##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140329_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9326381
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94794272290613	33.0	31.0	34.0	30.0	34.0
2	31.981515659718383	34.0	31.0	34.0	30.0	34.0
3	32.19080605864161	34.0	31.0	34.0	30.0	34.0
4	35.62049909820326	37.0	35.0	37.0	35.0	37.0
5	35.697300592802286	37.0	35.0	37.0	35.0	37.0
6	35.75475631973431	37.0	35.0	37.0	35.0	37.0
7	35.65639522983245	37.0	35.0	37.0	33.0	37.0
8	35.6181879123317	37.0	35.0	37.0	33.0	37.0
9	37.30965387324408	39.0	38.0	39.0	34.0	39.0
10-11	37.31018387518159	39.0	38.0	39.0	34.0	39.0
12-13	37.24502183644438	39.0	37.5	39.0	34.0	39.0
14-15	38.626040047045045	41.0	38.0	41.0	34.0	41.0
16-17	38.63746151910371	40.5	38.0	41.0	34.0	41.0
18-19	38.650344544148474	40.0	38.5	41.0	34.0	41.0
20-21	38.702545980630696	40.0	39.0	41.0	34.0	41.0
22-23	38.663892649465424	40.0	38.0	41.0	34.0	41.0
24-25	38.62984616817211	40.0	38.0	41.0	34.0	41.0
26-27	38.58560585307801	40.0	38.0	41.0	34.0	41.0
28-29	38.51468357681425	40.0	38.0	41.0	34.0	41.0
30-31	38.39246776599396	40.0	38.0	41.0	33.0	41.0
32-33	38.34018858790252	40.0	38.0	41.0	33.0	41.0
34-35	38.187312233393115	40.0	38.0	41.0	33.0	41.0
36-37	38.06790096194024	40.0	38.0	41.0	33.0	41.0
38-39	38.04935435609463	40.0	38.0	41.0	33.0	41.0
40-41	37.961072336972705	40.0	38.0	41.0	33.0	41.0
42-43	37.77651348324045	40.0	37.0	41.0	32.0	41.0
44-45	37.837304150839486	40.0	37.5	41.0	32.5	41.0
46-47	38.054006824917224	40.0	38.0	41.0	33.0	41.0
48-49	38.049708882652	40.0	37.5	41.0	33.0	41.0
50-51	37.959399957735485	40.0	37.0	41.0	33.0	41.0
52-53	37.885997024522226	40.0	37.0	41.0	32.5	41.0
54-55	37.80757070596874	40.0	37.0	41.0	32.0	41.0
56-57	37.864582340673905	40.0	37.0	41.0	33.0	41.0
58-59	37.67708573270785	40.0	36.0	41.0	32.0	41.0
60-61	37.59623053104201	39.0	36.0	41.0	32.0	41.0
62-63	37.368260548854536	39.0	35.5	41.0	32.0	41.0
64-65	37.153906530129404	39.0	35.0	41.0	31.5	41.0
66-67	36.871081439338774	38.5	35.0	40.5	31.0	41.0
68-69	36.63146692651275	38.0	35.0	40.0	31.0	41.0
70-71	36.32368844079606	37.0	35.0	40.0	31.0	41.0
72-73	35.97360936485458	36.5	35.0	39.0	31.0	41.0
74-75	35.620710519424335	36.0	34.0	39.0	30.0	41.0
76-77	35.26045709592482	35.5	34.0	38.5	30.0	40.0
78-79	34.88122110168388	35.0	34.0	37.0	30.0	39.5
80-81	34.54345764679179	35.0	34.0	37.0	29.5	39.0
82-83	34.220248833558315	35.0	34.0	36.5	29.0	39.0
84-85	33.9908040061667	35.0	34.0	36.0	29.0	37.0
86-87	33.75594617414302	35.0	33.0	36.0	29.0	37.0
88-89	33.50647409414891	35.0	33.0	35.0	29.0	37.0
90-91	33.310600262841646	35.0	33.0	35.0	29.0	36.0
92-93	33.20736314015309	35.0	33.0	35.0	29.0	36.0
94-95	33.19191960578007	35.0	33.0	35.0	29.0	36.0
96-97	33.16677586776594	35.0	33.0	35.0	29.0	36.0
98-99	33.26639089498523	35.0	33.0	35.0	29.0	35.0
100	32.63107235544685	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	0.0
15	13.0
16	49.0
17	280.0
18	1515.0
19	4652.0
20	10302.0
21	18599.0
22	28448.0
23	40330.0
24	53785.0
25	68680.0
26	80765.0
27	87381.0
28	99750.0
29	121054.0
30	143024.0
31	174981.0
32	227043.0
33	306639.0
34	436133.0
35	651683.0
36	1056807.0
37	1835101.0
38	2474811.0
39	1322107.0
