##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140331_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23532032
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.786433785233676	33.0	31.0	34.0	30.0	34.0
2	31.822786999439742	33.0	31.0	34.0	30.0	34.0
3	32.04919439171254	34.0	31.0	34.0	30.0	34.0
4	35.499848589361086	37.0	35.0	37.0	33.0	37.0
5	35.58889742288299	37.0	35.0	37.0	33.0	37.0
6	35.64321551152064	37.0	35.0	37.0	33.0	37.0
7	35.540056124349995	37.0	35.0	37.0	33.0	37.0
8	35.51084262506527	37.0	35.0	37.0	33.0	37.0
9	37.17094299378821	39.0	37.0	39.0	34.0	39.0
10-11	37.180080347502496	39.0	37.0	39.0	34.0	39.0
12-13	37.112196494548364	39.0	37.0	39.0	33.5	39.0
14-15	38.456248147206324	40.0	38.0	41.0	34.0	41.0
16-17	38.4588149038723	40.0	38.0	41.0	33.5	41.0
18-19	38.46621258631639	40.0	38.0	41.0	33.5	41.0
20-21	38.530323910662666	40.0	38.0	41.0	34.0	41.0
22-23	38.48500209784841	40.0	38.0	41.0	34.0	41.0
24-25	38.46022416557349	40.0	38.0	41.0	33.5	41.0
26-27	38.41869449313283	40.0	38.0	41.0	33.0	41.0
28-29	38.342278256092186	40.0	38.0	41.0	33.0	41.0
30-31	38.22320360881905	40.0	38.0	41.0	33.0	41.0
32-33	38.1751736068389	40.0	38.0	41.0	33.0	41.0
34-35	38.0252093980099	40.0	38.0	41.0	32.5	41.0
36-37	37.89217159380535	40.0	38.0	41.0	32.0	41.0
38-39	37.87218829053603	40.0	37.0	41.0	32.0	41.0
40-41	37.785323247869634	40.0	37.0	41.0	32.0	41.0
42-43	37.58990553491812	40.0	37.0	41.0	31.0	41.0
44-45	37.63426269179903	40.0	37.0	41.0	31.5	41.0
46-47	37.84163938307682	40.0	37.0	41.0	32.0	41.0
48-49	37.82459470516383	40.0	37.0	41.0	32.0	41.0
50-51	37.724918520284824	40.0	37.0	41.0	32.0	41.0
52-53	37.65208236184134	40.0	37.0	41.0	32.0	41.0
54-55	37.576005988254856	40.0	36.0	41.0	32.0	41.0
56-57	37.64421224148887	40.0	36.0	41.0	32.0	41.0
58-59	37.46237706830512	39.5	36.0	41.0	31.0	41.0
60-61	37.39253696912169	39.0	36.0	41.0	31.0	41.0
62-63	37.16934500066155	39.0	35.0	41.0	31.0	41.0
64-65	36.96841640678658	39.0	35.0	41.0	31.0	41.0
66-67	36.70310005771432	38.0	35.0	40.0	31.0	41.0
68-69	36.481233578686755	37.5	35.0	40.0	31.0	41.0
70-71	36.198924399201786	37.0	35.0	40.0	30.5	41.0
72-73	35.87320016856272	36.5	34.0	39.0	30.0	41.0
74-75	35.53889133117192	36.0	34.0	39.0	30.0	41.0
76-77	35.211580164043795	36.0	34.0	39.0	30.0	40.0
78-79	34.84748849474386	35.0	34.0	37.5	30.0	39.5
80-81	34.53236559214724	35.0	34.0	37.0	29.5	39.0
82-83	34.216004293484794	35.0	34.0	36.5	29.0	39.0
84-85	33.99332206279958	35.0	33.5	36.0	29.0	37.5
86-87	33.749346012224805	35.0	33.0	36.0	29.0	37.0
88-89	33.496450275398885	35.0	33.0	35.0	29.0	37.0
90-91	33.31599606620038	35.0	33.0	35.0	29.0	36.0
92-93	33.21851874806877	35.0	33.0	35.0	29.0	36.0
94-95	33.202689573974254	35.0	33.0	35.0	29.0	36.0
96-97	33.18260015780096	35.0	33.0	35.0	29.0	36.0
98-99	33.24787673481532	35.0	33.0	35.0	29.0	35.0
100	32.59169732848401	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	5.0
15	20.0
16	122.0
17	847.0
18	4623.0
19	15264.0
20	33681.0
21	59696.0
22	89049.0
23	119060.0
24	156038.0
25	194577.0
26	223091.0
27	238353.0
28	270396.0
29	324027.0
30	384245.0
31	469876.0
32	606701.0
33	810950.0
34	1139279.0
35	1658799.0
36	2620951.0
37	4441399.0
38	6093036.0
39	3399293.0
40	178649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61071734051696	20.972995447226996	14.796669492885272	23.61961771937077
