##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140316_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8653086
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15434967363089	33.0	31.0	34.0	30.0	34.0
2	32.37573762701538	34.0	31.0	34.0	31.0	34.0
3	32.42985739422907	34.0	31.0	34.0	30.0	34.0
4	36.06525972352522	37.0	35.0	37.0	35.0	37.0
5	35.918860854959725	37.0	35.0	37.0	35.0	37.0
6	35.94087716220548	37.0	35.0	37.0	35.0	37.0
7	35.89275456178293	37.0	35.0	37.0	35.0	37.0
8	35.89442240606415	37.0	35.0	37.0	35.0	37.0
9	37.67034084718446	39.0	37.0	39.0	35.0	39.0
10-11	37.5595419368304	39.0	37.0	39.0	35.0	39.0
12-13	37.52821034021851	39.0	37.0	39.0	35.0	39.0
14-15	38.81151839933175	40.0	38.0	41.0	35.0	41.0
16-17	38.799229026499916	40.0	38.0	41.0	35.0	41.0
18-19	38.77469500476477	40.0	38.0	41.0	34.0	41.0
20-21	38.7659403428423	40.0	38.0	41.0	34.0	41.0
22-23	38.69048652292595	40.0	38.0	41.0	34.0	41.0
24-25	38.63385251969544	40.0	38.0	41.0	34.0	41.0
26-27	38.470836053349885	40.0	38.0	41.0	34.0	41.0
28-29	38.34539105955953	40.0	38.0	41.0	34.0	41.0
30-31	38.226715423542835	40.0	38.0	41.0	34.0	41.0
32-33	38.15152815488348	40.0	38.0	41.0	33.5	41.0
34-35	38.20488265922869	40.0	38.0	41.0	34.0	41.0
36-37	38.16692259031905	40.0	38.0	41.0	33.0	41.0
38-39	38.11174058750605	40.0	38.0	41.0	33.0	41.0
40-41	38.050323823916926	40.0	37.5	41.0	33.0	41.0
42-43	38.02451811054973	40.0	37.0	41.0	33.0	41.0
44-45	38.06348300319485	40.0	37.0	41.0	33.0	41.0
46-47	38.05649302158146	40.0	37.0	41.0	33.0	41.0
48-49	37.98450261983261	40.0	37.0	41.0	33.0	41.0
50-51	37.90340007476992	40.0	37.0	41.0	33.0	41.0
52-53	37.680135056233155	40.0	37.0	41.0	32.0	41.0
54-55	37.56646208532272	40.0	36.0	41.0	32.0	41.0
56-57	37.50958161511902	39.0	36.0	41.0	32.0	41.0
58-59	37.356335961876965	39.0	36.0	41.0	32.0	41.0
60-61	37.169543906853676	39.0	35.0	41.0	31.0	41.0
62-63	36.925042631452555	39.0	35.0	40.0	31.0	41.0
64-65	36.637960796339314	38.0	35.0	40.0	31.0	41.0
66-67	36.36668009419313	38.0	35.0	40.0	31.0	41.0
68-69	36.090204543575666	37.0	34.5	40.0	30.0	41.0
70-71	35.79706914300267	37.0	34.0	39.0	30.0	41.0
72-73	35.49268173645589	36.0	34.0	39.0	30.0	41.0
74-75	35.14178000172214	36.0	34.0	39.0	29.5	40.0
76-77	33.77951042825721	34.5	32.0	36.5	28.5	39.0
78-79	34.499115679059884	35.0	34.0	37.0	29.0	39.0
80-81	34.43559732423835	35.0	34.0	37.0	30.0	39.0
82-83	34.14650989367875	35.0	34.0	36.5	29.0	39.0
84-85	33.954266972397185	35.0	34.0	36.0	29.0	37.0
86-87	33.708133411654785	35.0	33.0	36.0	29.0	37.0
88-89	33.58185616238771	35.0	33.0	35.0	29.0	37.0
90-91	33.38992827269496	35.0	33.0	35.0	29.0	36.0
92-93	33.274821561756895	35.0	33.0	35.0	29.0	36.0
94-95	33.26156308512552	35.0	33.0	35.0	29.0	36.0
96-97	33.28653446238071	35.0	33.0	35.0	29.0	35.5
98-99	33.36827605447826	35.0	33.0	35.0	29.5	35.0
100	32.104616528046414	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	11.0
20	51.0
21	258.0
22	1188.0
23	4056.0
24	10883.0
25	23422.0
26	42929.0
27	68759.0
28	99842.0
29	134146.0
30	172320.0
31	215444.0
32	269653.0
33	340518.0
34	453078.0
35	625219.0
36	962061.0
37	1661056.0
38	2214199.0
39	1299866.0
40	54127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.35458725361102	20.803745623237766	13.814713039948984	24.026954083202224
