##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140327_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7605668
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.059761614627405	33.0	31.0	34.0	30.0	34.0
2	32.09844473884477	34.0	31.0	34.0	30.0	34.0
3	32.29493043872017	34.0	31.0	34.0	30.0	34.0
4	35.70590315012436	37.0	35.0	37.0	35.0	37.0
5	35.78162970037609	37.0	35.0	37.0	35.0	37.0
6	35.83441362415504	37.0	36.0	37.0	35.0	37.0
7	35.74483858617021	37.0	36.0	37.0	35.0	37.0
8	35.70360920303121	37.0	36.0	37.0	35.0	37.0
9	37.41396035167457	39.0	38.0	39.0	35.0	39.0
10-11	37.416768191827465	39.0	38.0	39.0	35.0	39.0
12-13	37.353177656453056	39.0	38.0	39.0	34.5	39.0
14-15	38.764977974321255	41.0	39.0	41.0	35.0	41.0
16-17	38.77492266294033	41.0	39.0	41.0	34.5	41.0
18-19	38.79507342418838	41.0	38.5	41.0	34.0	41.0
20-21	38.84167831118192	41.0	39.0	41.0	34.0	41.0
22-23	38.80542134832555	40.0	39.0	41.0	34.0	41.0
24-25	38.776009358306105	40.0	38.5	41.0	34.0	41.0
26-27	38.732136822545925	40.0	38.0	41.0	34.0	41.0
28-29	38.662888696242995	40.0	38.0	41.0	34.0	41.0
30-31	38.542585704758025	40.0	38.0	41.0	34.0	41.0
32-33	38.4896107972036	40.0	38.0	41.0	34.0	41.0
34-35	38.34092637014781	40.0	38.0	41.0	33.0	41.0
36-37	38.22634949446392	40.0	38.0	41.0	33.0	41.0
38-39	38.21301800347817	40.0	38.0	41.0	33.0	41.0
40-41	38.1290988647182	40.0	38.0	41.0	33.0	41.0
42-43	37.95162668050866	40.0	38.0	41.0	33.0	41.0
44-45	38.013716327834835	40.0	37.5	41.0	32.5	41.0
46-47	38.224741471292	40.0	38.0	41.0	33.0	41.0
48-49	38.221720862080964	40.0	38.0	41.0	33.0	41.0
50-51	38.12748876756247	40.0	38.0	41.0	33.0	41.0
52-53	38.05390708079077	40.0	37.0	41.0	33.0	41.0
54-55	37.976596451556006	40.0	37.0	41.0	33.0	41.0
56-57	38.028166503485814	40.0	37.0	41.0	33.0	41.0
58-59	37.83689418209658	40.0	36.5	41.0	32.5	41.0
60-61	37.754448139434025	40.0	36.0	41.0	32.0	41.0
62-63	37.53928457882253	39.0	36.0	41.0	32.0	41.0
64-65	37.327066141097546	39.0	35.0	41.0	32.0	41.0
66-67	37.054333581012344	39.0	35.0	41.0	31.5	41.0
68-69	36.819234055103564	38.0	35.0	40.5	31.0	41.0
70-71	36.53251966195372	37.0	35.0	40.0	31.0	41.0
72-73	36.20795614124508	37.0	35.0	39.5	31.0	41.0
74-75	35.87543763772356	36.0	35.0	39.0	31.0	41.0
76-77	35.54848800040007	36.0	34.5	39.0	31.0	40.5
78-79	35.20449965722837	35.5	34.0	37.5	30.5	39.5
80-81	34.890186841511024	35.0	34.0	37.0	30.0	39.0
82-83	34.58477097882346	35.0	34.0	37.0	30.0	39.0
84-85	34.36026134696401	35.0	34.0	36.0	30.0	38.0
86-87	34.14117671326836	35.0	34.0	36.0	30.0	37.0
88-89	33.910292657492136	35.0	34.0	36.0	30.0	37.0
90-91	33.7393977720972	35.0	34.0	35.0	30.0	36.5
92-93	33.64023801908769	35.0	34.0	35.0	30.0	36.0
94-95	33.612980006831506	35.0	34.0	35.0	30.0	36.0
96-97	33.567367222109496	35.0	34.0	35.0	30.0	36.0
98-99	33.59786945649074	35.0	34.0	35.0	30.0	36.0
100	33.00762029688091	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	4.0
15	8.0
16	33.0
17	234.0
18	1221.0
19	3787.0
20	8147.0
21	14690.0
22	22377.0
23	30889.0
24	40620.0
25	51252.0
26	60096.0
27	65203.0
28	74284.0
29	90401.0
30	104821.0
31	125738.0
32	161276.0
33	216649.0
