##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140325_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23440650
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04640046244452	33.0	31.0	34.0	30.0	34.0
2	32.08132995458744	34.0	31.0	34.0	30.0	34.0
3	32.29104077745284	34.0	31.0	34.0	30.0	34.0
4	35.71351037620544	37.0	35.0	37.0	35.0	37.0
5	35.78668663198333	37.0	35.0	37.0	35.0	37.0
6	35.843534671606804	37.0	36.0	37.0	35.0	37.0
7	35.75123236770311	37.0	36.0	37.0	35.0	37.0
8	35.70846119881488	37.0	36.0	37.0	35.0	37.0
9	37.42265615501277	39.0	38.0	39.0	35.0	39.0
10-11	37.42536105014153	39.0	38.0	39.0	35.0	39.0
12-13	37.36681218310926	39.0	38.0	39.0	35.0	39.0
14-15	38.774048159927304	41.0	39.0	41.0	35.0	41.0
16-17	38.78094572889404	41.0	39.0	41.0	35.0	41.0
18-19	38.79133714295465	40.5	38.5	41.0	34.0	41.0
20-21	38.83755299337634	40.5	39.0	41.0	34.0	41.0
22-23	38.79724402572057	40.0	39.0	41.0	34.0	41.0
24-25	38.765095608959044	40.0	38.0	41.0	34.0	41.0
26-27	38.716508965632045	40.0	38.0	41.0	34.0	41.0
28-29	38.64691757402852	40.0	38.0	41.0	34.0	41.0
30-31	38.53226348299809	40.0	38.0	41.0	34.0	41.0
32-33	38.480080757942744	40.0	38.0	41.0	34.0	41.0
34-35	38.333882500396875	40.0	38.0	41.0	33.0	41.0
36-37	38.21131181759034	40.0	38.0	41.0	33.0	41.0
38-39	38.194162173246426	40.0	38.0	41.0	33.0	41.0
40-41	38.108977883945684	40.0	38.0	41.0	33.0	41.0
42-43	37.925584196169254	40.0	37.5	41.0	33.0	41.0
44-45	37.97578180651804	40.0	37.5	41.0	32.5	41.0
46-47	38.18366366368015	40.0	38.0	41.0	33.0	41.0
48-49	38.17189370997955	40.0	38.0	41.0	33.0	41.0
50-51	38.07361197908445	40.0	37.5	41.0	33.0	41.0
52-53	37.99715417453618	40.0	37.0	41.0	33.0	41.0
54-55	37.91382098025588	40.0	37.0	41.0	33.0	41.0
56-57	37.964953818011	40.0	37.0	41.0	33.0	41.0
58-59	37.76509792302119	40.0	36.5	41.0	32.0	41.0
60-61	37.67520487645592	39.5	36.0	41.0	32.0	41.0
62-63	37.444373148858446	39.0	35.5	41.0	32.0	41.0
64-65	37.23269580731208	39.0	35.0	41.0	32.0	41.0
66-67	36.95500323780465	38.5	35.0	41.0	31.0	41.0
68-69	36.7170251722649	38.0	35.0	40.0	31.0	41.0
70-71	36.42622459876575	37.0	35.0	40.0	31.0	41.0
72-73	36.098389713189235	37.0	35.0	39.0	31.0	41.0
74-75	35.763270534235424	36.0	34.5	39.0	31.0	41.0
76-77	35.43714811092459	36.0	34.0	39.0	31.0	40.5
78-79	35.08892846939095	35.0	34.0	37.0	30.0	39.5
80-81	34.78077849441949	35.0	34.0	37.0	30.0	39.0
82-83	34.48094268302837	35.0	34.0	36.5	30.0	39.0
84-85	34.265771976059185	35.0	34.0	36.0	30.0	37.5
86-87	34.046689349585094	35.0	34.0	36.0	30.0	37.0
88-89	33.817660460029245	35.0	34.0	35.5	30.0	37.0
90-91	33.65412512119574	35.0	34.0	35.0	29.5	36.0
92-93	33.56179743955093	35.0	34.0	35.0	29.5	36.0
94-95	33.538333544351	35.0	34.0	35.0	30.0	36.0
96-97	33.49417995650738	35.0	33.5	35.0	30.0	36.0
98-99	33.52839711246525	35.0	33.0	35.0	30.0	35.5
100	32.9259595148563	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	4.0
15	20.0
16	135.0
17	789.0
18	3686.0
19	10553.0
20	23714.0
21	42075.0
22	65339.0
23	91594.0
24	121178.0
25	155424.0
26	184018.0
27	199917.0
28	227431.0
29	274967.0
30	324601.0
31	390833.0
32	507225.0
33	683970.0
34	977444.0
35	1470429.0
36	2429051.0
37	4396619.0
38	6426557.0
39	4176609.0
40	256462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.59096057489873	20.825177629459933	14.732027482173063	23.85183431346827
