.0
72-73	36.13602528901486	37.0	35.0	39.0	31.0	41.0
74-75	35.80365824054006	36.0	35.0	39.0	31.0	41.0
76-77	35.480872933648754	36.0	34.5	39.0	31.0	40.5
78-79	35.14398028077308	35.0	34.0	37.5	30.5	39.5
80-81	34.83769560559844	35.0	34.0	37.0	30.0	39.0
82-83	34.53784509686621	35.0	34.0	36.5	30.0	39.0
84-85	34.32323967751469	35.0	34.0	36.0	30.0	37.5
86-87	34.11091197026937	35.0	34.0	36.0	30.0	37.0
88-89	33.88630891052031	35.0	34.0	35.5	30.0	37.0
90-91	33.72042328319205	35.0	34.0	35.0	30.0	36.0
92-93	33.62814589515327	35.0	34.0	35.0	30.0	36.0
94-95	33.601370233923845	35.0	34.0	35.0	30.0	36.0
96-97	33.55514761299544	35.0	34.0	35.0	30.0	36.0
98-99	33.58906137010824	35.0	34.0	35.0	30.0	36.0
100	33.008112999859634	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	6.0
15	14.0
16	55.0
17	405.0
18	2189.0
19	6034.0
20	11852.0
21	18868.0
22	26361.0
23	35024.0
24	43810.0
25	54228.0
26	61688.0
27	65811.0
28	75390.0
29	89771.0
30	104611.0
31	126038.0
32	162547.0
33	219793.0
34	312634.0
35	470095.0
36	775128.0
37	1418721.0
38	2118814.0
39	1480482.0
40	98760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.946339757787825	20.725968070979658	14.594884003738207	23.73280816749431
2	14.31771169241815	21.28539864654276	41.64557997154839	22.7513096894907
3	17.87725463751915	26.475266885956593	30.489956782699196	25.157521693825064
4	12.329286627487305	18.68441672221326	39.36709666264074	29.619199987658696
5	13.389047698327348	38.38040513655505	35.36451426488728	12.866032900230318
6	29.288036561803075	38.19921112219738	19.419453200539802	13.09329911545974
7	26.182950660098314	33.153111159304046	23.378267180379993	17.28567100021764
8	24.135579701844375	36.759684259825995	21.52480561286153	17.5799304254681
9	25.039807248840233	17.981512158662323	21.66929734324377	35.30938324925367
10-11	23.780730686454103	27.38882182277659	29.119478902199482	19.71096858856983
12-13	24.5396072823072	24.915250739983158	28.983163796244888	21.561978181464756
14-15	21.91568360835569	26.436200594418015	26.51389351512547	25.134222282100826
16-17	21.698451510466505	29.35603432311906	27.18576815806113	21.759746008353304
18-19	21.73854494305358	28.99788222191926	28.266311284645457	20.997261550381708
20-21	22.786296046407074	28.039900615077666	27.903873452095272	21.269929886419987
22-23	22.532617789573894	27.968124217339142	27.582050153641653	21.91720783944531
24-25	22.241872357316005	28.140433242306656	27.677125069107678	21.94056933126966
26-27	22.224342010565408	28.20496674366079	27.626901107658586	21.943790138115215
28-29	22.085902532782512	28.357610465695913	27.639127355554027	21.91735964596754
30-31	22.42039944675252	28.113435159188153	27.55938474528859	21.906780648770734
32-33	22.18052752498397	28.15520827413764	27.59946419438378	22.064800006494615
34-35	22.36564489420807	28.16910672516392	27.604120831232642	21.86112754939536
36-37	22.315979007604707	28.00686426406506	27.665753890218024	22.011402838112204
38-39	22.125239023195775	28.095302173514735	27.79282570761184	21.98663309567765
40-41	22.36037651092977	27.979801540288886	27.72066599501325	21.9391559537681
42-43	22.254539456355328	28.008622325398548	27.614693376038602	22.12214484220753
44-45	22.310219596386684	28.05843733970467	27.542710336937198	22.088632726971444
46-47	22.273553414101034	28.027725366270932	27.470547828397063	22.228173391230968
48-49	22.161657352008017	28.027867150482024	27.66066749605267	22.149808001457284
50-51	22.09528713031172	28.104321105257934	27.657295437332486	22.14309632709786
52-53	22.183142728427853	28.010450654497475	27.536453963759854	22.26995265331482
