FastQCFastQC Report
Fri 27 May 2016
SRR2140329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9326381
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83800.039.740441
GTATCAA142400.032.790711
TCAACGC149950.029.313284
ATCAACG151600.029.0256273
CAACGCA154500.028.506265
AACGCAG160400.027.6782656
TATCAAC170200.026.4777012
TCGGTGG59550.025.88720594
ACGCAGA191950.022.9919767
CGCAGAG197250.022.3960428
GTACATG318600.020.670471
TACATGG316250.020.239482
ACATGGG323650.019.3798183
CTCGGTG82500.018.36368894
CATGGGG229950.017.7935524
GCAGAGT246650.017.642539
TCTCGGT85350.016.59946392-93
AGAGTAC213600.016.51594710-11
CAGAGTA238600.015.86527710-11
GTGGTAT55400.015.4257711
Produced by FastQC (version 0.11.5)