2	14.192786545339398	21.210056492339188	41.82958321426574	22.767573748055682
3	17.808834450751768	26.319818527912393	30.41373509481794	25.457611926517902
4	12.335875388783968	18.53547554119381	39.40912644167855	29.719522628343665
5	13.434049029891737	38.252047335074685	35.37948090404009	12.934422730993486
6	29.379746997461442	38.13928365723063	19.43978496917465	13.041184376133286
7	26.342741886628758	32.89126750622484	23.345282165301732	17.420708441844663
8	24.0056607645616	36.97603475985909	21.553638389438245	17.46466608614107
9	24.96569141269296	18.05025685950965	21.66090636787944	35.32314535991795
10-11	23.709792281385102	27.39363933401629	29.14633840679913	19.750229977799478
12-13	24.35861534566899	24.852814458894038	29.077721325380985	21.710848870055983
14-15	21.809532484326162	26.417797963995447	26.465335169831654	25.30733438184674
16-17	21.699804799443477	29.346148635574583	27.162284681631583	21.791761883350365
18-19	21.77026456433668	28.9632035936868	28.237299646017995	21.029232195958528
20-21	22.70116316861985	28.06488190547935	27.94243378042745	21.291521145473343
22-23	22.42622745794054	27.980311646200445	27.696195260589374	21.897265635269633
24-25	22.170728106366404	28.12690078771849	27.76573455777082	21.936636548144286
26-27	22.142453191990892	28.24171395365365	27.66240067208231	21.953432182273144
28-29	22.057178367133528	28.296038276377317	27.72949090283215	21.91729245365701
30-31	22.352411151607026	28.122619499234357	27.637659747920136	21.887309601238485
32-33	22.13589175685631	28.199156008086085	27.642085815465343	22.02286641959226
34-35	22.290942601130563	28.22200506818368	27.64614630040671	21.84090603027905
36-37	22.259374961001576	28.056293444243696	27.65704058470596	22.027291010048767
38-39	22.04404770297805	28.182121570663266	27.815267257131048	21.958563469227638
40-41	22.301291276632604	28.021931153420766	27.7516096685666	21.925167901380032
42-43	22.19246712508258	28.06691926858951	27.646398936498368	22.094214669829547
44-45	22.21817416960269	28.123403026590555	27.621922571602465	22.03650023220429
46-47	22.211211179166156	28.063106296676825	27.570361001364212	22.155321522792804
48-49	22.084986051561653	28.08318202046973	27.727685091290468	22.104146836678147
50-51	22.10216339004705	28.092892010530612	27.699636766911524	22.105307832510817
52-53	22.180838694728063	28.026699830464842	27.608417870083663	22.18404360472343
54-55	22.200287141338986	27.965709738859772	27.691982359109062	22.142020760692173
56-57	22.1926357853573	28.040624232253187	27.65121117595871	22.115528806430802
58-59	22.179881080250333	28.10774876100887	27.66362918196433	22.048740976776465
60-61	22.19356651859802	27.990321036316644	27.730542005797204	22.085570439288134
62-63	22.21929663559984	28.087891554654927	27.62445853787187	22.068353271873367
64-65	22.166518634020253	28.043387739806914	27.652048534365754	22.138045091807076
66-67	22.20484125568058	28.04293989234071	27.57887978565641	22.1733390663223
68-69	22.18451579710779	27.9746745186374	27.655080065913694	22.185729618341117
70-71	22.124428882365592	27.82851518580744	27.723220434002023	22.323835497824945
72-73	22.18720970190386	27.87670754293337	27.674512520300215	22.261570234862553
