##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140318_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6902030
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83829279212058	33.0	31.0	34.0	30.0	34.0
2	31.961398603019692	34.0	31.0	34.0	30.0	34.0
3	32.013897650401404	34.0	31.0	34.0	30.0	34.0
4	35.525668245429245	37.0	35.0	37.0	33.0	37.0
5	35.583084686679136	37.0	35.0	37.0	33.0	37.0
6	35.634766438279755	37.0	35.0	37.0	33.0	37.0
7	35.53253115387792	37.0	35.0	37.0	33.0	37.0
8	35.501019844886216	37.0	35.0	37.0	33.0	37.0
9	37.12313333903214	39.0	37.0	39.0	33.0	39.0
10-11	37.10108236272517	39.0	37.0	39.0	33.0	39.0
12-13	37.05284937909572	39.0	37.0	39.0	33.0	39.0
14-15	38.41975440558792	40.0	38.0	41.0	33.0	41.0
16-17	38.43265046660186	40.0	38.0	41.0	33.0	41.0
18-19	38.449319258247215	40.0	38.0	41.0	33.0	41.0
20-21	38.500460893855276	40.0	38.0	41.0	33.0	41.0
22-23	38.48247316660654	40.0	38.0	41.0	33.0	41.0
24-25	38.491040353055595	40.0	38.0	41.0	33.0	41.0
26-27	38.469112263766135	40.0	38.0	41.0	33.0	41.0
28-29	38.444355403623966	40.0	38.0	41.0	33.0	41.0
30-31	38.394525765530176	40.0	38.0	41.0	33.0	41.0
32-33	38.353240741759706	40.0	38.0	41.0	33.0	41.0
34-35	38.29955236600985	40.0	38.0	41.0	33.0	41.0
36-37	38.24287762701533	40.0	38.0	41.0	33.0	41.0
38-39	38.16611650042958	40.0	38.0	41.0	33.0	41.0
40-41	38.10167061683101	40.0	38.0	41.0	33.0	41.0
42-43	38.01958768081474	40.0	38.0	41.0	32.5	41.0
44-45	37.91039521057476	40.0	37.0	41.0	32.0	41.0
46-47	37.81261717513132	40.0	37.0	41.0	32.0	41.0
48-49	37.71774787507016	40.0	37.0	41.0	32.0	41.0
50-51	37.58528126463375	40.0	37.0	41.0	31.0	41.0
52-53	37.46884966485088	40.0	36.0	41.0	31.0	41.0
54-55	37.33187819216488	39.5	36.0	41.0	31.0	41.0
56-57	37.16031557571078	39.0	36.0	41.0	31.0	41.0
58-59	37.304775413164705	39.0	36.0	41.0	31.0	41.0
60-61	37.36320835836074	39.0	36.0	41.0	31.0	41.0
62-63	37.206897177861904	39.0	35.0	41.0	31.0	41.0
64-65	37.00368298858024	39.0	35.0	41.0	31.0	41.0
66-67	36.73773565194914	38.0	35.0	40.0	31.0	41.0
68-69	36.46846060790178	37.5	35.0	40.0	31.0	41.0
70-71	36.23815666523451	37.0	35.0	40.0	31.0	41.0
72-73	36.004440107940226	37.0	35.0	39.0	31.0	41.0
74-75	35.738705359946465	36.0	35.0	39.0	31.0	41.0
76-77	35.490463713054965	36.0	34.0	39.0	31.0	40.5
78-79	35.18719811108276	35.5	34.0	37.5	30.5	39.5
80-81	34.89370542477903	35.0	34.0	37.0	30.0	39.0
82-83	34.620795296825364	35.0	34.0	37.0	30.0	39.0
84-85	34.39066612756221	35.0	34.0	36.0	30.0	38.0
86-87	34.19463776680071	35.0	34.0	36.0	30.0	37.0
88-89	34.04159825476253	35.0	34.0	36.0	30.0	37.0
90-91	33.92034056692795	35.0	34.0	35.0	30.0	36.5
92-93	33.82547287599271	35.0	34.0	35.0	30.0	36.0
94-95	33.79135175035868	35.0	34.0	35.0	30.0	36.0
96-97	33.78555694463227	35.0	34.0	35.0	30.0	36.0
98-99	33.8129392782917	35.0	34.0	35.0	30.0	36.0
100	33.48277861306972	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	33.0
17	284.0
18	930.0
19	2825.0
20	7083.0
21	14725.0
22	24227.0
23	34215.0
24	44707.0
25	55204.0
26	64030.0
27	69699.0
28	79827.0
29	98060.0
30	115915.0
31	134855.0
32	169479.0
33	220116.0
34	298382.0
35	418128.0
36	641691.0
37	1182652.0
38	1857385.0
39	1290939.0