40	82446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.86234521193162	20.777544901929268	14.731748574286211	23.628361311852906
2	14.210678549286012	21.197934133313115	41.8187215602555	22.772665757145376
3	17.78595777371995	26.44121491169937	30.432995023800057	25.339832290780624
4	12.253949313762368	18.554568962818056	39.45525397805086	29.736227745368716
5	13.286484647874577	38.42911531455096	35.41242470632087	12.871975331253585
6	29.287845069792734	38.315653751348044	19.393126897410028	13.003374281449195
7	26.31958808208764	33.059597067593955	23.299460762395547	17.321354087922856
8	24.139592395130325	36.983573410300366	21.45860335759249	17.418230836976818
9	25.00481187163524	18.077325141946194	21.596065920979363	35.321797065439206
10-11	23.650460345836258	27.539676037997722	29.110145665380514	19.6997179507855
12-13	24.346036705103216	25.01765983051388	29.04624047340946	21.590062990973447
14-15	21.783589063378766	26.511714936282395	26.53573938857474	25.1689566117641
16-17	21.584576992530916	29.561595322173684	27.18533512773534	21.66849255756006
18-19	21.655280308154133	29.131433292559585	28.280862258973105	20.932424140313177
20-21	22.672016542494962	28.185990204446664	27.967799253381948	21.174193999676426
22-23	22.44036290635155	28.042323327304363	27.72672574527712	21.790588021066966
24-25	22.09255867214754	28.203337145018363	27.82325385741719	21.8808503254169
26-27	22.059734227598724	28.35779356622188	27.712645321845674	21.869826884333726
28-29	21.967145648162663	28.394130976357307	27.827565800014813	21.811157575465213
30-31	22.209868082263476	28.208529112034782	27.75484066529254	21.826762140409205
32-33	21.995014486135716	28.26099247701607	27.745554864278105	21.998438172570104
34-35	22.183876377892993	28.221475128615246	27.78379839270228	21.810850100789477
36-37	22.1244917506544	28.033123140394174	27.826289168604077	22.01609594034734
38-39	21.9366877502405	28.027001338348285	28.058776443222165	21.97753446818905
40-41	22.07262756961227	27.87620698122432	28.0672719073171	21.983893541846307
42-43	21.9069616998146	27.875829280964304	28.04637178790857	22.170837231312532
44-45	21.917894615695314	27.859431361832048	28.098225565699668	22.12444845677297
46-47	21.816468931624886	27.750990857362172	28.111040667429926	22.321499543583016
48-49	21.600841790031673	27.696105634795394	28.296340244429203	22.406712330743733
50-51	21.492178060322498	27.703185683886417	28.416964743187467	22.387671512603617
52-53	21.448854028578797	27.674073198395842	28.436008654464935	22.441064118560426
54-55	21.456047619528007	27.578983624199637	28.471557604273972	22.49341115199838
56-57	21.40583276090937	27.69771266010385	28.327167351734833	22.569287227251944
58-59	21.389265273733887	27.705903714994477	28.295088668709518	22.609742342562114
60-61	21.3753470663541	27.487256222255475	28.4159088012148	22.721487910175625
62-63	21.402960217834497	27.483221679657614	28.275413770077567	22.83840433243032
64-65	21.468076351963976	27.552527959607136	28.181457651787795	22.797938036641096
66-67	21.405356837115587	27.67909856570812	27.87535279995657	23.040191797219723
68-69	21.42486368782135	27.73901269025757	27.77818613122299	23.057937490698087
70-71	21.550166721128882	27.699759382303522	27.613851206002916	23.136222690564676
