##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140326_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7758182
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.058851029790226	33.0	31.0	34.0	30.0	34.0
2	32.09641524264319	34.0	31.0	34.0	30.0	34.0
3	32.28891304689681	34.0	31.0	34.0	30.0	34.0
4	35.697626712031244	37.0	35.0	37.0	35.0	37.0
5	35.77328722115568	37.0	35.0	37.0	35.0	37.0
6	35.82735014466018	37.0	36.0	37.0	35.0	37.0
7	35.737368368001675	37.0	36.0	37.0	35.0	37.0
8	35.6946523038516	37.0	36.0	37.0	35.0	37.0
9	37.40432230128141	39.0	38.0	39.0	35.0	39.0
10-11	37.40343195093902	39.0	38.0	39.0	35.0	39.0
12-13	37.34426422324199	39.0	38.0	39.0	34.5	39.0
14-15	38.75675602866754	41.0	39.0	41.0	35.0	41.0
16-17	38.76922898947202	41.0	39.0	41.0	34.5	41.0
18-19	38.78872917650037	41.0	38.5	41.0	34.0	41.0
20-21	38.831638802058045	41.0	39.0	41.0	34.0	41.0
22-23	38.79763564106821	40.0	39.0	41.0	34.0	41.0
24-25	38.76445273859032	40.0	38.5	41.0	34.0	41.0
26-27	38.71864266429594	40.0	38.0	41.0	34.0	41.0
28-29	38.64821529231277	40.0	38.0	41.0	34.0	41.0
30-31	38.52622797522466	40.0	38.0	41.0	34.0	41.0
32-33	38.47091948280759	40.0	38.0	41.0	34.0	41.0
34-35	38.321294830658815	40.0	38.0	41.0	33.0	41.0
36-37	38.20391340463581	40.0	38.0	41.0	33.0	41.0
38-39	38.19105283734199	40.0	38.0	41.0	33.0	41.0
40-41	38.10525560146093	40.0	38.0	41.0	33.0	41.0
42-43	37.92508526486155	40.0	37.5	41.0	33.0	41.0
44-45	37.9867814340231	40.0	37.5	41.0	32.5	41.0
46-47	38.196067726543355	40.0	38.0	41.0	33.0	41.0
48-49	38.19145033250558	40.0	38.0	41.0	33.0	41.0
50-51	38.09685026116459	40.0	37.5	41.0	33.0	41.0
52-53	38.019548120023224	40.0	37.0	41.0	33.0	41.0
54-55	37.93671904385322	40.0	37.0	41.0	33.0	41.0
56-57	37.98193504071817	40.0	37.0	41.0	33.0	41.0
58-59	37.783569405993944	40.0	36.5	41.0	32.5	41.0
60-61	37.69368446288737	39.5	36.0	41.0	32.0	41.0
62-63	37.472587468192415	39.0	35.5	41.0	32.0	41.0
64-65	37.25170155005075	39.0	35.0	41.0	32.0	41.0
66-67	36.96985577568225	38.5	35.0	41.0	31.0	41.0
68-69	36.73041940328803	38.0	35.0	40.0	31.0	41.0
70-71	36.44057901627973	37.0	35.0	40.0	31.0	41.0
72-73	36.116032631212406	37.0	35.0	39.0	31.0	41.0
74-75	35.78659655960499	36.0	35.0	39.0	31.0	41.0
76-77	35.46234055454158	36.0	34.0	39.0	31.0	40.5
78-79	35.12474723266949	35.0	34.0	37.5	30.5	39.5
80-81	34.81681116573989	35.0	34.0	37.0	30.0	39.0
82-83	34.51661025991252	35.0	34.0	37.0	30.0	39.0
84-85	34.30381958611001	35.0	34.0	36.0	30.0	37.5
86-87	34.090681182620195	35.0	34.0	36.0	30.0	37.0
88-89	33.862996433615166	35.0	34.0	35.5	30.0	37.0
90-91	33.700708951789665	35.0	34.0	35.0	30.0	36.0
92-93	33.60584383632521	35.0	34.0	35.0	30.0	36.0
94-95	33.58231032975119	35.0	34.0	35.0	30.0	36.0
96-97	33.53620952773248	35.0	34.0	35.0	30.0	36.0
98-99	33.57032870554536	35.0	34.0	35.0	30.0	36.0
100	32.98367133272661	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	4.0
15	6.0
16	45.0
17	260.0
18	1341.0
19	4069.0
20	8599.0
21	15320.0
22	23125.0
23	31836.0
24	41818.0
25	52892.0
26	62053.0
27	66595.0
28	76320.0
29	92908.0
30	107927.0
31	129503.0
32	167044.0
33	223714.0
34	316487.0
35	473224.0
36	775928.0
37	1412704.0
38	2110486.0
39	1466356.0