74-75	22.034722072066447	28.02873418123719	27.669360232856334	22.26718351384003
76-77	22.14790237489112	27.983092521828272	27.618512392946887	22.25049271033372
78-79	22.115849117251376	27.971196388341458	27.589960981603834	22.32299351280333
80-81	22.057050768436802	27.96791646387402	27.692505780573935	22.28252698711524
82-83	22.112503568952427	27.997575778866075	27.738310836739156	22.151609815442345
84-85	22.214560121233692	27.980852978650706	27.650737566688864	22.153849333426738
86-87	22.061720878329425	28.037409211000003	27.715335031193295	22.185534879477277
88-89	22.14534769295097	28.002882534988295	27.682350362116114	22.169419409944624
90-91	22.1197264495516	28.07067645839262	27.705191718920435	22.104405373135346
92-93	22.144644920848553	28.07315845963973	27.716582387982363	22.065614231529356
94-95	21.951177300999163	28.139497694675637	27.791366976614242	22.117958027710966
96-97	22.027037200951746	28.295306487646872	27.82801605060706	21.849640260794317
98-99	23.128867714106516	30.193487379489614	28.018611158548346	18.659033747855528
100	22.113359187608307	31.24854160998676	21.750192573483734	24.887906628921204
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7610.0
1	6033.5
2	4850.5
3	5195.5
4	5031.0
5	5268.5
6	6292.5
7	7483.5
8	8437.5
9	9552.5
10	10649.0
11	11342.5
12	12175.5
13	13538.0
14	15526.0
15	18317.5
16	21731.5
17	26022.0
18	31555.5
19	38678.0
20	47885.0
21	60366.0
22	77677.0
23	100434.5
24	129962.0
25	168603.5
26	218499.5
27	274222.5
28	329948.5
29	389537.5
30	444003.5
31	495452.5
32	549360.0
33	603767.0
34	661357.5
35	713246.5
36	764448.5
37	810713.0
38	844389.0
39	875223.5
40	907444.0
41	930935.5
42	944862.5
43	960063.5
44	972868.5
45	985854.5
46	992887.5
47	990449.0
48	984392.0
49	969727.5
50	947363.5
51	918296.5
52	879930.5
53	831340.5
54	778554.5
55	722378.5
56	662802.5
57	597929.0
58	531892.0
59	465583.0
60	393548.5
61	323611.5
62	259485.0
63	204171.0
64	158005.0
65	120888.5
66	93177.0
67	73498.0
68	57934.0
69	44388.0
70	34521.5
71	26865.0
72	20875.5
73	16055.5
74	12275.5
75	9610.0
76	7406.5
77	5717.5
78	4427.5
79	3351.0
80	2547.5
81	1937.0
82	1500.0
83	1100.5
84	822.5
85	638.0
86	532.5
87	435.5
88	289.0
89	194.5
90	151.0
91	122.0
92	102.0
93	64.5
94	35.0
95	25.0
96	23.5
97	25.0
98	19.5
99	33.0
100	111.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.021684552262842542
3	0.01278121553796503
4	0.005200367737242782
5	0.009095310923545208
6	0.009172100603012288
7	0.002994797499216105
8	0.009044117803900489
9	0.005046788378308622
10-11	0.0037861578070573984
12-13	0.009050516943856078
14-15	0.00475669403365521
16-17	0.0038885440463468377
18-19	0.005644041440830353
20-21	0.004398335265314369
22-23	0.010122115205174528
24-25	0.009454155849451203
26-27	0.02267472570180479
28-29	0.019079888542614727
30-31	0.015599212471441665
32-33	0.012435907391262097
34-35	0.009062605388382257
36-37	0.010340012675343424
38-39	0.005315657233524578
40-41	0.0061100726520073425
42-43	0.004142831513555823
44-45	0.007226028781705533
46-47	0.009900457454372279
48-49	0.007098838579060942
50-51	0.010043485497931635
52-53	9.614637543960069E-4
54-55	0.0028013229536087768
56-57	6.541154253941901E-4
58-59	0.002807280718957043
60-61	0.001019446801223687
62-63	0.007253099886859297
