FastQCFastQC Report
Fri 27 May 2016
SRR2140316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8653086
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94800.039.2222751
GTATCAA160750.031.7792261
ATCAACG175650.027.8892173
TCAACGC176150.027.8567524
CAACGCA178250.027.5747175
AACGCAG184650.026.7080756
TATCAAC195650.025.5428352
ACGCAGA213950.022.9927857
CGCAGAG219100.022.4523338
TGCGCGA6101.891749E-1020.39964994
TACATGG209300.020.2610592
GTACATG215750.019.9984991
ACATGGG210250.019.4456773
GCAGAGT263000.018.626399
GAGTACT147550.017.91076512-13
AGAGTAC229750.016.94514710-11
CAGAGTA251050.016.7034910-11
CATGGGG157900.016.5671464
ATTCGCG3106.284289E-515.79462490-91
GTACTTT175050.015.07426414-15
Produced by FastQC (version 0.11.5)