34	306113.0
35	455530.0
36	745245.0
37	1363833.0
38	2091278.0
39	1473843.0
40	98094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.6522609190935	20.91232486088007	14.698235579044471	23.737178640981963
2	14.055041581224067	21.33545448842739	42.09309717619584	22.516406754152698
3	17.604691835312373	26.56708351414257	30.678356805659657	25.1498678448854
4	12.096398817663518	18.702887993604445	39.96603675341342	29.23467643531862
5	13.187073716267125	38.42207135596853	35.694710169796856	12.696144757967486
6	28.95915773462087	38.620638962663314	19.642383301332515	12.777820001383297
7	26.027124023806657	33.16424372833375	23.63883196687632	17.16980028098327
8	23.81247112095026	37.17292873603845	21.729208464858747	17.28539167815254
9	24.856733854017442	18.08315070732051	21.85879203723848	35.201323401423565
10-11	23.48903935424036	27.585126217092572	29.43474731040317	19.491087118263895
12-13	24.17116869354578	25.039649898773842	29.34286460599181	21.44631680168857
14-15	21.617596687570963	26.63792791732602	26.743610865850698	25.000864529252315
16-17	21.447507224801747	29.581393428536757	27.480320230404743	21.490779116256753
18-19	21.47906706857306	29.258492172548884	28.469057606035093	20.793383152842964
20-21	22.477722829587407	28.31028570135594	28.210923785608742	21.001067683447907
22-23	22.171447160354035	28.208924777451266	27.97497024883291	21.64465781336179
24-25	21.903503040401162	28.331265030983033	28.063161928937923	21.70206999967788
26-27	21.897321578705885	28.518900587920403	27.901220826037466	21.682557007336246
28-29	21.814130105902148	28.603337962001085	27.949527913520562	21.6330040185762
30-31	22.09953488532061	28.346359304628354	27.9222386354061	21.631867174644935
32-33	21.86767213999225	28.473753375610904	27.908754873966696	21.749819610430148
34-35	22.05282969959182	28.481980306345005	27.905031012479974	21.5601589815832
36-37	22.030575201925863	28.29809127703386	27.93194244405265	21.739391076987626
38-39	21.833301500140074	28.362877696468114	28.09019463022771	21.713626173164098
40-41	22.07626633893795	28.262779583245923	28.002295598413973	21.65865847940216
42-43	21.957092241360296	28.291947640149523	27.93770524124075	21.813254877249435
44-45	22.033088464705937	28.383428201926446	27.851121794542124	21.732361538825494
46-47	21.99181883462388	28.26282911336978	27.79832288943704	21.947029162569297
48-49	21.908272678710766	28.266231740028484	27.97445265901308	21.851042922247665
50-51	21.855611671852575	28.323141354900876	27.956495679157527	21.864751294089018
52-53	21.906151501531227	28.26267570590363	27.891226111488265	21.93994668107688
54-55	21.975750272917054	28.194989320699083	27.919884075130135	21.90937633125373
56-57	21.989873132133432	28.284023123046175	27.877585500329527	21.848518244490865
58-59	21.945627206627247	28.37458212658382	27.938199784124944	21.74159088266399
60-61	21.962226310326248	28.28421735728055	27.942363116623035	21.811193215770164
62-63	21.99218200328367	28.301287421158506	27.830967183842816	21.875563391715012
64-65	21.94734609702288	28.31070429511065	27.85493232156049	21.887017286305984
