##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140323_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8701529
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.821195677219485	33.0	31.0	34.0	30.0	34.0
2	31.939355370763	33.0	31.0	34.0	30.0	34.0
3	31.98964113088631	34.0	31.0	34.0	30.0	34.0
4	35.50302803105063	37.0	35.0	37.0	33.0	37.0
5	35.55717977840446	37.0	35.0	37.0	33.0	37.0
6	35.60987902241089	37.0	35.0	37.0	33.0	37.0
7	35.505031816822076	37.0	35.0	37.0	33.0	37.0
8	35.479470332168056	37.0	35.0	37.0	33.0	37.0
9	37.09724199045938	39.0	37.0	39.0	33.0	39.0
10-11	37.07139164852522	39.0	37.0	39.0	33.0	39.0
12-13	37.019209325165725	39.0	37.0	39.0	33.0	39.0
14-15	38.381202372594515	40.0	38.0	41.0	33.0	41.0
16-17	38.39582790564739	40.0	38.0	41.0	33.0	41.0
18-19	38.41012797865754	40.0	38.0	41.0	33.0	41.0
20-21	38.46369361889355	40.0	38.0	41.0	33.0	41.0
22-23	38.445167499477556	40.0	38.0	41.0	33.0	41.0
24-25	38.45185228208515	40.0	38.0	41.0	33.0	41.0
26-27	38.425589525470954	40.0	38.0	41.0	33.0	41.0
28-29	38.39830328128363	40.0	38.0	41.0	33.0	41.0
30-31	38.34858951200367	40.0	38.0	41.0	33.0	41.0
32-33	38.30531824656922	40.0	38.0	41.0	33.0	41.0
34-35	38.25096605030935	40.0	38.0	41.0	33.0	41.0
36-37	38.191996197078026	40.0	38.0	41.0	33.0	41.0
38-39	38.11032090581545	40.0	38.0	41.0	33.0	41.0
40-41	38.04150910461047	40.0	38.0	41.0	32.5	41.0
42-43	37.96113025395843	40.0	37.0	41.0	32.0	41.0
44-45	37.84580065136524	40.0	37.0	41.0	32.0	41.0
46-47	37.74331403490143	40.0	37.0	41.0	32.0	41.0
48-49	37.64356538519599	40.0	37.0	41.0	32.0	41.0
50-51	37.51278947425328	40.0	36.0	41.0	31.0	41.0
52-53	37.392000774778836	40.0	36.0	41.0	31.0	41.0
54-55	37.25468620360657	39.0	36.0	41.0	31.0	41.0
56-57	37.07854976496399	39.0	35.5	41.0	31.0	41.0
58-59	37.23434284019656	39.0	35.5	41.0	31.0	41.0
60-61	37.29863333661136	39.0	35.5	41.0	31.0	41.0
62-63	37.14381670900703	39.0	35.0	41.0	31.0	41.0
64-65	36.94111535234326	39.0	35.0	40.5	31.0	41.0
66-67	36.67914910668388	38.0	35.0	40.0	31.0	41.0
68-69	36.40416585414599	37.5	35.0	40.0	31.0	41.0
70-71	36.169899786659485	37.0	35.0	40.0	31.0	41.0
72-73	35.92770188683121	36.5	34.5	39.0	31.0	41.0
74-75	35.65344403321973	36.0	34.0	39.0	30.0	41.0
76-77	35.40066632525681	36.0	34.0	39.0	30.0	40.5
78-79	35.07386091729278	35.0	34.0	37.5	30.0	39.5
80-81	34.77748008087828	35.0	34.0	37.0	30.0	39.0
82-83	34.493046779690175	35.0	34.0	36.5	30.0	39.0
84-85	34.26384365843405	35.0	34.0	36.0	30.0	37.5
86-87	34.03637030077225	35.0	34.0	36.0	30.0	37.0
88-89	33.876254269494034	35.0	34.0	35.5	30.0	37.0
90-91	33.74935549098092	35.0	34.0	35.0	30.0	36.0
92-93	33.652788300535164	35.0	34.0	35.0	30.0	36.0
94-95	33.612229848655076	35.0	34.0	35.0	30.0	36.0
96-97	33.61201214873296	35.0	34.0	35.0	30.0	36.0
98-99	33.65525626921958	35.0	34.0	35.0	30.0	36.0
100	33.309563316057876	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	24.0
17	256.0
18	1117.0
19	3670.0
20	9223.0
21	18733.0
22	31151.0
23	43817.0
24	57827.0
25	72466.0
26	84075.0
27	92639.0
28	105555.0
29	128805.0
30	152951.0
31	178804.0
32	223833.0
33	291809.0
34	394727.0
35	551195.0
36	849770.0
37	1555983.0
38	2358699.0
39	1418001.0