54-55	22.22520249814184	27.94757847071186	27.58724411892609	22.239974912220205
56-57	22.236141757647367	27.994816222000374	27.555523355235472	22.213518665116787
58-59	22.237938836120446	28.10894433094841	27.585548801915706	22.067568031015437
60-61	22.251084102118487	27.982144157770332	27.62921623955441	22.137555500556772
62-63	22.252905660614918	28.057162989521927	27.507620777882625	22.18231057198053
64-65	22.15835784931672	28.067916236296863	27.546223010545507	22.227502903840907
66-67	22.23525050053624	28.031105550054036	27.456401539210123	22.2772424101996
68-69	22.181115087065027	27.950642017398042	27.589783519661776	22.27845937587515
70-71	22.15198739074366	27.808874241772934	27.61988823613698	22.41925013134642
72-73	22.267786165726537	27.819689187397245	27.63245174868795	22.280072898188273
74-75	22.139278274098455	27.98750376697326	27.601285970639132	22.271931988289158
76-77	22.212343928804202	27.883366090309348	27.552993030691862	22.35129695019459
78-79	22.224855309119246	27.92356385200167	27.505892443111723	22.345688395767365
80-81	22.18569319440475	27.930326368108904	27.582867181135367	22.30111325635098
82-83	22.191603122644917	27.920390523431525	27.66181931111949	22.226187042804064
84-85	22.37831322838596	27.910579672731235	27.556159402377762	22.154947696505044
86-87	22.1094582473462	27.98271658641201	27.644449952116968	22.26337521412482
88-89	22.202156020402747	27.92957319631052	27.6162807620724	22.251990021214336
90-91	22.2039789488573	28.034564062126137	27.61629710281798	22.14515988619858
92-93	22.209995775846117	28.012114241522596	27.641938232757674	22.135951749873612
94-95	22.06885289699698	28.111018783286045	27.677615653468536	22.142512666248436
96-97	22.06791488832574	28.30547922452042	27.731146305854836	21.895459581299008
98-99	23.19831048511559	30.131174361870023	27.9240667737753	18.746448379239087
100	22.180127297921672	31.11388319872174	21.623064331780895	25.08292517157569
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3365.0
1	2599.5
2	1948.5
3	2051.0
4	2035.0
5	2196.0
6	2608.5
7	3090.5
8	3465.0
9	3764.5
10	4083.0
11	4352.0
12	4656.5
13	5197.5
14	6087.0
15	7350.0
16	8902.5
17	10609.0
18	12632.0
19	15098.5
20	18105.0
21	22074.0
22	27551.0
23	34944.5
24	44851.0
25	57740.0
26	74109.0
27	92203.0
28	110996.0
29	131518.0
30	150859.0
31	169140.5
32	187498.0
33	204895.0
34	222780.5
35	239049.5
36	254901.5
37	269679.0
38	280177.0
39	288094.5
40	298306.5
41	306545.5
42	310523.0
43	316139.5
44	322035.0
45	328648.5
46	333785.5
47	336231.0
48	334913.0
49	328029.0
50	317791.0
51	307985.5
52	297331.0
53	283866.5
54	268822.5
55	250111.0
56	229141.0
57	206986.5
58	184284.0
59	161757.5
60	137272.0
61	112771.0
62	90812.5
63	71088.0
64	54177.0
65	41389.0
66	31896.5
67	25023.5
68	19785.5
69	15144.5
70	11684.5
71	9187.0
72	7271.0
73	5724.5
74	4494.5
75	3532.0
76	2709.5
77	2085.0
78	1584.5
79	1160.5
80	866.0
81	645.0
82	498.0
83	363.0
84	262.5
85	198.5
86	165.0
87	136.0
88	86.5
89	56.5
90	45.0
91	40.5
92	35.5
93	25.5
94	17.5
95	14.0
96	13.0
97	11.0
98	7.0
99	8.5
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02203331220843462
3	0.012790633399878909
4	0.004820590477341296
5	0.009834004573776246
6	0.009499776967347249
7	0.0027380953911298567
8	0.009114129729159945
9	0.005090543544072409
10-11	0.0041328529025739386
12-13	0.009557624053075345
14-15	0.005514755506078443
16-17	0.0037472056643866347
18-19	0.005893975290295959
20-21	0.004701460173197677
22-23	0.010846313022791484
24-25	0.010096111502305901
26-27	0.024278835067436014
28-29	0.020297269074151893
30-31	0.0165869023551977
32-33	0.01301351177862432
