FastQCFastQC Report
Fri 27 May 2016
SRR2140318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6902030
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56100.035.2670631
GTATCAA90350.031.2560861
TCAACGC90600.028.976754
ATCAACG92150.028.5352273
CAACGCA92600.028.3509035
AACGCAG98250.026.6773246
TATCAAC107200.025.3967042
ACGCAGA118200.022.2104437
CGCAGAG120850.021.6184718
GTACATG200500.019.6511421
TACATGG197450.019.3766082
ACATGGG202100.018.324163
TATACCG6000.016.9101055
GCAGAGT158100.016.4981949
CATGGGG151750.016.1579254
GGACCGA11150.015.9241986
ACCGAAC9600.015.8531088
AGGACCG14000.015.0983065
AGAGTAC132400.014.68772610-11
CAGAGTA153400.014.57857310-11
Produced by FastQC (version 0.11.5)