##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140317_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20896006
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79426676083458	33.0	31.0	34.0	30.0	34.0
2	31.947543803346917	33.0	31.0	34.0	30.0	34.0
3	31.993118302129126	34.0	31.0	34.0	30.0	34.0
4	35.522588192212424	37.0	35.0	37.0	33.0	37.0
5	35.57424179529811	37.0	35.0	37.0	33.0	37.0
6	35.62202250516199	37.0	35.0	37.0	33.0	37.0
7	35.514260428524	37.0	35.0	37.0	33.0	37.0
8	35.47803876970556	37.0	35.0	37.0	33.0	37.0
9	37.10257855017844	39.0	37.0	39.0	33.0	39.0
10-11	37.078349781293134	39.0	37.0	39.0	33.0	39.0
12-13	37.02863020808857	39.0	37.0	39.0	33.0	39.0
14-15	38.39040599433213	40.0	38.0	41.0	33.0	41.0
16-17	38.40671301013218	40.0	38.0	41.0	33.0	41.0
18-19	38.41851067136945	40.0	38.0	41.0	33.0	41.0
20-21	38.47299697370621	40.0	38.0	41.0	33.0	41.0
22-23	38.45513746207156	40.0	38.0	41.0	33.0	41.0
24-25	38.46550937401729	40.0	38.0	41.0	33.0	41.0
26-27	38.44457751856889	40.0	38.0	41.0	33.0	41.0
28-29	38.42051367560682	40.0	38.0	41.0	33.0	41.0
30-31	38.37102213359038	40.0	38.0	41.0	33.0	41.0
32-33	38.3310487395973	40.0	38.0	41.0	33.0	41.0
34-35	38.278880626930814	40.0	38.0	41.0	33.0	41.0
36-37	38.220742134635046	40.0	38.0	41.0	33.0	41.0
38-39	38.14243968988305	40.0	38.0	41.0	33.0	41.0
40-41	38.07678665591934	40.0	38.0	41.0	33.0	41.0
42-43	37.99231877628839	40.0	37.5	41.0	32.5	41.0
44-45	37.872084197908336	40.0	37.0	41.0	32.0	41.0
46-47	37.77015217656975	40.0	37.0	41.0	32.0	41.0
48-49	37.6735971196159	40.0	37.0	41.0	32.0	41.0
50-51	37.541274903463076	40.0	36.5	41.0	31.0	41.0
52-53	37.42177615171765	40.0	36.0	41.0	31.0	41.0
54-55	37.28455096538745	39.0	36.0	41.0	31.0	41.0
56-57	37.10901502678381	39.0	35.5	41.0	31.0	41.0
58-59	37.256055471904546	39.0	36.0	41.0	31.0	41.0
60-61	37.31542210319638	39.0	35.5	41.0	31.0	41.0
62-63	37.15517432319217	39.0	35.0	41.0	31.0	41.0
64-65	36.95526593333322	39.0	35.0	40.5	31.0	41.0
66-67	36.695147540344365	38.0	35.0	40.0	31.0	41.0
68-69	36.430101323307426	37.5	35.0	40.0	31.0	41.0
70-71	36.20368441174027	37.0	35.0	40.0	31.0	41.0
72-73	35.9757761264827	36.5	35.0	39.0	31.0	41.0
74-75	35.71259033718773	36.0	34.0	39.0	31.0	41.0
76-77	35.47083231178076	36.0	34.0	39.0	31.0	40.5
78-79	35.16868530967017	35.0	34.0	37.5	30.5	39.5
80-81	34.87435866857639	35.0	34.0	37.0	30.0	39.0
82-83	34.602339769292726	35.0	34.0	37.0	30.0	39.0
84-85	34.371347468035054	35.0	34.0	36.0	30.0	38.0
86-87	34.17217649307507	35.0	34.0	36.0	30.0	37.0
88-89	34.01629752339582	35.0	34.0	36.0	30.0	37.0
90-91	33.89488207659573	35.0	34.0	35.0	30.0	36.5
92-93	33.793568779400616	35.0	34.0	35.0	30.0	36.0
94-95	33.76093425274623	35.0	34.0	35.0	30.0	36.0
96-97	33.75573256284676	35.0	34.0	35.0	30.0	36.0
98-99	33.78522717287081	35.0	34.0	35.0	30.0	36.0
100	33.44995520878938	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	10.0
16	107.0
17	792.0
18	2645.0
19	8151.0
20	21299.0
21	43201.0
22	71553.0
23	101652.0
24	134880.0
25	168793.0
26	197780.0
27	214902.0
28	243889.0
29	297602.0
30	354422.0
31	412851.0
32	519687.0
33	678601.0
34	923508.0
35	1298108.0
36	2002983.0
37	3662123.0
38	5553224.0
39	3770784.0
40	212458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53098951062705	20.952707421695802	14.54210436195319	23.974198705723957