72-73	21.72160746433972	27.675233115336738	27.2197705515155	23.383388868808048
74-75	21.80778258608932	27.661434480545356	27.02025041082393	23.510532522541393
76-77	22.072493553533885	27.421442619259693	27.120402898422984	23.385660928783434
78-79	22.175575988071245	27.164068075012455	27.251494018974782	23.408861917941515
80-81	22.263287398436567	27.118624509928065	27.264145242578586	23.35394284905678
82-83	22.22863104914458	27.2176659884806	27.207110595179014	23.346592367195807
84-85	22.362321249410524	27.00859171965952	27.360691251313174	23.268395779616778
86-87	22.11316966526214	27.191652191699657	27.603509990163815	23.091668152874384
88-89	22.290494751480914	27.375488095472615	27.35272414795175	22.98129300509472
90-91	22.190937929628618	27.61703519145568	27.602139434244204	22.589887444671497
92-93	21.898943459145062	27.555625417586942	27.95128925484134	22.594141868426657
94-95	21.79467566867052	27.5619763777842	28.17799409274175	22.465353860803532
96-97	21.9155833679164	27.497605841718148	28.399480285968988	22.187330504396467
98-99	23.30183951924221	29.47006641209107	28.586171650844626	18.64192241782209
100	22.826753027901223	30.583538785308207	22.209796651230178	24.379911535560396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4066.0
1	3115.5
2	2357.5
3	2479.5
4	2348.5
5	2412.5
6	2858.0
7	3386.0
8	3784.0
9	4237.5
10	4609.0
11	4812.5
12	5167.0
13	5752.5
14	6613.5
15	7788.5
16	9075.0
17	10592.0
18	12353.5
19	14555.5
20	17668.5
21	21956.5
22	27480.5
23	34362.0
24	43977.5
25	57580.0
26	74586.0
27	93637.5
28	113704.0
29	134955.0
30	155387.0
31	175323.5
32	196589.0
33	217109.0
34	237076.0
35	255231.5
36	273831.5
37	293712.0
38	309827.5
39	320507.5
40	332789.0
41	345565.0
42	354090.5
43	363832.0
44	373625.5
45	380757.5
46	384376.0
47	385201.5
48	383581.0
49	379018.5
50	370718.5
51	358690.0
52	343505.5
53	324133.0
54	302110.5
55	276803.0
56	248777.0
57	220250.0
58	193168.0
59	167902.0
60	141514.0
61	115587.5
62	92433.0
63	72302.0
64	54856.0
65	41083.0
66	31354.5
67	24715.0
68	19541.0
69	14877.5
70	11432.0
71	8993.0
72	7176.0
73	5623.0
74	4445.5
75	3527.5
76	2706.0
77	2083.5
78	1601.0
79	1196.5
80	921.0
81	715.0
82	550.5
83	402.0
84	292.0
85	223.0
86	189.0
87	154.0
88	103.0
89	75.0
90	58.0
91	47.5
92	44.0
93	29.0
94	14.0
95	12.0
96	13.0
97	12.5
98	10.5
99	13.5
100	37.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.022109326222036176
3	0.012791671281711523
4	0.00490007860498086
5	0.009617878574765495
6	0.009006709033225214
7	0.0026912904373089627
8	0.008899486306639198
9	0.005232469057397505
10-11	0.003742073157851904
12-13	0.008990625624237312
14-15	0.004996579058908273
16-17	0.0036455727039244913
18-19	0.0056667211000708635
20-21	0.004731046446324347
22-23	0.009828409708021082
24-25	0.009502790780026025
26-27	0.022566302021321257
28-29	0.01899083315498027
30-31	0.01573045228500453
32-33	0.01247275903531254
34-35	0.008892636826388845
36-37	0.009886203510317694
38-39	0.005136149820348893
40-41	0.005854925415119118
42-43	0.00377949508130393
44-45	0.006946456820222744
46-47	0.009503789355273885
48-49	0.006868506771815532
50-51	0.009687052169583743
52-53	0.0010456662828704508
