##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140333_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12059366
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74994589267794	33.0	31.0	34.0	30.0	34.0
2	31.914099049651533	34.0	31.0	34.0	30.0	34.0
3	32.06360906535219	34.0	31.0	34.0	30.0	34.0
4	35.50969147134269	37.0	35.0	37.0	33.0	37.0
5	35.63252802842206	37.0	35.0	37.0	35.0	37.0
6	35.750954735099675	37.0	36.0	37.0	35.0	37.0
7	35.70089007995943	37.0	36.0	37.0	35.0	37.0
8	35.642034415407906	37.0	35.0	37.0	35.0	37.0
9	37.38269947192912	39.0	38.0	39.0	35.0	39.0
10-11	37.37109794992539	39.0	38.0	39.0	35.0	39.0
12-13	37.29885489004978	39.0	38.0	39.0	34.5	39.0
14-15	38.703127884169035	41.0	39.0	41.0	34.0	41.0
16-17	38.719419826879786	41.0	39.0	41.0	34.0	41.0
18-19	38.74562526753064	41.0	38.5	41.0	34.0	41.0
20-21	38.783466076962654	40.5	39.0	41.0	34.0	41.0
22-23	38.760795908747355	40.0	39.0	41.0	34.0	41.0
24-25	38.73064559809821	40.0	38.0	41.0	34.0	41.0
26-27	38.6797748474426	40.0	38.0	41.0	34.0	41.0
28-29	38.61441547197312	40.0	38.0	41.0	34.0	41.0
30-31	38.497374927115345	40.0	38.0	41.0	34.0	41.0
32-33	38.43707406661395	40.0	38.0	41.0	33.5	41.0
34-35	38.28946423667166	40.0	38.0	41.0	33.0	41.0
36-37	38.185260465745586	40.0	38.0	41.0	33.0	41.0
38-39	38.17789595417939	40.0	38.0	41.0	33.0	41.0
40-41	38.097288994019806	40.0	38.0	41.0	33.0	41.0
42-43	37.932535281980435	40.0	38.0	41.0	33.0	41.0
44-45	37.997530652219105	40.0	37.5	41.0	32.5	41.0
46-47	38.20517663113344	40.0	38.0	41.0	33.0	41.0
48-49	38.20034625854076	40.0	38.0	41.0	33.0	41.0
50-51	38.108400424550865	40.0	37.5	41.0	33.0	41.0
52-53	38.036678842731455	40.0	37.0	41.0	33.0	41.0
54-55	37.95834891856586	40.0	37.0	41.0	33.0	41.0
56-57	38.00976896537449	40.0	37.0	41.0	33.0	41.0
58-59	37.81659748120528	40.0	36.5	41.0	32.5	41.0
60-61	37.73276274938759	40.0	36.0	41.0	32.0	41.0
62-63	37.511519310541516	39.0	36.0	41.0	32.0	41.0
64-65	37.31386789788634	39.0	35.0	41.0	32.0	41.0
66-67	37.04692569934855	39.0	35.0	41.0	31.5	41.0
68-69	36.82056189560908	38.0	35.0	40.5	31.0	41.0
70-71	36.537755592211624	37.0	35.0	40.0	31.0	41.0
72-73	36.21932217448607	37.0	35.0	39.5	31.0	41.0
74-75	35.88695405040812	36.0	35.0	39.0	31.0	41.0
76-77	35.55968865435099	36.0	34.5	39.0	31.0	40.5
78-79	35.207388637513205	35.5	34.0	37.5	30.5	39.5
80-81	34.89225163659975	35.0	34.0	37.0	30.0	39.0
82-83	34.581614322672664	35.0	34.0	37.0	30.0	39.0
84-85	34.35428140899977	35.0	34.0	36.0	30.0	38.0
86-87	34.12867080952715	35.0	34.0	36.0	30.0	37.0
88-89	33.892425091810814	35.0	34.0	36.0	30.0	37.0
90-91	33.71709475611111	35.0	34.0	35.0	30.0	36.5
92-93	33.61913236618928	35.0	34.0	35.0	30.0	36.0
94-95	33.590420496855145	35.0	34.0	35.0	30.0	36.0
96-97	33.54226987960468	35.0	34.0	35.0	30.0	36.0
98-99	33.57229174754747	35.0	34.0	35.0	30.0	36.0
100	32.97957339174121	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	4.0
14	7.0
15	14.0
16	75.0
17	517.0
18	2885.0
19	8609.0
20	17350.0
21	28453.0
22	40922.0
23	53177.0
24	68199.0
25	84407.0
26	96721.0
27	103987.0
28	116409.0
29	139398.0
30	162782.0
31	197190.0
32	255609.0
33	343143.0
34	490135.0
35	737504.0
36	1211410.0
37	2176988.0
38	3281825.0
39	2295185.0
