##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8967489
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.735882140474327	33.0	31.0	34.0	30.0	34.0
2	31.841158879592715	33.0	31.0	34.0	30.0	34.0
3	31.89414807199652	34.0	31.0	34.0	30.0	34.0
4	35.399351479550184	37.0	35.0	37.0	33.0	37.0
5	35.46334620538704	37.0	35.0	37.0	33.0	37.0
6	35.50857748473402	37.0	35.0	37.0	33.0	37.0
7	35.39153524470451	37.0	35.0	37.0	32.0	37.0
8	35.3664431592835	37.0	35.0	37.0	32.0	37.0
9	36.944856692882475	39.0	37.0	39.0	33.0	39.0
10-11	36.92204094144972	39.0	37.0	39.0	33.0	39.0
12-13	36.86545854698009	39.0	37.0	39.0	33.0	39.0
14-15	38.20385455727907	40.0	38.0	41.0	33.0	41.0
16-17	38.222781761984876	40.0	38.0	41.0	33.0	41.0
18-19	38.24796272401338	40.0	38.0	41.0	33.0	41.0
20-21	38.30830394231312	40.0	38.0	41.0	33.0	41.0
22-23	38.293718134061564	40.0	38.0	41.0	33.0	41.0
24-25	38.30762176825931	40.0	38.0	41.0	33.0	41.0
26-27	38.285458673907385	40.0	38.0	41.0	33.0	41.0
28-29	38.258760062618485	40.0	38.0	41.0	33.0	41.0
30-31	38.20841208697204	40.0	38.0	41.0	33.0	41.0
32-33	38.16450590275021	40.0	38.0	41.0	33.0	41.0
34-35	38.10890157313321	40.0	38.0	41.0	32.5	41.0
36-37	38.05175155817243	40.0	38.0	41.0	32.0	41.0
38-39	37.974601429341185	40.0	37.5	41.0	32.0	41.0
40-41	37.910299586897466	40.0	37.0	41.0	32.0	41.0
42-43	37.830376825852724	40.0	37.0	41.0	32.0	41.0
44-45	37.725546353788424	40.0	37.0	41.0	32.0	41.0
46-47	37.63356394412472	40.0	37.0	41.0	31.0	41.0
48-49	37.53777946299398	40.0	36.5	41.0	31.0	41.0
50-51	37.4071134577797	40.0	36.0	41.0	31.0	41.0
52-53	37.28996983241721	39.5	36.0	41.0	31.0	41.0
54-55	37.15506782593627	39.0	36.0	41.0	31.0	41.0
56-57	36.984561354129326	39.0	35.0	41.0	30.0	41.0
58-59	37.142654524338326	39.0	35.0	41.0	31.0	41.0
60-61	37.21158220104937	39.0	35.0	41.0	31.0	41.0
62-63	37.059749284705234	39.0	35.0	41.0	31.0	41.0
64-65	36.860925998366355	38.5	35.0	40.5	31.0	41.0
66-67	36.59672340703099	38.0	35.0	40.0	31.0	41.0
68-69	36.32218795396699	37.5	35.0	40.0	30.5	41.0
70-71	36.0822043686524	37.0	35.0	40.0	30.0	41.0
72-73	35.83503759723166	36.5	34.0	39.0	30.0	41.0
74-75	35.55461131428328	36.0	34.0	39.0	30.0	41.0
76-77	35.28420312324616	36.0	34.0	39.0	30.0	40.5
78-79	34.95377200495659	35.0	34.0	37.5	30.0	39.5
80-81	34.63218707269439	35.0	34.0	37.0	30.0	39.0
82-83	34.34230022402616	35.0	34.0	36.5	30.0	39.0
84-85	34.09894806423988	35.0	34.0	36.0	29.0	37.5
86-87	33.88649496109173	35.0	34.0	36.0	29.0	37.0
88-89	33.72745265838965	35.0	34.0	35.5	29.0	37.0
90-91	33.58922417605851	35.0	33.0	35.0	29.0	36.0
92-93	33.47904604939526	35.0	33.0	35.0	29.0	36.0
94-95	33.44901976224649	35.0	33.0	35.0	29.0	36.0
96-97	33.46242445390648	35.0	33.0	35.0	29.0	36.0
98-99	33.56705496473188	35.0	33.5	35.0	30.0	36.0
100	33.21196230856463	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	63.0
17	400.0
18	1332.0
19	4153.0
20	10774.0
21	21942.0
22	35581.0
23	50923.0
24	67482.0
25	83709.0
26	96214.0
27	104640.0
28	118779.0
29	143951.0
30	169592.0
31	196178.0
32	244886.0
33	316529.0
34	426761.0