54-55	0.0025578006442460374
56-57	5.797829679090127E-4
58-59	0.002655136735393222
60-61	9.809204325559844E-4
62-63	0.006725371589275836
64-65	0.0034557225759336324
66-67	0.0038541701641520982
68-69	0.004877211638850227
70-71	7.998085062799352E-4
72-73	5.927364678554945E-4
74-75	0.0
76-77	2.3725860493956994E-5
78-79	4.6793328207264207E-4
80-81	1.336109444854421E-4
82-83	0.0
84-85	8.288585359092678E-4
86-87	0.003347810769078202
88-89	0.02113072620635496
90-91	0.0026463177928975974
92-93	0.0012214581767714299
94-95	0.0012543529113735587
96-97	3.998431772969886E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3444.0
20-21	6354.0
22-23	6884.0
24-25	8110.0
26-27	9971.0
28-29	12507.0
30-31	15242.0
32-33	15630.0
34-35	18356.0
36-37	23665.0
38-39	27468.0
40-41	20894.0
42-43	13144.0
44-45	13375.0
46-47	13796.0
48-49	14062.0
50-51	14636.0
52-53	14701.0
54-55	15167.0
56-57	15782.0
58-59	16622.0
60-61	17689.0
62-63	47665.0
64-65	79574.0
66-67	84710.0
68-69	85710.0
70-71	86010.0
72-73	84353.0
74-75	88317.0
76-77	93361.0
78-79	100298.0
80-81	104401.0
82-83	104417.0
84-85	112063.0
86-87	137386.0
88-89	132790.0
90-91	140102.0
92-93	151682.0
94-95	182303.0
96-97	372878.0
98-99	658838.0
100-101	6162024.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.11628728577284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.56164621472385	74.49476875498688
2	7.93065579098516	13.341946417581715
3	2.0074553236763575	5.065790661591437
4	0.7450289409592212	2.5067627373576378
5	0.3110102559565079	1.3080514019429679
6	0.16792924853704352	0.8475350948175541
7	0.09510179601036681	0.5599726996220685
8	0.05134441420108913	0.3455121196366822
9	0.033811229151826806	0.25596675583381456
>10	0.09485245420296566	1.1372335925813155
>50	8.660436486133436E-4	0.04780697481252004
>100	2.3897821020191088E-4	0.03944546883950159
>500	3.9539843981611597E-5	0.026840374091354577
>1k	1.976989283676009E-5	0.02236694630451574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.2166817975804335E-5	0.0
2	0.0	3.2166817975804335E-5	0.0	3.2166817975804335E-5	0.0
3	0.0	3.2166817975804335E-5	0.0	5.361136329300722E-5	0.0
4	0.0	6.433363595160867E-5	0.0	6.433363595160867E-5	0.0
5	0.0	6.433363595160867E-5	0.0	7.505590861021011E-5	0.0
6	0.0	6.433363595160867E-5	0.0	8.577818126881156E-5	0.0
7	0.0	6.433363595160867E-5	0.0	8.577818126881156E-5	0.0
8	0.0	1.0722272658601444E-4	0.0	9.6500453927413E-5	0.0
9	0.0	1.6083408987902168E-4	0.0	1.715563625376231E-4	0.0
10-11	0.0	1.6083408987902168E-4	0.0	2.3588999848923178E-4	0.0
12-13	0.0	1.6083408987902168E-4	0.0	2.841402254529383E-4	0.0
14-15	0.0	2.626956801357354E-4	0.0	3.0022363444084043E-4	0.0
16-17	0.0	2.7877908912363756E-4	0.0	3.752795430510506E-4	0.0
18-19	0.0	3.0022363444084043E-4	0.0	4.5033545166126064E-4	1.6083408987902168E-5
20-21	0.0	3.2166817975804335E-4	0.0	5.79002723564478E-4	2.144454531720289E-5
22-23	0.0	3.752795430510506E-4	0.0	0.0010293381752257387	2.144454531720289E-5
24-25	0.0	4.074463610268549E-4	0.0	0.0019246479422189594	4.288909063440578E-5
26-27	0.0	4.1816863368545633E-4	0.0	0.00400476883798764	5.8972499622307946E-5
28-29	0.0	4.2352977001475705E-4	0.0	0.016447966258294616	7.505590861021011E-5