2	14.803747472289079	21.671470733100307	40.83509628819129	22.689685506419327
3	18.05708391202861	26.28275045457771	30.840754385198526	24.819411248195152
4	12.375018577187376	18.162814977223153	39.55318368498823	29.908982760601248
5	12.82209607069663	38.201192037152985	36.245589145883905	12.731122746266477
6	29.55565216848648	38.63131604146775	18.978489292721694	12.834542497324076
7	26.19496674365654	32.82892369265716	23.67637395491042	17.299735608775872
8	24.20205924221717	36.96046705187028	21.54559656520229	17.291877140710262
9	25.17164396609487	16.80363514242202	21.772301812324528	36.252419079158585
10-11	23.808563788687643	27.00738210622199	29.504884153468485	19.679169951621883
12-13	24.484328481191568	24.48020278545712	29.674627063685723	21.360841669665596
14-15	21.58273360509765	26.141407816818184	27.007780807910613	25.26807777017355
16-17	21.325716689510436	29.269569558695384	27.74507164005926	21.659642111734918
18-19	21.30396306749647	28.65782800522242	29.21062523095094	20.827583696330183
20-21	22.02251626843647	27.832381985251693	29.093511214939717	21.051590531372124
22-23	21.828108611457175	27.7521250705968	28.87470377085741	21.545062547088616
24-25	21.61349091580722	27.80423454002106	29.00341099826545	21.57886354590627
26-27	21.700216001909116	27.98203920932636	28.790308335697944	21.527436453066578
28-29	21.554241022061593	28.180862773689082	28.786219088328274	21.47867711592105
30-31	21.77045835296475	27.98708808376917	28.77338651200724	21.469067051258843
32-33	21.58598627069755	28.090711757657527	28.737053915619143	21.586248056025784
34-35	21.70833871940836	28.108749003917012	28.749777641785062	21.433134634889566
36-37	21.742305332734478	28.074250453886073	28.635313421067497	21.548130792311948
38-39	21.599453186505364	28.19534650048434	28.69393615778147	21.511264155228822
40-41	21.822010086856313	28.04693403207461	28.56949983321031	21.561556047858765
42-43	21.706212715969407	28.127211662106372	28.47075650929623	21.695819112627987
44-45	21.820134680624356	28.27106417320749	28.280755712768435	21.628045433399723
46-47	21.773934573940906	28.23277621611886	28.058883255261385	21.934405954678848
48-49	21.86857076731692	28.241819664398314	28.09970806006927	21.789901508215493
50-51	21.76528832773936	28.330512509799682	28.042034152131578	21.862165010329377
52-53	21.851946043674513	28.295660026626322	27.92385195793785	21.928541971761312
54-55	21.860634352111504	28.243598087262356	27.960351862036788	21.93541569858936
56-57	21.91474369034065	28.300636673571606	27.91953058878302	21.865089047304725
58-59	21.867500872914263	28.374679931436553	27.952010877040767	21.805808318608417
60-61	21.97131345119678	28.2826043994141	27.93317800595343	21.812904143435688
62-63	21.931683279384455	28.37477051824345	27.829102244770347	21.864443957601747
64-65	21.857199513089203	28.293935623539934	27.932501569571045	21.916363293799822
66-67	21.939933952866458	28.297488053092472	27.776917398437178	21.985660595603886
68-69	21.9114390497334	28.29598582800511	27.861889634363234	21.93068548789826
70-71	21.844872966705296	28.1072889803826	27.92912515100449	22.118712901907607
72-73	21.913283994798448	28.153568184299417	27.909007726506275	22.024140094395868
74-75	21.836324449877004	28.231273760676434	27.9039185864961	22.02848320295046