40	76637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.81255804451734	20.758037852631762	14.402733688494546	24.026670414356353
2	13.8877547620048	21.059891075524156	42.46552101338302	22.586833149088022
3	17.79741612250309	26.37828291096967	30.40870584451241	25.415595122014828
4	12.10235249629457	18.203658923534093	40.05462161132304	29.639366968848297
5	13.146646680221169	38.163268647355096	35.9012757296388	12.788808942784936
6	29.206928975967937	38.308120944128035	19.66018693051175	12.824763149392279
7	26.240004752225072	32.6818921389794	23.764544633970004	17.313558474825523
8	23.792362536818878	37.25402526503072	21.74867394085508	17.204938257295318
9	25.006758881082813	17.76972861607382	21.889849797813106	35.333662705030264
10-11	23.639856679846364	27.242419983685963	29.509854347199305	19.607868989268372
12-13	24.345657283159724	24.755247430046403	29.376683338352393	21.522411948441484
14-15	21.654729595417464	26.334252773242877	26.71279368904328	25.29822394229638
16-17	21.537224888590618	29.390669864570125	27.38093996223136	21.6911652846079
18-19	21.578330572162542	29.094677188600027	28.384690820228187	20.942301419009244
20-21	22.5242564064802	28.16419705699371	28.087770677666075	21.22377585886001
22-23	22.22970404821419	28.103790791030136	27.870481107035634	21.796024053720043
24-25	21.985450168962313	28.201858367749434	27.986965183690092	21.82572627959816
26-27	21.919212014151984	28.375170677027533	27.829200296369187	21.876417012451295
28-29	21.87088210815121	28.444646298858256	27.86712889582291	21.817342697167625
30-31	22.15798053966267	28.19959210517594	27.836865962212006	21.80556139294939
32-33	21.92488761356386	28.322148601717483	27.85480824682709	21.89815553789157
34-35	22.088155280416572	28.29865271610067	27.88295112243385	21.730240881048907
36-37	22.005731531521327	28.18751991851731	27.906069843837354	21.90067870612401
38-39	21.878296576188966	28.287071912632577	28.00866209903996	21.825969412138495
40-41	22.114105757940248	28.11423191505381	27.943301686565704	21.828360640440238
42-43	21.92454634788403	28.192077606386846	27.903707096154236	21.979668949574886
44-45	21.953114836508675	28.2249188581773	27.907781120825152	21.914185184488876
46-47	21.933969463295508	28.161253639803114	27.821306514715676	22.083470382185702
48-49	21.849734090630484	28.196233845496305	27.909787244103057	22.04424481977015
50-51	21.86140128666558	28.26464722749779	27.84321339100771	22.030738094828923
52-53	21.984116601888186	28.177202574987504	27.782014301115183	22.056666522009124
54-55	21.99091014559918	28.07899062065678	27.88261362278647	22.04748561095757
56-57	21.96827874787616	28.135696387996784	27.877813589097155	22.018211275029902
58-59	21.9596920706287	28.20644392336028	27.878116006845687	21.955747999165332
60-61	22.015448483475218	28.169925225779302	27.875764736348042	21.938861554397437
62-63	21.978808758244988	28.24341967204571	27.79518079079847	21.982590778910833
64-65	21.930810495472027	28.222960722734207	27.815479155040286	22.03074962675348
66-67	21.98205574676454	28.23187933743194	27.761937364549894	22.024127551253624
68-69	21.951497963412518	28.171067459567965	27.8304569838987	22.046977593120822