66-67	21.982680111182955	28.31007301471392	27.799040275302694	21.90820659880043
68-69	21.955782584257953	28.23499424917562	27.913181674217764	21.896041492348658
70-71	21.900282755697813	28.103728619946377	27.91929791052071	22.076690713835095
72-73	21.973117905320315	28.125681342201165	27.942831214061087	21.95836953841743
74-75	21.861360027308102	28.228278157660814	27.91995934659032	21.99040246844076
76-77	21.9301346367089	28.258080654643052	27.79478331317509	22.01700139547296
78-79	21.907253682753396	28.201204685014996	27.83870251906838	22.052839113163223
80-81	21.900903028486645	28.22655032951485	27.890660977489173	21.981885664509328
82-83	21.907656159983237	28.215177760297	28.01475606196504	21.862410017754723
84-85	22.023382805773196	28.2255253899039	27.906188161883172	21.844903642439736
86-87	21.851206203515996	28.295499480931813	27.956253156438592	21.897041159113595
88-89	21.961827239266373	28.215557344051263	27.903985526056434	21.918629890625926
90-91	21.915433327277146	28.278634461663977	27.950390763406972	21.855541447651905
92-93	21.91528358086636	28.343833057563973	27.93877178307148	21.802111578498184
94-95	21.76214266355467	28.376228324743742	28.014875677973855	21.846753333727733
96-97	21.796273607464254	28.506735642349835	28.07169515718214	21.62529559300377
98-99	22.8769087473593	30.39891047350215	28.3024972912228	18.42168348791575
100	21.85430170852596	31.44014434026044	22.061731897900987	24.64382205331261
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2354.0
1	1917.0
2	1583.0
3	1648.5
4	1636.0
5	1811.5
6	2164.0
7	2537.0
8	2849.0
9	3153.5
10	3459.0
11	3720.0
12	4080.0
13	4620.0
14	5417.0
15	6583.5
16	8056.0
17	9826.0
18	11956.5
19	14654.5
20	18034.5
21	22373.5
22	28237.0
23	36132.0
24	46928.5
25	60672.0
26	77310.0
27	95863.5
28	115296.0
29	135957.0
30	155425.5
31	173441.5
32	191776.5
33	209211.5
34	226426.0
35	242429.0
36	258641.0
37	272750.5
38	281712.5
39	290358.0
40	300583.0
41	307944.5
42	311422.0
43	314682.5
44	318149.0
45	322587.0
46	324969.0
47	324016.5
48	319598.0
49	311079.5
50	302450.0
51	292288.5
52	278186.0
53	261389.5
54	243386.0
55	223625.0
56	203116.0
57	182469.0
58	161317.5
59	140080.0
60	117289.0
61	95456.0
62	76502.0
63	60243.0
64	46250.0
65	35398.5
66	27324.5
67	21405.5
68	16999.0
69	13092.5
70	10234.5
71	8147.0
72	6441.5
73	5046.0
74	4038.0
75	3256.5
76	2523.5
77	1953.0
78	1468.5
79	1062.5
80	813.0
81	613.0
82	474.0
83	333.0
84	232.5
85	184.5
86	157.0
87	132.0
88	91.0
89	55.5
90	37.5
91	32.5
92	26.0
93	16.5
94	14.0
95	14.0
96	13.5
97	10.0
98	5.5
99	10.0
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.021497125564776162
3	0.012727350181469924
4	0.005101458543812326
5	0.009177366143250008
6	0.008598850225910466
7	0.003063504744093484
8	0.00886181200651935
9	0.005482753125695205
10-11	0.003937852664618019
12-13	0.008776349427821462
14-15	0.004904237208355663
16-17	0.0035368359491894726
18-19	0.005449882903119095
20-21	0.0044068284003383916
22-23	0.010274196654574136
24-25	0.009418951470466284
26-27	0.022810522744662304
28-29	0.01977570417447557
30-31	0.015183600547508997
32-33	0.012306289488618637