76-77	21.895320004823198	28.186828270733315	27.8161316452541	22.10172007918939
78-79	21.832084991695133	28.17016844031166	27.850964573362642	22.14678199463056
80-81	21.851824761146336	28.238797010635057	27.87303466561697	22.036343562601637
82-83	21.877715877116856	28.209717581377085	27.937028274843023	21.975538266663037
84-85	22.006117807440503	28.14081809582388	27.86950636501985	21.983557731715766
86-87	21.800475532502013	28.228152145483016	27.935401512547564	22.035970809467408
88-89	21.937606555725807	28.151369201793173	27.895407265562188	22.015616976918828
90-91	21.937298642578156	28.219829369435693	27.88330797872167	21.959564009264476
92-93	21.948224099879056	28.195392347461485	27.903216456065426	21.953167096594033
94-95	21.76839936967789	28.29049124207939	27.95560828648253	21.985501101760182
96-97	21.781454407884596	28.48157058360174	28.042080169338572	21.694894839175088
98-99	22.875888710917973	30.368964654225582	28.264539946561655	18.490606688294793
100	29.050017447287736	41.827936008800016	29.122046543912244	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1517.0
1	1351.0
2	1842.5
3	2676.5
4	3603.5
5	5887.0
6	7951.0
7	9211.5
8	9759.5
9	9323.5
10	8468.0
11	7694.0
12	8344.0
13	11121.0
14	17305.0
15	25997.5
16	33407.5
17	38035.0
18	40285.5
19	40729.0
20	40109.5
21	39815.0
22	41469.5
23	46858.5
24	56555.5
25	70725.0
26	89858.5
27	112282.5
28	135734.5
29	160397.0
30	183124.0
31	203054.5
32	222669.0
33	242264.0
34	261756.0
35	281391.0
36	300288.0
37	317472.0
38	330482.5
39	338341.0
40	346411.5
41	355374.5
42	361875.5
43	366082.0
44	370772.5
45	375168.0
46	376932.0
47	374912.0
48	368513.5
49	359765.0
50	350490.0
51	337459.5
52	320261.5
53	302099.0
54	280576.0
55	257223.5
56	233309.5
57	208439.0
58	184005.5
59	157069.0
60	130687.5
61	106881.5
62	85181.0
63	66017.5
64	49503.5
65	37455.0
66	28437.5
67	21635.5
68	16414.5
69	12131.0
70	9160.5
71	6851.0
72	4972.5
73	3548.0
74	2449.0
75	1667.0
76	1156.5
77	802.0
78	542.0
79	365.0
80	242.5
81	159.0
82	105.5
83	66.0
84	40.5
85	28.0
86	22.5
87	19.0
88	13.0
89	8.0
90	6.5
91	5.0
92	4.0
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0012249964925807972
18-19	4.044799739653576E-5
20-21	0.0
22-23	0.0063166130332474035
24-25	0.008410448378055145
26-27	0.020817356494107358
28-29	0.01585693388127335
30-31	0.0019481253641863822
32-33	0.0061254014522960365
34-35	0.032072406758970544
36-37	0.005648458958156145
38-39	0.0028219693634911744
40-41	0.00774940471705966
42-43	0.007794594901609856
44-45	0.008474795822799412
46-47	0.005461109693778942
48-49	0.0010451574351310834
50-51	0.0026389149844743835
52-53	0.0010551172804422438
54-55	0.005506023602746343
56-57	0.0011634097185888362
58-59	0.0013688695392603356
60-61	8.964717014658249E-4
62-63	0.001556128331061837
64-65	3.6258892322309536E-4
66-67	0.00521277321888954
68-69	0.004043971496799305
70-71	0.0015686688701850562
72-73	0.0014589898787787838
74-75	7.493073090417603E-4
76-77	7.365852541082858E-4
78-79	9.849340900014863E-4
80-81	0.0010135799287574335
82-83	0.006450001825327508
84-85	8.007130232863673E-4
86-87	3.942661713001353E-5
88-89	4.1635276737273956E-4
90-91	3.5832941614130686E-4
92-93	0.0025046100997987604
94-95	0.003402546950281087
