FastQCFastQC Report
Fri 27 May 2016
SRR2140324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6727803
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.033.0958861
GTATCAA86750.031.0585631
TCAACGC85100.029.6819614
CAACGCA85700.029.4741545
ATCAACG85650.029.4409473
AACGCAG91600.027.5757066
TATCAAC98850.026.4705052
CGCAGAG111700.022.4975938
ACGCAGA111950.022.4473537
TACATGG187450.021.4912872
TGTAGAT93050.021.44033894
GTACATG193650.021.4052351
ACATGGG190300.020.3526023
GGTGTAG105750.019.78127194
GTGTAGA96400.019.02089194
CATGGGG143650.018.4856934
AGGTGTA130050.016.92908994
GCAGAGT149150.016.6460369
TTAGGTG166500.015.66594894
TAGGTGT140700.015.55590194
Produced by FastQC (version 0.11.5)