##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140324_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6727803
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.821824152698884	33.0	31.0	34.0	30.0	34.0
2	31.92679140575311	33.0	31.0	34.0	30.0	34.0
3	31.983882108319758	34.0	31.0	34.0	30.0	34.0
4	35.49272563420778	37.0	35.0	37.0	33.0	37.0
5	35.551259601388445	37.0	35.0	37.0	33.0	37.0
6	35.60451933565831	37.0	35.0	37.0	33.0	37.0
7	35.50757401784803	37.0	35.0	37.0	33.0	37.0
8	35.47954332194328	37.0	35.0	37.0	33.0	37.0
9	37.092079390552904	39.0	37.0	39.0	33.0	39.0
10-11	37.06578113241425	39.0	37.0	39.0	33.0	39.0
12-13	37.01444646937492	39.0	37.0	39.0	33.0	39.0
14-15	38.37414138018013	40.0	38.0	41.0	33.0	41.0
16-17	38.38324472342606	40.0	38.0	41.0	33.0	41.0
18-19	38.39876160166996	40.0	38.0	41.0	33.0	41.0
20-21	38.4478771927541	40.0	38.0	41.0	33.0	41.0
22-23	38.42641007031903	40.0	38.0	41.0	33.0	41.0
24-25	38.43561593244555	40.0	38.0	41.0	33.0	41.0
26-27	38.40748674786887	40.0	38.0	41.0	33.0	41.0
28-29	38.378230000776036	40.0	38.0	41.0	33.0	41.0
30-31	38.32854913836755	40.0	38.0	41.0	33.0	41.0
32-33	38.28646166613281	40.0	38.0	41.0	33.0	41.0
34-35	38.23171114755381	40.0	38.0	41.0	33.0	41.0
36-37	38.17558781289661	40.0	38.0	41.0	33.0	41.0
38-39	38.09780894945236	40.0	38.0	41.0	33.0	41.0
40-41	38.03347072817565	40.0	38.0	41.0	32.5	41.0
42-43	37.95253351381509	40.0	37.0	41.0	32.0	41.0
44-45	37.84367953652027	40.0	37.0	41.0	32.0	41.0
46-47	37.74479172728337	40.0	37.0	41.0	32.0	41.0
48-49	37.64633972280676	40.0	37.0	41.0	31.5	41.0
50-51	37.516808054284745	40.0	36.5	41.0	31.0	41.0
52-53	37.39781460293334	40.0	36.0	41.0	31.0	41.0
54-55	37.25968250522024	39.0	36.0	41.0	31.0	41.0
56-57	37.084411560408476	39.0	35.5	41.0	31.0	41.0
58-59	37.23478961414683	39.0	35.5	41.0	31.0	41.0
60-61	37.301362213585364	39.0	35.5	41.0	31.0	41.0
62-63	37.145573956567716	39.0	35.0	41.0	31.0	41.0
64-65	36.94698810804534	39.0	35.0	41.0	31.0	41.0
66-67	36.6877841919255	38.0	35.0	40.0	31.0	41.0
68-69	36.42359422288354	37.5	35.0	40.0	31.0	41.0
70-71	36.193157328315294	37.0	35.0	40.0	31.0	41.0
72-73	35.96503580412474	36.5	35.0	39.0	31.0	41.0
74-75	35.67630044621542	36.0	34.0	39.0	30.5	41.0
76-77	35.41331956535568	36.0	34.0	39.0	30.0	40.5
78-79	35.090415634456974	35.0	34.0	37.5	30.0	39.5
80-81	34.77961703302822	35.0	34.0	37.0	30.0	39.0
82-83	34.49100776986947	35.0	34.0	36.5	30.0	39.0
84-85	34.246436479114706	35.0	34.0	36.0	30.0	37.5
86-87	34.0575859082234	35.0	34.0	36.0	30.0	37.0
88-89	33.93796743026083	35.0	34.0	35.5	30.0	37.0
90-91	33.82271162246065	35.0	34.0	35.0	30.0	36.0
92-93	33.72281978069633	35.0	34.0	35.0	30.0	36.0
94-95	33.681610213898416	35.0	34.0	35.0	30.0	36.0
96-97	33.674968051097395	35.0	34.0	35.0	30.0	36.0
98-99	33.702987050934894	35.0	34.0	35.0	30.0	36.0
100	33.35046532421967	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	22.0
17	224.0
18	1016.0
19	3240.0
20	7660.0
21	15404.0
22	24594.0
23	34365.0
24	44719.0
25	55936.0
26	65356.0
27	72591.0
28	82528.0
29	100762.0
30	119110.0
31	139867.0
32	173024.0
33	223389.0
34	299976.0
35	419414.0
36	641849.0
37	1185021.0
38	1828813.0
