FastQCFastQC Report
Fri 27 May 2016
SRR2140322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7521383
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.038.496141
GTATCAA102700.032.0330051
TCAACGC108650.028.857614
ATCAACG109700.028.58143
CAACGCA111550.028.1073935
AACGCAG117800.026.7615876
TATCAAC123800.025.9497932
ACGCAGA140600.022.2998477
CGCAGAG143250.021.9172638
GTACATG238900.021.5804771
TACATGG237300.021.491012
ACATGGG242300.020.3571593
CATGGGG182450.018.1252174
GCAGAGT179600.017.433579
AGAGTAC151700.016.04538310-11
CAGAGTA173650.015.28923310-11
GTATAAG34450.014.5669751
ATGGGGG115800.014.4083345
GTGGTAT42750.014.247051
GAGTACA176300.014.2080111
Produced by FastQC (version 0.11.5)