##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140320_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10115493
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.532150632697782	31.0	31.0	34.0	28.0	34.0
2	31.65875454612049	33.0	31.0	34.0	28.0	34.0
3	31.710323560107252	33.0	31.0	34.0	28.0	34.0
4	35.25219017995465	37.0	35.0	37.0	32.0	37.0
5	35.311386899284095	37.0	35.0	37.0	32.0	37.0
6	35.36647447633052	37.0	35.0	37.0	32.0	37.0
7	35.253365011472994	37.0	35.0	37.0	32.0	37.0
8	35.21819490162269	37.0	35.0	37.0	32.0	37.0
9	36.78196505103607	39.0	37.0	39.0	32.0	39.0
10-11	36.7552658580259	39.0	37.0	39.0	32.0	39.0
12-13	36.696923669464255	39.0	37.0	39.0	32.0	39.0
14-15	37.98613661242215	40.0	38.0	41.0	32.0	41.0
16-17	37.99860031537761	40.0	38.0	41.0	32.0	41.0
18-19	38.011409429080715	40.0	38.0	41.0	32.0	41.0
20-21	38.06697214235634	40.0	38.0	41.0	32.0	41.0
22-23	38.04865520712214	40.0	38.0	41.0	32.0	41.0
24-25	38.054201497088144	40.0	38.0	41.0	32.0	41.0
26-27	38.021590218587356	40.0	38.0	41.0	32.0	41.0
28-29	37.99451626546151	40.0	38.0	41.0	32.0	41.0
30-31	37.94351226100814	40.0	37.0	41.0	32.0	41.0
32-33	37.901287655701736	40.0	37.0	41.0	32.0	41.0
34-35	37.853161879926	40.0	37.0	41.0	32.0	41.0
36-37	37.79989386880733	40.0	37.0	41.0	31.5	41.0
38-39	37.720571186010595	40.0	37.0	41.0	31.0	41.0
40-41	37.65570642165912	40.0	37.0	41.0	31.0	41.0
42-43	37.5771187045098	40.0	37.0	41.0	31.0	41.0
44-45	37.45875369286512	40.0	36.5	41.0	31.0	41.0
46-47	37.36096618406027	40.0	36.0	41.0	31.0	41.0
48-49	37.262467546487	40.0	36.0	41.0	31.0	41.0
50-51	37.12960287004161	39.0	36.0	41.0	30.0	41.0
52-53	37.00813325398258	39.0	35.0	41.0	30.0	41.0
54-55	36.871497513803405	39.0	35.0	40.5	30.0	41.0
56-57	36.699233146196406	39.0	35.0	40.0	30.0	41.0
58-59	36.85318353393262	39.0	35.0	40.5	30.5	41.0
60-61	36.92046516412681	39.0	35.0	41.0	31.0	41.0
62-63	36.765763236904434	39.0	35.0	40.5	30.5	41.0
64-65	36.57251540877812	38.0	35.0	40.0	30.0	41.0
66-67	36.314033614006476	38.0	35.0	40.0	30.0	41.0
68-69	36.05230040522855	37.0	34.0	40.0	30.0	41.0
70-71	35.837739944984186	37.0	34.0	39.5	30.0	41.0
72-73	35.61974646053595	36.0	34.0	39.0	30.0	41.0
74-75	35.3654748710005	36.0	34.0	39.0	30.0	41.0
76-77	35.135214882624496	35.5	34.0	38.5	30.0	40.0
78-79	34.84278155422592	35.0	34.0	37.0	30.0	39.0
80-81	34.55991314035733	35.0	34.0	37.0	30.0	39.0
82-83	34.297575993773634	35.0	34.0	36.5	29.5	39.0
84-85	34.08184780537431	35.0	34.0	36.0	29.0	37.0
86-87	33.89561962303601	35.0	34.0	36.0	29.0	37.0
88-89	33.755341995531225	35.0	34.0	35.5	29.0	37.0
90-91	33.64447263051842	35.0	34.0	35.0	29.0	36.0
92-93	33.55596112380867	35.0	33.0	35.0	29.0	36.0
94-95	33.536382694161105	35.0	33.0	35.0	29.5	36.0
96-97	33.54832276156698	35.0	33.0	35.0	30.0	36.0
98-99	33.59710224518051	35.0	33.5	35.0	30.0	36.0
100	33.25062569120777	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	5.0
16	74.0
17	524.0
18	1815.0
19	5900.0
20	15076.0
21	29893.0
22	47904.0
23	66993.0
24	86787.0
25	105899.0
26	121130.0
27	130640.0