2	14.078818618838188	21.267882134762914	42.077136119445896	22.576163126953002
3	17.63338739122086	26.586301778334214	30.50086322901116	25.279447601433763
4	12.119604305693128	18.787995797184397	39.70332701369757	29.389072883424905
5	13.220977219312612	38.46906277110468	35.55891279885874	12.751047210723963
6	29.02871639604697	38.57814831929289	19.540453175385796	12.85268210927434
7	26.09912215055902	33.1510957500562	23.554021716542643	17.19576038284214
8	23.76497182536529	37.38060257466735	21.585913830114116	17.268511769853248
9	24.748288763063016	18.371218785554266	21.78664661476222	35.093845836620496
10-11	23.455097703633044	27.72877241814946	29.245302823955573	19.570827054261926
12-13	24.18409586977918	25.134263700201288	29.17099536034869	21.510645069670844
14-15	21.624250303998505	26.685698193840796	26.58632058465797	25.10373091750273
16-17	21.504575279473276	29.634025451033907	27.307253601821298	21.554145667671527
18-19	21.494112905336973	29.27206827547496	28.421614538091195	20.81220428109687
20-21	22.453227434830524	28.29686534435989	28.171542477561047	21.07836474324854
22-23	22.214301272660464	28.158703314099192	27.941894717238057	21.685100696002284
24-25	21.94404617704365	28.34510557554743	27.97625762839826	21.734590619010657
26-27	21.92051343346647	28.486451623991176	27.862131840366537	21.730903102175816
28-29	21.797233186197577	28.57413599275774	27.929818820261332	21.698812000783356
30-31	22.10862591658974	28.297072357527213	27.920034654441235	21.674267071441808
32-33	21.86481524970512	28.389814155217298	27.90461586284545	21.840754732232135
34-35	22.090506782661762	28.41268962883663	27.92697119274765	21.569832395753952
36-37	22.04461412053011	28.23076985904882	27.936786731399444	21.787829289021623
38-39	21.815710136585484	28.24741537107789	28.159798226919357	21.777076265417268
40-41	22.00083593633872	28.084810355695218	28.139242762227294	21.775110945738767
42-43	21.83033284065909	28.14016302784561	28.05594254031642	21.97356159117888
44-45	21.895515745758264	28.20477873319624	28.054877211298624	21.844828309746873
46-47	21.820345193573385	28.13935883699178	27.994745037704817	22.04555093173002
48-49	21.709771212371294	28.10903573527671	28.17363563420165	22.007557418150345
50-51	21.62584882474088	28.132242223467074	28.197836796157873	22.044072155634176
52-53	21.65656393942524	28.01502005742222	28.181752428069895	22.146663575082645
54-55	21.72865601742716	27.84833494135833	28.225435963871497	22.197573077343005
56-57	21.628279130912084	27.73901890422309	28.285651473757966	22.347050491106856
58-59	21.55833140338912	27.709225265208953	28.42401081527025	22.308432516131678
60-61	21.514617703355697	27.58048672023606	28.649914601888682	22.254980974519565
62-63	21.460771707132235	27.724066711234173	28.618677765214994	22.1964838164186
64-65	21.309117654148324	27.956085381953876	28.36276395664756	22.37203300725024
66-67	21.18948376626052	28.139173786832828	28.162024236851817	22.509318210054833
68-69	21.244041639186086	28.240219001149825	28.164069768268806	22.35166959139528
70-71	21.24731866285764	28.345991282874216	27.86108135596761	22.54560869830053
72-73	21.508246950080363	28.188631664237047	27.621827508888686	22.681293876793905
74-75	21.722179248432116	28.081814050003484	27.60150860633664	22.594498095227763
76-77	21.847715227412333	27.708888687736373	27.724325261039763	22.71907082381153
