##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140319_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10103834
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.828783608281768	33.0	31.0	34.0	30.0	34.0
2	31.94944493347773	34.0	31.0	34.0	30.0	34.0
3	32.00046705042858	34.0	31.0	34.0	30.0	34.0
4	35.51267241722301	37.0	35.0	37.0	33.0	37.0
5	35.57412126921325	37.0	35.0	37.0	33.0	37.0
6	35.624851417788534	37.0	35.0	37.0	33.0	37.0
7	35.519786944243144	37.0	35.0	37.0	33.0	37.0
8	35.49199086208265	37.0	35.0	37.0	33.0	37.0
9	37.10942519443609	39.0	37.0	39.0	33.0	39.0
10-11	37.090025182519824	39.0	37.0	39.0	33.0	39.0
12-13	37.04020166997993	39.0	37.0	39.0	33.0	39.0
14-15	38.40653740946259	40.0	38.0	41.0	33.0	41.0
16-17	38.41915098763499	40.0	38.0	41.0	33.0	41.0
18-19	38.43487244544991	40.0	38.0	41.0	33.0	41.0
20-21	38.488376318624525	40.0	38.0	41.0	33.0	41.0
22-23	38.47031034808197	40.0	38.0	41.0	33.0	41.0
24-25	38.481792047054704	40.0	38.0	41.0	33.0	41.0
26-27	38.4620816477067	40.0	38.0	41.0	33.0	41.0
28-29	38.435409115181045	40.0	38.0	41.0	33.0	41.0
30-31	38.38626908737136	40.0	38.0	41.0	33.0	41.0
32-33	38.34544808737957	40.0	38.0	41.0	33.0	41.0
34-35	38.29372720315757	40.0	38.0	41.0	33.0	41.0
36-37	38.23702814997745	40.0	38.0	41.0	33.0	41.0
38-39	38.160556236892006	40.0	38.0	41.0	33.0	41.0
40-41	38.09942487717052	40.0	38.0	41.0	33.0	41.0
42-43	38.018080697172586	40.0	38.0	41.0	32.5	41.0
44-45	37.90583119902839	40.0	37.0	41.0	32.0	41.0
46-47	37.81229116683507	40.0	37.0	41.0	32.0	41.0
48-49	37.71880319040727	40.0	37.0	41.0	32.0	41.0
50-51	37.588783363153695	40.0	37.0	41.0	31.0	41.0
52-53	37.47290692993075	40.0	36.0	41.0	31.0	41.0
54-55	37.34048177251222	39.5	36.0	41.0	31.0	41.0
56-57	37.17179456538684	39.0	36.0	41.0	31.0	41.0
58-59	37.316287352194664	39.0	36.0	41.0	31.0	41.0
60-61	37.37489063662584	39.0	36.0	41.0	31.0	41.0
62-63	37.22132221817466	39.0	35.0	41.0	31.0	41.0
64-65	37.023463747422376	39.0	35.0	41.0	31.0	41.0
66-67	36.764232098731476	38.0	35.0	40.0	31.0	41.0
68-69	36.49925077511242	37.5	35.0	40.0	31.0	41.0
70-71	36.2720084916449	37.0	35.0	40.0	31.0	41.0
72-73	36.03905025328993	37.0	35.0	39.0	31.0	41.0
74-75	35.77608150363432	36.0	35.0	39.0	31.0	41.0
76-77	35.52786927051747	36.0	34.0	39.0	31.0	40.5
78-79	35.22093828838375	35.5	34.0	37.5	30.5	39.5
80-81	34.924100928146174	35.0	34.0	37.0	30.0	39.0
82-83	34.64668056065973	35.0	34.0	37.0	30.0	39.0
84-85	34.41308871432193	35.0	34.0	36.0	30.0	38.0
86-87	34.213080298559746	35.0	34.0	36.0	30.0	37.0
88-89	34.05637154800969	35.0	34.0	36.0	30.0	37.0
90-91	33.934350429754645	35.0	34.0	35.0	30.0	36.5
92-93	33.833341541312656	35.0	34.0	35.0	30.0	36.0
94-95	33.798013854657796	35.0	34.0	35.0	30.0	36.0
96-97	33.79154061656909	35.0	34.0	35.0	30.0	36.0
98-99	33.81773082197278	35.0	34.0	35.0	30.0	36.0
100	33.480594930314105	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	4.0
16	38.0
17	310.0
18	1333.0
19	4110.0
20	10483.0
21	21633.0
22	35567.0
23	50203.0
24	66010.0
25	82845.0
26	95598.0
27	102164.0
28	117248.0
29	141276.0
30	166944.0
31	195430.0
32	246078.0
33	320247.0
34	433309.0
35	609032.0
36	931652.0
37	1714537.0
38	2712246.0
39	1927773.0