2	13.852623319499429	21.127927509209176	42.3863727833922	22.6330763878992
3	17.618768869036504	26.492665631891565	30.668951760446472	25.21961373862546
4	12.11766975947461	18.21592604826013	40.09638014077906	29.570024051486204
5	13.095302871877612	38.24071719820189	35.94937227402724	12.714607655893257
6	29.173713866659494	38.41663808863761	19.596228102155024	12.81341994254787
7	26.020139925304385	32.93733261753466	23.826830830733872	17.21569662642708
8	23.84376800135107	37.207961465928335	21.736178132839868	17.212092399880724
9	25.0216668199655	17.729474235411303	21.922457334669602	35.32640160995359
10-11	23.58897313176713	27.24633639750083	29.59930865225786	19.565381818474176
12-13	24.207963646977248	24.83060496950155	29.470863748475246	21.490567635045956
14-15	21.58385168221454	26.420730247999174	26.728425544854357	25.26699252493193
16-17	21.482523184419627	29.486582911974445	27.405362375022936	21.625531528582993
18-19	21.535891936874453	29.15925658026799	28.422725318331523	20.882126164526042
20-21	22.44702593462314	28.23026153142738	28.12865039705906	21.194062136890416
22-23	22.190085511262836	28.14375077313515	27.929218045719388	21.73694566988262
24-25	21.918699527658323	28.284338068192444	27.99796396679561	21.798998437353625
26-27	21.860421652645844	28.42809627732614	27.86195494808064	21.849527121947368
28-29	21.83689134230094	28.481536257470175	27.9171371053743	21.764435294854586
30-31	22.08052803044587	28.325038609842146	27.859805439548328	21.734627920163653
32-33	21.87108243593586	28.411229968280022	27.872329665704175	21.84535793007994
34-35	22.044363686712483	28.332333268618992	27.9300029525413	21.69330009212722
36-37	22.010360150597354	28.222051722347224	27.90946340049187	21.858124726563553
38-39	21.868047542885375	28.351839181409982	28.03125420176607	21.748859073938576
40-41	22.078454208359968	28.175974447927604	27.96316258955268	21.782408754159743
42-43	21.870213376706577	28.25623695237443	27.931951687594726	21.941597983324268
44-45	21.96250400423412	28.289538886832755	27.90441755850777	21.843539550425355
46-47	21.892179884043685	28.23061119639054	27.821308431768	22.055900487797775
48-49	21.868221093773865	28.25901850707442	27.907001641648627	21.96575875750309
50-51	21.84968972560824	28.27617787797797	27.892072130972018	21.98206026544177
52-53	21.90660030967101	28.234257918767586	27.845553821671352	22.01358794989005
54-55	21.944723027714737	28.144077821080295	27.910967812545145	22.00023133865983
56-57	21.918905949342644	28.201196074973428	27.903519619114803	21.976378356569125
58-59	21.89473122726075	28.308421491256563	27.928139607716385	21.868707673766306
60-61	22.022919979967607	28.20001369964149	27.900505953880185	21.87656036651072
62-63	21.99018195345056	28.292486943195644	27.822878022323483	21.894453081030317
64-65	21.923705287116423	28.22466484849702	27.862164787414777	21.989465076971776
66-67	21.983677997701623	28.259280677610764	27.770394264615888	21.986647060071725
68-69	21.89790827149678	28.16431096433999	27.8910493097969	22.046731454366327
70-71	21.914605526357885	27.998049642586086	27.926957323475932	22.160387507580097
72-73	21.978792720691168	28.03270315747798	27.88304746822019	22.105456653610666
