##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140328_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8370873
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.769698453195982	33.0	31.0	34.0	30.0	34.0
2	31.80740360055636	33.0	31.0	34.0	30.0	34.0
3	32.02277731366848	34.0	31.0	34.0	30.0	34.0
4	35.48059324278364	37.0	35.0	37.0	33.0	37.0
5	35.56642479225285	37.0	35.0	37.0	33.0	37.0
6	35.61470673369433	37.0	35.0	37.0	33.0	37.0
7	35.50799313285484	37.0	35.0	37.0	33.0	37.0
8	35.4735121414457	37.0	35.0	37.0	33.0	37.0
9	37.12996135528517	39.0	37.0	39.0	34.0	39.0
10-11	37.13469951103069	39.0	37.0	39.0	34.0	39.0
12-13	37.06640974005937	39.0	37.0	39.0	33.5	39.0
14-15	38.40443816314021	40.0	38.0	41.0	33.0	41.0
16-17	38.411163984927256	40.0	38.0	41.0	33.0	41.0
18-19	38.424332922026174	40.0	38.0	41.0	33.5	41.0
20-21	38.476516640621774	40.0	38.0	41.0	34.0	41.0
22-23	38.4379831914101	40.0	38.0	41.0	33.0	41.0
24-25	38.40575523918703	40.0	38.0	41.0	33.0	41.0
26-27	38.360110004075935	40.0	38.0	41.0	33.0	41.0
28-29	38.28479208167826	40.0	38.0	41.0	33.0	41.0
30-31	38.16484541099992	40.0	38.0	41.0	33.0	41.0
32-33	38.114509003401665	40.0	38.0	41.0	33.0	41.0
34-35	37.96466882806783	40.0	38.0	41.0	32.0	41.0
36-37	37.840502095622774	40.0	37.0	41.0	32.0	41.0
38-39	37.824761642019695	40.0	37.0	41.0	32.0	41.0
40-41	37.740944098292665	40.0	37.0	41.0	32.0	41.0
42-43	37.56120238121731	40.0	37.0	41.0	31.0	41.0
44-45	37.61485154508047	40.0	37.0	41.0	31.5	41.0
46-47	37.82409717306568	40.0	37.0	41.0	32.0	41.0
48-49	37.8172157633726	40.0	37.0	41.0	32.0	41.0
50-51	37.72337199766897	40.0	37.0	41.0	32.0	41.0
52-53	37.64594542563557	40.0	37.0	41.0	32.0	41.0
54-55	37.567088008742445	40.0	36.0	41.0	32.0	41.0
56-57	37.6221255685438	40.0	36.0	41.0	32.0	41.0
58-59	37.43174511625759	39.5	36.0	41.0	31.0	41.0
60-61	37.35118526628345	39.0	35.5	41.0	31.0	41.0
62-63	37.125743668826786	39.0	35.0	41.0	31.0	41.0
64-65	36.92291105156424	39.0	35.0	41.0	31.0	41.0
66-67	36.66131997411399	38.0	35.0	40.0	31.0	41.0
68-69	36.43980324334761	37.5	35.0	40.0	31.0	41.0
70-71	36.16764310682663	37.0	35.0	40.0	30.5	41.0
72-73	35.85201637656391	36.5	34.0	39.0	30.0	41.0
74-75	35.52750732606435	36.0	34.0	39.0	30.0	41.0
76-77	35.21123344858308	36.0	34.0	39.0	30.0	40.0
78-79	34.869947078201456	35.0	34.0	37.5	30.0	39.5
80-81	34.56612757329478	35.0	34.0	37.0	30.0	39.0
82-83	34.27178302333061	35.0	34.0	36.5	29.0	39.0
84-85	34.05337382876442	35.0	34.0	36.0	29.0	37.5
86-87	33.83547273213296	35.0	34.0	36.0	29.0	37.0
88-89	33.605071805394836	35.0	33.0	35.5	29.0	37.0
90-91	33.4401639377398	35.0	33.0	35.0	29.0	36.0
92-93	33.353995179373186	35.0	33.0	35.0	29.0	36.0
94-95	33.34224822700813	35.0	33.0	35.0	29.0	36.0
96-97	33.311262935751856	35.0	33.0	35.0	29.0	36.0
98-99	33.360260067548126	35.0	33.0	35.0	29.0	35.5
100	32.73964273867297	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	3.0
15	4.0
16	42.0
17	289.0
18	1795.0
19	5680.0
20	12579.0
21	21687.0
22	32208.0
23	43625.0
24	56470.0
25	70513.0
26	82015.0
27	88327.0
28	98521.0
29	116896.0
30	136671.0
31	164422.0
32	210169.0
33	278211.0
34	388904.0
35	567000.0
36	897073.0
37	1521876.0
38	2118937.0
39	1378045.0
40	78908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.4178393340814	21.08906681537278	14.824272211512469	23.66882163903335
