##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7032443
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03863095655379	33.0	31.0	34.0	30.0	34.0
2	32.07672511529777	34.0	31.0	34.0	30.0	34.0
3	32.27302489334076	34.0	31.0	34.0	30.0	34.0
4	35.686504817742566	37.0	35.0	37.0	35.0	37.0
5	35.762294838365555	37.0	35.0	37.0	35.0	37.0
6	35.816527058946654	37.0	36.0	37.0	35.0	37.0
7	35.72300621562094	37.0	36.0	37.0	35.0	37.0
8	35.68275818232725	37.0	36.0	37.0	35.0	37.0
9	37.38540390586884	39.0	38.0	39.0	35.0	39.0
10-11	37.38525786842496	39.0	38.0	39.0	35.0	39.0
12-13	37.32238583377071	39.0	38.0	39.0	34.5	39.0
14-15	38.72639756909512	41.0	39.0	41.0	34.0	41.0
16-17	38.74094784699997	41.0	38.5	41.0	34.0	41.0
18-19	38.76097004981057	41.0	38.5	41.0	34.0	41.0
20-21	38.80623658855664	40.5	39.0	41.0	34.0	41.0
22-23	38.77217474039669	40.0	39.0	41.0	34.0	41.0
24-25	38.739175560964426	40.0	38.0	41.0	34.0	41.0
26-27	38.6941487061744	40.0	38.0	41.0	34.0	41.0
28-29	38.620750086705144	40.0	38.0	41.0	34.0	41.0
30-31	38.49917755115057	40.0	38.0	41.0	34.0	41.0
32-33	38.44230934603865	40.0	38.0	41.0	34.0	41.0
34-35	38.29025356926901	40.0	38.0	41.0	33.0	41.0
36-37	38.174152621425975	40.0	38.0	41.0	33.0	41.0
38-39	38.15769549238027	40.0	38.0	41.0	33.0	41.0
40-41	38.070433199166686	40.0	38.0	41.0	33.0	41.0
42-43	37.89222210758902	40.0	37.5	41.0	32.5	41.0
44-45	37.95641313032391	40.0	37.5	41.0	32.5	41.0
46-47	38.157476186362274	40.0	38.0	41.0	33.0	41.0
48-49	38.147632802579565	40.0	38.0	41.0	33.0	41.0
50-51	38.06238946741535	40.0	37.5	41.0	33.0	41.0
52-53	37.98514737928504	40.0	37.0	41.0	33.0	41.0
54-55	37.9074850435575	40.0	37.0	41.0	33.0	41.0
56-57	37.9598023348247	40.0	37.0	41.0	33.0	41.0
58-59	37.77524540117469	40.0	36.5	41.0	32.0	41.0
60-61	37.69790111106582	39.5	36.0	41.0	32.0	41.0
62-63	37.47867284262777	39.0	36.0	41.0	32.0	41.0
64-65	37.274630683919526	39.0	35.0	41.0	32.0	41.0
66-67	37.001550808118914	39.0	35.0	41.0	31.0	41.0
68-69	36.77541488128783	38.0	35.0	40.0	31.0	41.0
70-71	36.48852537436768	37.0	35.0	40.0	31.0	41.0
72-73	36.164847879951694	37.0	35.0	39.5	31.0	41.0
74-75	35.81548937228703	36.0	35.0	39.0	31.0	41.0
76-77	35.4696522613587	36.0	34.0	39.0	30.5	40.5
78-79	35.10457752685576	35.0	34.0	37.5	30.0	39.5
80-81	34.77416683286472	35.0	34.0	37.0	30.0	39.0
82-83	34.445215591551396	35.0	34.0	37.0	30.0	39.0
84-85	34.21083370537832	35.0	34.0	36.0	30.0	37.5
86-87	33.99369893827489	35.0	34.0	36.0	30.0	37.0
88-89	33.784362606717025	35.0	34.0	35.5	29.5	37.0
90-91	33.6039990268939	35.0	34.0	35.0	29.0	36.0
92-93	33.506345354994025	35.0	33.0	35.0	29.0	36.0
94-95	33.47904386763673	35.0	33.0	35.0	29.0	36.0
96-97	33.43196185094338	35.0	33.0	35.0	29.0	36.0
98-99	33.46706526176305	35.0	33.0	35.0	29.0	35.5
100	32.84845566696905	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	33.0
17	208.0
18	1108.0
19	3411.0
20	7584.0
21	13343.0
22	20688.0
23	28405.0
24	37414.0
25	47539.0
26	56489.0
27	62286.0
28	71501.0
29	86797.0
30	103163.0
31	123850.0
32	160091.0
33	213934.0
34	303549.0
35	449395.0
36	727825.0
37	1303876.0
38	1931343.0
39	1206212.0
40	72392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.770611293969964	20.88110774591419	14.717104710269247	23.631176249846604