34-35	0.008608754144958779
36-37	0.009996968165470138
38-39	0.005203296631268788
40-41	0.005912514404988971
42-43	0.004139033399548791
44-45	0.0065412878685028346
46-47	0.009814057476234948
48-49	0.0077271271983380746
50-51	0.01036793526362372
52-53	0.0010124998415756367
54-55	0.0029341604381793816
56-57	5.47465354267352E-4
58-59	0.0030613964883673475
60-61	0.001328697719873963
62-63	0.007142731711068796
64-65	0.0038517407400039103
66-67	0.00410954816499987
68-69	0.00486241057401024
70-71	8.176968103653099E-4
72-73	5.144752683553668E-4
74-75	0.0
76-77	1.5922783417185366E-5
78-79	4.7505666661110915E-4
80-81	2.9326690946451334E-4
82-83	0.0
84-85	0.0010176778992725772
86-87	0.0037715164591176917
88-89	0.02192852826888425
90-91	0.0026016571424842348
92-93	0.0015719313119003148
94-95	0.001220892377169923
96-97	6.157192386438627E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2622.0
20-21	4951.0
22-23	5467.0
24-25	6548.0
26-27	8469.0
28-29	11984.0
30-31	19416.0
32-33	23555.0
34-35	30413.0
36-37	42815.0
38-39	46938.0
40-41	46011.0
42-43	50337.0
44-45	56818.0
46-47	58597.0
48-49	58431.0
50-51	56388.0
52-53	57014.0
54-55	61758.0
56-57	64933.0
58-59	64726.0
60-61	63709.0
62-63	62470.0
64-65	65426.0
66-67	68518.0
68-69	68447.0
70-71	68167.0
72-73	66030.0
74-75	67126.0
76-77	69681.0
78-79	72117.0
80-81	72343.0
82-83	71334.0
84-85	73478.0
86-87	90795.0
88-89	80624.0
90-91	83953.0
92-93	88663.0
94-95	110382.0
96-97	261777.0
98-99	471441.0
100-101	4750996.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.39959756840722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.32168806132239	78.03757500197466
2	6.806797568942444	11.762091413724795
3	1.641971919268776	4.255971391303424
4	0.6023375363151532	2.0816688295190042
5	0.25451568897851246	1.0995026551294687
6	0.15115123023221694	0.7835643278419926
7	0.08168907361881168	0.4940532160480924
8	0.04169853030712841	0.2882188990183941
9	0.02664984155357859	0.20722820269419057
>10	0.07060553393719912	0.8758669712113578
>50	5.433970704191951E-4	0.03318732882421011
>100	3.069532265211187E-4	0.04826979220741116
>500	2.2332663686805948E-5	0.012117468214471464
>1k	2.2332563131333152E-5	0.020684502288579452
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6296178060888273E-5	0.0	0.0	0.0
3	0.0	3.944426709133241E-5	0.0	0.0	0.0
4	0.0	6.574044515222069E-5	0.0	0.0	0.0
5	0.0	6.574044515222069E-5	0.0	0.0	0.0
6	0.0	6.574044515222069E-5	0.0	0.0	0.0
7	0.0	6.574044515222069E-5	0.0	0.0	0.0
8	0.0	7.888853418266483E-5	0.0	0.0	0.0
9	0.0	1.5777706836532965E-4	0.0	0.0	0.0
10-11	0.0	1.5777706836532965E-4	0.0	0.0	0.0
12-13	1.3148089030444136E-5	1.5777706836532965E-4	0.0	0.0	0.0
14-15	1.3148089030444136E-5	2.1036942448710618E-4	0.0	0.0	0.0
16-17	1.3148089030444136E-5	2.1036942448710618E-4	0.0	0.0	0.0
18-19	1.3148089030444136E-5	2.1036942448710618E-4	0.0	6.574044515222068E-6	0.0
20-21	1.9722133545666203E-5	2.2351751351755032E-4	0.0	3.9444267091332406E-5	0.0
22-23	2.6296178060888273E-5	2.563877360936607E-4	0.0	7.888853418266483E-5	0.0
24-25	1.6435111288055172E-4	2.6296178060888274E-4	0.0	1.709251573957738E-4	0.0