40	76398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.024537181913665	20.588301205454812	14.309898869497534	24.077262743133996
2	13.95235251184016	20.99774648800228	42.32217119543014	22.727729804727424
3	17.789930942021797	26.333360493311	30.3984736475624	25.478234917104796
4	12.085450729406292	18.17905795636606	40.01463420968889	29.720857104538755
5	13.174427561932072	38.13555786703203	35.890073779157156	12.799940791878742
6	29.231851091917292	38.2800769841714	19.674059581942437	12.814012341968867
7	26.243930950977806	32.72088534335579	23.6759796670194	17.359204038647004
8	23.921818797592927	37.10553627988828	21.717999215999857	17.254645706518936
9	25.180011466950237	17.612410416606092	21.729732786042543	35.47784533040113
10-11	23.747956608298164	27.15328478897177	29.440745763487104	19.658012839242957
12-13	24.414867731948533	24.67528213907106	29.332919075993143	21.576931052987263
14-15	21.721239766663878	26.221623578718877	26.658276949056454	25.39885970556079
16-17	21.60387339401548	29.315345739753855	27.327801270491257	21.752979595739408
18-19	21.64020270173207	29.022473827652963	28.37400756540142	20.963315905213552
20-21	22.58752703591616	28.056523073388796	28.055798704575608	21.300151186119436
22-23	22.324567360625842	27.972081476563528	27.843409867254877	21.85994129555576
24-25	22.060890673957797	28.119786242302975	27.9266946063584	21.89262847738083
26-27	22.006065066017168	28.276117454304007	27.788764186333687	21.929053293345135
28-29	21.930264297002715	28.321478034256614	27.833982322058183	21.91427534668249
30-31	22.19894303759333	28.16721452376178	27.771366325612846	21.86247611303204
32-33	21.991978253677306	28.204820549080296	27.78175029559468	22.021450901647714
34-35	22.137808956680153	28.16989981649024	27.889394110343112	21.8028971164865
36-37	22.079935350214743	27.978803600856217	27.935465627548105	22.00579542138093
38-39	21.966339897996576	28.064741607526116	28.05753794280629	21.911380551671016
40-41	22.12230359425271	27.867422818353816	28.062973216111896	21.947300371281578
42-43	21.8369454868556	27.92636285807044	28.048087806470352	22.188603848603602
44-45	21.917932056428814	27.972816888150575	28.08731322562942	22.021937829791188
46-47	21.746271522968293	27.92353590597167	28.02788933084155	22.30230324021849
48-49	21.684993381478606	27.95924498693526	28.076320927958232	22.279440703627902
50-51	21.56722816580706	27.965607550704707	28.147203303824526	22.31996097966371
52-53	21.68226076258238	27.90962648422611	28.069946145585007	22.338166607606507
54-55	21.751463353555884	27.66596793536299	28.138399233050528	22.444169478030602
56-57	21.686151689848334	27.53099831306561	28.170217169025303	22.61263282806076
58-59	21.578665171325497	27.51259645906367	28.30814344958337	22.60059492002747
60-61	21.64367110818625	27.34800690973304	28.519648306942734	22.48867367513797
62-63	21.556835002947096	27.534099049241505	28.447936484304737	22.461129463506666
64-65	21.495481649009022	27.730748782121985	28.150535674316117	22.623233894552875
66-67	21.264244551707236	27.949077111626703	27.960176903476096	22.826501433189968
68-69	21.35068748599598	28.056131746800812	27.99731800295223	22.595862764250978