28	145129.0
29	172314.0
30	201420.0
31	231777.0
32	285047.0
33	363961.0
34	487220.0
35	668322.0
36	999507.0
37	1747108.0
38	2500560.0
39	1617289.0
40	83197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.787591865270436	20.68574413525866	14.49839370162186	24.02827029784905
2	13.871839958764246	20.91634090399746	42.41964281918835	22.792176318049943
3	17.652861803176574	26.363816375534043	30.46648344277437	25.51683837851502
4	12.097304599983412	18.0858906234229	39.92866190505989	29.888142871533795
5	13.051513729751573	38.40413671009912	35.86388107413214	12.68046848601717
6	29.433661809661675	38.16763058409511	19.527362630768465	12.871344975474749
7	26.331017809118922	32.861713498463544	23.520319130300336	17.286949562117197
8	24.11695603961171	37.05494136568529	21.553798712529385	17.274303882173612
9	25.37295018641207	17.500185112084996	21.548094591138565	35.57877011036437
10-11	23.782400863309594	27.139336446413548	29.331103617763272	19.747159072513586
12-13	24.43286066016729	24.69375283816198	29.231020787295197	21.642365714375533
14-15	21.75207915028935	26.285739678169833	26.59324330342656	25.368937868114255
16-17	21.581364091201984	29.306349415677868	27.246170770705735	21.866115722414413
18-19	21.67656246910651	29.04051742887083	28.314253514443323	20.968666587579335
20-21	22.61443023947917	28.06722541940831	28.015430312384044	21.302914028728477
22-23	22.31145626123463	27.9628577606503	27.803612664284465	21.922073313830605
24-25	22.06215007133284	28.127242544884588	27.86964361426526	21.94096376951731
26-27	21.98793121937878	28.28397744772115	27.73206505451318	21.996026278386896
28-29	21.89640674098735	28.415204947638696	27.776190747968887	21.91219756340507
30-31	22.275392752558236	28.140578256519944	27.76084540197523	21.823183588946588
32-33	22.030119875671474	28.239732173700517	27.69997316305719	22.03017478757082
34-35	22.110670411569018	28.331643197399508	27.83147618782087	21.726210203210602
36-37	22.090647026445716	28.165702583503233	27.833757314448288	21.909893075602756
38-39	21.97769700494035	28.263151497124305	27.892906612683838	21.86624488525151
40-41	22.190510881847338	28.08642686145718	27.820848925087528	21.902213331607957
42-43	21.97896013456674	28.10257715022856	27.817730358583493	22.100732356621208
44-45	22.032056705018512	28.183893469005884	27.79831631048163	21.985733515493973
46-47	21.96565654515149	28.10717763849822	27.736011583151775	22.191154233198514
48-49	21.903494173912854	28.146055114733176	27.856198772030876	22.094251939323094
50-51	21.903736504993017	28.146758533959993	27.78153275569908	22.167972205347912
52-53	22.002717609020266	28.116289361372637	27.71913881798526	22.161854211621836
54-55	21.95046535558945	27.994680472072016	27.841391590416105	22.21346258192243
56-57	22.02223915290148	28.04935394687068	27.818153910176786	22.110252990051055
58-59	21.983568611137176	28.194747635112787	27.859831390468155	21.961852363281885
60-61	22.133644143290905	28.094019005512155	27.819879209371855	21.95245764182508