74-75	21.784109821962677	28.17118801817568	27.9591127215468	22.085589438314848
76-77	21.92619930012472	28.15956112645449	27.860573731928156	22.05366584149263
78-79	21.862166841248012	28.141801800876415	27.81817654434456	22.177854813531013
80-81	21.91704288837448	28.193005668996385	27.856368148794395	22.03358329383474
82-83	21.859582522300585	28.20666593762163	28.014146698012617	21.919604842065173
84-85	21.988889402427045	28.202636346568944	27.88785590571512	21.920618345288894
86-87	21.85304375148981	28.231698572398162	27.93191815530209	21.983339520809935
88-89	21.89009082899711	28.21589348461374	27.901542110003607	21.992473576385542
90-91	21.908880407788946	28.33490374122683	27.864973969166666	21.891241881817557
92-93	21.90738474254018	28.26784990493535	27.964931178270213	21.859834174254257
94-95	21.741215132759777	28.36136215478649	27.979602939899728	21.917819772554008
96-97	21.791836021902082	28.51708027469355	28.01773149068532	21.673352212719042
98-99	22.89895001700784	30.41132938858598	28.191815868694842	18.497904725711344
100	21.850989228409222	31.46401151320168	21.99757389953182	24.687425358857276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5769.0
1	4354.0
2	3288.5
3	3647.5
4	3606.0
5	3803.0
6	4472.0
7	5335.5
8	6075.0
9	6965.0
10	8029.0
11	8872.0
12	9822.0
13	11249.0
14	13105.5
15	15514.0
16	18624.5
17	22539.5
18	27548.5
19	34250.5
20	43114.0
21	55531.0
22	73147.0
23	96522.5
24	126948.5
25	166449.0
26	214136.5
27	265304.0
28	318914.5
29	378773.5
30	434355.0
31	484277.5
32	535950.5
33	580826.5
34	623374.0
35	663849.0
36	706576.5
37	747807.5
38	772932.5
39	787007.5
40	805997.0
41	826003.0
42	837270.5
43	846967.5
44	856544.0
45	868134.0
46	871325.5
47	865104.0
48	856858.0
49	841230.0
50	820117.0
51	794655.0
52	758623.0
53	710938.5
54	665586.5
55	621495.5
56	576031.5
57	525249.0
58	468409.5
59	410301.0
60	342807.5
61	276914.5
62	220310.5
63	172249.0
64	132455.5
65	100881.5
66	76649.0
67	59288.0
68	46544.5
69	35629.0
70	27412.0
71	21084.0
72	15964.0
73	11786.5
74	8786.5
75	6737.0
76	5081.5
77	3892.5
78	3072.5
79	2383.0
80	1789.5
81	1331.0
82	1041.5
83	748.0
84	528.0
85	400.0
86	316.5
87	241.0
88	148.5
89	92.5
90	66.0
91	54.0
92	49.5
93	35.5
94	21.5
95	16.5
96	15.5
97	13.5
98	8.5
99	16.0
100	68.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.1964008815847393E-4
6	0.0
7	0.0
8	2.3928017631694782E-5
9	0.0
10-11	4.546323350022009E-5
12-13	1.2921129521115184E-4
14-15	4.3070431737050617E-5
16-17	1.0767607934262653E-4
18-19	0.0010504399740314011
20-21	4.2133312049626913E-4
22-23	0.0
24-25	8.869403050101412E-5
26-27	1.7513749132769526E-4
28-29	0.0
30-31	0.0
32-33	6.028989019088792E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	3.317172578863354E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.7426041665208545E-4
76-77	8.659607124016313E-6
78-79	4.7856926000236017E-4
80-81	4.132113466537127E-5
82-83	3.8810736339382096E-5
84-85	4.075416826085904E-4
86-87	1.3739751519341724E-4
88-89	3.090145954082718E-5
90-91	1.565086354109114E-5
92-93	6.345535003954061E-4
94-95	3.9916313517276503E-4
96-97	3.4151697769559185E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	6720.0
20-21	12941.0
22-23	14284.0
24-25	16380.0
26-27	21527.0
28-29	30121.0
30-31	47604.0