40	146460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.57004323444532	21.15882377232767	14.660405862132386	23.61072713109462
2	14.10936961912263	21.61618912952458	41.80719845687634	22.467242794476444
3	17.649320477595968	26.820009641885957	30.758968027934102	24.771701852583973
4	12.031510391788471	18.936279641541677	40.02373545786496	29.008474508804895
5	13.08054021719849	38.59486504894973	35.7705930418893	12.554001691962483
6	28.704536546991005	38.77957924406972	19.66206072845686	12.853823480482413
7	25.722621173381064	33.37716511867794	23.878236477858287	17.021977230082708
8	23.76646489575999	36.883821257348714	21.92184093353668	17.427872913354612
9	24.71473269484496	18.05923363151387	22.094373435257065	35.13166023838411
10-11	23.416338692048832	27.56881247481105	29.54924429009044	19.465604543049675
12-13	24.196514838055272	25.148909651740475	29.318802847962527	21.335772662241723
14-15	21.60570025560262	26.719937771366574	26.863294166086348	24.811067806944457
16-17	21.31844971731454	29.64414035362686	27.562934339561306	21.4744755894973
18-19	21.363008881708616	29.415243045879187	28.50995585371658	20.711792218695617
20-21	22.390093499913124	28.392522894542633	28.165361469326548	21.052022136217698
22-23	22.070640160466997	28.273484044655962	27.981905937584507	21.673969857292537
24-25	21.898846770238045	28.428815419854857	28.045415474559782	21.626922335347313
26-27	21.83559476300089	28.540897726687746	28.045187505074477	21.578320005236883
28-29	21.646868759343597	28.77972504467819	27.986093071579166	21.587313124399053
30-31	22.01860182740949	28.462947412000915	27.931300781993684	21.58714997859591
32-33	21.826286126974566	28.5027416292284	27.944527549077748	21.726444694719294
34-35	22.032683603735126	28.52649639702232	27.94549462492551	21.49532537431704
36-37	21.956041445346163	28.347087142368764	28.031219362221083	21.66565205006399
38-39	21.725172583414736	28.475992559381737	28.175607700841905	21.62322715636163
40-41	21.98105370255968	28.371782115654913	28.05441099835539	21.59275318343002
42-43	21.811536851429665	28.419169352620887	27.90211347874596	21.867180317203488
44-45	21.91208882446156	28.436505431240917	27.865022798098593	21.786382946198934
46-47	21.925876142190067	28.35301055420871	27.868407490888487	21.85270581271274
48-49	21.84283240608022	28.326301808492072	28.065864233486735	21.76500155194097
50-51	21.71149896965189	28.445807070430256	28.0579984553676	21.784695504550257
52-53	21.821995094856327	28.31228741854198	27.95806741376468	21.907650072837008
54-55	21.86863070127406	28.24220287520568	27.98891876945878	21.900247654061477
56-57	21.894236254820516	28.289682334401522	27.993820452390555	21.822260958387403
58-59	21.91434841603311	28.395523028767656	27.987407768332922	21.702720786866312
60-61	21.941344712358184	28.295350516869277	28.025689521128765	21.737615249643767
62-63	21.90725723950507	28.332950899767162	27.95790550441638	21.801886356311392
64-65	21.815505982994164	28.38872429589844	27.94527646907663	21.850493252030763
66-67	21.898662396370145	28.322365617168394	27.848309273355447	21.930662713106013
68-69	21.821605146886693	28.300293803990996	27.97308764963754	21.905013399484773
70-71	21.83602804994421	28.08951613271588	28.010612956122614	22.063842861217296
