FastQCFastQC Report
Fri 27 May 2016
SRR2140333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12059366
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173300.021.298571
GTATCAA239850.020.9913141
GTACATG358200.019.3544861
TCAACGC245350.019.2504984
ATCAACG247000.019.1571663
CAACGCA247450.019.120665
TACATGG351400.019.0125082
AACGCAG253750.018.877656
ACATGGG363600.017.8290313
TATCAAC277350.017.5630842
CATGGGG257800.016.6434564
CGCAGAG288650.016.4509818
ACGCAGA290150.016.3806087
AGAGTAC253300.014.19690910-11
GCAGAGT351250.013.3637389
ATGGGGG165350.013.1520075
GTACTAG28400.013.0114141
GTATAAT75650.012.9887421
GTGTAGC67750.012.5819191
TATACCG10350.012.5744165
Produced by FastQC (version 0.11.5)