FastQCFastQC Report
Fri 27 May 2016
SRR2140319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10103834
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76200.034.6058921
GTATCAA126900.031.917921
TCAACGC132900.028.9848334
ATCAACG133450.028.8337573
CAACGCA135850.028.3243665
AACGCAG143150.026.9978456
TATCAAC149650.026.6710072
ACGCAGA172600.022.2692037
CGCAGAG176800.021.6924578
GTACATG292350.020.2045841
TACATGG290300.019.6351072
ACATGGG292500.018.8671743
CATGGGG216150.017.3333574
GCAGAGT225450.016.9552869
AGAGTAC195300.015.37091810-11
GTGTAGC58700.014.734681
GTACTAG25200.014.5661131
CAGAGTA222500.014.18410-11
ACCGAAC10950.013.8712488
GGACCGA13200.013.4245696
Produced by FastQC (version 0.11.5)