FastQCFastQC Report
Fri 27 May 2016
SRR2140321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8967489
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77050.041.5140881
GTATCAA130800.033.3685721
ATCAACG140650.029.373763
TCAACGC140600.029.3529154
CAACGCA142650.028.838725
AACGCAG149450.027.5854346
TATCAAC156900.027.0325982
ACGCAGA178650.023.0520177
CGCAGAG182750.022.4866968
GTACATG308200.022.0133171
TCGGTGG38900.021.78535794
TACATGG306950.021.4865992
ACATGGG309050.020.7996083
CATGGGG230900.018.045124
GCAGAGT228700.017.6608929
GGACCGA14200.016.421916
AGAGTAC199350.016.14485710-11
CAGAGTA224250.015.92180210-11
ATGGGGG139350.015.4080975
GTACTAG24300.015.3902751
Produced by FastQC (version 0.11.5)