72-73	21.901739433852637	28.171872643682338	28.05326009714937	21.873127825315652
74-75	21.731640766149166	28.322846655007478	28.01459162496108	21.93092095388228
76-77	21.816280113798882	28.27564690781995	27.905450670401805	22.002622307979365
78-79	21.809915940463384	28.239114377640306	27.942429427462912	22.008540254433402
80-81	21.79142042724741	28.264395662366802	27.98048369989619	21.963700210489595
82-83	21.824240218209685	28.256785505976	28.11999088141205	21.798983394402267
84-85	21.998760048996306	28.24430107444283	27.96809670721633	21.788842169344534
86-87	21.74997637751555	28.33488056547628	28.03503466092237	21.880108396085802
88-89	21.837344481703123	28.272070456384096	28.008778912585537	21.88180614932724
90-91	21.856487080369174	28.34277570642165	28.0414728429321	21.75926437027707
92-93	21.815813993100814	28.320390516727734	28.062330735332207	21.801464754839245
94-95	21.658875122069645	28.419259244152485	28.11743734948873	21.80442828428915
96-97	21.71317792678439	28.561671530350164	28.19410404954466	21.531046493320787
98-99	22.76565579340754	30.47964889571636	28.36986131863682	18.38483399223928
100	21.755129419715175	31.43103580563179	22.157883405542535	24.6559513691105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4035.0
1	3148.5
2	2546.5
3	2781.0
4	2746.5
5	3017.0
6	3673.0
7	4443.5
8	5029.0
9	5575.5
10	6161.5
11	6517.5
12	6934.0
13	7691.5
14	8843.0
15	10604.5
16	12857.5
17	15556.5
18	18876.5
19	22949.5
20	28149.0
21	35153.0
22	44940.0
23	57859.0
24	74827.5
25	96982.0
26	124725.5
27	154670.0
28	185378.0
29	219464.5
30	252684.5
31	282162.0
32	311909.5
33	341111.5
34	370836.5
35	397366.5
36	423186.0
37	446904.5
38	460278.0
39	470282.0
40	483216.0
41	493462.0
42	498766.0
43	504217.0
44	508086.5
45	515148.5
46	522191.5
47	528284.0
48	527539.5
49	506592.0
50	478850.0
51	456174.5
52	434996.5
53	410111.5
54	381846.5
55	350476.0
56	317028.5
57	282125.5
58	247324.0
59	215034.5
60	181004.5
61	147961.5
62	118963.0
63	93502.0
64	71933.5
65	55069.0
66	42418.5
67	33340.0
68	26571.5
69	20707.5
70	16298.0
71	12977.5
72	10401.0
73	8202.5
74	6494.0
75	5233.0
76	4104.5
77	3222.5
78	2482.5
79	1825.5
80	1341.5
81	1005.5
82	780.0
83	526.0
84	360.0
85	288.5
86	244.5
87	203.0
88	144.0
89	89.0
90	61.5
91	53.5
92	46.5
93	32.0
94	20.5
95	17.5
96	16.5
97	13.0
98	8.5
99	16.5
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.023069206125761504
3	0.012927711125112215
4	0.0050914782750602315
5	0.009776633365302953
6	0.009652248716889428
7	0.0031925393092804384
8	0.00942006403985085
9	0.005605601488502795
10-11	0.004195908806482862
12-13	0.009382748645326795
14-15	0.005045870570641939
16-17	0.0038849471854490525
18-19	0.00585851694027696
20-21	0.004609148558334252
22-23	0.010562917118967678
24-25	0.009849406727011808
26-27	0.02352381507518738
28-29	0.020121504050135006
30-31	0.016066668322880746
32-33	0.013031840530137952
34-35	0.009271764279305048
36-37	0.010696145519602955
38-39	0.0055658348830926125
40-41	0.006688342770211507
42-43	0.004106900289885169
44-45	0.007291354908109843
46-47	0.010248132526068003
48-49	0.007538565009227794
50-51	0.010604876535038084
52-53	0.0010765631528766777
54-55	0.002860197915739384
56-57	5.986480881030847E-4
58-59	0.0030740636083764817
60-61	0.001165513445502969