2	14.157535699062157	21.21752935281507	41.984335778198236	22.640599169924535
3	17.72191431011032	26.536338590941334	30.592164956194818	25.14958214275353
4	12.204192204320188	18.600176334238704	39.711736181226094	29.483895280215016
5	13.282612121098353	38.37086868004253	35.564549021214	12.781970177645121
6	29.156634284785444	38.40050922855884	19.559237425682397	12.883619060973322
7	26.112021755740226	33.09706027607914	23.532039988857065	17.25887797932357
8	23.91549228074452	37.109068747528205	21.600533346151238	17.37490562557604
9	24.82408391526449	18.127057189330102	21.811646283314985	35.237212612090424
10-11	23.59551768132825	27.48756286116194	29.297581797011564	19.61933766049825
12-13	24.267443916738525	25.008527333579405	29.22864197137589	21.49538677830618
14-15	21.717188451442972	26.57213041599913	26.65560498299081	25.055076149567086
16-17	21.526238180150393	29.5248681935367	27.322487440071086	21.626406186241816
18-19	21.561750819266027	29.150000430172877	28.41490963560944	20.87333911495165
20-21	22.513887332710862	28.21032412965186	28.138181494820152	21.137607042817127
22-23	22.309058998983076	28.10020962139716	27.882920711511005	21.707810668108756
24-25	21.964877262498888	28.305060884275417	27.940144236797764	21.789917616427925
26-27	21.977805189344863	28.401034317118544	27.857272511036246	21.76388798250034
28-29	21.89662015283341	28.475487656889037	27.90857844533914	21.71931374493841
30-31	22.156946849458375	28.305335656644754	27.829568539666	21.708148954230868
32-33	21.926854860246	28.328729032167388	27.841010626656338	21.903405480930278
34-35	22.12371863093147	28.365229277634256	27.836612838304596	21.67443925312968
36-37	22.114407254002014	28.200221722283814	27.88007496668749	21.805296057026684
38-39	21.931229105762583	28.265215390444236	28.03158766507183	21.771967838721356
40-41	22.157132500879793	28.09322940236872	27.980994301196834	21.76864379555466
42-43	21.956854412214533	28.197551570144668	27.920605177197466	21.924988840443334
44-45	22.04954204684814	28.283227126582407	27.85090227799838	21.816328548571082
46-47	21.974337236058698	28.161640549590356	27.82108839466674	22.0429338196842
48-49	21.856926815472416	28.117726091800193	28.009495652741535	22.015851439985852
50-51	21.769357376481242	28.1283794512523	28.02570277549557	22.076560396770883
52-53	21.863564789360648	27.942915971361625	28.01693523310827	22.176584006169453
54-55	21.899476428876824	27.750183396791805	28.150639062198724	22.199701112132647
56-57	21.78426187154462	27.675419956217162	28.23144294092131	22.30887523131691
58-59	21.630873751222886	27.686564115809002	28.37971721402821	22.302844918939897
60-61	21.567492689587812	27.530748073537826	28.66771913924832	22.234040097626046
62-63	21.46106796595191	27.70667046066004	28.655918153381936	22.176343420006116
64-65	21.270630169289287	27.976776120004192	28.39118407026571	22.361409640440815
66-67	21.19198334419886	28.147094892328074	28.147156973662224	22.51376478981084
68-69	21.2767376410313	28.320473644482004	28.1301174393556	22.272671275131096
70-71	21.27354400788862	28.38324431089892	27.81539088423569	22.527820796976766
72-73	21.616663803467446	28.292967553704173	27.465624222329645	22.62474442049874
74-75	21.887670858421256	28.094298049630833	27.465869891806182	22.55216120014173