78-79	22.12762611655423	27.443269263344945	27.689857057167146	22.739247562933677
80-81	22.351496401328212	27.505528581217188	27.400356283730154	22.74261873372445
82-83	22.16343704590629	27.540049526073567	27.569052839799333	22.727460588220808
84-85	22.2527533500258	27.67469176999829	27.49935334942651	22.573201530549408
86-87	22.01287380742414	27.90932230866411	27.69391019752183	22.383893686389918
88-89	21.878353447977474	27.99624955797913	27.647644134149385	22.477752859894007
90-91	21.73331248158184	28.161617563983494	27.899183377324142	22.205886577110522
92-93	21.649633554156015	27.842375461238895	28.361659221729475	22.14633176287562
94-95	21.33142534738472	27.82939947929683	28.734174650189175	22.105000523129277
96-97	21.679656946065098	27.761553930880144	28.68857686491767	21.870212258137087
98-99	22.998038389461264	29.64285102246328	29.064022174544917	18.295088413530543
100	22.203947992948862	30.87860067675315	22.756999843793967	24.16045148650402
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10399.0
1	7948.5
2	5813.5
3	5969.0
4	5705.5
5	6029.5
6	7283.0
7	8652.5
8	9658.5
9	10827.5
10	11921.0
11	12625.5
12	13453.5
13	14681.5
14	16535.0
15	19297.5
16	23008.0
17	27601.5
18	33310.5
19	40390.5
20	49603.0
21	62199.5
22	78627.5
23	99933.5
24	127616.5
25	163308.0
26	207858.5
27	257390.5
28	311414.0
29	372362.5
30	430154.5
31	487083.0
32	546432.0
33	601752.0
34	655454.5
35	704769.0
36	755507.5
37	806097.0
38	844550.5
39	870814.5
40	898213.5
41	923329.0
42	939109.0
43	955928.5
44	969685.5
45	980928.0
46	986291.5
47	983299.5
48	974385.0
49	951625.5
50	922580.5
51	887466.0
52	841833.5
53	791132.0
54	735243.5
55	671490.0
56	605990.0
57	539198.0
58	471109.5
59	407863.0
60	342160.0
61	278722.5
62	224811.5
63	177115.5
64	136094.5
65	104717.0
66	81336.0
67	64122.5
68	51289.5
69	40062.0
70	31071.5
71	24456.5
72	19504.5
73	15394.5
74	12151.5
75	9693.0
76	7443.0
77	5696.0
78	4397.5
79	3273.0
80	2422.0
81	1763.0
82	1361.5
83	957.5
84	680.0
85	548.5
86	461.5
87	377.5
88	260.5
89	164.0
90	114.0
91	99.5
92	87.0
93	60.0
94	38.5
95	33.5
96	33.0
97	29.0
98	21.0
99	32.0
100	94.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02179582281717108
3	0.01246811155109767
4	0.004844460520876395
5	0.00911523492743848
6	0.00860529171471465
7	0.0028131867235264684
8	0.008507552598942583
9	0.004674479449968451
10-11	0.003480362426840147
12-13	0.00863078887535084
14-15	0.004578865097582733
16-17	0.0035972244130893583
18-19	0.005269413198146254
20-21	0.004092641239706742
22-23	0.009672737625183331
24-25	0.009038070529533183
26-27	0.022075071188107406
28-29	0.018698663124564967
30-31	0.015174840051049215
32-33	0.01187027469594045
34-35	0.008465446168859338
36-37	0.009709706705013615
38-39	0.005007803538629977
40-41	0.00604019061954483
42-43	0.0038485965185766167
44-45	0.0066946982661981805
46-47	0.009451969056202806
48-49	0.00688535895098106
50-51	0.009354818091117621
52-53	0.00101666408515918
54-55	0.0028027809945211676
56-57	5.473024906996148E-4
58-59	0.002542224300157793
60-61	9.123766939793952E-4
62-63	0.006691010124771397
64-65	0.003472996913114602
66-67	0.0035305552757911437
68-69	0.004819957169776926
70-71	7.135706514400079E-4
72-73	5.381735891477082E-4
74-75	0.0
76-77	7.260852390670744E-6
78-79	5.311884352297584E-4
80-81	1.337752971873967E-4
