FastQCFastQC Report
Fri 27 May 2016
SRR2140317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20896006
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157700.035.9854051
GTATCAA264700.030.0530011
TCAACGC266050.028.0208364
ATCAACG277250.026.9500333
CAACGCA277450.026.8084015
AACGCAG293500.025.429036
TATCAAC308650.024.6614482
ACGCAGA350000.021.2030167
CGCAGAG355750.020.8603128
GTACATG594300.020.6381261
TACATGG597700.019.9109672
ACATGGG604250.019.0288123
CATGGGG455050.017.4910144
GCAGAGT464000.015.9297189
AGAGTAC392950.015.01348610-11
TATACCG15150.014.546865
CAGAGTA452050.014.17571310-11
GGACCGA28500.013.6811156
ATGGGGG275100.013.3569425
GAGTACA446650.013.1514421
Produced by FastQC (version 0.11.5)