FastQCFastQC Report
Fri 27 May 2016
SRR2140323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8701529
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69000.037.7513661
GTATCAA116250.030.7491971
TCAACGC117100.029.0776824
ATCAACG117600.028.8431193
CAACGCA117800.028.794155
AACGCAG123350.027.6396066
TATCAAC132800.026.393192
GTACATG255450.022.8795931
ACGCAGA150500.022.5381377
CGCAGAG151350.022.3828288
TACATGG255900.021.8877182
ACATGGG258900.021.0126193
CATGGGG194400.018.7904834
GCAGAGT196550.017.0806439
ATGGGGG123250.015.4540425
GTGTAGA126750.015.37026494
AGAGTAC170250.015.12148710-11
GTATAAG38250.015.1208131
CAGAGTA193800.015.0903210-11
GTACTAG24700.014.9650051
Produced by FastQC (version 0.11.5)