40	97614.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.73285468167671	20.797088802505538	14.694653979501899	23.775402536315855
2	14.223064526526139	21.266524850125702	41.72100818668214	22.789402436666023
3	17.658613216259738	26.403165999155366	30.602273612084403	25.335947172500497
4	12.261136315942258	18.615074018674434	39.50174096377156	29.622048701611753
5	13.352016376452147	38.29028241129851	35.46139983602923	12.896301376220112
6	29.25902388278653	38.283899260970394	19.46188641399838	12.9951904422447
7	26.201924998034272	33.058895865263025	23.438262526215112	17.30091661048759
8	23.988885601749534	36.94110413077401	21.616756934091566	17.453253333384897
9	24.99830814412369	17.957171361346514	21.703320536242902	35.34119995828689
10-11	23.647116336190965	27.429275965445466	29.22891652458	19.694691173783568
12-13	24.373957616550364	24.949261755971218	29.054960243440448	21.621820384037978
14-15	21.82148875277503	26.462293929240914	26.541639852264865	25.17457746571919
16-17	21.636759515802755	29.389917066814007	27.233616849673265	21.73970656770997
18-19	21.6601190368004	29.045689553272343	28.346171431503052	20.94801997842421
20-21	22.668356662341083	28.099137901016086	28.007706091387185	21.224799345255644
22-23	22.413724787418428	27.993027074069882	27.753299672030096	21.839948466481594
24-25	22.120524826074398	28.143511170162512	27.849957727503433	21.88600627625966
26-27	22.10896596107593	28.297778372876547	27.72224013481013	21.871015531237394
28-29	22.005570651971617	28.382858259617606	27.74868794748851	21.86288314092227
30-31	22.308626373968483	28.16353908961097	27.694746282813355	21.833088253607187
32-33	22.071692434501582	28.25391769142606	27.663915382014466	22.010474492057895
34-35	22.239629633061757	28.252264772526896	27.740329402711396	21.767776191699948
36-37	22.226732620755925	28.093279499628977	27.74929605331606	21.930691826299046
38-39	22.039175561828507	28.178360912138743	27.897726987899485	21.88473653813326
40-41	22.26549600836501	28.027770493435796	27.825104977510744	21.881628520688455
42-43	22.13121709950433	28.07474217504434	27.7569262267149	22.037114498736436
44-45	22.220609534155006	28.165645713944116	27.676660141563197	21.93708461033769
46-47	22.20049737559569	28.083264389150926	27.571579538145023	22.144658697108362
48-49	22.09789194037358	28.108497574821378	27.74789861090681	22.04571187389824
50-51	22.025761857191675	28.169446501148236	27.750364391466427	22.054427250193662
52-53	22.083122990142545	28.099612040757467	27.657078960195474	22.16018600890452
54-55	22.177419213346468	27.998666296396184	27.68719274690939	22.136721743347955
56-57	22.130892889397312	28.103820395037417	27.686002982082712	22.079283733482555
58-59	22.175821634069795	28.18105856136625	27.68927145116498	21.95384835339897
60-61	22.17473554597862	28.037712344076393	27.732344906901712	22.055207203043274
62-63	22.216585451224248	28.079053343323473	27.636687139156418	22.067674066295865
64-65	22.138687445400418	28.10742436496983	27.627297784135944	22.12659040549381
66-67	22.25153796686925	28.08085124491027	27.534717892849248	22.132892895371235
68-69	22.149594158450604	28.066564135583782	27.63997045745152	22.143871248514095