64-65	0.0037536834376575698
66-67	0.004026580108518771
68-69	0.0051824772393847295
70-71	7.884669913392994E-4
72-73	4.924514355574911E-4
74-75	0.0
76-77	1.5387778559505423E-5
78-79	4.2266249940457745E-4
80-81	1.757329157639481E-4
82-83	0.0
84-85	9.420967224132942E-4
86-87	0.0035552926760726057
88-89	0.021892383114331792
90-91	0.002811255966785892
92-93	0.0013764267869794226
94-95	0.0012114466478607843
96-97	4.605774353784763E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	7759.0
20-21	14688.0
22-23	16523.0
24-25	19718.0
26-27	24967.0
28-29	34641.0
30-31	55610.0
32-33	68421.0
34-35	89290.0
36-37	123615.0
38-39	137270.0
40-41	135564.0
42-43	148562.0
44-45	168089.0
46-47	173295.0
48-49	171294.0
50-51	167694.0
52-53	169916.0
54-55	185902.0
56-57	194872.0
58-59	194821.0
60-61	190262.0
62-63	186577.0
64-65	196209.0
66-67	204610.0
68-69	206605.0
70-71	204212.0
72-73	197897.0
74-75	203017.0
76-77	211907.0
78-79	218172.0
80-81	219976.0
82-83	215201.0
84-85	224399.0
86-87	276140.0
88-89	245406.0
90-91	254035.0
92-93	272611.0
94-95	340835.0
96-97	822055.0
98-99	1475730.0
100-101	1.4772283E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.95294751139419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.49038210870175	72.5204764021765
2	7.466525178640579	12.23807492115269
3	2.091751856264985	5.142756902500372
4	0.8316141564219877	2.7261292524393403
5	0.39856925953872707	1.633196280331627
6	0.23315734309448036	1.1464758900310845
7	0.1381121780609855	0.7923090055521392
8	0.09401898310769151	0.616410623015944
9	0.05380855770373146	0.3968792914641957
>10	0.1985944649461386	2.4654268503937082
>50	0.002549970736080396	0.1395573961072032
>100	8.550584895712923E-4	0.11184018758780385
>500	3.0511369892130034E-5	0.0175076683672526
>1k	3.0372923391198973E-5	0.05295932888015367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	4.2660933037266455E-6	0.0	0.0	0.0
2	0.0	2.5596559822359876E-5	0.0	4.2660933037266455E-6	0.0
3	0.0	2.5596559822359876E-5	0.0	4.2660933037266455E-6	0.0
4	0.0	8.958795937825956E-5	0.0	8.532186607453291E-6	0.0
5	0.0	9.385405268198621E-5	0.0	1.7064373214906582E-5	0.0
6	0.0	9.385405268198621E-5	0.0	2.1330466518633227E-5	0.0
7	0.0	9.385405268198621E-5	0.0	2.1330466518633227E-5	0.0
8	0.0	1.1518451920061944E-4	0.0	3.4128746429813164E-5	0.0
9	0.0	2.3036903840123888E-4	0.0	3.839483973353981E-5	0.0
10-11	4.2660933037266455E-6	2.3036903840123888E-4	0.0	4.906007299285642E-5	0.0
12-13	4.2660933037266455E-6	2.3463513170496553E-4	0.0	6.185835290403636E-5	0.0
14-15	6.399139955589968E-6	3.348883243425417E-4	0.0	6.825749285962633E-5	0.0
16-17	8.532186607453291E-6	3.4768660425372165E-4	0.0	8.105577277080626E-5	0.0
18-19	8.532186607453291E-6	3.754162107279448E-4	0.0	9.38540526819862E-5	0.0
20-21	8.532186607453291E-6	4.09544957157758E-4	0.0	1.1945061250434607E-4	0.0
22-23	1.2798279911179938E-5	4.4367370358757113E-4	0.0	1.5357935893415924E-4	0.0
24-25	1.2798279911179938E-5	4.927337765804275E-4	0.0	2.4103427166055548E-4	0.0
26-27	1.2798279911179938E-5	5.161972897509241E-4	0.0	4.287423770245279E-4	0.0
28-29	1.2798279911179938E-5	5.183303364027875E-4	0.0	0.0018130896540838244	0.0
30-31	1.2798279911179938E-5	5.95120015869867E-4	0.0	0.007109444490660455	0.0