35	592005.0
36	907189.0
37	1629638.0
38	2348103.0
39	1321615.0
40	75050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.87325894684677	20.805054792930328	14.491960904551988	23.829725355670913
2	13.84463365385784	20.95107114154252	42.58680439976006	22.617490804839573
3	17.664119799868168	26.58382965398675	30.400539102975205	25.351511443169876
4	11.97530323148431	18.195729038530185	40.125056189084816	29.70391154090069
5	12.954370174501879	38.47095969564653	35.90439352557489	12.670276604276703
6	29.09953945859315	38.39246415579657	19.689770458597717	12.818225927012566
7	26.1741162994457	32.88893914450299	23.679125784263576	17.257818771787733
8	23.919053353520333	37.20414649053854	21.65820814683088	17.218592009110246
9	25.15387529329559	17.71382156142037	21.80919318663229	35.32310995865175
10-11	23.73203404634467	27.335698731500173	29.3592990005835	19.572968221571664
12-13	24.361631968683273	24.867236890291093	29.27519404568242	21.495937095343216
14-15	21.655214458413617	26.432919956538537	26.702215097221522	25.209650487826323
16-17	21.47628711377164	29.42855216849467	27.368675011303345	21.726485706430342
18-19	21.48648511515422	29.128553186321714	28.440879634239945	20.94408206428412
20-21	22.57651836615401	28.190644337676886	28.111633270827767	21.121204025341335
22-23	22.266348603886282	28.096239727733973	27.87641174134683	21.76099992703292
24-25	21.901446329833917	28.280018860288987	28.017049799577304	21.8014850102998
26-27	21.868761605088732	28.428248696446868	27.87250744518014	21.830482253284263
28-29	21.782075890801405	28.53203117192567	27.92806461464646	21.757828322626462
30-31	22.046737272685935	28.306260117168097	27.87980488916397	21.767197720981997
32-33	21.856781195602256	28.383741137298713	27.889836717058557	21.869640950040473
34-35	21.987759748140743	28.334295741113525	27.99460189085705	21.68334261988868
36-37	21.938276052852647	28.146450870784452	28.01008290973629	21.90519016662661
38-39	21.79295422763974	28.180082890375736	28.180308946816606	21.846653935167918
40-41	21.974068782280128	27.94107128789523	28.214722316523627	21.870137613301015
42-43	21.69463260967363	27.89658544641887	28.250199959573607	22.158581984333892
44-45	21.701484040379974	27.953278020844053	28.329424340579006	22.015813598196964
46-47	21.569658425249465	27.85301945340152	28.289918185435937	22.287403935913083
48-49	21.439113434512773	27.77937094790882	28.498855715546384	22.28265990203203
50-51	21.26534978485001	27.861444516634343	28.480055441232103	22.39315025728354
52-53	21.2468187259223	27.843144604388375	28.50532759887296	22.404709070816367
54-55	21.265451667619466	27.697089986049946	28.58083474473095	22.45662360159964
56-57	21.25400704167185	27.771660342291604	28.473825876107895	22.500506739928642
58-59	21.216045899492066	27.831424513429692	28.471587871221022	22.48094171585722
60-61	21.2911469026706	27.633502502146385	28.422744660999587	22.652605934183427
62-63	21.220120270050845	27.54671459559561	28.419027349789268	22.814137784564277
