FastQCFastQC Report
Fri 27 May 2016
SRR2140320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10115493
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83600.038.4080771
GTATCAA141750.031.1279891
TCAACGC146550.028.1088334
ATCAACG148400.027.8148373
CAACGCA150400.027.3057865
AACGCAG158350.026.1992636
TATCAAC168800.025.0485422
ACGCAGA190100.021.5812937
CGCAGAG193850.021.1638078
GTACATG327300.020.5799141
ACCGAAC14900.020.5102088
TACATGG320850.020.3412482
ACATGGG323400.019.6242313
GGACCGA18950.018.7777356
CATGGGG250900.018.1535554
GCAGAGT241100.016.859929
TATACCG8650.016.454945
CCGAACC16800.016.4462999
GTACTAG27450.016.31831
AGGACCG25450.015.2977895
Produced by FastQC (version 0.11.5)