32-33	1.2798279911179938E-5	5.972530625217304E-4	0.0	0.0189243898953314	0.0
34-35	1.2798279911179938E-5	6.847079752481267E-4	0.0	0.029039297118467276	0.0
36-37	1.2798279911179938E-5	7.742959346263862E-4	0.0	0.04263107038414037	0.0
38-39	1.2798279911179938E-5	8.148238210117894E-4	0.0	0.05693954732483954	0.0
40-41	1.2798279911179938E-5	8.958795937825956E-4	0.0	0.06987860831504246	0.0
42-43	1.2798279911179938E-5	9.321413868642721E-4	0.0	0.0823228024820131	0.0
44-45	1.2798279911179938E-5	0.001036660672805575	0.0	0.09462621556996073	0.0
46-47	1.7064373214906582E-5	0.001109184258968928	0.0	0.10840143084769407	0.0
48-49	1.7064373214906582E-5	0.001109184258968928	0.0	0.11894934654115821	0.0
50-51	1.7064373214906582E-5	0.0012329009647770008	0.0	0.12845420242186117	0.0
52-53	1.7064373214906582E-5	0.001241433151384454	0.0	0.1376284360715253	0.0
54-55	1.7064373214906582E-5	0.001247832291340044	0.0	0.14602624073991122	0.0
56-57	1.7064373214906582E-5	0.001262763617903087	4.2660933037266455E-6	0.15460962046700924	0.0
58-59	1.7064373214906582E-5	0.0012648966645549503	4.2660933037266455E-6	0.16248056261238492	0.0
60-61	1.7064373214906582E-5	0.0012904932243773103	4.2660933037266455E-6	0.16985877098118013	0.0
62-63	1.7064373214906582E-5	0.001307557597592217	4.2660933037266455E-6	0.17709406522430052	0.0
64-65	1.7064373214906582E-5	0.0014846004696968727	4.2660933037266455E-6	0.18352093478636472	0.0
66-67	1.7064373214906582E-5	0.0015016648429117794	4.2660933037266455E-6	0.18981555545601336	0.0
68-69	1.7064373214906582E-5	0.001518729216126686	4.2660933037266455E-6	0.19589260536717198	0.0
70-71	2.1330466518633227E-5	0.0015379266359934558	4.2660933037266455E-6	0.20126148378991196	0.0
72-73	2.1330466518633227E-5	0.0015549910092083623	4.2660933037266455E-6	0.20611416492290102	0.0
74-75	2.1330466518633227E-5	0.0015976519422456288	4.2660933037266455E-6	0.21065968733802176	0.0
76-77	2.1330466518633227E-5	0.0016723085750608453	4.2660933037266455E-6	0.21404483237452887	0.0
78-79	2.1330466518633227E-5	0.0017064373214906584	4.2660933037266455E-6	0.21606482755384343	0.0
80-81	2.1330466518633227E-5	0.001710703414794385	4.2660933037266455E-6	0.2170801577601304	0.0
82-83	2.1330466518633227E-5	0.0017213686480537014	4.2660933037266455E-6	0.217882183301231	0.0
84-85	2.1330466518633227E-5	0.0017533643478316514	4.2660933037266455E-6	0.21813601585280273	2.1330466518633227E-6
86-87	2.1330466518633227E-5	0.0017874930942614645	4.2660933037266455E-6	0.21822560381218098	4.2660933037266455E-6
88	2.1330466518633227E-5	0.001889879333550904	4.2660933037266455E-6	0.21823200295213657	4.2660933037266455E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18260	0.0	37.396748	1
GTATCAA	31490	0.0	30.668964	1
TCAACGC	32495	0.0	27.928795	4
ATCAACG	33090	0.0	27.453815	3
CAACGCA	33435	0.0	27.054579	5
AACGCAG	34860	0.0	25.973368	6
TATCAAC	37000	0.0	25.070673	2
ACGCAGA	42335	0.0	21.40894	7
CGCAGAG	44410	0.0	20.427256	8
GTACATG	69665	0.0	19.610325	1
TACATGG	69225	0.0	19.177868	2
ACATGGG	71885	0.0	17.947376	3
CATGGGG	52610	0.0	16.444355	4
GCAGAGT	55645	0.0	16.241951	9
AGAGTAC	47790	0.0	14.984322	10-11
ACCGAAC	2970	0.0	14.851041	8
CAGAGTA	54110	0.0	14.331461	10-11
GGACCGA	3605	0.0	13.175603	6
GTGTAGC	13330	0.0	13.077371	1
ATGGGGG	32135	0.0	12.946191	5
>>END_MODULE