70-71	21.96460076610447	27.98257426510371	27.9071355330118	22.145689435780017
72-73	21.969510275403923	28.026528524763254	27.890876763893928	22.113084435938895
74-75	21.836978796122953	28.170140852701238	27.916533752084447	22.07634659909136
76-77	22.020689262380415	28.085309047579056	27.812522570220093	22.081479119820433
78-79	21.934871034986124	28.097079117096595	27.817195784851933	22.15085406306534
80-81	21.9233819898078	28.16569514734765	27.855707428338295	22.055215434506263
82-83	21.95200223815759	28.121287431119203	27.961482615901183	21.965227714822024
84-85	22.05302183681709	28.12960158522587	27.859334170225296	21.958042407731742
86-87	21.86890923336064	28.22202019811577	27.902048207279705	22.00702236124388
88-89	21.974250444599107	28.11731191019676	27.89740803256654	22.01102961263759
90-91	21.932729262752602	28.26542929782011	27.865130047479745	21.936711391947544
92-93	21.967820808113718	28.268104115023938	27.89262130208772	21.871453774774626
94-95	21.8082653408429	28.30567243470022	27.963844412111154	21.922217812345725
96-97	21.85096183393154	28.445299545622294	27.978120637685716	21.725617982760447
98-99	22.926144788720265	30.41407264245165	28.154849356081872	18.504933212746213
100	21.88587581663927	31.33377971254116	22.04153412658229	24.738810344237276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1789.0
1	1374.0
2	1054.0
3	1140.5
4	1124.5
5	1210.5
6	1429.0
7	1704.0
8	1973.5
9	2241.0
10	2546.0
11	2814.5
12	3094.5
13	3520.5
14	4125.5
15	4942.0
16	5998.0
17	7388.5
18	9079.5
19	11294.5
20	14242.5
21	17984.5
22	22995.5
23	30005.5
24	39396.0
25	51173.0
26	65889.0
27	82718.0
28	100512.5
29	119834.0
30	137926.0
31	155127.5
32	172870.0
33	188485.5
34	204495.0
35	218840.0
36	233108.0
37	247754.5
38	257084.5
39	262962.5
40	269995.0
41	277325.0
42	281473.0
43	285762.0
44	289743.5
45	292221.5
46	292715.0
47	291841.5
48	289394.5
49	282456.5
50	273656.5
51	264860.5
52	253451.0
53	238808.0
54	223927.5
55	207959.5
56	190835.5
57	172704.0
58	154214.5
59	134986.5
60	112905.5
61	91746.5
62	73331.0
63	57289.5
64	43809.0
65	33342.0
66	25266.5
67	19290.5
68	15112.0
69	11625.0
70	8966.0
71	6837.5
72	5163.0
73	3934.5
74	3009.5
75	2292.5
76	1701.5
77	1269.0
78	984.0
79	778.5
80	594.0
81	429.5
82	334.0
83	241.5
84	170.0
85	134.5
86	115.0
87	88.5
88	51.5
89	27.5
90	18.0
91	14.5
92	14.0
93	10.0
94	7.5
95	7.0
96	4.0
97	3.5
98	2.0
99	2.5
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	2.752813302752958E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.2315217407052708E-4
14-15	2.8976982134241662E-5
16-17	1.1590792853696665E-4
18-19	9.634846559635354E-4
20-21	5.073323292018604E-4
22-23	0.0
24-25	1.0160080781350853E-4
26-27	1.6705420821897988E-4
28-29	0.0
30-31	0.0
32-33	5.8414772044384125E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	5.418774924913197E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.9101460358739316E-4
76-77	3.5103124643209646E-5
78-79	5.058748488698889E-4
80-81	3.5914567145964525E-5
82-83	2.7250287899291658E-5
84-85	4.960696128975895E-4
86-87	1.4866837734412865E-4
88-89	2.8212087186634242E-5
90-91	9.526623960716775E-6
92-93	6.276999547862894E-4
94-95	3.6249902517154043E-4