64-65	21.31254618231533	27.646093394803934	28.30688387002633	22.734476552854414
66-67	21.185947302531464	27.781763968086047	28.004231599845152	23.028057129537338
68-69	21.29605855458072	27.88052210653298	27.83555181809088	22.98786752079542
70-71	21.39096428329179	27.81458375527083	27.73024323451145	23.064208726925926
72-73	21.555332135070326	27.803104779993358	27.31317451521666	23.328388569719653
74-75	21.593407938579205	27.744956066797076	27.170458134313925	23.491177860309797
76-77	21.839152213815986	27.52504279492366	27.29600921405579	23.339795777204568
78-79	21.949314212258823	27.313598162594026	27.41460165593146	23.322485969215688
80-81	22.07951131217244	27.23822941960522	27.41191754800421	23.27034172021813
82-83	22.04613896570691	27.343143061922902	27.404630322306218	23.20608765006397
84-85	22.234950092751788	27.16309139278262	27.43110178911105	23.17085672535454
86-87	21.859584179614387	27.42346282454572	27.71473829298453	23.002214702855362
88-89	22.02566597156561	27.518214205604668	27.489692881957673	22.96642694087205
90-91	22.000644366825668	27.80541104380876	27.69873299087188	22.495211598493693
92-93	21.742451536213224	27.80775693020892	27.99875848640979	22.451033047168064
94-95	21.49935929815233	27.836504940404982	28.292606672869862	22.37152908857282
96-97	21.60144561381777	27.77945578815808	28.525117561891868	22.093981036132277
98-99	22.973018704952235	29.83006740184257	28.626118553993113	18.570795339212083
100	22.411593390433197	30.962824057976274	22.3845786761525	24.241003875438032
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2957.0
1	2219.0
2	1610.5
3	1738.5
4	1734.0
5	1876.5
6	2175.0
7	2553.5
8	2903.5
9	3281.0
10	3736.0
11	4084.0
12	4487.5
13	5027.0
14	5807.5
15	6900.0
16	8237.0
17	9777.5
18	11639.0
19	14000.5
20	17152.5
21	21217.0
22	26664.0
23	34362.5
24	44044.5
25	56293.5
26	71901.0
27	89660.0
28	108344.0
29	130915.0
30	153726.5
31	173617.5
32	194967.0
33	215640.0
34	235440.5
35	252532.5
36	270232.5
37	290624.5
38	305689.5
39	315440.0
40	327956.0
41	339615.5
42	346060.5
43	353821.5
44	360934.0
45	367979.5
46	371769.0
47	371151.5
48	368792.0
49	361648.0
50	353999.0
51	344935.5
52	329485.5
53	308581.0
54	285302.0
55	261155.0
56	235872.0
57	208732.0
58	183244.5
59	159178.0
60	130939.0
61	104182.0
62	81792.5
63	62483.5
64	47639.0
65	36694.5
66	28391.0
67	21888.0
68	17229.5
69	13413.5
70	10503.0
71	8176.0
72	6211.5
73	4768.0
74	3605.0
75	2720.0
76	2099.5
77	1626.5
78	1227.5
79	917.5
80	690.0
81	520.0
82	406.0
83	283.0
84	208.5
85	183.0
86	168.0
87	138.5
88	85.5
89	45.0
90	26.5
91	20.5
92	19.5
93	14.5
94	13.0
95	13.5
96	10.0
97	8.0
98	5.0
99	8.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	2.3417926690515035E-4
6	0.0
7	0.0
8	2.2302787324300035E-5
9	0.0
10-11	7.248405880397511E-5
12-13	1.4496811760795022E-4
14-15	5.0181271479675084E-5
16-17	1.393924207768752E-4
18-19	8.921114929720013E-4
20-21	4.072431258197163E-4
22-23	0.0
24-25	5.028513908757727E-5
26-27	1.7336126351057452E-4
28-29	0.0
30-31	0.0