40	117760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.773027347836475	20.849204371330725	14.402107160509564	23.975661120323235
2	13.812331041859952	21.123674438831834	42.592465394819435	22.471529124488786
3	17.592430754503685	26.586254287233935	30.62676009918611	25.19455485907627
4	11.998653184523816	18.25736646108794	40.23576594785702	29.508214406531224
5	12.958209168236287	38.34180917136381	36.08802770516772	12.611953955232174
6	29.063393163426877	38.50654118030838	19.73311319247723	12.69695246378751
7	26.005930026166308	32.92601600540943	23.923156298886145	17.144897669538118
8	23.70707990649886	37.36281692672306	21.826051378120425	17.10405178865765
9	24.995184996111377	17.78419954247071	21.962850933615893	35.257764527802024
10-11	23.48539759397215	27.367177491033974	29.632625009631248	19.514799905362633
12-13	24.170954894993706	24.870749418314237	29.53342512037275	21.424870566319303
14-15	21.51091890239856	26.484561821918746	26.832405112852488	25.172114162830212
16-17	21.397519187163315	29.502533471109533	27.535342925310815	21.564604416416337
18-19	21.455063030614106	29.218103472763207	28.517768814541068	20.80906468208162
20-21	22.398574439393744	28.277392975147304	28.24761293011433	21.076419655344623
22-23	22.08140117157319	28.18147058771075	28.03027795981156	21.7068502809045
24-25	21.842534096007945	28.33779082844169	28.093016518287577	21.726658557262787
26-27	21.819323158946172	28.490576297881354	27.955630098133838	21.734470445038642
28-29	21.730335211245077	28.582972113355794	27.999899068514644	21.68679360688448
30-31	22.01641879648732	28.332621564558817	27.99146016789185	21.65949947106201
32-33	21.785510975055686	28.456543440596704	27.965889901655792	21.79205568269182
34-35	21.926504684395407	28.432316095185957	28.02858735577819	21.61259186464045
36-37	21.90406204399944	28.293354200185355	28.05054430906024	21.75203944675497
38-39	21.778026691928577	28.4177807525177	28.139983050591972	21.664209504961754
40-41	21.98853200882946	28.238144674756754	28.048863393147816	21.724459923265968
42-43	21.796067171520622	28.314477398845373	28.020758232449612	21.868697197184396
44-45	21.856201110975487	28.380564453071834	28.015484045863264	21.74775039008942
46-47	21.807785349314145	28.284457101465442	27.938937930157227	21.968819619063186
48-49	21.79629630991785	28.27522151242646	28.055860610230077	21.872621567425618
50-51	21.75550395122861	28.334347284166256	28.008365624311075	21.901783140294054
52-53	21.836426738936336	28.31421464403558	27.938995424106324	21.91036319292177
54-55	21.8618088966331	28.216407427232564	28.00153020101086	21.920253475123477
56-57	21.894596738824056	28.293160846299426	27.976326383137568	21.83591603173895
58-59	21.834062550871568	28.345727843015784	28.02062778105926	21.79958182505339
60-61	21.950617788039324	28.285071353051173	27.966399484994486	21.797911373915017
62-63	21.87722460643178	28.355502589302557	27.928462174471935	21.83881062979373
64-65	21.835730646537414	28.281119391982806	27.975417636034226	21.907732325445554
66-67	21.89197431484584	28.33116769003406	27.864556493696284	21.912301501423816
68-69	21.844215962560064	28.265974025734035	27.973427590339593	21.916382421366308
70-71	21.858388316186108	28.0754407822679	28.004914918873364	22.061255982672623