70-71	21.370104735486674	28.140795730158562	27.67098745722672	22.818112077128042
72-73	21.608034925418345	27.972751741489017	27.37113480595404	23.048078527138603
74-75	21.809653708696054	27.86007122452489	27.49662733513265	22.833647731646405
76-77	21.941159227467587	27.500848543042444	27.595502277337857	22.96248995215211
78-79	22.17127674849564	27.23533517386956	27.562335361503465	23.031052716131338
80-81	22.537081773925344	27.24266311249654	27.238230220950182	22.982024892627933
82-83	22.27028213318366	27.3063749921838	27.47599155837136	22.947351316261177
84-85	22.41994537130938	27.497640478166208	27.346739588247214	22.735674562277193
86-87	22.1472004139211	27.801967475102842	27.499027437093744	22.551804673882316
88-89	21.924013772583077	27.883442355701437	27.483533777887825	22.70901009382766
90-91	21.778221277637513	28.032912684533446	27.75674334935135	22.432122688477698
92-93	21.771594743380316	27.667120012394246	28.22611877725918	22.33516646696626
94-95	21.418525832680903	27.67184376896558	28.593832532393932	22.31579786595959
96-97	21.770424216434307	27.590308384375888	28.507693626482805	22.131573772706993
98-99	23.142092716177093	29.529523792765733	28.79732547686544	18.531058014191736
100	22.27946111637755	30.768313947454	22.51655468755406	24.43567024861439
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2362.0
1	1778.0
2	1329.5
3	1458.5
4	1446.0
5	1527.0
6	1772.5
7	2082.0
8	2331.0
9	2640.0
10	2972.5
11	3253.0
12	3620.0
13	4128.5
14	4822.5
15	5731.5
16	6836.5
17	8237.0
18	10014.0
19	12346.5
20	15341.0
21	19328.5
22	24762.5
23	32159.5
24	41920.0
25	54014.5
26	69433.5
27	87790.0
28	107221.0
29	129614.5
30	151698.0
31	171987.5
32	193986.0
33	213816.5
34	232598.5
35	251347.0
36	272246.5
37	293580.5
38	307098.0
39	315478.0
40	328200.0
41	341230.5
42	348783.5
43	355713.0
44	362432.0
45	368783.0
46	371349.0
47	369747.5
48	365850.0
49	358679.0
50	349624.5
51	337621.0
52	321220.0
53	301498.0
54	281329.5
55	258357.5
56	234323.0
57	209632.5
58	184725.5
59	160476.0
60	133328.5
61	107376.5
62	84704.0
63	65292.0
64	48638.0
65	36126.5
66	27467.5
67	21313.5
68	16714.0
69	12719.0
70	9871.5
71	7535.5
72	5626.0
73	4217.0
74	3133.0
75	2371.0
76	1829.0
77	1425.0
78	1105.5
79	856.5
80	653.5
81	488.5
82	379.5
83	270.5
84	195.5
85	153.5
86	129.5
87	104.5
88	68.0
89	38.5
90	24.0
91	19.5
92	14.5
93	7.5
94	4.5
95	3.5
96	5.0
97	6.5
98	3.5
99	2.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	2.2984466293222717E-4
6	0.0
7	1.149223314661136E-5
8	0.0
9	0.0
10-11	4.022281601313976E-5
12-13	1.5514514747925337E-4
14-15	5.171504915975112E-5
16-17	1.4939903090594768E-4
18-19	8.504252528492407E-4
20-21	5.116547183792067E-4
22-23	0.0
24-25	1.2089441139677373E-4
26-27	2.131849105415617E-4
28-29	0.0
30-31	0.0
32-33	7.524433136700602E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	3.0093414170663726E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.8755855545763705E-4
76-77	1.3074621440679463E-5
78-79	4.23282298936113E-4
80-81	3.3481042765737695E-5
82-83	4.747362653542425E-5
84-85	4.532568876676286E-4
86-87	7.653860690829117E-5
88-89	2.821671623168012E-5
90-91	3.579176209645236E-5
92-93	5.74342868151961E-4
94-95	4.2895397945044196E-4