62-63	22.07011927224333	28.121991689570248	27.743057923346235	22.064831114840192
64-65	21.94064363960483	28.08393094780634	27.826571212714228	22.148854199874606
66-67	22.046209246752646	28.11730416736971	27.704844601542234	22.13164198433541
68-69	21.95394494555464	28.063039605125383	27.814323713577068	22.16869173574291
70-71	21.92703844806898	27.879333067605195	27.87374510573905	22.319883378586777
72-73	22.008295631253187	27.985933733534146	27.79948313727297	22.206287497939698
74-75	21.82492976764522	28.076595896685458	27.9038313636656	22.194642972003724
76-77	21.941657820710315	28.009318676447197	27.80402169703463	22.245001805807853
78-79	21.92007399602169	28.05753711889415	27.74320347121276	22.279185413871403
80-81	21.954207183443895	28.07817664122649	27.82093814670729	22.146678028622325
82-83	21.988470972696135	28.068662483128154	27.965414437932058	21.977452106243657
84-85	22.106229871417487	28.078275924300023	27.771181912219745	22.04431229206275
86-87	21.831082927753965	28.233335865161337	27.879003290727212	22.056577916357494
88-89	21.91277428250407	28.114334920020944	27.864096127482323	22.10879466999266
90-91	21.972030214686992	28.16624256962702	27.865367557106964	21.996359658579028
92-93	22.015474343518395	28.156276522247005	27.877168240792756	21.951080893441848
94-95	21.789094898600496	28.29325413946512	27.898021440697505	22.01962952123688
96-97	21.778997732554895	28.426846769553855	27.99314257393963	21.80101292395162
98-99	22.860856446771745	30.316844051679396	28.15029843401725	18.672001067531603
100	21.854355874449116	31.40723174730918	21.881940110490724	24.856472267750984
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2998.0
1	2276.5
2	1705.0
3	1874.0
4	1877.0
5	1977.0
6	2280.0
7	2680.0
8	3062.0
9	3486.5
10	3972.0
11	4379.5
12	4833.5
13	5503.0
14	6416.5
15	7606.5
16	9124.5
17	11045.5
18	13449.5
19	16559.0
20	20793.0
21	26443.5
22	34152.0
23	44053.0
24	56852.5
25	73957.0
26	95375.0
27	118428.0
28	141840.5
29	168651.5
30	195399.0
31	220518.5
32	246819.0
33	270817.5
34	295180.0
35	318848.0
36	342191.0
37	366599.0
38	381777.5
39	391015.0
40	404315.0
41	416322.5
42	422283.5
43	427016.0
44	431739.0
45	437405.5
46	441085.0
47	440492.0
48	436366.5
49	427265.0
50	416041.0
51	403833.5
52	387710.0
53	365822.0
54	342439.5
55	316733.0
56	290085.5
57	262174.5
58	232813.5
59	203110.5
60	169310.0
61	137149.0
62	109134.0
63	85431.5
64	65967.5
65	50022.5
66	37551.0
67	28636.5
68	22423.5
69	17235.0
70	13380.0
71	10427.5
72	8072.5
73	6176.0
74	4684.0
75	3566.0
76	2694.5
77	2059.0
78	1557.0
79	1144.5
80	843.5
81	648.0
82	514.0
83	366.0
84	258.5
85	196.5
86	156.0
87	120.5
88	77.0
89	46.0
90	34.0
91	31.0
92	26.5
93	19.5
94	14.5
95	11.0
96	8.5
97	8.5
98	6.0
99	10.5
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.4828738451007776E-4
6	0.0
7	9.885825634005184E-6
8	0.0
9	0.0
10-11	2.471456408501296E-5
12-13	1.1368699479105962E-4
14-15	2.471456408501296E-5
16-17	1.0874408197405703E-4
18-19	9.193817839624821E-4
20-21	4.0062899741802034E-4
22-23	0.0
24-25	8.918764768917035E-5