72-73	21.913363468114465	28.136484144658752	28.013721046692464	21.93643134053432
74-75	21.74759287504852	28.264187167519484	28.032182957004213	21.95603700042778
76-77	21.87242800215031	28.2473133938206	27.940770596108035	21.939488007921053
78-79	21.82842522317405	28.22171525005369	27.92401362396042	22.025845902811835
80-81	21.84449959868746	28.23586166407313	27.947752341154423	21.971886396084987
82-83	21.828911392069823	28.218191048899715	28.11750887863211	21.835388680398353
84-85	21.968070052457072	28.246932611183702	27.95842786872785	21.82656946763138
86-87	21.735749319438657	28.299895402511734	28.041639786350313	21.922715491699297
88-89	21.824253737622705	28.25020097529654	27.993101466310588	21.932443820770168
90-91	21.81437298093046	28.351743985458633	28.018578653753366	21.81530437985754
92-93	21.852248893899926	28.30851322937059	28.045757512109205	21.793480364620283
94-95	21.662152278171387	28.43868987889288	28.06277548317458	21.836382359761156
96-97	21.75602004348559	28.554969160053645	28.12461085023919	21.56439994622157
98-99	22.827602393419795	30.498033285974635	28.279530328219373	18.3948339923862
100	21.828318232507364	31.452134839193018	22.087088989300387	24.632457938999234
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2794.0
1	2107.0
2	1591.0
3	1752.0
4	1715.5
5	1816.0
6	2119.5
7	2486.5
8	2827.5
9	3234.5
10	3727.0
11	4167.0
12	4625.5
13	5193.0
14	6007.5
15	7225.5
16	8903.5
17	10931.5
18	13514.5
19	17046.5
20	21566.5
21	27746.0
22	36407.0
23	47623.0
24	61744.0
25	79245.0
26	101132.5
27	126794.0
28	153641.5
29	182591.5
30	210086.0
31	235008.0
32	261224.0
33	285920.0
34	309735.5
35	331932.5
36	353913.0
37	374195.5
38	385914.0
39	393487.0
40	403167.5
41	410472.0
42	414321.5
43	419161.0
44	423911.5
45	428355.0
46	429601.5
47	425869.5
48	419586.5
49	410522.0
50	399515.0
51	385016.5
52	365568.0
53	343080.5
54	319468.5
55	294215.5
56	269223.5
57	243507.0
58	216214.5
59	189274.5
60	159173.0
61	129163.5
62	102616.5
63	80165.0
64	61775.5
65	46984.0
66	35872.0
67	28031.0
68	21941.5
69	16752.5
70	12959.0
71	9876.0
72	7391.5
73	5546.5
74	4188.5
75	3177.5
76	2430.5
77	1887.5
78	1456.5
79	1123.0
80	867.0
81	644.5
82	481.5
83	332.5
84	237.0
85	178.5
86	146.0
87	113.5
88	72.5
89	49.0
90	36.0
91	30.0
92	26.5
93	16.0
94	7.5
95	6.0
96	5.5
97	3.5
98	2.0
99	5.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.484584960520927E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.4743082675348782E-5
12-13	1.0886956377153464E-4
14-15	2.4743082675348782E-5
16-17	8.412648109618587E-5
18-19	9.204426755229746E-4
20-21	4.3568288365826283E-4
22-23	0.0
24-25	6.940431319053265E-5
26-27	1.7861608259207658E-4
28-29	0.0
30-31	0.0
32-33	4.988341746504245E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	3.7095748419177926E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.3718511765741263E-4
76-77	1.2060066124136553E-5
78-79	4.3305859526770644E-4
80-81	4.937709559479773E-5
82-83	2.4984712479051878E-5
84-85	4.8021759417430347E-4
86-87	1.5343413401870657E-4
88-89	3.2363161272428885E-5
90-91	1.9677438249411138E-5
92-93	7.449101684893687E-4
94-95	5.332856661453518E-4
96-97	3.3749157390297504E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3226.0