96-97	3.782924017171751E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2898.0
20-21	5588.0
22-23	6163.0
24-25	6909.0
26-27	8839.0
28-29	11708.0
30-31	16752.0
32-33	18085.0
34-35	22027.0
36-37	33914.0
38-39	41004.0
40-41	34425.0
42-43	36629.0
44-45	43752.0
46-47	55945.0
48-49	35347.0
50-51	35367.0
52-53	14509.0
54-55	14538.0
56-57	14632.0
58-59	14765.0
60-61	16065.0
62-63	69116.0
64-65	73506.0
66-67	78077.0
68-69	80056.0
70-71	80465.0
72-73	79641.0
74-75	81166.0
76-77	86680.0
78-79	92317.0
80-81	94771.0
82-83	92243.0
84-85	93700.0
86-87	96879.0
88-89	101301.0
90-91	106469.0
92-93	114321.0
94-95	131485.0
96-97	305419.0
98-99	573216.0
100-101	5780840.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.61992763723289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.79309465079493	71.6566603244662
2	8.118081570127403	13.251944606139052
3	2.1641984197355284	5.299251552342828
4	0.8522825586571356	2.7825296305628444
5	0.40170141056607006	1.6393420031088508
6	0.20953801932872737	1.02614867849159
7	0.12675167557689285	0.724182381193881
8	0.08599566256073297	0.5615167804258339
9	0.055288531049548104	0.40613813130897175
>10	0.18900170497145752	2.3458623735685378
>50	0.00323578730808188	0.1738088738776906
>100	8.04667446139822E-4	0.10998634445885092
>500	0.0	0.0
>1k	2.5341877390517682E-5	0.022628320054934155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.298446629322272E-5	0.0	0.0	0.0
3	0.0	2.298446629322272E-5	0.0	1.149223314661136E-5	0.0
4	0.0	9.193786517289087E-5	0.0	6.895339887966816E-5	0.0
5	2.298446629322272E-5	9.193786517289087E-5	0.0	1.0343009831950224E-4	0.0
6	3.447669943983408E-5	9.193786517289087E-5	0.0	1.0343009831950224E-4	1.149223314661136E-5
7	4.596893258644544E-5	9.193786517289087E-5	0.0	1.149223314661136E-4	1.149223314661136E-5
8	4.596893258644544E-5	9.193786517289087E-5	0.0	1.2641456461272497E-4	1.149223314661136E-5
9	4.596893258644544E-5	2.87305828665284E-4	0.0	1.3790679775933632E-4	2.298446629322272E-5
10-11	8.044563202627952E-5	2.87305828665284E-4	0.0	2.0686019663900449E-4	2.298446629322272E-5
12-13	1.0343009831950224E-4	2.9305194523858967E-4	0.0	2.4708301265214424E-4	2.298446629322272E-5
14-15	1.1492233146611361E-4	4.941660253042885E-4	0.0	2.9305194523858967E-4	3.447669943983408E-5
16-17	1.4939903090594768E-4	5.171504915975111E-4	0.0	3.6200534411825786E-4	5.1715049159751115E-5
18-19	1.8387573034578175E-4	5.573733076106509E-4	0.0	4.0222816013139764E-4	6.895339887966816E-5
20-21	2.0686019663900449E-4	6.090883567704021E-4	0.0	5.114043750242055E-4	6.895339887966816E-5
22-23	2.2409854635892153E-4	7.067723385165987E-4	0.0	8.102024368361008E-4	7.469951545297384E-5
24-25	2.5857524579875563E-4	7.412490379564327E-4	0.0	0.0015227208919260053	8.044563202627952E-5
26-27	2.9879806181189536E-4	7.584873876763498E-4	0.0	0.003436177710836797	8.044563202627952E-5
28-29	3.1029029495850674E-4	7.699796208229611E-4	0.0	0.014089477837745529	8.044563202627952E-5
30-31	3.505131109716465E-4	9.653475843153542E-4	0.0	0.051146183618993854	8.61917485995852E-5
32-33	4.0222816013139764E-4	9.653475843153542E-4	0.0	0.15480612660142834	9.193786517289087E-5
34-35	4.367048595712317E-4	0.0010687776826348565	0.0	0.29470108069512846	1.0343009831950224E-4
