FastQCFastQC Report
Fri 27 May 2016
SRR2140325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23440650
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182600.037.3967481
GTATCAA314900.030.6689641
TCAACGC324950.027.9287954
ATCAACG330900.027.4538153
CAACGCA334350.027.0545795
AACGCAG348600.025.9733686
TATCAAC370000.025.0706732
ACGCAGA423350.021.408947
CGCAGAG444100.020.4272568
GTACATG696650.019.6103251
TACATGG692250.019.1778682
ACATGGG718850.017.9473763
CATGGGG526100.016.4443554
GCAGAGT556450.016.2419519
AGAGTAC477900.014.98432210-11
ACCGAAC29700.014.8510418
CAGAGTA541100.014.33146110-11
GGACCGA36050.013.1756036
GTGTAGC133300.013.0773711
ATGGGGG321350.012.9461915
Produced by FastQC (version 0.11.5)