32-33	2.8078058575210364E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	6.86493239786425E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.1629003690005282E-4
76-77	1.8560755694981203E-5
78-79	3.3177362171847715E-4
80-81	5.072109225843335E-5
82-83	3.213171019356656E-5
84-85	4.16830956680452E-4
86-87	9.907993055421614E-5
88-89	4.024644238218693E-5
90-91	1.3646055437100214E-5
92-93	5.490317685765768E-4
94-95	3.9700316489505187E-4
96-97	3.053121478687794E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3206.0
20-21	6434.0
22-23	7054.0
24-25	7632.0
26-27	9618.0
28-29	12079.0
30-31	14299.0
32-33	14668.0
34-35	16860.0
36-37	22116.0
38-39	24543.0
40-41	21656.0
42-43	15289.0
44-45	15708.0
46-47	16192.0
48-49	16075.0
50-51	15997.0
52-53	15617.0
54-55	15598.0
56-57	15705.0
58-59	15285.0
60-61	17093.0
62-63	46473.0
64-65	76798.0
66-67	82135.0
68-69	84977.0
70-71	85177.0
72-73	83158.0
74-75	85699.0
76-77	92314.0
78-79	99523.0
80-81	105729.0
82-83	103639.0
84-85	105946.0
86-87	113125.0
88-89	123235.0
90-91	131962.0
92-93	139522.0
94-95	155110.0
96-97	328826.0
98-99	597580.0
100-101	6007837.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.03561571420498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.40623678539474	69.15576362666455
2	8.937085761900718	14.305703232887575
3	2.4672353527264703	5.924001017019502
4	0.9772836647044447	3.1286999932821953
5	0.46984763352215764	1.880227232040401
6	0.2556581258280633	1.2277053307793242
7	0.14543932754875605	0.8148228290597236
8	0.0889496969134958	0.5695315008050844
9	0.061863967843369054	0.44561886811810375
>10	0.18678376337293984	2.1882265386672963
>50	0.0027790749810250857	0.14600208431876874
>100	7.114476999223254E-4	0.11765087205415652
>500	5.01594623492186E-5	0.02179897075895575
>1k	7.523810153867977E-5	0.07424790354436422
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2302787324300035E-5	0.0
2	0.0	2.2302787324300035E-5	0.0	2.2302787324300035E-5	0.0
3	0.0	2.2302787324300035E-5	0.0	2.2302787324300035E-5	0.0
4	0.0	1.226653302836502E-4	0.0	2.2302787324300035E-5	0.0
5	0.0	1.226653302836502E-4	0.0	6.69083619729001E-5	0.0
6	0.0	1.226653302836502E-4	0.0	6.69083619729001E-5	1.1151393662150018E-5
7	0.0	1.226653302836502E-4	0.0	6.69083619729001E-5	1.1151393662150018E-5
8	0.0	1.5611951127010026E-4	0.0	8.921114929720014E-5	1.1151393662150018E-5
9	0.0	2.3417926690515037E-4	0.0	8.921114929720014E-5	2.2302787324300035E-5
10-11	0.0	2.3417926690515037E-4	0.0	1.6727090493225024E-4	3.345418098645005E-5
12-13	0.0	2.3417926690515037E-4	0.0	2.3417926690515037E-4	3.345418098645005E-5
14-15	0.0	4.014501718374006E-4	0.0	2.732091447226754E-4	5.0181271479675084E-5
16-17	0.0	4.1260156549955063E-4	0.0	2.8993623521590044E-4	8.363545246612514E-5
18-19	0.0	4.293286559927757E-4	0.0	3.122390225402005E-4	8.921114929720014E-5
20-21	0.0	4.460557464860007E-4	0.0	4.181772623306257E-4	9.478684612827515E-5
22-23	0.0	4.8508562430352577E-4	0.0	8.475059183234014E-4	1.1151393662150018E-4
24-25	0.0	5.129641084589008E-4	0.0	0.0017897986827750778	1.4496811760795022E-4
26-27	0.0	5.241155021210508E-4	0.0	0.004382497709224957	1.4496811760795022E-4