36-37	4.539432092911487E-4	0.0012066844803941927	0.0	0.49172392576063356	1.0343009831950224E-4
38-39	4.596893258644544E-4	0.0012813839958471665	0.0	0.7158167260029817	1.0343009831950224E-4
40-41	4.596893258644544E-4	0.001419290793606503	0.0	0.9329050101424703	1.0343009831950224E-4
42-43	4.596893258644544E-4	0.0014997364256327823	0.0	1.2527740814286776	1.0343009831950224E-4
44-45	4.7692767558437147E-4	0.0016491354565387301	0.0	1.5268868264416517	1.0343009831950224E-4
46-47	4.884199087309827E-4	0.0017698039045781495	0.0	1.794948910702935	1.0917621489280792E-4
48-49	4.941660253042885E-4	0.0017812961377247608	0.0	1.9550816873678178	1.3790679775933632E-4
50-51	4.941660253042885E-4	0.0019192029354840971	0.0	2.3521613270495334	1.3790679775933632E-4
52-53	4.999121418775942E-4	0.0019192029354840971	0.0	2.833772087641149	1.4939903090594768E-4
54-55	5.056582584508999E-4	0.0019306951686307084	0.0	3.4388266705770905	1.6663738062586473E-4
56-57	5.286427247441225E-4	0.001953679634923931	0.0	4.087936729280567	1.723834971991704E-4
58-59	5.286427247441225E-4	0.001970917984643848	0.0	4.759755440681747	1.7812961377247606E-4
60-61	5.343888413174283E-4	0.002022633033803599	0.0	5.424529413164054	1.8387573034578175E-4
62-63	5.458810744640395E-4	0.0020743480829633508	0.0	5.913409011220901	1.8387573034578175E-4
64-65	5.57373307610651E-4	0.002298446629322272	0.0	5.922959056965736	1.8387573034578175E-4
66-67	5.631194241839567E-4	0.002355907795055329	0.0	5.926475680308599	1.8387573034578175E-4
68-69	5.975961236237907E-4	0.002401876727641774	0.0	5.929727982289089	1.8387573034578175E-4
70-71	6.033422401970964E-4	0.0024133689607883854	0.0	5.932336719213371	1.8387573034578175E-4
72-73	6.148344733437078E-4	0.0024133689607883854	0.0	5.9350373940028245	1.9536796349239313E-4
74-75	6.205805899170135E-4	0.002459337893374831	0.0	5.937536954712212	1.9536796349239313E-4
76-77	6.205805899170135E-4	0.002585752457987556	0.0	5.939795178525522	2.0111408006569882E-4
78-79	6.320728230636248E-4	0.0026661980900138356	0.0	5.9415879668963925	2.0686019663900449E-4
80-81	6.378189396369304E-4	0.0026719442065871414	0.0	5.942363692633789	2.2984466293222722E-4
82-83	6.493111727835419E-4	0.002689182556307058	0.0	5.942846366425947	2.5282912922544994E-4
84-85	6.550572893568475E-4	0.002706420906026975	0.0	5.943024496039719	2.5282912922544994E-4
86-87	6.550572893568475E-4	0.0027351514888935037	0.0	5.943087703322025	2.5282912922544994E-4
88	6.550572893568475E-4	0.002942011685532508	0.0	5.943104941671745	2.5282912922544994E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6900	0.0	37.751366	1
GTATCAA	11625	0.0	30.749197	1
TCAACGC	11710	0.0	29.077682	4
ATCAACG	11760	0.0	28.843119	3
CAACGCA	11780	0.0	28.79415	5
AACGCAG	12335	0.0	27.639606	6
TATCAAC	13280	0.0	26.39319	2
GTACATG	25545	0.0	22.879593	1
ACGCAGA	15050	0.0	22.538137	7
CGCAGAG	15135	0.0	22.382828	8
TACATGG	25590	0.0	21.887718	2
ACATGGG	25890	0.0	21.012619	3
CATGGGG	19440	0.0	18.790483	4
GCAGAGT	19655	0.0	17.080643	9
ATGGGGG	12325	0.0	15.454042	5
GTGTAGA	12675	0.0	15.370264	94
AGAGTAC	17025	0.0	15.121487	10-11
GTATAAG	3825	0.0	15.120813	1
CAGAGTA	19380	0.0	15.09032	10-11
GTACTAG	2470	0.0	14.965005	1
>>END_MODULE