20-21	6150.0
22-23	6827.0
24-25	7782.0
26-27	10398.0
28-29	14552.0
30-31	24738.0
32-33	30081.0
34-35	39598.0
36-37	58829.0
38-39	66564.0
40-41	65586.0
42-43	70585.0
44-45	79379.0
46-47	82353.0
48-49	82240.0
50-51	79984.0
52-53	79614.0
54-55	84774.0
56-57	87996.0
58-59	87619.0
60-61	87600.0
62-63	84290.0
64-65	88030.0
66-67	93121.0
68-69	93560.0
70-71	93075.0
72-73	90256.0
74-75	90064.0
76-77	93361.0
78-79	96299.0
80-81	97101.0
82-83	92391.0
84-85	91508.0
86-87	94660.0
88-89	100513.0
90-91	104775.0
92-93	108115.0
94-95	125948.0
96-97	320922.0
98-99	595680.0
100-101	6393690.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.34838572754157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.09711492710453	74.26100702155411
2	7.3030268199428745	12.1739099273436
3	1.9150388529144344	4.788461909878231
4	0.7365144885338382	2.4554917473696536
5	0.3554457320593397	1.4812913990445118
6	0.17745499362111739	0.8874352354566792
7	0.12192087386929053	0.7113335616447599
8	0.07489905849550405	0.49941724944903765
9	0.04845284465680411	0.3634619747446765
>10	0.16686020210527458	2.0921739145803366
>50	0.0026141950672552558	0.142027762277131
>100	5.526762552348328E-4	0.07351597926233672
>500	8.346957998330799E-5	0.04391579780250186
>1k	2.0865794568395093E-5	0.026556519592407065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.897233070139513E-6
2	0.0	3.958893228055805E-5	0.0	0.0	9.897233070139513E-6
3	0.0	3.958893228055805E-5	0.0	0.0	9.897233070139513E-6
4	0.0	9.897233070139513E-5	0.0	9.897233070139513E-6	9.897233070139513E-6
5	0.0	9.897233070139513E-5	0.0	9.897233070139513E-6	9.897233070139513E-6
6	0.0	9.897233070139513E-5	0.0	9.897233070139513E-6	1.9794466140279026E-5
7	0.0	9.897233070139513E-5	0.0	9.897233070139513E-6	1.9794466140279026E-5
8	9.897233070139513E-6	1.0886956377153464E-4	0.0	3.958893228055805E-5	1.9794466140279026E-5
9	9.897233070139513E-6	2.1773912754306928E-4	0.0	8.907509763125562E-5	1.9794466140279026E-5
10-11	9.897233070139513E-6	2.1773912754306928E-4	0.0	8.907509763125562E-5	1.9794466140279026E-5
12-13	9.897233070139513E-6	2.1773912754306928E-4	0.0	1.0392094723646488E-4	1.9794466140279026E-5
14-15	1.9794466140279026E-5	3.66197623595162E-4	0.0	1.0886956377153464E-4	2.969169921041854E-5
16-17	1.9794466140279026E-5	3.7114624013023177E-4	0.0	1.2866402991181368E-4	3.958893228055805E-5
18-19	1.9794466140279026E-5	4.008379393406503E-4	0.0	1.484584960520927E-4	3.958893228055805E-5
20-21	1.9794466140279026E-5	4.2558102201599907E-4	0.0	1.7320157872744148E-4	4.453754881562781E-5
22-23	2.969169921041854E-5	5.196047361823244E-4	0.0	2.721739094288366E-4	5.443478188576732E-5
24-25	3.958893228055805E-5	5.789881346031615E-4	0.0	4.8496442043683617E-4	5.938339842083708E-5
26-27	3.958893228055805E-5	6.185770668837196E-4	0.0	9.847746904788814E-4	6.433201495590683E-5
28-29	3.958893228055805E-5	6.235256834187893E-4	0.0	0.0037065137847672475	6.928063149097659E-5
30-31	4.9486165350697564E-5	7.126007810500449E-4	0.0	0.013494877291135226	7.422924802604635E-5
32-33	4.9486165350697564E-5	7.32395247190324E-4	0.0	0.038940663514463916	7.91778645611161E-5
34-35	6.433201495590683E-5	8.660078936372074E-4	0.0	0.06578195960068228	8.907509763125562E-5