82-83	0.0
84-85	8.938502543841959E-4
86-87	0.0034477530390474268
88-89	0.021467406804008334
90-91	0.0027074627940622416
92-93	0.0011657795607142457
94-95	0.001055896689524027
96-97	3.5399075155266715E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	9758.0
20-21	18120.0
22-23	20239.0
24-25	23320.0
26-27	28183.0
28-29	35794.0
30-31	48058.0
32-33	55077.0
34-35	68599.0
36-37	94238.0
38-39	107962.0
40-41	97175.0
42-43	101600.0
44-45	116377.0
46-47	138829.0
48-49	90590.0
50-51	90451.0
52-53	41884.0
54-55	43117.0
56-57	46506.0
58-59	48789.0
60-61	50493.0
62-63	182007.0
64-65	198394.0
66-67	208922.0
68-69	212318.0
70-71	213234.0
72-73	209730.0
74-75	217030.0
76-77	229696.0
78-79	240747.0
80-81	246604.0
82-83	244662.0
84-85	262140.0
86-87	317488.0
88-89	296990.0
90-91	310268.0
92-93	336336.0
94-95	418295.0
96-97	901176.0
98-99	1636138.0
100-101	1.5274698E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.82721410139497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.0028376757436	69.45194150570498
2	8.11656544189489	12.958456145962534
3	2.459250684518804	5.88945392766254
4	1.0519156994824568	3.358859990368189
5	0.5110760553931458	2.039888884798253
6	0.3051133451699615	1.4613808998045206
7	0.16935619396381618	0.9463463208462851
8	0.10246109156853657	0.6543346794963362
9	0.07155995025814516	0.5141188323307914
>10	0.20592861183244973	2.434002229147222
>50	0.003174928589336896	0.16943691263085472
>100	7.080523715423579E-4	0.09036310229349986
>500	4.189251720051675E-5	0.02064811343181603
>1k	1.0376695990169592E-5	0.01076845552214061
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2748580318095777E-5	0.0
2	0.0	2.5497160636191553E-5	0.0	2.1247633863492962E-5	4.249526772698592E-6
3	0.0	2.5497160636191553E-5	0.0	2.9746687408890145E-5	4.249526772698592E-6
4	0.0	6.799242836317747E-5	0.0	5.099432127238311E-5	4.249526772698592E-6
5	0.0	6.799242836317747E-5	0.0	5.52438480450817E-5	4.249526772698592E-6
6	0.0	6.799242836317747E-5	0.0	6.799242836317747E-5	4.249526772698592E-6
7	0.0	7.224195513587607E-5	0.0	7.649148190857466E-5	4.249526772698592E-6
8	0.0	9.348958899936902E-5	0.0	8.074100868127325E-5	8.499053545397184E-6
9	0.0	1.8697917799873805E-4	0.0	1.3173532995365637E-4	1.2748580318095777E-5
10-11	0.0	1.8697917799873805E-4	0.0	1.4873343704445072E-4	1.6998107090794368E-5
12-13	2.124763386349296E-6	1.8697917799873805E-4	0.0	1.9547823154413523E-4	1.6998107090794368E-5
14-15	4.249526772698592E-6	2.188506287939775E-4	0.0	2.4647255281651833E-4	3.6120977567938036E-5
16-17	4.249526772698592E-6	2.2522491895302538E-4	0.0	3.0596592763429864E-4	4.2495267726985925E-5
18-19	4.249526772698592E-6	2.5284684297556623E-4	0.0	3.5271072213398315E-4	5.5243848045081695E-5
20-21	4.249526772698592E-6	2.847182937708057E-4	0.0	4.2495267726985926E-4	6.161813820412958E-5
22-23	4.249526772698592E-6	3.2296403472509305E-4	0.0	6.586766497682818E-4	6.799242836317748E-5
24-25	4.249526772698592E-6	3.5058595874763385E-4	0.0	0.001204740840060051	9.136482561301974E-5
26-27	4.249526772698592E-6	3.6120977567938035E-4	0.0	0.002381859756097561	9.773911577206763E-5
28-29	4.249526772698592E-6	3.6120977567938035E-4	0.0	0.00875614991514545	1.0623816931746482E-4
30-31	4.249526772698592E-6	4.1220409695176346E-4	0.0	0.030507352701203194	1.104876960901634E-4
