FastQCFastQC Report
Fri 27 May 2016
SRR2140330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7261818
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63950.034.907781
GTATCAA103550.029.9191861
ATCAACG107750.026.74183
TCAACGC111750.025.9735764
CAACGCA111150.025.9607145
AACGCAG116800.024.8504036
TATCAAC122150.024.425582
ACGCAGA133950.021.5750357
CGCAGAG135700.021.2965098
GTACATG211900.018.666461
TACATGG211300.018.0970732
ACATGGG216550.017.1273983
GCAGAGT173000.016.8029549
CATGGGG150050.015.8088034
CTCGATA8550.015.15170394
AGAGTAC152150.015.13083710-11
CAGAGTA172300.014.67898810-11
TATACCG6400.014.5867955
GTGTAGC42600.013.1504721
GTATAAT44500.013.0658471
Produced by FastQC (version 0.11.5)