96-97	4.351474514545152E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	214.0
20-21	773.0
22-23	1526.0
24-25	2928.0
26-27	5445.0
28-29	12132.0
30-31	26854.0
32-33	34190.0
34-35	46294.0
36-37	68821.0
38-39	79275.0
40-41	77925.0
42-43	89517.0
44-45	97977.0
46-47	96965.0
48-49	90290.0
50-51	82855.0
52-53	81076.0
54-55	85822.0
56-57	89261.0
58-59	87627.0
60-61	84997.0
62-63	81061.0
64-65	84410.0
66-67	88517.0
68-69	89676.0
70-71	88598.0
72-73	84058.0
74-75	85076.0
76-77	85458.0
78-79	90317.0
80-81	91742.0
82-83	87850.0
84-85	89441.0
86-87	94300.0
88-89	102882.0
90-91	109324.0
92-93	117160.0
94-95	141461.0
96-97	314822.0
98-99	1778722.0
100-101	3705447.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.91790301977804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.49687387461277	75.99886856257673
2	7.247302151069598	12.308514024391139
3	1.840270991793448	4.688158608336804
4	0.6712230863320082	2.2799542779911035
5	0.3057858258810566	1.2983345553495154
6	0.15490787691649285	0.7892671241396673
7	0.08862216028314854	0.5267925608629344
8	0.05066070433153489	0.3441600621871143
9	0.03467494123155241	0.26500709670457234
>10	0.10765589155800726	1.344148885672698
>50	0.001697719811644171	0.09478892658871824
>100	3.0324450119429525E-4	0.04840183390413155
>500	2.1531677540350414E-5	0.013603481294871092
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1556570684724502E-5	0.0
2	0.0	1.1556570684724502E-5	0.0	2.3113141369449005E-5	0.0
3	0.0	1.1556570684724502E-5	0.0	2.3113141369449005E-5	0.0
4	0.0	2.3113141369449005E-5	0.0	2.3113141369449005E-5	0.0
5	1.1556570684724502E-5	2.3113141369449005E-5	0.0	2.3113141369449005E-5	0.0
6	1.1556570684724502E-4	2.3113141369449005E-5	0.0	3.466971205417351E-5	2.3113141369449005E-5
7	1.1556570684724502E-4	2.3113141369449005E-5	0.0	4.622628273889801E-5	2.3113141369449005E-5
8	1.1556570684724502E-4	3.466971205417351E-5	0.0	4.622628273889801E-5	2.3113141369449005E-5
9	1.1556570684724502E-4	8.089599479307152E-5	0.0	6.933942410834701E-5	3.466971205417351E-5
10-11	1.2134399218960727E-4	8.089599479307152E-5	0.0	1.3290056287433178E-4	4.622628273889801E-5
12-13	1.271222775319695E-4	8.089599479307152E-5	0.0	1.6179198958614303E-4	4.622628273889801E-5
14-15	1.271222775319695E-4	1.0978742150488278E-4	0.0	1.6757027492850528E-4	9.245256547779602E-5
16-17	1.5023541890141852E-4	1.1556570684724502E-4	0.0	2.137965576674033E-4	9.245256547779602E-5
18-19	1.5023541890141852E-4	1.2134399218960727E-4	0.0	2.7735769643338806E-4	9.245256547779602E-5
20-21	1.5023541890141852E-4	1.3867884821669403E-4	0.0	3.813668325959085E-4	9.245256547779602E-5
22-23	1.5023541890141852E-4	1.6757027492850528E-4	0.0	4.4492797136189334E-4	9.245256547779602E-5
24-25	1.5023541890141852E-4	1.8490513095559204E-4	0.0	4.969325394431536E-4	9.823085082015826E-5
26-27	1.5023541890141852E-4	1.9646170164031653E-4	0.0	5.604936782091384E-4	1.1556570684724502E-4
28-29	1.5023541890141852E-4	1.9646170164031653E-4	0.0	0.001132543927103001	1.1556570684724502E-4
30-31	1.5023541890141852E-4	2.3113141369449005E-4	0.0	0.002629119830774824	1.1556570684724502E-4
32-33	1.560137042437808E-4	2.3113141369449005E-4	0.0	0.0051426739547024035	1.1556570684724502E-4
34-35	1.6179198958614303E-4	3.120274084875616E-4	0.0	0.008580753733407942	1.1556570684724502E-4