70-71	22.128283500685598	27.89469898943946	27.71052736414026	22.266490145734686
72-73	22.171210967648573	27.94660376861141	27.678351514354137	22.203833749385872
74-75	22.073911746519048	28.079977192431866	27.67695285918036	22.16915820186873
76-77	22.17353749755976	28.03669802608864	27.593845535043375	22.195918941308218
78-79	22.113043792774377	28.004267153372286	27.619754344559123	22.262934709294218
80-81	22.127121944884927	28.030684046955518	27.65984579932414	22.182348208835414
82-83	22.10970984436293	28.044164703308617	27.75964441357406	22.086481038754396
84-85	22.238234250867098	28.044985720271676	27.671918645042677	22.04486138381855
86-87	22.044051034492334	28.07701658866055	27.73412477739172	22.144807599455394
88-89	22.14786842429059	27.994428531773085	27.72517882967749	22.13252421425884
90-91	22.16255563322923	28.10140419928853	27.71215142144974	22.023888746032505
92-93	22.111088768381983	28.06833481428017	27.755538550202175	22.065037867135672
94-95	21.974769348514908	28.17102816848413	27.78660235522966	22.067600127771303
96-97	22.00661264131906	28.33405100269313	27.835187277079836	21.824149078907972
98-99	23.112866204896733	30.20990879804119	28.044793237379466	18.632431759682618
100	22.1024749935479	31.197290014241634	21.798646469944714	24.901588522265747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2798.0
1	2181.0
2	1697.0
3	1813.5
4	1770.5
5	1938.0
6	2371.0
7	2845.5
8	3244.0
9	3628.0
10	3977.0
11	4238.5
12	4584.5
13	5083.0
14	5948.5
15	7160.5
16	8738.5
17	10590.0
18	12617.0
19	15130.5
20	18140.0
21	22311.5
22	28257.5
23	35998.5
24	46144.0
25	59455.0
26	76013.0
27	93927.0
28	112337.5
29	132356.5
30	151203.5
31	168370.0
32	187811.0
33	206685.5
34	223507.5
35	239455.0
36	256188.0
37	272068.5
38	282179.0
39	288401.5
40	297528.0
41	306790.5
42	310996.5
43	316743.5
44	322797.0
45	328345.0
46	331623.0
47	329756.0
48	326495.0
49	320578.5
50	312699.0
51	303405.0
52	291034.5
53	275850.5
54	259859.5
55	241055.5
56	220816.0
57	199993.5
58	177795.0
59	155888.5
60	131427.5
61	106887.0
62	86133.5
63	68233.0
64	52779.5
65	40741.0
66	31337.0
67	24226.0
68	18928.5
69	14774.0
70	11604.0
71	8972.5
72	6964.5
73	5435.5
74	4262.0
75	3430.0
76	2663.5
77	2040.0
78	1525.0
79	1095.5
80	823.0
81	621.0
82	490.5
83	346.5
84	235.0
85	181.5
86	160.0
87	131.5
88	89.5
89	64.0
90	47.0
91	31.5
92	22.5
93	14.5
94	9.5
95	9.0
96	8.0
97	5.5
98	5.0
99	9.5
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02168033696554167
3	0.013018513873482216
4	0.005284743255571989
5	0.009048511622954966
6	0.009177407799920136
7	0.0032481836595222953
8	0.00872627118054204
9	0.005529645991805812
10-11	0.003860440500106855
12-13	0.009280524741492272
14-15	0.004891609915828218
16-17	0.004002226294768542
18-19	0.005323412108661539
20-21	0.0042041775596993215
22-23	0.010885541722823135
24-25	0.009783296432332072
26-27	0.023017781802029236
28-29	0.020024805128680084
30-31	0.015848974576532778
32-33	0.012634806492378735
34-35	0.008698235628482109
36-37	0.010448872141268228
38-39	0.005368865461112331
40-41	0.006233532835150806
42-43	0.004490102131114724
44-45	0.007445037983156712
46-47	0.010018190209866152
48-49	0.00753586826891592
50-51	0.01011089686898997