76-77	22.066257271586004	27.679771991187184	27.539876623558296	22.714094113668516
78-79	22.36507323959457	27.461244528881895	27.4110108416369	22.762671389886634
80-81	22.67160848940626	27.45885122721542	27.21204366205312	22.6574966213252
82-83	22.424744878910662	27.74870514693704	27.08151632320201	22.74503365095029
84-85	22.82466857852811	27.744647155026215	26.992331895595772	22.438352370849902
86-87	22.666823478871848	27.97959848979115	27.052104489891715	22.301473541445286
88-89	22.685947310482796	28.185462323135503	26.938124923431715	22.19046544294999
90-91	22.765553557601255	28.315902674060965	27.294113392413276	21.6244303759245
92-93	22.895417807114075	28.049118644877158	27.582150198280488	21.473313349728283
94-95	22.541990276836543	28.116635272439755	27.99348109013808	21.34789336058562
96-97	22.800495125577307	27.858619587627892	28.252099240059543	21.08878604673525
98-99	23.587257705797136	29.88902478871656	28.677146109088568	17.846571396397735
100	22.792545548345995	31.023405201963893	22.601245957605933	23.58280329208418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2203.0
1	1738.5
2	1403.5
3	1511.0
4	1496.5
5	1658.0
6	2042.0
7	2411.5
8	2676.5
9	2961.0
10	3232.0
11	3463.0
12	3759.5
13	4268.5
14	5080.0
15	6107.0
16	7290.5
17	8641.5
18	10333.0
19	12425.5
20	14855.5
21	18312.0
22	23449.0
23	29868.0
24	38307.0
25	49712.5
26	63729.0
27	79623.5
28	95791.0
29	113411.5
30	130847.5
31	146943.5
32	163241.5
33	178455.0
34	193946.0
35	209559.5
36	225184.5
37	240307.0
38	251467.5
39	258929.5
40	266854.5
41	273926.5
42	278718.0
43	283303.5
44	288031.5
45	292403.0
46	293097.5
47	291420.5
48	288322.0
49	282116.5
50	274843.0
51	267091.0
52	256908.5
53	242735.0
54	226941.0
55	209827.0
56	189918.5
57	168367.0
58	147684.5
59	128434.0
60	108043.5
61	87713.5
62	70155.5
63	55063.0
64	42107.5
65	32120.5
66	24813.5
67	19473.0
68	15366.0
69	11847.5
70	9145.0
71	7295.0
72	5927.0
73	4750.5
74	3744.5
75	2946.0
76	2229.5
77	1673.0
78	1253.0
79	940.5
80	747.5
81	621.5
82	512.5
83	369.0
84	259.5
85	192.0
86	150.5
87	121.5
88	87.0
89	65.0
90	51.0
91	43.5
92	34.0
93	16.0
94	7.5
95	8.5
96	5.5
97	6.0
98	7.5
99	11.5
100	29.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.021557231249510308
3	0.01215793714929506
4	0.004877394669249364
5	0.009541492195528637
6	0.00895848000474373
7	0.0026164449537664225
8	0.008702523433179623
9	0.005645264383941683
10-11	0.003967326859243651
12-13	0.009150447433416808
14-15	0.00513335124081347
16-17	0.003661600954319857
18-19	0.00549595638386262
20-21	0.004410458620116884
22-23	0.010393133811740198
24-25	0.009090207054399618
26-27	0.022718147157546928
28-29	0.019469208130118563
30-31	0.015478080277449595
32-33	0.012813049434781041
34-35	0.008760115668049441
36-37	0.010564997736432987
38-39	0.005256637921182668
40-41	0.006265318722532182
42-43	0.004110570530306906
44-45	0.0074726710316812455
46-47	0.009974797655431987
48-49	0.007455352096967483
50-51	0.01032900201844035
52-53	9.424794251086622E-4
54-55	0.003481642229465448
56-57	6.808398356059263E-4
58-59	0.0029252186051481575
60-61	7.896519153614631E-4
62-63	0.007056085900759276
64-65	0.0034514227241057203
66-67	0.0038333754306242467
68-69	0.005273897475276353
70-71	8.944114088269857E-4