2	14.103963333542834	21.312151200623823	41.84341478487564	22.74047068095771
3	17.534108081087098	26.626537778869526	30.66984123039514	25.169512909648233
4	12.144324805359997	18.707398512362474	39.74868504727283	29.3995916350047
5	13.155401525764693	38.49077991679428	35.64139870285868	12.712419854582349
6	29.048160657386816	38.62784360062174	19.445658420259708	12.878337321731733
7	26.065203507396195	33.222553458785896	23.53101681182856	17.181226221989345
8	23.950558049274928	37.055525601187746	21.63174351106953	17.362172838467796
9	24.927832477445968	18.00710952988816	21.745795180787663	35.319262811878204
10-11	23.502402225319845	27.575749900962563	29.332505592210424	19.589342281507165
12-13	24.167670184989262	25.146782288862862	29.162100083337965	21.52344744280991
14-15	21.687869122025464	26.660347614379553	26.5848499128512	25.06693335074378
16-17	21.495180226387227	29.558018722620965	27.34141326943167	21.605387781560143
18-19	21.489999930111004	29.235050609488788	28.44090852587801	20.834040934522193
20-21	22.494050940908554	28.259179762924624	28.12768572830093	21.119083567865896
22-23	22.255783448847367	28.134477980545018	27.91053306833724	21.699205502270377
24-25	21.896693437705437	28.355435821809056	27.9857160982923	21.762154642193206
26-27	21.926016591557435	28.46840055494241	27.86366176978331	21.741921083716843
28-29	21.82397015820195	28.51936097237039	27.9450881521657	21.711580717261967
30-31	22.102554960742175	28.29652698683188	27.897658620973253	21.703259431452693
32-33	21.92166139682861	28.391906991456604	27.877750055620982	21.808681556093802
34-35	22.11956229107749	28.444084460371442	27.876479647295827	21.55987360125524
36-37	22.079081609362607	28.280415664157577	27.850277638970127	21.790225087509686
38-39	21.850060615779874	28.346463638464712	28.052502680405222	21.750973065350188
40-41	22.068571769727317	28.182597204625743	28.042444867596007	21.706386158050933
42-43	21.97884217039583	28.258608059881894	27.89694621504196	21.86560355468031
44-45	22.080445465011806	28.32908003061702	27.792280873555097	21.79819363081608
46-47	21.997577479840576	28.24431793042983	27.760533421493825	21.997571168235762
48-49	21.9335323583252	28.236438889258476	27.916250351328237	21.91377840108809
50-51	21.87191149064175	28.29643474294356	27.911436962047166	21.920216804367524
52-53	21.905253492790134	28.267217780504655	27.800698768456368	22.02682995824884
54-55	22.012045173909204	28.194775904531134	27.84421277220588	21.948966149353783
56-57	21.984851636221386	28.2431005346427	27.83109579662448	21.940952032511426
58-59	21.960877613460887	28.293814600533057	27.924754332811503	21.820553453194552
60-61	21.962577968172305	28.220489147691495	27.906342667090033	21.91059021704617
62-63	22.031658056790807	28.274912944487866	27.76951610735875	21.923912891362573
64-65	21.94920972282585	28.23804396408807	27.856525167884804	21.956221145201273
66-67	21.97660599549096	28.213022191562075	27.790354491398052	22.020017321548913
68-69	21.901586746474138	28.20476987603116	27.846133845136546	22.047509532358156
70-71	21.871108070158332	28.061809727181565	27.88451345668844	22.18256874597167
72-73	21.970200472479586	28.0996254909722	27.902423472814515	22.0277505637337
74-75	21.83229023061243	28.226479129073507	27.90620802738249	22.035022612931577