52-53	8.141128480088887E-4
54-55	0.003024673051089465
56-57	7.720772114044445E-4
58-59	0.002696277563537745
60-61	9.821578745121437E-4
62-63	0.006824912769083671
64-65	0.0034014865085044157
66-67	0.0037568039997985456
68-69	0.005542116778792387
70-71	7.070315811240814E-4
72-73	6.455349767373017E-4
74-75	0.0
76-77	3.1423568572000706E-5
78-79	4.213080773184584E-4
80-81	1.4482390901132176E-4
82-83	0.0
84-85	7.997705235751326E-4
86-87	0.0036129545496171984
88-89	0.02236171077444785
90-91	0.0029460569224714107
92-93	0.0012245103948250091
94-95	0.0011919486536984432
96-97	5.102320662995547E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2761.0
20-21	5189.0
22-23	5806.0
24-25	7050.0
26-27	9112.0
28-29	12960.0
30-31	20574.0
32-33	24677.0
34-35	32150.0
36-37	44671.0
38-39	49486.0
40-41	48268.0
42-43	52865.0
44-45	60268.0
46-47	61944.0
48-49	61008.0
50-51	58800.0
52-53	59861.0
54-55	64982.0
56-57	67663.0
58-59	67757.0
60-61	66673.0
62-63	65741.0
64-65	68754.0
66-67	72044.0
68-69	72248.0
70-71	71415.0
72-73	69425.0
74-75	71204.0
76-77	74113.0
78-79	75136.0
80-81	76152.0
82-83	74172.0
84-85	77884.0
86-87	94981.0
88-89	84213.0
90-91	87349.0
92-93	91981.0
94-95	114726.0
96-97	268604.0
98-99	478250.0
100-101	4785265.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.55920696735251
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.79684891268847	76.82947181136797
2	7.092272406063511	12.136184053184623
3	1.7695182296927876	4.5419572934036525
4	0.6362302121815292	2.1774140961168826
5	0.30112423113973785	1.2881975207484864
6	0.1529468630788318	0.7851607387901458
7	0.0822156674164074	0.49240151131075954
8	0.04713414117070717	0.32262077917223564
9	0.03085783015064883	0.2376154328780496
>10	0.09002623490934214	1.1086475599988848
>50	5.579787150928278E-4	0.02928814446917083
>100	2.442265802543694E-4	0.03819339867313428
>500	2.306621267490718E-5	0.01284765988597685
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.2889617696517045E-5	0.0	0.0	0.0
5	0.0	1.2889617696517045E-5	0.0	0.0	0.0
6	0.0	1.2889617696517045E-5	0.0	0.0	0.0
7	0.0	1.2889617696517045E-5	0.0	1.2889617696517045E-5	0.0
8	0.0	2.577923539303409E-5	0.0	2.577923539303409E-5	0.0
9	0.0	5.155847078606818E-5	0.0	2.577923539303409E-5	0.0
10-11	0.0	5.155847078606818E-5	0.0	3.866885308955113E-5	0.0
12-13	0.0	5.155847078606818E-5	0.0	4.511366193780966E-5	0.0
14-15	0.0	5.80032796343267E-5	0.0	5.155847078606818E-5	2.577923539303409E-5
16-17	0.0	7.089289733084374E-5	0.0	5.155847078606818E-5	2.577923539303409E-5
18-19	0.0	8.378251502736078E-5	0.0	7.089289733084374E-5	2.577923539303409E-5
20-21	0.0	9.022732387561932E-5	0.0	1.2245136811691194E-4	2.577923539303409E-5
22-23	0.0	9.667213272387784E-5	0.0	1.8689945659949714E-4	3.866885308955114E-5
24-25	0.0	1.0311694157213636E-4	0.0	2.8357158932337497E-4	5.155847078606818E-5
26-27	0.0	1.0311694157213636E-4	0.0	5.864776051915255E-4	6.444808848258522E-5
28-29	0.0	1.0311694157213636E-4	0.0	0.0031772907621914516	6.444808848258522E-5
30-31	0.0	1.160065592686534E-4	0.0	0.011420201279114101	6.444808848258522E-5
32-33	0.0	1.2245136811691194E-4	0.0	0.032327161182864744	7.089289733084374E-5
34-35	0.0	1.4823060350994602E-4	0.0	0.054084835854585525	7.733770617910226E-5