26-27	1.587263952769374E-4
28-29	0.0
30-31	0.0
32-33	6.489583893371944E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	5.8615988064932234E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.3274709000276838E-4
76-77	6.102763581563039E-6
78-79	4.19924918659617E-4
80-81	3.7517450304072676E-5
82-83	3.799607677841904E-5
84-85	4.103032532111519E-4
86-87	9.088605070506802E-5
88-89	1.9734916652539756E-5
90-91	2.0018737538335882E-5
92-93	5.486893369746435E-4
94-95	3.579931153793223E-4
96-97	3.5882733538197944E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4376.0
20-21	8342.0
22-23	9246.0
24-25	10256.0
26-27	13237.0
28-29	17760.0
30-31	28440.0
32-33	33930.0
34-35	43832.0
36-37	63345.0
38-39	73889.0
40-41	71403.0
42-43	74968.0
44-45	84078.0
46-47	86802.0
48-49	87237.0
50-51	85382.0
52-53	83163.0
54-55	88659.0
56-57	91930.0
58-59	91485.0
60-61	91927.0
62-63	88192.0
64-65	91965.0
66-67	96340.0
68-69	97447.0
70-71	96160.0
72-73	92544.0
74-75	92646.0
76-77	95073.0
78-79	99372.0
80-81	101837.0
82-83	97393.0
84-85	96407.0
86-87	99561.0
88-89	106218.0
90-91	111090.0
92-93	115479.0
94-95	134906.0
96-97	326952.0
98-99	589528.0
100-101	6242696.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.66295624606774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.00739415667582	73.57614328749798
2	7.150734118291907	11.822016430952553
3	1.9759451376281527	4.900123993691588
4	0.7904771182992778	2.613727017739637
5	0.4051187933603818	1.67441585450045
6	0.20802124361809735	1.0317390575673226
7	0.13529210993193777	0.7828552034619369
8	0.08675102690578368	0.5736877073131393
9	0.05892307032124434	0.4383679665464095
>10	0.17701197532150678	2.238797238388631
>50	0.003181487257606801	0.17518465287401588
>100	0.001106389216391686	0.14422294725890084
>500	4.3373171893533485E-5	0.028718642207505
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9657476902015553E-5	0.0	9.885825634005184E-6	0.0
3	0.0	2.9657476902015553E-5	0.0	1.9771651268010367E-5	0.0
4	0.0	6.920077943803629E-5	0.0	1.9771651268010367E-5	0.0
5	0.0	6.920077943803629E-5	0.0	3.9543302536020735E-5	0.0
6	0.0	6.920077943803629E-5	0.0	3.9543302536020735E-5	0.0
7	0.0	6.920077943803629E-5	0.0	3.9543302536020735E-5	0.0
8	0.0	8.897243070604666E-5	0.0	4.942912817002592E-5	0.0
9	0.0	1.4828738451007776E-4	0.0	5.9314953804031106E-5	0.0
10-11	0.0	1.4828738451007776E-4	0.0	6.920077943803629E-5	0.0
12-13	0.0	1.4828738451007776E-4	0.0	7.908660507204147E-5	4.942912817002592E-6
14-15	0.0	1.9771651268010368E-4	0.0	1.0380116915705443E-4	1.4828738451007775E-5
16-17	0.0	1.9771651268010368E-4	0.0	1.0874408197405702E-4	2.9657476902015553E-5
18-19	0.0	2.1748816394811404E-4	0.0	1.1862990760806221E-4	2.9657476902015553E-5
20-21	0.0	2.3725981521612442E-4	0.0	1.5817321014408297E-4	3.4600389719018146E-5
22-23	0.0	2.6691729211814E-4	0.0	2.3725981521612442E-4	4.942912817002592E-5
24-25	0.0	3.015176818371581E-4	0.0	3.707184612751944E-4	5.9314953804031106E-5
26-27	0.0	3.064605946541607E-4	0.0	6.771790559293551E-4	5.9314953804031106E-5