32-33	58545.0
34-35	76982.0
36-37	111352.0
38-39	126038.0
40-41	125855.0
42-43	135841.0
44-45	154656.0
46-47	161237.0
48-49	161376.0
50-51	157296.0
52-53	156676.0
54-55	167790.0
56-57	174570.0
58-59	174709.0
60-61	173634.0
62-63	168918.0
64-65	176568.0
66-67	185161.0
68-69	188980.0
70-71	185688.0
72-73	178061.0
74-75	178939.0
76-77	184629.0
78-79	193709.0
80-81	194021.0
82-83	186376.0
84-85	185579.0
86-87	192375.0
88-89	204306.0
90-91	213118.0
92-93	220603.0
94-95	260446.0
96-97	674564.0
98-99	1247834.0
100-101	1.3409997E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.90384391280836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.60816640850463	70.87837420600998
2	8.068527224129703	13.055497342944689
3	2.2280760861628486	5.407797597023402
4	0.9148077620704959	2.9604585757110766
5	0.4298019285521941	1.7386314070505362
6	0.24034680595763236	1.1666988284482995
7	0.13962621212161183	0.7907408090136488
8	0.09417320070201717	0.6095179144292456
9	0.06169970715703649	0.4492569129568978
>10	0.21021435985195597	2.61027624167838
>50	0.0036437398026905534	0.19635922954031598
>100	8.69149651273779E-4	0.10928218341726427
>500	4.741533593294501E-5	0.027108751776269015
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.785603526338957E-6	0.0
2	0.0	1.4356810579016871E-5	0.0	1.4356810579016871E-5	0.0
3	0.0	1.9142414105355828E-5	0.0	2.3928017631694785E-5	0.0
4	0.0	5.7427242316067484E-5	0.0	2.8713621158033742E-5	0.0
5	4.785603526338957E-6	5.7427242316067484E-5	0.0	3.3499224684372696E-5	0.0
6	6.699844936874539E-5	5.7427242316067484E-5	0.0	3.8284828210711656E-5	9.571207052677914E-6
7	6.699844936874539E-5	5.7427242316067484E-5	0.0	3.8284828210711656E-5	1.4356810579016871E-5
8	6.699844936874539E-5	6.699844936874539E-5	0.0	5.7427242316067484E-5	1.9142414105355828E-5
9	6.699844936874539E-5	1.3878250226382976E-4	0.0	7.178405289508435E-5	3.3499224684372696E-5
10-11	8.853366523727069E-5	1.3878250226382976E-4	0.0	1.0289047581628757E-4	3.3499224684372696E-5
12-13	1.1246168286896548E-4	1.3878250226382976E-4	0.0	1.3160409697432132E-4	4.067762997388113E-5
14-15	1.2921129521115184E-4	1.9620974457989723E-4	0.0	1.4835370931650765E-4	6.460564760557592E-5
16-17	1.9381694281672774E-4	2.0578095163257515E-4	0.0	1.770673304745414E-4	8.135525994776227E-5
18-19	2.0099534810623617E-4	2.225305639747615E-4	0.0	2.105665551589141E-4	1.1964008815847392E-4
20-21	2.0099534810623617E-4	2.5124418513279524E-4	0.0	2.3688737455377837E-4	1.435681057901687E-4
22-23	2.1295935692208358E-4	2.9431461686984584E-4	0.0	2.919218151066764E-4	1.5553211460601608E-4
24-25	2.321017710274394E-4	3.34992246843727E-4	0.0	5.216307843709464E-4	1.7467452871137193E-4
26-27	2.5124418513279524E-4	3.445634538964049E-4	0.0	0.0010025839387680115	1.8185293400088036E-4
28-29	2.5842259042230367E-4	3.445634538964049E-4	0.0	0.0037854123893341146	1.866385375272193E-4
30-31	2.7277940100132054E-4	3.684914715280997E-4	0.0	0.013368583450827877	1.866385375272193E-4
32-33	2.8235060805399843E-4	3.732770750544386E-4	0.0	0.03439652534556125	1.9142414105355828E-4
34-35	3.0627862568569325E-4	4.3548992089684506E-4	0.0	0.05665676014832691	2.0099534810623617E-4
36-37	3.325994450805575E-4	5.00095568502421E-4	0.0	0.081831427498633	2.0578095163257515E-4