76-77	21.899374458040167	28.149761768411913	27.830639997152034	22.12022377639589
78-79	21.88177969462695	28.14100059823288	27.848340923057762	22.12887878408241
80-81	21.894032464058423	28.178922500211662	27.86580910057637	22.061235935153544
82-83	21.927299166261175	28.16578522655603	27.997933856344947	21.90898175083785
84-85	22.063764078436588	28.12458217014505	27.876969029806485	21.934684721611873
86-87	21.84490806321256	28.169147972997727	28.01903466158384	21.966909302205874
88-89	21.963175990305178	28.121758428898108	27.93907603729085	21.97598954350586
90-91	21.95678334261434	28.188553774652835	27.95898677977977	21.89567610295305
92-93	21.919930912119643	28.21751222237457	27.988376209703	21.87418065580279
94-95	21.69948584892607	28.324819717048538	28.058708777643414	21.91698565638198
96-97	21.776978531076345	28.47082072574394	28.070114230425048	21.68208651275467
98-99	22.848790235343092	30.384678079953687	28.245003827141517	18.5215278575617
100	21.919205393390005	31.330880289989544	21.975219304199804	24.774695012420647
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2921.0
1	2291.0
2	1807.0
3	1943.5
4	1921.0
5	2092.5
6	2558.5
7	3026.5
8	3363.5
9	3802.5
10	4275.5
11	4592.0
12	4933.5
13	5459.5
14	6309.5
15	7597.5
16	9162.5
17	11102.5
18	13437.0
19	16318.0
20	20006.0
21	24692.5
22	30710.5
23	39029.0
24	50480.5
25	65012.5
26	83044.0
27	103141.0
28	123728.0
29	147307.0
30	169785.0
31	190001.0
32	210988.5
33	230792.5
34	251051.0
35	270370.5
36	288519.5
37	306577.0
38	319111.5
39	325811.0
40	334278.5
41	341320.5
42	344627.5
43	349421.0
44	353838.5
45	359058.5
46	361930.5
47	360621.0
48	355415.5
49	345124.0
50	334976.5
51	323796.5
52	309891.0
53	292801.0
54	272557.5
55	250002.5
56	228294.5
57	206656.0
58	182958.0
59	158933.5
60	132864.5
61	107808.5
62	86673.5
63	68436.5
64	52704.0
65	40318.5
66	31337.5
67	24965.5
68	19840.5
69	15393.0
70	12042.5
71	9472.5
72	7509.0
73	5909.0
74	4732.0
75	3808.0
76	2913.0
77	2211.5
78	1668.5
79	1203.0
80	876.5
81	655.0
82	508.0
83	370.5
84	285.5
85	231.5
86	191.0
87	157.0
88	99.0
89	56.5
90	42.5
91	36.0
92	30.5
93	20.5
94	14.5
95	11.5
96	11.5
97	11.5
98	7.5
99	13.0
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.021586756841251803
3	0.0120537009700183
4	0.005017397826965001
5	0.009258293609280656
6	0.009114939385653085
7	0.0026162145812031793
8	0.008768500011886454
9	0.004993505456360406
10-11	0.0035958017759915844
12-13	0.008822257845746794
14-15	0.005011424734313852
16-17	0.0036256672392473285
18-19	0.005453433590498864
20-21	0.004070172401983854
22-23	0.00987595595933786
24-25	0.00928614065958541
26-27	0.022495034369511478
28-29	0.019286198177121128
30-31	0.015549940332743458
32-33	0.01215156488805929
34-35	0.008539235394440304
36-37	0.010571257487289386
38-39	0.005194230851097389
40-41	0.00618571108140846
42-43	0.004030101442434783
44-45	0.007089899677606681
46-47	0.009674754079489749
48-49	0.007072218619273762
50-51	0.010022203578883313
52-53	8.093377635835041E-4
54-55	0.002560458165248835
56-57	6.528514937604872E-4
58-59	0.0028706083605036523
60-61	9.552523473981011E-4
62-63	0.007112305477256416
64-65	0.003313507627035972
66-67	0.003729546849659402
68-69	0.005066847344509458
70-71	5.952119450534344E-4
72-73	5.516410533277854E-4
74-75	0.0
76-77	0.0
78-79	3.7806395819176014E-4