28-29	0.0	3.064605946541607E-4	0.0	0.002624686705828376	5.9314953804031106E-5
30-31	0.0	3.460038971901814E-4	0.0	0.00842766635298942	5.9314953804031106E-5
32-33	0.0	3.460038971901814E-4	0.0	0.02404232794190061	5.9314953804031106E-5
34-35	0.0	3.9543302536020736E-4	0.0	0.04076914491463737	6.920077943803629E-5
36-37	0.0	4.349763278962281E-4	0.0	0.059468184101358186	6.920077943803629E-5
38-39	0.0	4.5474797916423843E-4	0.0	0.07852805592372017	6.920077943803629E-5
40-41	0.0	5.041771073342644E-4	0.0	0.0963175991521125	7.908660507204147E-5
42-43	0.0	5.190058457852722E-4	0.0	0.11278738465836514	7.908660507204147E-5
44-45	0.0	5.58549148321293E-4	0.0	0.12647925316146233	7.908660507204147E-5
46-47	0.0	5.832637124063058E-4	0.0	0.13971637368539525	8.897243070604666E-5
48-49	0.0	5.931495380403109E-4	0.0	0.15115427394393927	1.0874408197405702E-4
50-51	0.0	6.574074046613447E-4	0.0	0.16129713104442858	1.0874408197405702E-4
52-53	0.0	6.623503174783473E-4	0.0	0.17042174810461536	1.2851573324206738E-4
54-55	0.0	6.623503174783473E-4	0.0	0.1790718455343699	1.5323029732708035E-4
56-57	0.0	6.623503174783473E-4	0.0	0.1876725138359544	1.6311612296108553E-4
58-59	0.0	6.623503174783473E-4	0.0	0.19667850098853312	1.6805903577808812E-4
60-61	0.0	6.920077943803628E-4	0.0	0.20436473041897218	1.7794486141209332E-4
62-63	0.0	7.068365328313707E-4	0.0	0.21120572175770375	1.878306870460985E-4
64-65	0.0	7.908660507204147E-4	0.0	0.21559502833920208	1.878306870460985E-4
66-67	0.0	7.908660507204147E-4	0.0	0.21901058109575083	1.878306870460985E-4
68-69	0.0	8.0075187635442E-4	0.0	0.22269799405723478	1.927735998631011E-4
70-71	0.0	8.106377019884251E-4	0.0	0.2260048027318095	1.9771651268010368E-4
72-73	0.0	8.106377019884251E-4	0.0	0.22856523157101685	2.1748816394811404E-4
74-75	0.0	8.155806148054277E-4	0.0	0.23078459942585103	2.1748816394811404E-4
76-77	0.0	8.551239173414484E-4	0.0	0.2329841956294172	2.2737398958211922E-4
78-79	0.0	8.699526557924562E-4	0.0	0.23465984307438104	2.3725981521612442E-4
80-81	0.0	8.699526557924562E-4	0.0	0.23539139417129745	2.42202728033127E-4
82-83	0.0	8.699526557924562E-4	0.0	0.23582637049919367	2.718602049351426E-4
84-85	0.0	8.748955686094588E-4	0.0	0.23603891575032476	2.7680311775214516E-4
86-87	0.0	8.996101326944717E-4	0.0	0.2360932877913118	2.7680311775214516E-4
88	0.0	9.68810912132508E-4	0.0	0.23611305944257982	2.7680311775214516E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8360	0.0	38.408077	1
GTATCAA	14175	0.0	31.127989	1
TCAACGC	14655	0.0	28.108833	4
ATCAACG	14840	0.0	27.814837	3
CAACGCA	15040	0.0	27.305786	5
AACGCAG	15835	0.0	26.199263	6
TATCAAC	16880	0.0	25.048542	2
ACGCAGA	19010	0.0	21.581293	7
CGCAGAG	19385	0.0	21.163807	8
GTACATG	32730	0.0	20.579914	1
ACCGAAC	1490	0.0	20.510208	8
TACATGG	32085	0.0	20.341248	2
ACATGGG	32340	0.0	19.624231	3
GGACCGA	1895	0.0	18.777735	6
CATGGGG	25090	0.0	18.153555	4
GCAGAGT	24110	0.0	16.85992	9
TATACCG	865	0.0	16.45494	5
CCGAACC	1680	0.0	16.446299	9
GTACTAG	2745	0.0	16.3183	1
AGGACCG	2545	0.0	15.297789	5
>>END_MODULE