FastQCFastQC Report
Fri 27 May 2016
SRR2140326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7758182
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64650.038.3655431
GTATCAA108000.030.7643571
TCAACGC116850.026.9534444
ATCAACG117700.026.6167263
CAACGCA120150.026.1422185
AACGCAG125000.025.1951056
TATCAAC131550.024.2973842
ACGCAGA150500.020.787767
CGCAGAG152300.020.5145598
GTACATG234700.019.341951
TACATGG229800.019.2785472
ACATGGG237650.018.2674963
CATGGGG175650.016.6121754
GCAGAGT189950.016.2931949
AGAGTAC166400.014.99301610-11
TATACCG5350.014.9529615
CAGAGTA189000.014.57034810-11
GTGTAGC41150.013.4059011
ACCGTGC11000.013.3978028
GTATAAT44200.013.147751
Produced by FastQC (version 0.11.5)