FastQCFastQC Report
Fri 27 May 2016
SRR2140331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23532032
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204050.036.8195231
GTATCAA324550.030.290351
TCAACGC334350.027.566894
ATCAACG339250.027.3239063
CAACGCA342900.026.8790135
AACGCAG360850.025.6739066
TATCAAC391550.024.2538952
ACGCAGA423900.021.7337687
CGCAGAG434100.021.1726598
GTACATG694950.019.3983861
TACATGG691300.018.8103392
ACATGGG695300.018.1627713
GCAGAGT552500.016.5652969
CATGGGG514250.016.0582834
GTGTAGA346750.015.83275894
AGAGTAC474800.015.41442610-11
GTACTAG58400.015.384211
CAGAGTA542550.014.91731710-11
TGTAGAT339550.014.063827594
GTGTAGC128100.013.7560771
Produced by FastQC (version 0.11.5)