80-81	1.4260723707710106E-4
82-83	1.5201172314408889E-5
84-85	8.621124294674646E-4
86-87	0.0031321444723117647
88-89	0.021116084205422853
90-91	0.0027548247089733096
92-93	0.0011705510954557407
94-95	0.0012169305377716067
96-97	4.7031057600218913E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3408.0
20-21	6881.0
22-23	7424.0
24-25	8766.0
26-27	11152.0
28-29	15496.0
30-31	24101.0
32-33	28908.0
34-35	37415.0
36-37	50253.0
38-39	56178.0
40-41	55004.0
42-43	59282.0
44-45	66737.0
46-47	68223.0
48-49	67496.0
50-51	65402.0
52-53	66177.0
54-55	71815.0
56-57	75150.0
58-59	74530.0
60-61	72617.0
62-63	71810.0
64-65	74294.0
66-67	78258.0
68-69	78260.0
70-71	77971.0
72-73	75331.0
74-75	76757.0
76-77	80119.0
78-79	83169.0
80-81	83661.0
82-83	82082.0
84-85	86322.0
86-87	103901.0
88-89	94636.0
90-91	98175.0
92-93	105429.0
94-95	131097.0
96-97	297039.0
98-99	517481.0
100-101	5082666.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.57618030634818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.87303740644947	76.90991253773494
2	6.945913712833544	11.888095285635595
3	1.8039390898887095	4.631226504539574
4	0.6808779400631869	2.3306773346184886
5	0.29303012427962843	1.2538199375272547
6	0.14549710320912945	0.7470651802965486
7	0.08605671951366607	0.5155083741972024
8	0.05183988943137731	0.3549007780032558
9	0.029978930369328614	0.23089341156094104
>10	0.08913465120018385	1.0584566698828468
>50	5.396205149951184E-4	0.031586275515236295
>100	1.1240487584312728E-4	0.019097438566932173
>500	4.24073709318958E-5	0.028760271921188213
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.3892370604595245E-5	0.0
3	0.0	0.0	0.0	2.3892370604595245E-5	0.0
4	0.0	5.973092651148811E-5	0.0	2.3892370604595245E-5	0.0
5	0.0	5.973092651148811E-5	0.0	3.5838555906892864E-5	0.0
6	7.167711181378573E-5	5.973092651148811E-5	0.0	3.5838555906892864E-5	0.0
7	7.167711181378573E-5	5.973092651148811E-5	0.0	3.5838555906892864E-5	0.0
8	7.167711181378573E-5	5.973092651148811E-5	0.0	3.5838555906892864E-5	1.1946185302297622E-5
9	7.167711181378573E-5	7.167711181378573E-5	0.0	5.973092651148811E-5	3.5838555906892864E-5
10-11	7.167711181378573E-5	7.167711181378573E-5	0.0	8.362329711608335E-5	3.5838555906892864E-5
12-13	7.167711181378573E-5	7.167711181378573E-5	0.0	1.015425750695298E-4	4.778474120919049E-5
14-15	7.167711181378573E-5	1.075156677206786E-4	0.0	1.1946185302297622E-4	7.167711181378573E-5
16-17	1.3140803832527385E-4	1.075156677206786E-4	0.0	1.3140803832527385E-4	8.362329711608335E-5
18-19	1.3140803832527385E-4	1.134887603718274E-4	0.0	1.7321968688331554E-4	1.075156677206786E-4
20-21	1.3140803832527385E-4	1.1946185302297622E-4	0.0	2.3295061339480364E-4	1.075156677206786E-4
22-23	1.3738113097642264E-4	1.1946185302297622E-4	0.0	2.687891693016965E-4	1.2543494567412503E-4
24-25	1.4335422362757145E-4	1.3140803832527385E-4	0.0	3.106008178597382E-4	1.4335422362757145E-4
26-27	1.4932731627872027E-4	1.4335422362757145E-4	0.0	4.6590122678960723E-4	1.4335422362757145E-4
28-29	1.553004089298691E-4	1.4335422362757145E-4	0.0	0.0016605197570193696	1.4335422362757145E-4
30-31	1.553004089298691E-4	1.9113896483676196E-4	0.0	0.005543029980266097	1.4335422362757145E-4
32-33	1.672465942321667E-4	1.9113896483676196E-4	0.0	0.01461615771736114	1.4335422362757145E-4