39	1127099.0
40	61823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.24536642942726	20.528068375367113	14.092876976332391	24.133688218873235
2	14.010888844396902	20.96703782795067	42.26410910069751	22.757964226954922
3	17.81333371384388	26.24094373750242	30.40268866374357	25.543033884910127
4	12.101617719781627	17.983002772227426	40.02217365758183	29.893205850409117
5	13.110016711290056	38.18969001248107	36.00799192958992	12.692301346638946
6	29.405082164266698	38.2687780840194	19.532349564932268	12.793790186781628
7	26.35780209378901	32.711466135378814	23.600111358789785	17.330620412042386
8	23.961778304415024	37.22362816265996	21.546680476030655	17.26791305689436
9	25.1108868675257	17.571560879532296	21.73990528557391	35.577646967368096
10-11	23.729041805357415	27.104856596467375	29.51885594662622	19.647245651548992
12-13	24.40627114594338	24.596162414222487	29.42897731389805	21.56858912593609
14-15	21.713779754054872	26.261899968362624	26.6403445464317	25.38397573115081
16-17	21.653045694955683	29.31959402178974	27.26621832294497	21.761141960309608
18-19	21.69666938500924	28.998440266409958	28.326625916548792	20.97826443203201
20-21	22.575857980844333	28.073379200972713	28.056091094416708	21.294671723766246
22-23	22.323399819259873	27.959939535023885	27.819774785450157	21.89688586026608
24-25	22.05783017439722	28.14369353473219	27.88394855558603	21.91452773528456
26-27	22.039494279082053	28.271803676723568	27.757915342697565	21.930786701496817
28-29	22.016675769791124	28.30307038848265	27.798136156722297	21.882117685003934
30-31	22.22057158698684	28.124668699564765	27.779705041867402	21.87505467158099
32-33	21.99715651236031	28.236974694669158	27.788817294449846	21.977051498520687
34-35	22.165044828554056	28.187132251078413	27.840513051768156	21.807309868599376
36-37	22.137122739116165	28.0517394987467	27.858326318083332	21.952811444053808
38-39	22.01268524584608	28.148264614808937	27.973510583995047	21.865539555349937
40-41	22.20871459451712	27.999344087614475	27.899626437399185	21.892314880469222
42-43	21.98170442265701	28.03556731384261	27.904417517802965	22.07831074569741
44-45	22.078961172290192	28.073217874127764	27.898164921829483	21.94965603175256
46-47	21.95294758153328	28.014420147760795	27.831804265359615	22.20082800534631
48-49	21.900186242349076	28.07172485075606	27.91111009058594	22.11697881630893
50-51	21.806909604426476	27.993060892673682	27.989947838967893	22.210081663931945
52-53	21.962215776293856	27.870801421535678	27.90784353468479	22.259139267485672
54-55	21.97235960651839	27.616517093175563	28.059671438027156	22.351451862278896
56-57	21.8373719177703	27.482415438525937	28.203479496980364	22.476733146723394
58-59	21.671951827820195	27.52490099235895	28.39892640176408	22.40422077805677
60-61	21.676422085297347	27.344133564960682	28.659902037209772	22.3195423125322
62-63	21.533870476861715	27.57582213394416	28.61372919636707	22.276578192827053
64-65	21.329310076526014	27.877999202695776	28.310868820831647	22.48182189994656
66-67	21.204387384621707	28.10689070980669	28.070980016918725	22.617741888652873