72-73	5.595797779699357E-4
74-75	0.0
76-77	1.6313374707858045E-5
78-79	3.628158600665404E-4
80-81	1.1676472957872454E-4
82-83	0.0
84-85	0.0010756025679243021
86-87	0.003599974004033875
88-89	0.022143363439937266
90-91	0.0030106474486410713
92-93	0.0015103238776507427
94-95	0.001306414569135275
96-97	5.373573163131217E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2511.0
20-21	4765.0
22-23	5147.0
24-25	6201.0
26-27	7971.0
28-29	11148.0
30-31	16774.0
32-33	19928.0
34-35	26064.0
36-37	35351.0
38-39	39756.0
40-41	36472.0
42-43	38068.0
44-45	43297.0
46-47	47476.0
48-49	28842.0
50-51	28388.0
52-53	11088.0
54-55	10914.0
56-57	11447.0
58-59	12385.0
60-61	13013.0
62-63	56144.0
64-65	60030.0
66-67	63267.0
68-69	63035.0
70-71	63006.0
72-73	61098.0
74-75	62686.0
76-77	65501.0
78-79	68428.0
80-81	69202.0
82-83	68139.0
84-85	72302.0
86-87	88259.0
88-89	80247.0
90-91	83883.0
92-93	90370.0
94-95	112371.0
96-97	258115.0
98-99	468330.0
100-101	4621024.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.29223994100909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.2283766501928	76.10488110794977
2	7.573265485325527	12.91881553822695
3	1.9250353385016854	4.925717279592221
4	0.6282812365777528	2.1435005592249436
5	0.2918983901394214	1.2448333765082906
6	0.1301783500855391	0.6661921842372288
7	0.07446319255111385	0.4445792740090214
8	0.04405016582211482	0.3005709850192845
9	0.02590780712576373	0.19887614115444227
>10	0.07753799793826607	0.9600222272547779
>50	7.246980592235965E-4	0.04081947978791474
>100	2.5592826172484974E-4	0.039070193607386505
>500	2.47594190622113E-5	0.012121653427746648
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.265942859401775E-5	0.0	0.0	0.0
3	0.0	4.265942859401775E-5	0.0	0.0	0.0
4	0.0	9.953866671937476E-5	0.0	0.0	0.0
5	0.0	9.953866671937476E-5	0.0	0.0	0.0
6	0.0	9.953866671937476E-5	0.0	0.0	1.4219809531339252E-5
7	0.0	9.953866671937476E-5	0.0	0.0	2.8439619062678503E-5
8	0.0	1.4219809531339252E-4	0.0	0.0	2.8439619062678503E-5
9	0.0	2.4173676203276728E-4	0.0	1.4219809531339252E-5	8.53188571880355E-5
10-11	2.8439619062678503E-5	2.4173676203276728E-4	0.0	3.5549523828348124E-5	8.53188571880355E-5
12-13	2.8439619062678503E-5	2.4173676203276728E-4	0.0	4.265942859401775E-5	8.53188571880355E-5
14-15	2.8439619062678503E-5	3.1994571445513316E-4	0.0	4.265942859401775E-5	1.0664857148504439E-4
16-17	4.976933335968738E-5	3.3416552398647236E-4	0.0	4.265942859401775E-5	1.2797828578205327E-4
18-19	7.820895242236588E-5	3.6260514304915087E-4	0.0	7.820895242236588E-5	1.4219809531339252E-4
20-21	8.53188571880355E-5	3.910447621118294E-4	0.0	1.4219809531339252E-4	1.4219809531339252E-4
22-23	8.53188571880355E-5	5.545725717222308E-4	0.0	2.772862858611154E-4	1.4930800007906215E-4
24-25	1.2797828578205327E-4	6.25671619378927E-4	0.0	4.337041907058472E-4	1.635278096104014E-4
26-27	1.2797828578205327E-4	6.541112384416055E-4	0.0	9.456173338340603E-4	1.70637714376071E-4
28-29	1.2797828578205327E-4	6.541112384416055E-4	0.0	0.0041806240022137404	1.70637714376071E-4
30-31	1.70637714376071E-4	7.678697146923196E-4	0.0	0.013608357721491665	1.70637714376071E-4
32-33	1.7774761914174063E-4	7.678697146923196E-4	0.0	0.044017420404260654	1.70637714376071E-4