26-27	3.155541367306593E-4	2.6296178060888274E-4	0.0	4.075907599437683E-4	0.0
28-29	3.2870222576110344E-4	2.6296178060888274E-4	0.0	0.002136564467447172	0.0
30-31	3.2870222576110344E-4	3.0898009221543726E-4	0.0	0.008243851822088474	0.0
32-33	3.2870222576110344E-4	3.2212818124588134E-4	0.0	0.023410172518705787	0.0
34-35	3.2870222576110344E-4	3.747205373676579E-4	0.0	0.03834640165729033	0.0
36-37	3.418503147915476E-4	4.2073884897421236E-4	0.0	0.05843668169580897	0.0
38-39	3.418503147915476E-4	4.338869380046565E-4	0.0	0.0783757587104775	0.0
40-41	3.9444267091332414E-4	4.4703502703510064E-4	0.0	0.095264479070083	0.0
42-43	3.9444267091332414E-4	4.601831160655448E-4	0.0	0.11189681169359483	0.0
44-45	3.9444267091332414E-4	5.193495167025434E-4	0.0	0.12786516582106924	0.0
46-47	3.9444267091332414E-4	5.653678283090978E-4	0.0	0.14495768156064662	0.0
48-49	4.010167154285462E-4	5.653678283090978E-4	0.0	0.15759956916341866	0.0
50-51	4.075907599437683E-4	6.70552540552651E-4	0.0	0.16826266936710885	0.0
52-53	4.075907599437683E-4	6.70552540552651E-4	0.0	0.17779503391418083	0.0
54-55	4.075907599437683E-4	6.70552540552651E-4	0.0	0.18599286742466276	0.0
56-57	4.075907599437683E-4	6.70552540552651E-4	0.0	0.19403949791129457	0.0
58-59	4.075907599437683E-4	6.771265850678731E-4	0.0	0.20203353604180463	0.0
60-61	4.075907599437683E-4	7.099968076439834E-4	0.0	0.20910720794018356	0.0
62-63	4.075907599437683E-4	7.165708521592055E-4	0.0	0.21627949050629083	0.0
64-65	4.075907599437683E-4	7.6258916376576E-4	0.0	0.22283381288796725	0.0
66-67	4.075907599437683E-4	7.6258916376576E-4	0.0	0.22909230326645863	0.0
68-69	4.2073884897421236E-4	7.757372527962042E-4	0.0	0.23565319969265025	0.0
70-71	4.338869380046565E-4	7.888853418266483E-4	0.0	0.24160928402344145	0.0
72-73	4.4703502703510064E-4	7.954593863418703E-4	0.0	0.24754564622068698	0.0
74-75	4.4703502703510064E-4	8.151815198875366E-4	0.0	0.25271284520965154	0.0
76-77	4.5360907155032274E-4	8.349036534332026E-4	0.0	0.2564534765388129	0.0
78-79	4.601831160655448E-4	8.414776979484247E-4	0.0	0.25847170820498605	0.0
80-81	4.601831160655448E-4	8.414776979484247E-4	0.0	0.2594578148822694	0.0
82-83	4.601831160655448E-4	8.480517424636468E-4	0.0	0.26022697809055034	0.0
84-85	4.601831160655448E-4	8.546257869788689E-4	0.0	0.2604504956040679	0.0
86-87	4.601831160655448E-4	8.809219650397572E-4	0.0	0.26053595818276576	0.0
88	4.601831160655448E-4	9.861066772833102E-4	0.0	0.260542532227281	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6305	0.0	38.597607	1
GTATCAA	10260	0.0	29.813583	1
ATCAACG	10920	0.0	26.385117	3
TCAACGC	11000	0.0	26.191845	4
CAACGCA	11430	0.0	25.317045	5
AACGCAG	11700	0.0	24.624975	6
TATCAAC	12295	0.0	23.98635	2
ACGCAGA	13780	0.0	20.81753	7
CGCAGAG	14190	0.0	20.186132	8
GTACATG	21825	0.0	18.71954	1
TACATGG	21785	0.0	18.444216	2
ACATGGG	22210	0.0	17.633091	3
GCAGAGT	18105	0.0	15.587046	9
CATGGGG	16020	0.0	15.400143	4
AGAGTAC	15555	0.0	14.815012	10-11
GTATAAT	4710	0.0	14.083211	1
CAGAGTA	17880	0.0	14.069917	10-11
GTGTAGC	4220	0.0	13.615996	1
GTATAAG	3430	0.0	13.42627	1
ATGGGGG	9760	0.0	13.158078	5
>>END_MODULE