96-97	2.8979998005376686E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2158.0
20-21	4193.0
22-23	4758.0
24-25	5297.0
26-27	7115.0
28-29	10220.0
30-31	16278.0
32-33	19334.0
34-35	25936.0
36-37	38036.0
38-39	43971.0
40-41	43348.0
42-43	46349.0
44-45	52680.0
46-47	54748.0
48-49	55178.0
50-51	53564.0
52-53	52498.0
54-55	56286.0
56-57	58275.0
58-59	58426.0
60-61	58278.0
62-63	56058.0
64-65	58418.0
66-67	61721.0
68-69	63043.0
70-71	61870.0
72-73	60231.0
74-75	60810.0
76-77	62794.0
78-79	64884.0
80-81	65068.0
82-83	62014.0
84-85	61593.0
86-87	63124.0
88-89	67499.0
90-91	70423.0
92-93	72389.0
94-95	85249.0
96-97	220183.0
98-99	407432.0
100-101	4410301.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.56442837285073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.7848895704864	76.82392752618196
2	7.18161796176097	12.289820713805494
3	1.7525202705935055	4.49860185495501
4	0.613924186670653	2.1012028838696692
5	0.26834632275576364	1.148044985627671
6	0.14229952392208176	0.7305466453273035
7	0.07867678847410345	0.47123541023988474
8	0.04906972123337528	0.33588981181991134
9	0.03127869093873591	0.24087089793816036
>10	0.09591468203782852	1.2253215806648072
>50	0.0011052190384455293	0.06466108038197103
>100	3.049100774008357E-4	0.0390863656187105
>500	5.2152010704930186E-5	0.03079024356937768
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4488491067120833E-5
2	0.0	0.0	0.0	0.0	1.4488491067120833E-5
3	0.0	0.0	0.0	0.0	1.4488491067120833E-5
4	0.0	4.34654732013625E-5	0.0	1.4488491067120833E-5	2.8976982134241666E-5
5	2.8976982134241666E-5	4.34654732013625E-5	0.0	1.4488491067120833E-5	2.8976982134241666E-5
6	2.8976982134241666E-5	4.34654732013625E-5	0.0	1.4488491067120833E-5	5.795396426848333E-5
7	2.8976982134241666E-5	5.795396426848333E-5	0.0	5.795396426848333E-5	5.795396426848333E-5
8	2.8976982134241666E-5	7.244245533560416E-5	0.0	7.244245533560416E-5	5.795396426848333E-5
9	2.8976982134241666E-5	1.4488491067120833E-4	0.0	1.8835038387257083E-4	7.244245533560416E-5
10-11	7.244245533560416E-5	1.4488491067120833E-4	0.0	2.0283887493969166E-4	7.244245533560416E-5
12-13	7.244245533560416E-5	1.4488491067120833E-4	0.0	2.4630434814105416E-4	7.244245533560416E-5
14-15	7.244245533560416E-5	2.8976982134241666E-4	0.0	3.042583124095375E-4	1.2315217407052708E-4
16-17	7.244245533560416E-5	3.042583124095375E-4	0.0	3.1150255794309793E-4	1.303964196040875E-4
18-19	7.244245533560416E-5	3.259910490102187E-4	0.0	3.549680311444604E-4	1.5937340173832916E-4
20-21	7.244245533560416E-5	3.549680311444604E-4	0.0	3.9843350434582293E-4	1.5937340173832916E-4
22-23	8.6930946402725E-5	4.7812020521498743E-4	0.0	4.7087595968142704E-4	1.811061383390104E-4
24-25	1.2315217407052708E-4	5.722953971512728E-4	0.0	6.954475712218E-4	2.0283887493969166E-4
26-27	1.303964196040875E-4	6.08516624819075E-4	0.0	0.0013908951424436	2.173273660068125E-4
28-29	1.303964196040875E-4	6.302493614197561E-4	0.0	0.00487537724408616	2.245716115403729E-4
30-31	1.303964196040875E-4	6.954475712218E-4	0.0	0.016951534548531374	2.3181585707393332E-4
32-33	1.303964196040875E-4	7.026918167553604E-4	0.0	0.04773957806616314	2.4630434814105416E-4
34-35	1.6661764727188958E-4	8.041112542252061E-4	0.0	0.08161367018109165	2.680370847417354E-4
