FastQCFastQC Report
Fri 27 May 2016
SRR2140328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8370873
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72100.037.0240551
GTATCAA118250.031.2706491
ATCAACG125400.027.9793193
TCAACGC127150.027.5265754
CAACGCA128100.027.256265
AACGCAG134350.026.145416
TATCAAC142000.025.065412
ACGCAGA155850.022.3516757
CGCAGAG158250.022.0126918
GTACATG248650.019.5808541
TACATGG248900.018.8531632
ACATGGG253250.018.2625143
GCAGAGT199600.017.2847489
GTACTAG22300.016.9394681
ACGGGCG7600.016.5653175
CATGGGG179850.016.403914
CAGAGTA196500.015.31428310-11
AGAGTAC172950.015.17912810-11
GGCGTCG8000.014.1648698
TATACTG45750.013.5757165
Produced by FastQC (version 0.11.5)