68-69	21.324596884973253	28.21007347457527	28.10971948415314	22.355610156298333
70-71	21.33086301122356	28.28386211481355	27.7730057831803	22.61226909078259
72-73	21.706486024541096	28.155892100953306	27.355430620871278	22.782191253634316
74-75	21.908958200948376	27.976741846466137	27.444341012615997	22.669958939969497
76-77	22.15031788308716	27.535547104899276	27.46459098207974	22.849544029933828
78-79	22.404239875345112	27.356734793813448	27.286827969388387	22.95219736145305
80-81	22.790280958337934	27.335197053658256	27.063013101428375	22.811508886575435
82-83	22.456847151581183	27.649583739914117	26.982004842250795	22.911564266253905
84-85	22.91087611951824	27.66509264531641	26.90032113909986	22.523710096065496
86-87	22.71555308970254	27.906710926228577	26.950622381946275	22.4271136021226
88-89	22.633597374936507	28.159280370018188	26.807283787243218	22.399838467802095
90-91	22.806263698235092	28.25820777780672	27.190619895139896	21.744908628818298
92-93	22.994425054240168	27.996802188472785	27.467220073577963	21.541552683709085
94-95	22.647108362403603	28.017040088484823	27.907468209744113	21.428383339367464
96-97	22.853379057848734	27.770898595421713	28.149558811339848	21.226163535389713
98-99	23.615720220675325	29.813850861492124	28.587046173385726	17.98338274444682
100	22.81193574075054	30.998471817550044	22.44240637381468	23.747186067884737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1572.0
1	1207.0
2	928.0
3	993.0
4	985.5
5	1092.5
6	1290.0
7	1512.5
8	1742.0
9	2012.0
10	2298.5
11	2503.0
12	2728.5
13	3104.5
14	3655.5
15	4381.5
16	5317.5
17	6464.5
18	7775.0
19	9472.5
20	11940.0
21	15295.0
22	19757.5
23	25697.0
24	33840.5
25	44491.0
26	57677.0
27	73047.0
28	89206.5
29	106498.5
30	122720.0
31	138343.0
32	154595.5
33	168990.0
34	182774.5
35	196423.0
36	211734.0
37	227450.0
38	237690.5
39	244305.5
40	253317.0
41	261994.0
42	267877.0
43	274342.0
44	280332.0
45	285741.0
46	288286.5
47	287441.5
48	284926.5
49	280037.5
50	272402.0
51	264467.0
52	254338.5
53	238325.5
54	221776.0
55	205089.5
56	187633.5
57	168773.0
58	149619.5
59	130671.5
60	108316.5
61	86537.5
62	68446.0
63	52690.5
64	39487.0
65	29527.0
66	22377.0
67	17410.0
68	13648.5
69	10477.0
70	8125.5
71	6247.0
72	4747.5
73	3580.0
74	2690.0
75	2054.0
76	1546.0
77	1167.5
78	909.5
79	708.5
80	539.5
81	400.5
82	312.0
83	221.0
84	172.5
85	145.5
86	118.5
87	97.0
88	65.0
89	40.5
90	29.5
91	24.0
92	16.0
93	7.0
94	5.0
95	5.5
96	7.0
97	6.0
98	3.0
99	4.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	2.378190919086067E-4
6	0.0
7	0.0
8	1.486369324428792E-5
9	0.0
10-11	7.43184662214396E-5
12-13	8.175031284358357E-5
14-15	2.229553986643188E-5
16-17	5.202292635500773E-5
18-19	7.654802020808278E-4
20-21	5.502420842099408E-4
22-23	0.0
24-25	1.1914551517292073E-4
26-27	1.4161405890712555E-4
28-29	0.0
30-31	0.0
32-33	4.49776393665889E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	5.5308030172900805E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.5281286460194413E-4
76-77	3.430731220906327E-5
78-79	4.508419299641338E-4
80-81	3.507297941715898E-5
82-83	8.8692401713076E-6
84-85	4.93271242143086E-4
86-87	1.3607329306712922E-4
88-89	8.263918711688128E-5