32-33	4.249526772698592E-6	4.270774406562085E-4	0.0	0.0932941107678249	1.2748580318095777E-4
34-35	4.249526772698592E-6	4.993193957920846E-4	0.0	0.17607064277322076	1.2748580318095777E-4
36-37	4.249526772698592E-6	5.779356410870086E-4	0.0	0.29559070801875503	1.2748580318095777E-4
38-39	4.249526772698592E-6	6.055575651095494E-4	0.0	0.4357783467233089	1.3173532995365637E-4
40-41	4.249526772698592E-6	6.969223907225691E-4	0.0	0.5630814202530406	1.4023438349905354E-4
42-43	4.249526772698592E-6	7.160452611997128E-4	0.0	0.7586552661495616	1.4235914688540284E-4
44-45	4.249526772698592E-6	8.116596135854311E-4	0.0	0.94572155944714	1.4660867365810142E-4
46-47	4.249526772698592E-6	8.881510954940058E-4	0.0	1.155061747323818	1.5510772720349862E-4
48-49	4.249526772698592E-6	8.902758588803551E-4	0.0	1.2963670115695916	1.657315441352451E-4
50-51	4.249526772698592E-6	9.922645014251211E-4	0.0	1.638787504623485	1.657315441352451E-4
52-53	4.249526772698592E-6	9.986387915841691E-4	0.0	2.066901829812232	1.7635536106699157E-4
54-55	4.249526772698592E-6	0.0010071378451295663	0.0	2.6211803553556274	1.9122870477143665E-4
56-57	4.249526772698592E-6	0.0010198864254476621	0.0	3.2245812856280325	2.0185252170318313E-4
58-59	4.249526772698592E-6	0.0010283854789930593	0.0	3.85215139942016	2.0610204847588173E-4
60-61	4.249526772698592E-6	0.0010411340593111551	0.0	4.4686174997552275	2.0822681186223103E-4
62-63	4.249526772698592E-6	0.0010623816931746482	0.0	4.923988289664063	2.209753921803268E-4
64-65	4.249526772698592E-6	0.0011516217554013186	0.0	4.947651779497836	2.2310015556667608E-4
66-67	4.249526772698592E-6	0.0011558712821740172	0.0	4.9548483530874	2.3797349927112116E-4
68-69	4.249526772698592E-6	0.0011877427329692566	0.0	4.960974045930245	2.5284684297556623E-4
70-71	4.249526772698592E-6	0.0012068656034464002	0.0	4.9663603211146405	2.6347065990731274E-4
72-73	4.249526772698592E-6	0.0012217389471508452	0.0	4.97158723904506	3.0171640086160004E-4
74-75	4.249526772698592E-6	0.0012599846881051328	0.0	4.976257468968256	3.1234021779334654E-4
76-77	4.249526772698592E-6	0.0013003551924457694	0.0	4.979678338020278	3.1658974456604514E-4
78-79	4.249526772698592E-6	0.0013237275896956116	0.0	4.981662867023129	3.2083927133874375E-4
80-81	4.249526772698592E-6	0.00132797711646831	0.0	4.9827061258458265	3.335878516568395E-4
82-83	4.249526772698592E-6	0.0013364761700137072	0.0	4.983602775994865	3.44211668588586E-4
84-85	4.249526772698592E-6	0.001355599040490851	0.0	4.983987358167795	3.5271072213398315E-4
86-87	4.249526772698592E-6	0.0013895952546724396	0.0	4.984119093497748	3.5271072213398315E-4
88	4.249526772698592E-6	0.0014660867365810144	0.0	4.98412971731468	3.5271072213398315E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20405	0.0	36.819523	1
GTATCAA	32455	0.0	30.29035	1
TCAACGC	33435	0.0	27.56689	4
ATCAACG	33925	0.0	27.323906	3
CAACGCA	34290	0.0	26.879013	5
AACGCAG	36085	0.0	25.673906	6
TATCAAC	39155	0.0	24.253895	2
ACGCAGA	42390	0.0	21.733768	7
CGCAGAG	43410	0.0	21.172659	8
GTACATG	69495	0.0	19.398386	1
TACATGG	69130	0.0	18.810339	2
ACATGGG	69530	0.0	18.162771	3
GCAGAGT	55250	0.0	16.565296	9
CATGGGG	51425	0.0	16.058283	4
GTGTAGA	34675	0.0	15.832758	94
AGAGTAC	47480	0.0	15.414426	10-11
GTACTAG	5840	0.0	15.38421	1
CAGAGTA	54255	0.0	14.917317	10-11
TGTAGAT	33955	0.0	14.0638275	94
GTGTAGC	12810	0.0	13.756077	1
>>END_MODULE