36-37	2.0283887493969166E-4	9.05530691695052E-4	0.0	0.12090645795512334	2.752813302752958E-4
38-39	2.0283887493969166E-4	9.272634282957333E-4	0.0	0.15898945672505044	3.042583124095375E-4
40-41	2.173273660068125E-4	9.707289014970957E-4	0.0	0.19220432249642497	3.187468034766583E-4
42-43	2.173273660068125E-4	9.997058836313374E-4	0.0	0.22110161792979746	3.187468034766583E-4
44-45	2.3181585707393332E-4	0.001130102303235425	0.0	0.24363122153917036	3.259910490102187E-4
46-47	2.535485936746146E-4	0.0012315217407052707	0.0	0.2608870144001113	3.549680311444604E-4
48-49	2.60792839208175E-4	0.0012315217407052707	0.0	0.27366586352131184	3.622122766780208E-4
50-51	2.60792839208175E-4	0.0013908951424436	0.0	0.2832137791345445	3.7670076774514165E-4
52-53	2.60792839208175E-4	0.0014053836335107207	0.0	0.2904362919315042	4.274104864800646E-4
54-55	2.60792839208175E-4	0.001412627879044281	0.0	0.2964779927064936	4.926086962821083E-4
56-57	2.60792839208175E-4	0.0014343606156449625	0.0	0.3022806333788755	5.2158567841635E-4
58-59	2.60792839208175E-4	0.001441604861178523	0.0	0.3086700579394758	5.2158567841635E-4
60-61	2.60792839208175E-4	0.001463337597779204	0.0	0.31377725104063586	5.2158567841635E-4
62-63	2.60792839208175E-4	0.0014923145799134456	0.0	0.31842805667318164	5.2158567841635E-4
64-65	2.60792839208175E-4	0.0017675959101887415	0.0	0.32170245565435096	5.2158567841635E-4
66-67	2.60792839208175E-4	0.0018183056289236645	0.0	0.3247305502873792	5.2158567841635E-4
68-69	2.60792839208175E-4	0.001847282611057906	0.0	0.32788904134001157	5.2158567841635E-4
70-71	2.60792839208175E-4	0.0018545268565914666	0.0	0.33043177152229125	5.505626605505916E-4
72-73	2.60792839208175E-4	0.0018545268565914666	0.0	0.33292379198583605	6.230051158861958E-4
74-75	2.60792839208175E-4	0.0018835038387257082	0.0	0.33507533290930347	6.230051158861958E-4
76-77	2.60792839208175E-4	0.0019342135574606312	0.0	0.33725585081490517	6.230051158861958E-4
78-79	2.60792839208175E-4	0.0019631905395948728	0.0	0.3389582485152919	6.230051158861958E-4
80-81	2.60792839208175E-4	0.0019704347851284333	0.0	0.3398203137337856	6.230051158861958E-4
82-83	2.60792839208175E-4	0.0019849232761955543	0.0	0.34029843393900056	6.664705890875583E-4
84-85	2.60792839208175E-4	0.001999411767262675	0.0	0.3405085170594738	6.664705890875583E-4
86-87	2.60792839208175E-4	0.002013900258329796	0.0	0.3405592267782087	6.737148346211187E-4
88	2.60792839208175E-4	0.0021877621511352456	0.0	0.34056647102374227	6.809590801546791E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5610	0.0	35.267063	1
GTATCAA	9035	0.0	31.256086	1
TCAACGC	9060	0.0	28.97675	4
ATCAACG	9215	0.0	28.535227	3
CAACGCA	9260	0.0	28.350903	5
AACGCAG	9825	0.0	26.677324	6
TATCAAC	10720	0.0	25.396704	2
ACGCAGA	11820	0.0	22.210443	7
CGCAGAG	12085	0.0	21.618471	8
GTACATG	20050	0.0	19.651142	1
TACATGG	19745	0.0	19.376608	2
ACATGGG	20210	0.0	18.32416	3
TATACCG	600	0.0	16.910105	5
GCAGAGT	15810	0.0	16.498194	9
CATGGGG	15175	0.0	16.157925	4
GGACCGA	1115	0.0	15.924198	6
ACCGAAC	960	0.0	15.853108	8
AGGACCG	1400	0.0	15.098306	5
AGAGTAC	13240	0.0	14.687726	10-11
CAGAGTA	15340	0.0	14.578573	10-11
>>END_MODULE