90-91	2.79081112413593E-5
92-93	6.603107044854907E-4
94-95	4.1174267117196137E-4
96-97	3.225624517561424E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2375.0
20-21	4590.0
22-23	4993.0
24-25	5732.0
26-27	7662.0
28-29	10519.0
30-31	17268.0
32-33	20457.0
34-35	26167.0
36-37	37646.0
38-39	42638.0
40-41	39193.0
42-43	40950.0
44-45	46530.0
46-47	50210.0
48-49	30419.0
50-51	30063.0
52-53	10667.0
54-55	10809.0
56-57	10484.0
58-59	10315.0
60-61	11585.0
62-63	54413.0
64-65	56413.0
66-67	60687.0
68-69	61773.0
70-71	60921.0
72-73	58300.0
74-75	58990.0
76-77	61889.0
78-79	64235.0
80-81	65634.0
82-83	62814.0
84-85	62659.0
86-87	65302.0
88-89	69540.0
90-91	73879.0
92-93	76456.0
94-95	91351.0
96-97	226720.0
98-99	426401.0
100-101	4498154.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.15520464494362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.78615700773493	74.71817212624028
2	7.773079185009006	13.08290139071569
3	1.987313123270091	5.017282277471296
4	0.6938475982573723	2.335635464950072
5	0.30167867129437875	1.269391515989656
6	0.1644369002993894	0.8302932597525183
7	0.08736351810754597	0.5146466321389939
8	0.05768178410514163	0.38833778765229204
9	0.0362074142801484	0.2742338122569128
>10	0.1099684044510656	1.3651706387488298
>50	0.0016937402814948604	0.09508986494594568
>100	5.448254888069022E-4	0.08905683090889432
>500	2.782742060279986E-5	0.019788398228562633
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.459107973286376E-5	0.0	0.0	0.0
3	0.0	4.459107973286376E-5	0.0	0.0	0.0
4	0.0	4.459107973286376E-5	0.0	0.0	0.0
5	0.0	4.459107973286376E-5	0.0	1.486369324428792E-5	0.0
6	1.3377323919859128E-4	4.459107973286376E-5	0.0	2.972738648857584E-5	0.0
7	1.3377323919859128E-4	4.459107973286376E-5	0.0	2.972738648857584E-5	0.0
8	1.3377323919859128E-4	8.918215946572752E-5	0.0	2.972738648857584E-5	0.0
9	1.3377323919859128E-4	1.635006256871671E-4	0.0	2.972738648857584E-5	0.0
10-11	1.3377323919859128E-4	1.635006256871671E-4	0.0	3.71592331107198E-5	0.0
12-13	1.3377323919859128E-4	1.635006256871671E-4	0.0	4.459107973286376E-5	0.0
14-15	1.3377323919859128E-4	2.5268278515289464E-4	0.0	4.459107973286376E-5	0.0
16-17	1.635006256871671E-4	2.5268278515289464E-4	0.0	4.459107973286376E-5	1.486369324428792E-5
18-19	1.635006256871671E-4	2.5268278515289464E-4	0.0	5.945477297715168E-5	1.486369324428792E-5
20-21	1.635006256871671E-4	2.5268278515289464E-4	0.0	8.918215946572753E-5	1.486369324428792E-5
22-23	1.635006256871671E-4	2.601146317750386E-4	0.0	1.6350062568716713E-4	2.229553986643188E-5
24-25	1.635006256871671E-4	2.749783250193265E-4	0.0	3.121375581300463E-4	2.972738648857584E-5
26-27	1.635006256871671E-4	2.824101716414705E-4	0.0	8.323668216801235E-4	3.71592331107198E-5
28-29	1.635006256871671E-4	2.824101716414705E-4	0.0	0.004407085046931368	4.459107973286376E-5
30-31	1.7093247230931107E-4	3.195694047521903E-4	0.0	0.016193993789651688	4.459107973286376E-5
32-33	1.7836431893145503E-4	3.270012513743342E-4	0.0	0.04998660038054027	5.945477297715168E-5
34-35	1.7836431893145503E-4	3.6416048448505405E-4	0.0	0.09341088019372744	8.918215946572752E-5
