FastQCFastQC Report
Fri 27 May 2016
SRR2140327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7605668
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63050.038.5976071
GTATCAA102600.029.8135831
ATCAACG109200.026.3851173
TCAACGC110000.026.1918454
CAACGCA114300.025.3170455
AACGCAG117000.024.6249756
TATCAAC122950.023.986352
ACGCAGA137800.020.817537
CGCAGAG141900.020.1861328
GTACATG218250.018.719541
TACATGG217850.018.4442162
ACATGGG222100.017.6330913
GCAGAGT181050.015.5870469
CATGGGG160200.015.4001434
AGAGTAC155550.014.81501210-11
GTATAAT47100.014.0832111
CAGAGTA178800.014.06991710-11
GTGTAGC42200.013.6159961
GTATAAG34300.013.426271
ATGGGGG97600.013.1580785
Produced by FastQC (version 0.11.5)