62-63	0.00754139150630304
64-65	0.003827106085003592
66-67	0.0042040690680020905
68-69	0.005673576691237108
70-71	8.742385713566537E-4
72-73	6.604163145273912E-4
74-75	0.0
76-77	2.0311432142323423E-5
78-79	4.4201359808577275E-4
80-81	2.1334589958027056E-4
82-83	0.0
84-85	0.0010082101916607574
86-87	0.003652828076318456
88-89	0.022289059489043778
90-91	0.003172447499757327
92-93	0.0013930005685254352
94-95	0.0013019824410960964
96-97	6.137102395429086E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4855.0
20-21	9692.0
22-23	10756.0
24-25	12838.0
26-27	16893.0
28-29	21148.0
30-31	33791.0
32-33	41026.0
34-35	51399.0
36-37	69412.0
38-39	76733.0
40-41	76632.0
42-43	83734.0
44-45	94788.0
46-47	96555.0
48-49	95307.0
50-51	93511.0
52-53	95773.0
54-55	105832.0
56-57	109230.0
58-59	107971.0
60-61	104372.0
62-63	103308.0
64-65	108665.0
66-67	113335.0
68-69	113209.0
70-71	112132.0
72-73	108979.0
74-75	111995.0
76-77	117406.0
78-79	119331.0
80-81	119026.0
82-83	116644.0
84-85	121998.0
86-87	147925.0
88-89	130973.0
90-91	137167.0
92-93	145795.0
94-95	177659.0
96-97	411353.0
98-99	740347.0
100-101	7389871.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.0929760943118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.76475597800561	73.7572774649784
2	7.2876803285427325	12.111100946451751
3	2.1234856319776223	5.293402225635934
4	0.8333549321189414	2.769837658105441
5	0.3822969775712848	1.5883096809129207
6	0.21428517881081646	1.06833559441755
7	0.1236591980783758	0.7192647552837773
8	0.07488677730637242	0.49780521571987635
9	0.052132997228139036	0.3898697303162322
>10	0.14186101841174645	1.6609996571370367
>50	0.0012612795632984547	0.06592683601772956
>100	3.061632601060642E-4	0.045153954115623604
>500	1.6771175359423352E-5	0.008445015251677297
>1k	1.6767949562514983E-5	0.024271265656030067
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.292309894234905E-6	0.0	0.0	0.0
3	0.0	2.487692968270471E-5	0.0	8.292309894234905E-6	0.0
4	0.0	7.463078904811414E-5	0.0	3.316923957693962E-5	0.0
5	0.0	8.292309894234904E-5	0.0	3.316923957693962E-5	0.0
6	0.0	8.292309894234904E-5	0.0	3.316923957693962E-5	0.0
7	0.0	8.292309894234904E-5	0.0	4.146154947117452E-5	0.0
8	0.0	9.121540883658394E-5	0.0	4.975385936540942E-5	0.0
9	0.0	1.3267695830775848E-4	0.0	7.463078904811414E-5	0.0
10-11	0.0	1.4096926820199337E-4	0.0	9.950771873081885E-5	0.0
12-13	0.0	1.4096926820199337E-4	0.0	1.8243081767316792E-4	0.0
14-15	0.0	2.6535391661551696E-4	0.0	2.860846913511042E-4	1.658461978846981E-5
16-17	0.0	2.8193853640398674E-4	0.0	4.2290780460598013E-4	3.316923957693962E-5
18-19	0.0	2.943770012453391E-4	0.0	5.224155233367991E-4	4.560770441829197E-5
20-21	0.0	3.1510777598092634E-4	0.0	7.255771157455541E-4	4.975385936540942E-5
22-23	0.0	3.897385650290405E-4	0.0	0.0010282464268851282	5.804616925964433E-5
24-25	0.0	4.519308892358023E-4	0.0	0.0013972542171785812	7.463078904811414E-5
26-27	0.0	4.8510012881274186E-4	0.0	0.0017538235426306822	7.463078904811414E-5
28-29	0.0	5.016847486012117E-4	0.0	0.0036652009732518276	7.463078904811414E-5
30-31	0.0	5.59730917860856E-4	0.0	0.009556887153105726	7.463078904811414E-5
32-33	0.0	5.721693827022084E-4	0.0	0.022973844561977805	9.121540883658394E-5