36-37	1.7836431893145503E-4	4.0131971759577384E-4	0.0	0.15280619839790197	8.918215946572752E-5
38-39	1.7836431893145503E-4	4.087515642179178E-4	0.0	0.22003468294181622	8.918215946572752E-5
40-41	1.7836431893145503E-4	4.756381838172134E-4	0.0	0.28339117539559344	8.918215946572752E-5
42-43	1.7836431893145503E-4	4.905018770615013E-4	0.0	0.39728422487994963	8.918215946572752E-5
44-45	1.7836431893145503E-4	5.202292635500773E-4	0.0	0.5014341234426751	8.918215946572752E-5
46-47	1.7836431893145503E-4	5.350929567943651E-4	0.0	0.6080439632373302	1.1890954595430335E-4
48-49	1.85796165553599E-4	5.350929567943651E-4	0.0	0.6784607099821442	1.1890954595430335E-4
50-51	2.0809170542003088E-4	6.317069628822366E-4	0.0	1.0390539080885692	1.1890954595430335E-4
52-53	2.0809170542003088E-4	6.391388095043806E-4	0.0	1.512484833459006	1.4120508582073522E-4
54-55	2.0809170542003088E-4	6.540025027486684E-4	0.0	2.135035761302761	1.635006256871671E-4
56-57	2.0809170542003088E-4	6.540025027486684E-4	0.0	2.8114824408503045	1.7093247230931107E-4
58-59	2.0809170542003088E-4	6.688661959929564E-4	0.0	3.5151148153416503	1.9322801217574296E-4
60-61	2.3038724528646277E-4	6.911617358593882E-4	0.0	4.206990008476764	1.9322801217574296E-4
62-63	2.378190919086067E-4	6.985935824815323E-4	0.0	4.7110624374703	1.9322801217574296E-4
64-65	2.378190919086067E-4	8.472305149244114E-4	0.0	4.718702375797864	1.9322801217574296E-4
66-67	2.5268278515289464E-4	8.695260547908433E-4	0.0	4.72246289018867	1.9322801217574296E-4
68-69	2.5268278515289464E-4	8.992534412794192E-4	0.0	4.725881539634855	1.9322801217574296E-4
70-71	2.5268278515289464E-4	9.066852879015631E-4	0.0	4.728943460443179	1.9322801217574296E-4
72-73	2.6754647839718256E-4	9.066852879015631E-4	0.0	4.73175269846635	2.378190919086067E-4
74-75	2.6754647839718256E-4	9.36412674390139E-4	0.0	4.734168048618546	2.378190919086067E-4
76-77	2.749783250193265E-4	9.512763676344268E-4	0.0	4.736078033200437	2.378190919086067E-4
78-79	2.824101716414705E-4	9.661400608787148E-4	0.0	4.737594129911354	2.378190919086067E-4
80-81	2.824101716414705E-4	9.661400608787148E-4	0.0	4.7384116330397905	2.378190919086067E-4
82-83	2.824101716414705E-4	9.661400608787148E-4	0.0	4.738820384604008	2.378190919086067E-4
84-85	2.824101716414705E-4	9.810037541230028E-4	0.0	4.738976453383073	2.378190919086067E-4
86-87	2.824101716414705E-4	9.958674473672907E-4	0.0	4.739006180769562	2.378190919086067E-4
88	2.824101716414705E-4	9.958674473672907E-4	0.0	4.739006180769562	2.378190919086067E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4775	0.0	33.095886	1
GTATCAA	8675	0.0	31.058563	1
TCAACGC	8510	0.0	29.681961	4
CAACGCA	8570	0.0	29.474154	5
ATCAACG	8565	0.0	29.440947	3
AACGCAG	9160	0.0	27.575706	6
TATCAAC	9885	0.0	26.470505	2
CGCAGAG	11170	0.0	22.497593	8
ACGCAGA	11195	0.0	22.447353	7
TACATGG	18745	0.0	21.491287	2
TGTAGAT	9305	0.0	21.440338	94
GTACATG	19365	0.0	21.405235	1
ACATGGG	19030	0.0	20.352602	3
GGTGTAG	10575	0.0	19.781271	94
GTGTAGA	9640	0.0	19.020891	94
CATGGGG	14365	0.0	18.485693	4
AGGTGTA	13005	0.0	16.929089	94
GCAGAGT	14915	0.0	16.646036	9
TTAGGTG	16650	0.0	15.665948	94
TAGGTGT	14070	0.0	15.555901	94
>>END_MODULE