36-37	0.0	1.8045464775123864E-4	0.0	0.0807663444863758	7.733770617910226E-5
38-39	6.4448088482585225E-6	2.0623388314427272E-4	0.0	0.10873681488781779	7.733770617910226E-5
40-41	1.2889617696517045E-5	2.577923539303409E-4	0.0	0.1322732568016579	7.733770617910226E-5
42-43	1.2889617696517045E-5	2.706819716268579E-4	0.0	0.15553901674387116	7.733770617910226E-5
44-45	2.577923539303409E-5	3.029060158681506E-4	0.0	0.176574872824587	7.733770617910226E-5
46-47	2.577923539303409E-5	3.2868525126118466E-4	0.0	0.1984549988644247	7.733770617910226E-5
48-49	2.577923539303409E-5	3.480196778059602E-4	0.0	0.21546284941497892	7.733770617910226E-5
50-51	2.577923539303409E-5	3.9957814859202837E-4	0.0	0.22948675346878947	7.733770617910226E-5
52-53	2.577923539303409E-5	3.9957814859202837E-4	0.0	0.24113896786644087	1.0311694157213636E-4
54-55	2.577923539303409E-5	3.9957814859202837E-4	0.0	0.2518953538341844	1.3534098581342895E-4
56-57	2.577923539303409E-5	3.9957814859202837E-4	0.0	0.26277419117004475	1.4178579466168749E-4
58-59	2.577923539303409E-5	4.1246776628854544E-4	0.0	0.27254452138400465	1.675650300547216E-4
60-61	2.577923539303409E-5	4.1246776628854544E-4	0.0	0.2818637149785865	1.675650300547216E-4
62-63	2.577923539303409E-5	4.31802192833321E-4	0.0	0.29054487249719074	1.675650300547216E-4
64-65	2.577923539303409E-5	5.026950901641647E-4	0.0	0.2978790649665089	1.675650300547216E-4
66-67	2.577923539303409E-5	5.026950901641647E-4	0.0	0.30501991317037935	1.675650300547216E-4
68-69	2.577923539303409E-5	5.155847078606818E-4	0.0	0.3119609722999538	1.675650300547216E-4
70-71	2.577923539303409E-5	5.478087521019744E-4	0.0	0.3181931024562198	1.675650300547216E-4
72-73	2.577923539303409E-5	5.54253560950233E-4	0.0	0.32450257031866486	1.675650300547216E-4
74-75	2.577923539303409E-5	5.54253560950233E-4	0.0	0.33014177806089107	1.8045464775123864E-4
76-77	2.577923539303409E-5	5.54253560950233E-4	0.0	0.33415044916450787	1.8689945659949717E-4
78-79	2.577923539303409E-5	5.54253560950233E-4	0.0	0.33656080767375657	1.9334426544775568E-4
80-81	2.577923539303409E-5	5.54253560950233E-4	0.0	0.33759842189832623	2.0623388314427272E-4
82-83	2.577923539303409E-5	5.54253560950233E-4	0.0	0.3384620262839928	2.1912350084078976E-4
84-85	2.577923539303409E-5	5.80032796343267E-4	0.0	0.338790711535254	2.1912350084078976E-4
86-87	2.577923539303409E-5	6.058120317363011E-4	0.0	0.3388809388591296	2.1912350084078976E-4
88	2.577923539303409E-5	6.315912671293352E-4	0.0	0.33889382847682614	2.320131185373068E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6465	0.0	38.365543	1
GTATCAA	10800	0.0	30.764357	1
TCAACGC	11685	0.0	26.953444	4
ATCAACG	11770	0.0	26.616726	3
CAACGCA	12015	0.0	26.142218	5
AACGCAG	12500	0.0	25.195105	6
TATCAAC	13155	0.0	24.297384	2
ACGCAGA	15050	0.0	20.78776	7
CGCAGAG	15230	0.0	20.514559	8
GTACATG	23470	0.0	19.34195	1
TACATGG	22980	0.0	19.278547	2
ACATGGG	23765	0.0	18.267496	3
CATGGGG	17565	0.0	16.612175	4
GCAGAGT	18995	0.0	16.293194	9
AGAGTAC	16640	0.0	14.993016	10-11
TATACCG	535	0.0	14.952961	5
CAGAGTA	18900	0.0	14.570348	10-11
GTGTAGC	4115	0.0	13.405901	1
ACCGTGC	1100	0.0	13.397802	8
GTATAAT	4420	0.0	13.14775	1
>>END_MODULE