38-39	3.3738504860689644E-4	5.072739737919294E-4	0.0	0.10489085809029725	2.0578095163257515E-4
40-41	3.4934905742274385E-4	5.55130009055319E-4	0.0	0.1250597841520528	2.2013776221159202E-4
42-43	3.517418591859133E-4	5.790580266870138E-4	0.0	0.14372842350830106	2.225305639747615E-4
44-45	3.6131306623859126E-4	6.508420795820981E-4	0.0	0.15802062843971235	2.2492336573793096E-4
46-47	3.684914715280997E-4	6.939125113191488E-4	0.0	0.1700492429031653	2.2970896926426994E-4
48-49	3.7088427329126915E-4	6.963053130823182E-4	0.0	0.17859633080120668	2.536369868959647E-4
50-51	3.780626785807776E-4	8.20731004767131E-4	0.0	0.18569338083076736	2.536369868959647E-4
52-53	3.85241083870286E-4	8.279094100566395E-4	0.0	0.191476782692348	2.656009957118121E-4
54-55	3.876338856334555E-4	8.374806171093175E-4	0.0	0.19631981346100302	2.775650045276595E-4
56-57	3.876338856334555E-4	8.518374276883343E-4	0.0	0.2009474920709728	2.79957806290829E-4
58-59	3.876338856334555E-4	8.566230312146734E-4	0.0	0.20564456193207448	2.919218151066764E-4
60-61	3.948122909229639E-4	8.661942382673512E-4	0.0	0.2100162107533851	2.967074186330153E-4
62-63	4.3548992089684506E-4	8.805510488463681E-4	0.0	0.21417011461424734	3.014930221593543E-4
64-65	4.45061127949523E-4	9.954055334785031E-4	0.0	0.2177641028625279	3.014930221593543E-4
66-67	4.522395332390314E-4	0.0010025839387680115	0.0	0.22117384537504442	3.0627862568569325E-4
68-69	4.546323350022009E-4	0.001040868766978723	0.0	0.22435387891829664	3.1106422921203217E-4
70-71	4.7377474910755674E-4	0.001045654370505062	0.0	0.22705774491067815	3.3020664331738806E-4
72-73	4.929171632129125E-4	0.0010480471722682314	2.3928017631694785E-6	0.2293596202068472	3.780626785807776E-4
74-75	4.929171632129125E-4	0.0010695823881367568	4.785603526338957E-6	0.23137435929143588	3.780626785807776E-4
76-77	5.00095568502421E-4	0.0011413664410318413	4.785603526338957E-6	0.2332790294949188	3.85241083870286E-4
78-79	5.024883702655905E-4	0.001174865665716214	4.785603526338957E-6	0.23466924731932026	3.876338856334555E-4
80-81	5.024883702655905E-4	0.0011772584674793834	4.785603526338957E-6	0.23540623026237645	4.1156190326515024E-4
82-83	5.072739737919294E-4	0.0011772584674793834	4.785603526338957E-6	0.2359135042361684	4.498467314758619E-4
84-85	5.168451808446073E-4	0.0011820440710057223	4.785603526338957E-6	0.2361144995842746	4.570251367653704E-4
86-87	5.216307843709464E-4	0.0011964008815847393	4.785603526338957E-6	0.23618389083540653	4.594179385285399E-4
88	5.264163878972853E-4	0.0012633993309534846	4.785603526338957E-6	0.23618867643893288	4.594179385285399E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15770	0.0	35.985405	1
GTATCAA	26470	0.0	30.053001	1
TCAACGC	26605	0.0	28.020836	4
ATCAACG	27725	0.0	26.950033	3
CAACGCA	27745	0.0	26.808401	5
AACGCAG	29350	0.0	25.42903	6
TATCAAC	30865	0.0	24.661448	2
ACGCAGA	35000	0.0	21.203016	7
CGCAGAG	35575	0.0	20.860312	8
GTACATG	59430	0.0	20.638126	1
TACATGG	59770	0.0	19.910967	2
ACATGGG	60425	0.0	19.028812	3
CATGGGG	45505	0.0	17.491014	4
GCAGAGT	46400	0.0	15.929718	9
AGAGTAC	39295	0.0	15.013486	10-11
TATACCG	1515	0.0	14.54686	5
CAGAGTA	45205	0.0	14.175713	10-11
GGACCGA	2850	0.0	13.681115	6
ATGGGGG	27510	0.0	13.356942	5
GAGTACA	44665	0.0	13.151442	1
>>END_MODULE