34-35	0.0	6.592386365916749E-4	0.0	0.03476136307663272	9.121540883658394E-5
36-37	0.0	7.504540454282588E-4	0.0	0.050243105649169284	9.121540883658394E-5
38-39	4.1461549471174525E-6	7.79477130058081E-4	0.0	0.06702259472015362	9.121540883658394E-5
40-41	8.292309894234905E-6	8.997156235244872E-4	0.0	0.08132682928770882	9.950771873081885E-5
42-43	8.292309894234905E-6	9.411771729956617E-4	0.0	0.09544034072769664	9.950771873081885E-5
44-45	8.292309894234905E-6	0.001082146441197655	0.0	0.10945849060390074	9.950771873081885E-5
46-47	8.292309894234905E-6	0.001181654159928474	0.0	0.12527192557220668	1.036538736779363E-4
48-49	8.292309894234905E-6	0.0011899464698227088	0.0	0.1372667518342175	1.2438464841352356E-4
50-51	8.292309894234905E-6	0.0013184772731833498	0.0	0.14771920845590059	1.2438464841352356E-4
52-53	8.292309894234905E-6	0.0013350618929718196	0.0	0.15843287283925206	1.4096926820199337E-4
54-55	8.292309894234905E-6	0.0013516465127602893	0.0	0.16886459868619957	1.6170004293758063E-4
56-57	8.292309894234905E-6	0.0013682311325487592	0.0	0.17920925527925763	1.6584619788469809E-4
58-59	8.292309894234905E-6	0.0013806695973901115	0.0	0.18850493467069496	1.6584619788469809E-4
60-61	8.292309894234905E-6	0.001422131146861286	0.0	0.19673090608577598	1.74138507778933E-4
62-63	8.292309894234905E-6	0.001438715766649756	0.0	0.20488639286675603	1.990154374616377E-4
64-65	8.292309894234905E-6	0.0015755388799046318	0.0	0.21215045633410579	1.990154374616377E-4
66-67	8.292309894234905E-6	0.0015879773447459841	0.0	0.2193730582519844	1.990154374616377E-4
68-69	8.292309894234905E-6	0.0016418773590585111	0.0	0.2264837139862908	1.990154374616377E-4
70-71	8.292309894234905E-6	0.0016750465986354506	0.0	0.23228003860236102	2.0316159240875515E-4
72-73	8.292309894234905E-6	0.0016916312184239205	0.0	0.23820904017673897	2.6535391661551696E-4
74-75	8.292309894234905E-6	0.0017372389228422125	0.0	0.24324661843748668	2.6535391661551696E-4
76-77	8.292309894234905E-6	0.0018201620217845615	0.0	0.24689938094589714	2.6535391661551696E-4
78-79	8.292309894234905E-6	0.001861623571255736	0.0	0.24901806612387417	2.6535391661551696E-4
80-81	8.292309894234905E-6	0.0018657697262028535	0.0	0.24996338945181695	2.777923814568693E-4
82-83	8.292309894234905E-6	0.0019030851207269103	0.0	0.25077603582145197	2.9852315619245657E-4
84-85	8.292309894234905E-6	0.0019362543603038502	0.0	0.2510704128226973	2.9852315619245657E-4
86-87	8.292309894234905E-6	0.0019652774449336725	0.0	0.251165774386481	2.9852315619245657E-4
88	8.292309894234905E-6	0.002089662093347196	0.0	0.25117406669637526	2.9852315619245657E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17330	0.0	21.29857	1
GTATCAA	23985	0.0	20.991314	1
GTACATG	35820	0.0	19.354486	1
TCAACGC	24535	0.0	19.250498	4
ATCAACG	24700	0.0	19.157166	3
CAACGCA	24745	0.0	19.12066	5
TACATGG	35140	0.0	19.012508	2
AACGCAG	25375	0.0	18.87765	6
ACATGGG	36360	0.0	17.829031	3
TATCAAC	27735	0.0	17.563084	2
CATGGGG	25780	0.0	16.643456	4
CGCAGAG	28865	0.0	16.450981	8
ACGCAGA	29015	0.0	16.380608	7
AGAGTAC	25330	0.0	14.196909	10-11
GCAGAGT	35125	0.0	13.363738	9
ATGGGGG	16535	0.0	13.152007	5
GTACTAG	2840	0.0	13.011414	1
GTATAAT	7565	0.0	12.988742	1
GTGTAGC	6775	0.0	12.581919	1
TATACCG	1035	0.0	12.574416	5
>>END_MODULE