36-37	8.412648109618585E-5	9.897233070139512E-4	0.0	0.09547365881110081	9.897233070139513E-5
38-39	8.907509763125562E-5	9.996205400840908E-4	0.0	0.12280486793429109	9.897233070139513E-5
40-41	8.907509763125562E-5	0.0010590039385049278	0.0	0.14807250396235727	1.0886956377153464E-4
42-43	8.907509763125562E-5	0.001098592870785486	0.0	0.17023735742293472	1.0886956377153464E-4
44-45	8.907509763125562E-5	0.0012124110510920905	0.0	0.1863154125453763	1.0886956377153464E-4
46-47	8.907509763125562E-5	0.0012767430660479973	0.0	0.1986919024995858	1.2866402991181365E-4
48-49	8.907509763125562E-5	0.0012767430660479973	0.0	0.20810417114928847	1.484584960520927E-4
50-51	8.907509763125562E-5	0.0014153043290299505	0.0	0.215447918087332	1.484584960520927E-4
52-53	8.907509763125562E-5	0.0014153043290299505	0.0	0.22100521445621535	1.583557291222322E-4
54-55	8.907509763125562E-5	0.0014202529455650203	0.0	0.2257558863298823	1.7320157872744148E-4
56-57	8.907509763125562E-5	0.0014449960282403689	0.0	0.23044717480512844	1.7815019526251123E-4
58-59	8.907509763125562E-5	0.0014598418778455783	0.0	0.23569270833230238	1.7815019526251123E-4
60-61	8.907509763125562E-5	0.0014746877274507877	0.0	0.23996336440206756	1.8804742833265076E-4
62-63	8.907509763125562E-5	0.001504379426661206	0.0	0.2441152536749911	1.9794466140279026E-4
64-65	8.907509763125562E-5	0.0016825296219237173	0.0	0.2473417516558566	1.9794466140279026E-4
66-67	8.907509763125562E-5	0.0017023240880639963	0.0	0.2503950480579946	1.9794466140279026E-4
68-69	9.897233070139513E-5	0.0017716047195549729	0.0	0.25385907963254345	1.9794466140279026E-4
70-71	9.897233070139513E-5	0.0018111936518355309	0.0	0.257021245598453	2.2268774407813906E-4
72-73	9.897233070139513E-5	0.0018210908849056705	0.0	0.2595598858809438	2.5732805982362736E-4
74-75	9.897233070139513E-5	0.0018557312006511587	0.0	0.2619946052161981	2.5732805982362736E-4
76-77	9.897233070139513E-5	0.0019448062982824143	0.0	0.26428086605540035	2.5732805982362736E-4
78-79	9.897233070139513E-5	0.001989343847098042	0.0	0.2661118541733762	2.5732805982362736E-4
80-81	9.897233070139513E-5	0.0020041896967032515	0.0	0.26687888973631196	2.5732805982362736E-4
82-83	9.897233070139513E-5	0.0020091383132383213	0.0	0.2673688027732839	2.721739094288366E-4
84-85	9.897233070139513E-5	0.0020239841628435307	0.0	0.26754695296854636	2.7712252596390636E-4
86-87	9.897233070139513E-5	0.0020685217116591585	0.0	0.2676310794496426	2.7712252596390636E-4
88	9.897233070139513E-5	0.0021180078770098557	0.0	0.2676310794496426	2.870197590340459E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7620	0.0	34.605892	1
GTATCAA	12690	0.0	31.91792	1
TCAACGC	13290	0.0	28.984833	4
ATCAACG	13345	0.0	28.833757	3
CAACGCA	13585	0.0	28.324366	5
AACGCAG	14315	0.0	26.997845	6
TATCAAC	14965	0.0	26.671007	2
ACGCAGA	17260	0.0	22.269203	7
CGCAGAG	17680	0.0	21.692457	8
GTACATG	29235	0.0	20.204584	1
TACATGG	29030	0.0	19.635107	2
ACATGGG	29250	0.0	18.867174	3
CATGGGG	21615	0.0	17.333357	4
GCAGAGT	22545	0.0	16.955286	9
AGAGTAC	19530	0.0	15.370918	10-11
GTGTAGC	5870	0.0	14.73468	1
GTACTAG	2520	0.0	14.566113	1
CAGAGTA	22250	0.0	14.184	10-11
ACCGAAC	1095	0.0	13.871248	8
GGACCGA	1320	0.0	13.424569	6
>>END_MODULE