34-35	1.9113896483676196E-4	2.5086989134825006E-4	0.0	0.024740549761058377	1.7919277953446432E-4
36-37	1.9113896483676196E-4	3.106008178597382E-4	0.0	0.037755918647911633	1.8516587218561314E-4
38-39	1.9113896483676196E-4	3.225470031620358E-4	0.0	0.0522287221416452	1.9113896483676196E-4
40-41	1.9113896483676196E-4	3.46439373766631E-4	0.0	0.06630730152040296	1.9113896483676196E-4
42-43	1.9113896483676196E-4	3.643586517200775E-4	0.0	0.08051131584483483	1.9113896483676196E-4
44-45	1.9113896483676196E-4	4.0019720762697034E-4	0.0	0.09475714181782474	1.9711205748791075E-4
46-47	1.9113896483676196E-4	4.1811648558041676E-4	0.0	0.10963014251918528	2.0905824279020838E-4
48-49	1.9113896483676196E-4	4.1811648558041676E-4	0.0	0.12264551140603855	2.2697752074365483E-4
50-51	2.0905824279020838E-4	4.957666900453513E-4	0.0	0.13519497906610217	2.2697752074365483E-4
52-53	2.150313354413572E-4	5.07712875347649E-4	0.0	0.14653788201063378	2.5086989134825006E-4
54-55	2.150313354413572E-4	5.256321533010954E-4	0.0	0.15878272194548884	2.807353546039941E-4
56-57	2.150313354413572E-4	5.256321533010954E-4	0.0	0.17038844096667097	2.867084472551429E-4
58-59	2.21004428092506E-4	5.256321533010954E-4	0.0	0.18237643791752664	2.867084472551429E-4
60-61	2.3892370604595243E-4	5.256321533010954E-4	0.0	0.19339082076624506	2.9865463255744054E-4
62-63	3.46439373766631E-4	5.256321533010954E-4	0.0	0.20320461199208253	3.225470031620358E-4
64-65	3.46439373766631E-4	6.092554504171787E-4	0.0	0.21073668182518118	3.344931884643334E-4
66-67	3.5838555906892865E-4	6.271747283706252E-4	0.0	0.21755795363279315	3.46439373766631E-4
68-69	3.5838555906892865E-4	6.510670989752203E-4	0.0	0.22409251699314992	3.46439373766631E-4
70-71	3.643586517200775E-4	6.689863769286668E-4	0.0	0.22985655140150854	3.46439373766631E-4
72-73	4.1811648558041676E-4	6.749594695798156E-4	0.0	0.23523233478754246	3.942241149758215E-4
74-75	4.1811648558041676E-4	6.928787475332621E-4	0.0	0.24050657559850686	3.942241149758215E-4
76-77	4.539550414873096E-4	7.227442107890061E-4	0.0	0.24418600067161456	3.942241149758215E-4
78-79	4.897935973942024E-4	7.406634887424526E-4	0.0	0.24643188350844647	3.942241149758215E-4
80-81	4.897935973942024E-4	7.406634887424526E-4	0.0	0.24745328235179292	4.121433929292679E-4
82-83	4.957666900453513E-4	7.406634887424526E-4	0.0	0.24845676191718594	4.42008856185012E-4
84-85	5.07712875347649E-4	7.526096740447501E-4	0.0	0.24880320129095257	4.42008856185012E-4
86-87	5.256321533010954E-4	7.526096740447501E-4	0.0	0.24896447479253359	4.5395504148730965E-4
88	5.256321533010954E-4	8.003944152539407E-4	0.0	0.24899434025578934	4.5395504148730965E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7210	0.0	37.024055	1
GTATCAA	11825	0.0	31.270649	1
ATCAACG	12540	0.0	27.979319	3
TCAACGC	12715	0.0	27.526575	4
CAACGCA	12810	0.0	27.25626	5
AACGCAG	13435	0.0	26.14541	6
TATCAAC	14200	0.0	25.06541	2
ACGCAGA	15585	0.0	22.351675	7
CGCAGAG	15825	0.0	22.012691	8
GTACATG	24865	0.0	19.580854	1
TACATGG	24890	0.0	18.853163	2
ACATGGG	25325	0.0	18.262514	3
GCAGAGT	19960	0.0	17.284748	9
GTACTAG	2230	0.0	16.939468	1
ACGGGCG	760	0.0	16.565317	5
CATGGGG	17985	0.0	16.40391	4
CAGAGTA	19650	0.0	15.314283	10-11
AGAGTAC	17295	0.0	15.179128	10-11
GGCGTCG	800	0.0	14.164869	8
TATACTG	4575	0.0	13.575716	5
>>END_MODULE