34-35	0.009255863752642647
36-37	0.011220381925294396
38-39	0.005458756752327174
40-41	0.006378906516029774
42-43	0.004357490382276884
44-45	0.007826598675391955
46-47	0.01045554021004426
48-49	0.007442126671985801
50-51	0.01113534405897599
52-53	9.76009545931079E-4
54-55	0.003017702093715477
56-57	5.753406442792388E-4
58-59	0.0032500406972535436
60-61	0.0011233153892758892
62-63	0.007546458295607142
64-65	0.003948032652448031
66-67	0.004237124745436235
68-69	0.005576469414803184
70-71	9.628793945414367E-4
72-73	4.9455785445966E-4
74-75	0.0
76-77	4.74242168918105E-5
78-79	4.320306223305108E-4
80-81	2.1873621506144665E-4
82-83	0.0
84-85	0.0010136636037685353
86-87	0.0035742393108327106
88-89	0.022262391340314932
90-91	0.002893084372852721
92-93	0.0013942072453773868
94-95	0.001445344478106308
96-97	6.254006472896699E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3382.0
20-21	6341.0
22-23	7091.0
24-25	8314.0
26-27	10737.0
28-29	14745.0
30-31	22614.0
32-33	27034.0
34-35	34480.0
36-37	47126.0
38-39	51959.0
40-41	50946.0
42-43	55420.0
44-45	63168.0
46-47	63589.0
48-49	62958.0
50-51	61885.0
52-53	62568.0
54-55	67838.0
56-57	70334.0
58-59	69926.0
60-61	68822.0
62-63	67695.0
64-65	70822.0
66-67	73558.0
68-69	74463.0
70-71	73331.0
72-73	70945.0
74-75	72438.0
76-77	75809.0
78-79	77204.0
80-81	78633.0
82-83	75850.0
84-85	78887.0
86-87	95575.0
88-89	85457.0
90-91	88246.0
92-93	93595.0
94-95	114382.0
96-97	267842.0
98-99	475416.0
100-101	4737705.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.4922090902735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.89815382940792	76.85591764013316
2	6.969217407331409	11.916275835663013
3	1.7699273446294084	4.539449958649498
4	0.6614250637397797	2.2618675938715493
5	0.2989039484254773	1.2776979428349615
6	0.13331935142636053	0.6838659520753242
7	0.08369582790129983	0.5008738853245032
8	0.05325183784493305	0.36420938043842854
9	0.03424502183016893	0.26349143099452044
>10	0.09637117634972166	1.2041434108161377
>50	0.0012409792220043287	0.0703446109904269
>100	2.0159290584674227E-4	0.028104678339021617
>500	2.330952946955545E-5	0.01245489479504454
>1k	2.330945617687025E-5	0.021302785074400546
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5709815879153582E-5	0.0	1.2854907939576791E-5	0.0
3	0.0	2.5709815879153582E-5	0.0	2.5709815879153582E-5	0.0
4	0.0	6.427453969788396E-5	0.0	3.8564723818730375E-5	1.2854907939576791E-5
5	0.0	6.427453969788396E-5	0.0	3.8564723818730375E-5	1.2854907939576791E-5
6	0.0	6.427453969788396E-5	0.0	3.8564723818730375E-5	1.2854907939576791E-5
7	0.0	7.712944763746075E-5	0.0	3.8564723818730375E-5	1.2854907939576791E-5
8	0.0	7.712944763746075E-5	0.0	3.8564723818730375E-5	3.8564723818730375E-5
9	0.0	1.2854907939576792E-4	0.0	3.8564723818730375E-5	3.8564723818730375E-5
10-11	0.0	1.2854907939576792E-4	0.0	8.355690160724914E-5	3.8564723818730375E-5
12-13	0.0	1.2854907939576792E-4	0.0	8.998435557703754E-5	3.8564723818730375E-5
14-15	1.2854907939576791E-5	1.478314413051331E-4	0.0	1.478314413051331E-4	5.7847085728095565E-5
16-17	2.5709815879153582E-5	1.542588952749215E-4	0.0	2.0567852703322866E-4	7.712944763746075E-5
18-19	4.499217778851877E-5	1.671138032144983E-4	0.0	2.4424325085195905E-4	7.712944763746075E-5
20-21	6.427453969788396E-5	1.8639616512386347E-4	0.0	2.956628826102662E-4	7.712944763746075E-5
22-23	8.355690160724914E-5	2.1853343497280544E-4	0.0	3.5993742230815015E-4	9.641180954682593E-5
24-25	1.7354125718428668E-4	2.2496088894259384E-4	0.0	4.949139556737064E-4	1.2212162542597953E-4
26-27	2.5709815879153584E-4	2.3138834291238223E-4	0.0	8.61278831951645E-4	1.2854907939576792E-4