34-35	1.8485752390741026E-4	9.242876195370514E-4	0.0	0.0849918015688147	1.70637714376071E-4
36-37	1.8485752390741026E-4	0.0010949253339131225	0.0	0.1407263450268989	1.70637714376071E-4
38-39	1.8485752390741026E-4	0.0011162550482101312	0.0	0.20433155306057937	1.70637714376071E-4
40-41	1.8485752390741026E-4	0.001237123429226515	0.0	0.26457377614009814	1.70637714376071E-4
42-43	1.8485752390741026E-4	0.0012868927625862023	0.0	0.37541719143688757	1.7774761914174063E-4
44-45	1.8485752390741026E-4	0.0014930800007906214	0.0	0.4878958848297811	1.8485752390741026E-4
46-47	1.8485752390741026E-4	0.0016494979056353532	0.0	0.6132932751818962	1.919674286730799E-4
48-49	1.8485752390741026E-4	0.0016494979056353532	0.0	0.706248170088261	2.1329714297008877E-4
50-51	1.8485752390741026E-4	0.001841465334308433	0.0	1.048789161888692	2.1329714297008877E-4
52-53	1.9907733343874952E-4	0.0018556851438397723	0.0	1.4941749261245345	2.204070477357584E-4
54-55	1.9907733343874952E-4	0.0018699049533711116	0.0	2.0967322451102697	2.2751695250142803E-4
56-57	1.9907733343874952E-4	0.0019054544771994596	0.0	2.7507723845042187	2.2751695250142803E-4
58-59	1.9907733343874952E-4	0.0019125643819651293	0.0	3.430173554197311	2.2751695250142803E-4
60-61	1.9907733343874952E-4	0.0019694436200904863	0.0	4.096961752836106	2.2751695250142803E-4
62-63	1.9907733343874952E-4	0.002076092191575531	0.0	4.583300568522205	2.2751695250142803E-4
64-65	1.9907733343874952E-4	0.00247424685845303	0.0	4.599212535387773	2.2751695250142803E-4
66-67	1.9907733343874952E-4	0.002488466667984369	0.0	4.60596694491516	2.2751695250142803E-4
68-69	1.9907733343874952E-4	0.002552455810875396	0.0	4.612671585109186	2.2751695250142803E-4
70-71	1.9907733343874952E-4	0.0025666756204067347	0.0	4.618786103207662	2.4173676203276728E-4
72-73	1.9907733343874952E-4	0.0025737855251724046	0.0	4.62438870816301	2.4173676203276728E-4
74-75	1.9907733343874952E-4	0.0026519944775947706	0.0	4.629898884356404	2.4173676203276728E-4
76-77	1.9907733343874952E-4	0.002772862858611154	0.0	4.633859101310882	2.4884666679843694E-4
78-79	1.9907733343874952E-4	0.002829742096736511	0.0	4.636048951978708	2.5595657156410654E-4
80-81	1.9907733343874952E-4	0.002829742096736511	0.0	4.637207866455512	2.5595657156410654E-4
82-83	1.9907733343874952E-4	0.002829742096736511	0.0	4.638125044170284	2.701763810954458E-4
84-85	1.9907733343874952E-4	0.0028724015253305288	0.0	4.638537418646692	2.701763810954458E-4
86-87	1.9907733343874952E-4	0.002929280763455886	0.0	4.638708056361068	2.701763810954458E-4
88	1.9907733343874952E-4	0.0030856986683006175	0.0	4.638729386075365	2.701763810954458E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5925	0.0	39.891987	1
GTATCAA	9615	0.0	28.904612	1
TCAACGC	10310	0.0	25.398079	4
ATCAACG	10445	0.0	25.071774	3
CAACGCA	10610	0.0	24.801792	5
AACGCAG	11225	0.0	23.636402	6
TATCAAC	11600	0.0	23.21223	2
GTACATG	20955	0.0	20.245094	1
GTGTAGA	10890	0.0	20.181868	94
TACATGG	21045	0.0	19.829536	2
ACGCAGA	13240	0.0	19.779055	7
TGTAGAT	10595	0.0	19.56376	94
CGCAGAG	13550	0.0	19.35835	8
ACATGGG	21215	0.0	18.66879	3
GGTGTAG	11955	0.0	18.439026	94
AGGTGTA	14415	0.0	16.387873	94
CATGGGG	15620	0.0	16.265268	4
GTGTAGC	3940	0.0	15.491886	1
TATACCG	615	0.0	15.48255	5
GCAGAGT	17405	0.0	14.971551	9
>>END_MODULE