30-31	0.0	4.8250226963706506E-4	0.0	0.05701032372578388	8.041704493951083E-5
32-33	0.0	4.932245422956665E-4	0.0	0.16137020351195175	1.0722272658601444E-4
34-35	0.0	5.575581782472751E-4	0.0	0.3133691407202858	1.0722272658601444E-4
36-37	0.0	6.218918141988838E-4	0.0	0.5489481933024182	1.0722272658601444E-4
38-39	0.0	6.218918141988838E-4	0.0	0.8465341486692426	1.447506808911195E-4
40-41	0.0	6.647809048332896E-4	0.0	1.1426994029088025	1.5011181722042021E-4
42-43	0.0	6.808643138211917E-4	0.0	1.5533356400515914	1.5547295354972093E-4
44-45	0.0	7.827259040779054E-4	0.0	2.0819597655296302	1.661952262083224E-4
46-47	0.0	8.68504085346717E-4	0.0	2.6746173033248377	1.715563625376231E-4
48-49	0.0	8.68504085346717E-4	0.0	3.2698106586038036	1.715563625376231E-4
50-51	0.0	0.0010293381752257387	0.0	3.8581900096082284	1.715563625376231E-4
52-53	0.0	0.00104006044788434	0.0	4.4248192305246805	1.715563625376231E-4
54-55	0.0	0.001045421584213641	0.0	5.018280938769283	1.715563625376231E-4
56-57	1.0722272658601445E-5	0.0010722272658601445	0.0	5.664201365996092	1.7691749886692383E-4
58-59	2.144454531720289E-5	0.0010829495385187458	0.0	6.335764108285947	1.8227863519622455E-4
60-61	2.680568164650361E-5	0.001099032947506648	0.0	7.003836750825427	1.876397715255253E-4
62-63	3.2166817975804335E-5	0.0011472831744703546	0.0	7.620715902556414	1.93000907854826E-4
64-65	3.2166817975804335E-5	0.0012330613557391661	0.0	7.788069134211867	1.93000907854826E-4
66-67	3.2166817975804335E-5	0.0012598670373856697	0.0	7.7963842566586115	1.93000907854826E-4
68-69	3.2166817975804335E-5	0.0012813115827028728	0.0	7.802887315026053	1.9836204418412673E-4
70-71	3.2166817975804335E-5	0.0013027561280200754	0.0	7.80881673180626	2.198065895013296E-4
72-73	3.2166817975804335E-5	0.0013349229459958798	0.0	7.81418322927189	2.680568164650361E-4
74-75	3.2166817975804335E-5	0.0013831731729595865	0.0	7.819094030149529	2.680568164650361E-4
76-77	3.2166817975804335E-5	0.00140997885460609	0.0	7.82238576785572	2.680568164650361E-4
78-79	3.2166817975804335E-5	0.0014207011272646913	0.0	7.824396193979208	2.680568164650361E-4
80-81	3.2166817975804335E-5	0.0014260622635939922	0.0	7.8253611985184826	2.7877908912363756E-4
82-83	3.2166817975804335E-5	0.0014314233999232929	0.0	7.826058146241291	2.89501361782239E-4
84-85	3.2166817975804335E-5	0.0014367845362525935	0.0	7.826465592602318	2.89501361782239E-4
86-87	3.2166817975804335E-5	0.0014582290815697964	0.0	7.8266478712375145	2.948624981115397E-4
88	3.2166817975804335E-5	0.001565451808155811	0.0	7.8266478712375145	3.0022363444084043E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8380	0.0	39.74044	1
GTATCAA	14240	0.0	32.79071	1
TCAACGC	14995	0.0	29.31328	4
ATCAACG	15160	0.0	29.025627	3
CAACGCA	15450	0.0	28.50626	5
AACGCAG	16040	0.0	27.678265	6
TATCAAC	17020	0.0	26.477701	2
TCGGTGG	5955	0.0	25.887205	94
ACGCAGA	19195	0.0	22.991976	7
CGCAGAG	19725	0.0	22.396042	8
GTACATG	31860	0.0	20.67047	1
TACATGG	31625	0.0	20.23948	2
ACATGGG	32365	0.0	19.379818	3
CTCGGTG	8250	0.0	18.363688	94
CATGGGG	22995	0.0	17.793552	4
GCAGAGT	24665	0.0	17.64253	9
TCTCGGT	8535	0.0	16.599463	92-93
AGAGTAC	21360	0.0	16.515947	10-11
CAGAGTA	23860	0.0	15.865277	10-11
GTGGTAT	5540	0.0	15.425771	1
>>END_MODULE