28-29	0.0	5.241155021210508E-4	0.0	0.017340417144643277	1.4496811760795022E-4
30-31	0.0	6.13326651418251E-4	0.0	0.06340124866615393	1.4496811760795022E-4
32-33	0.0	6.13326651418251E-4	0.0	0.1818123222676939	1.4496811760795022E-4
34-35	0.0	7.136891943776011E-4	0.0	0.34719306597420974	1.6727090493225027E-4
36-37	0.0	8.196274341680263E-4	0.0	0.6011883594170007	1.7842229859440028E-4
38-39	0.0	8.642330088166264E-4	0.0	0.9488386325313585	1.951493890876253E-4
40-41	0.0	9.590198549449015E-4	0.0	1.321964264466898	2.007250859187003E-4
42-43	0.0	9.868983391002764E-4	0.0	1.7608050592534878	2.007250859187003E-4
44-45	0.0	0.0010872608820596268	0.0	2.2936521026120023	2.007250859187003E-4
46-47	0.0	0.0011597449408636018	0.0	2.8915284981113443	2.2302787324300036E-4
48-49	0.0	0.0011708963345257518	0.0	3.4964804528893207	2.564820542294504E-4
50-51	0.0	0.001332591542626927	0.0	4.104214680386003	2.564820542294504E-4
52-53	0.0	0.0013493186331201522	0.0	4.691341132395033	2.787848415537504E-4
54-55	0.0	0.001354894329951227	0.0	5.284701213461204	3.010876288780505E-4
56-57	0.0	0.0013716214204444522	0.0	5.929162555984178	3.010876288780505E-4
58-59	0.0	0.0013939242077687522	0.0	6.599238649749111	3.010876288780505E-4
60-61	0.0	0.0014496811760795023	0.0	7.27095957407921	3.010876288780505E-4
62-63	0.0	0.0015165895380524024	0.0	7.918286824773356	3.2339041620235053E-4
64-65	0.0	0.0016838604429846526	0.0	8.082992909163313	3.2339041620235053E-4
66-67	0.0	0.0017061632303089528	0.0	8.086806685795768	3.2896611303342554E-4
68-69	0.0	0.0017396174112954028	0.0	8.090207860862723	3.3454180986450054E-4
70-71	0.0	0.0017619201986197029	0.0	8.093118374608544	3.3454180986450054E-4
72-73	0.0	0.0017730715922818529	0.0	8.095750103512811	3.679959908509506E-4
74-75	0.0	0.0018121014700993778	0.0	8.097896746792776	3.679959908509506E-4
76-77	0.0	0.001851131347916903	0.0	8.099909573348793	3.679959908509506E-4
78-79	0.0	0.001862282741579053	0.0	8.101520949732974	3.679959908509506E-4
80-81	0.0	0.001873434135241203	0.0	8.10226809310834	3.679959908509506E-4
82-83	0.0	0.001895736922565503	0.0	8.10274202733898	3.791473845131006E-4
84-85	0.0	0.001929191103551953	0.0	8.102942752424898	3.791473845131006E-4
86-87	0.0	0.0019737966782005533	0.0	8.102959479515391	3.847230813441756E-4
88	0.0	0.0020407050401734534	0.0	8.102970630909054	3.902987781752506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7705	0.0	41.514088	1
GTATCAA	13080	0.0	33.368572	1
ATCAACG	14065	0.0	29.37376	3
TCAACGC	14060	0.0	29.352915	4
CAACGCA	14265	0.0	28.83872	5
AACGCAG	14945	0.0	27.585434	6
TATCAAC	15690	0.0	27.032598	2
ACGCAGA	17865	0.0	23.052017	7
CGCAGAG	18275	0.0	22.486696	8
GTACATG	30820	0.0	22.013317	1
TCGGTGG	3890	0.0	21.785357	94
TACATGG	30695	0.0	21.486599	2
ACATGGG	30905	0.0	20.799608	3
CATGGGG	23090	0.0	18.04512	4
GCAGAGT	22870	0.0	17.660892	9
GGACCGA	1420	0.0	16.42191	6
AGAGTAC	19935	0.0	16.144857	10-11
CAGAGTA	22425	0.0	15.921802	10-11
ATGGGGG	13935	0.0	15.408097	5
GTACTAG	2430	0.0	15.390275	1
>>END_MODULE