28-29	2.5709815879153584E-4	2.3138834291238223E-4	0.0	0.0033551309722295425	1.2854907939576792E-4
30-31	2.5709815879153584E-4	3.08517790549843E-4	0.0	0.01145372297416292	1.2854907939576792E-4
32-33	2.76380520700901E-4	3.342276064289966E-4	0.0	0.03182875205839213	1.414039873353447E-4
34-35	3.213726984894198E-4	4.049296000966689E-4	0.0	0.05305220506663341	1.414039873353447E-4
36-37	3.342276064289966E-4	4.6277668582476447E-4	0.0	0.079642582139648	1.414039873353447E-4
38-39	3.342276064289966E-4	4.6277668582476447E-4	0.0	0.10690784187949037	1.414039873353447E-4
40-41	3.5993742230815015E-4	5.013414096434949E-4	0.0	0.12918539733877696	1.414039873353447E-4
42-43	3.5993742230815015E-4	5.270512255226485E-4	0.0	0.15127655663293965	1.478314413051331E-4
44-45	3.5993742230815015E-4	5.656159493413788E-4	0.0	0.1722429114823894	1.606863492447099E-4
46-47	3.8564723818730373E-4	5.784708572809556E-4	0.0	0.19370418028751288	2.3781579688217066E-4
48-49	3.8564723818730373E-4	5.784708572809556E-4	0.0	0.21063409404393552	2.956628826102662E-4
50-51	3.8564723818730373E-4	6.556003049184164E-4	0.0	0.22439527299325246	3.020903365800546E-4
52-53	3.8564723818730373E-4	6.556003049184164E-4	0.0	0.23640818446278697	3.4065506039878497E-4
54-55	3.8564723818730373E-4	6.620277588882048E-4	0.0	0.2474055582050949	3.7279233024772694E-4
56-57	3.8564723818730373E-4	6.684552128579931E-4	0.0	0.2584864888490101	3.7279233024772694E-4
58-59	3.985021461268805E-4	6.684552128579931E-4	0.0	0.2691367800769495	3.7279233024772694E-4
60-61	4.177845080362457E-4	6.748826668277815E-4	0.0	0.2789257924729372	3.7279233024772694E-4
62-63	4.370668699456109E-4	6.941650287371467E-4	0.0	0.28739717680511834	3.985021461268805E-4
64-65	4.499217778851877E-4	8.227141081329146E-4	0.0	0.29494300776564986	4.049296000966689E-4
66-67	4.6277668582476447E-4	8.29141562102703E-4	0.0	0.30221245820548054	4.306394159758225E-4
68-69	4.884865017039181E-4	8.61278831951645E-4	0.0	0.30973900680410277	4.5634923185497607E-4
70-71	4.884865017039181E-4	8.869886478307986E-4	0.0	0.3162050254977099	4.884865017039181E-4
72-73	4.884865017039181E-4	8.869886478307986E-4	0.0	0.32217613023564334	5.656159493413788E-4
74-75	4.884865017039181E-4	9.062710097401637E-4	0.0	0.3280508231640299	5.656159493413788E-4
76-77	5.077688636132833E-4	9.448357335588942E-4	0.0	0.33208726425705704	5.720434033111672E-4
78-79	5.141963175830717E-4	9.898279113474129E-4	0.0	0.3344782771338183	5.784708572809556E-4
80-81	5.141963175830717E-4	0.0010026828192869898	0.0	0.33551952467692403	6.041806731601092E-4
82-83	5.141963175830717E-4	0.0010026828192869898	0.0	0.3364193682326944	6.491728509486279E-4
84-85	5.141963175830717E-4	0.0010026828192869898	0.0	0.3366700389375161	6.556003049184164E-4
86-87	5.141963175830717E-4	0.0010155377272265665	0.0	0.33673431347721405	6.556003049184164E-4
88	5.141963175830717E-4	0.001105522082803604	0.0	0.33673431347721405	6.556003049184164E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8715	0.0	30.351646	1
GTATCAA	13265	0.0	26.29277	1
TCAACGC	14050	0.0	23.686008	4
ATCAACG	14145	0.0	23.439543	3
CAACGCA	14215	0.0	23.410772	5
AACGCAG	14780	0.0	22.60106	6
TATCAAC	15625	0.0	21.571001	2
GTACATG	24880	0.0	19.207674	1
ACGCAGA	17470	0.0	19.12185	7
TACATGG	24595	0.0	18.970667	2
CGCAGAG	17870	0.0	18.693708	8
ACATGGG	25620	0.0	17.783861	3
CATGGGG	18650	0.0	15.821278	4
GCAGAGT	21700	0.0	15.317147	9
AGAGTAC	17345	0.0	15.090867	10-11
GTACTAG	1940	0.0	14.045329	1
GGACCGA	1375	0.0	14.021679	6
CAGAGTA	21040	0.0	13.874951	10-11
GTATTAG	2640	0.0	13.496939	1
TATACTG	4565	0.0	13.496423	5
>>END_MODULE