FastQCFastQC Report
Fri 27 May 2016
SRR2140332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7032443
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.039.8919871
GTATCAA96150.028.9046121
TCAACGC103100.025.3980794
ATCAACG104450.025.0717743
CAACGCA106100.024.8017925
AACGCAG112250.023.6364026
TATCAAC116000.023.212232
GTACATG209550.020.2450941
GTGTAGA108900.020.18186894
TACATGG210450.019.8295362
ACGCAGA132400.019.7790557
TGTAGAT105950.019.5637694
CGCAGAG135500.019.358358
ACATGGG212150.018.668793
GGTGTAG119550.018.43902694
AGGTGTA144150.016.38787394
CATGGGG156200.016.2652684
GTGTAGC39400.015.4918861
TATACCG6150.015.482555
GCAGAGT174050.014.9715519
Produced by FastQC (version 0.11.5)