36-37	1.6179198958614303E-4	3.9292340328063306E-4	0.0	0.01370609283208326	1.1556570684724502E-4
38-39	1.6179198958614303E-4	4.160365446500821E-4	0.0	0.021061850072910407	1.3290056287433176E-4
40-41	1.6179198958614303E-4	4.911542541007913E-4	0.0	0.028770082719621646	1.5023541890141852E-4
42-43	1.6179198958614303E-4	5.373805368396893E-4	0.0	0.036368527944828005	1.7334856027086752E-4
44-45	1.8490513095559204E-4	6.47167958344572E-4	0.0	0.044377231429342086	1.9646170164031653E-4
46-47	2.0801827232504104E-4	7.165073824529191E-4	0.0	0.05373227539862657	1.9646170164031653E-4
48-49	2.0801827232504104E-4	7.165073824529191E-4	0.0	0.06283885309818948	2.1957484300976553E-4
50-51	2.2535312835212778E-4	8.551862306696132E-4	0.0	0.07144271997296686	2.1957484300976553E-4
52-53	2.3113141369449005E-4	8.667428013543376E-4	0.0	0.07951498459624692	2.1957484300976553E-4
54-55	2.369096990368523E-4	8.725210866966999E-4	0.0	0.08794550291075345	2.1957484300976553E-4
56-57	2.4268798437921454E-4	8.898559427237867E-4	0.0	0.09661293092429683	2.1957484300976553E-4
58-59	2.4268798437921454E-4	8.956342280661488E-4	0.0	0.10493366181729846	2.1957484300976553E-4
60-61	2.54244555063939E-4	9.418605108050469E-4	0.0	0.11353752869207587	2.1957484300976553E-4
62-63	3.120274084875615E-4	9.476387961474092E-4	0.0	0.12134977047494963	2.4268798437921454E-4
64-65	3.120274084875615E-4	0.0011094307857335522	0.0	0.1286593014330379	2.484662697215768E-4
66-67	3.120274084875615E-4	0.001149878783130088	0.0	0.13631552951166787	2.54244555063939E-4
68-69	3.120274084875615E-4	0.0011729919244995368	0.0	0.14396597930495547	2.54244555063939E-4
70-71	3.23583979172286E-4	0.0011961050658689859	0.0	0.1502354189014185	2.54244555063939E-4
72-73	3.9292340328063306E-4	0.0012076616365537104	0.0	0.1550776220183181	2.7735769643338806E-4
74-75	3.9292340328063306E-4	0.0012307747779231595	0.0	0.15907619547523277	2.7735769643338806E-4
76-77	4.1603654465008203E-4	0.0012596662046349709	0.0	0.16295342493995785	2.7735769643338806E-4
78-79	4.275931153348066E-4	0.0012827793460044197	0.0	0.16529363050361454	2.7735769643338806E-4
80-81	4.275931153348066E-4	0.0012827793460044197	0.0	0.1660679207394911	2.7735769643338806E-4
82-83	4.275931153348066E-4	0.001294335916689144	0.0	0.16663997098838496	2.7735769643338806E-4
84-85	4.275931153348066E-4	0.0013058924873738686	0.0	0.16679020640728637	2.7735769643338806E-4
86-87	4.275931153348066E-4	0.0013058924873738686	0.0	0.16685954583139473	2.7735769643338806E-4
88	4.275931153348066E-4	0.0013636753407974913	0.0	0.16686532411673707	2.7735769643338806E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9480	0.0	39.222275	1
GTATCAA	16075	0.0	31.779226	1
ATCAACG	17565	0.0	27.889217	3
TCAACGC	17615	0.0	27.856752	4
CAACGCA	17825	0.0	27.574717	5
AACGCAG	18465	0.0	26.708075	6
TATCAAC	19565	0.0	25.542835	2
ACGCAGA	21395	0.0	22.992785	7
CGCAGAG	21910	0.0	22.452333	8
TGCGCGA	610	1.891749E-10	20.399649	94
TACATGG	20930	0.0	20.261059	2
GTACATG	21575	0.0	19.998499	1
ACATGGG	21025	0.0	19.445677	3
GCAGAGT	26300	0.0	18.62639	9
GAGTACT	14755	0.0	17.910765	12-13
AGAGTAC	22975	0.0	16.945147	10-11
CAGAGTA	25105	0.0	16.70349	10-11
CATGGGG	15790	0.0	16.567146	4
ATTCGCG	310	6.284289E-5	15.794624	90-91
GTACTTT	17505	0.0	15.074264	14-15
>>END_MODULE